Evaluation of Staph ID 32 system and Staph-Zym system for identification of coagulase-negative staphylococci.


:The purpose of the investigation was to evaluate two commercially available identification systems: a new modification of the Staph-Zym system (Rosco, Tåstrup, Denmark) and the Staph ID 32 API system (API System, BioMérieux, Paris, France). A local standard method to be used in routine laboratories was also evaluated. A total of 200 staphylococcal isolates, including strains from both the American Type Culture Collection and the Czechoslovak Collection of Microorganisms as well as 89 clinical isolates, were used in tests of all three identification systems. The Staph ID 32 API system identified from 50 to 100% of the reference strains and 82.1% of the clinical isolates correctly. The Staph-Zym system identified from 90 to 100% of the reference strains and 82.1% of the clinical isolates correctly. Most misidentifications were of minor importance, but in both systems major failures appeared (Staphylococcus aureus was identified as a coagulase-negative staphylococcus). Both systems needed backup from a reference laboratory to determine if two isolates were of the same strain.


J Clin Microbiol


Renneberg J,Rieneck K,Gutschik E




Has Abstract


1995-05-01 00:00:00












  • Infection caused by thymidine-requiring, trimethoprim-resistant bacteria.

    abstract::We first noted the appearance of thymidine-requiring, gram-negative bacilli in clinical specimens 2 years ago. Since then we have seen 10 patients colonized or infected with these organisms. These strains do not grow on Mueller-Hinton media, growth on MacConkey agar is variable, and growth in API 20E (Analytab Product...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: King CH,Shlaes DM,Dul MJ

    更新日期:1983-07-01 00:00:00

  • Fully automated, internally controlled quantification of hepatitis B Virus DNA by real-time PCR by use of the MagNA Pure LC and LightCycler instruments.

    abstract::We report on the development of a fully automated real-time PCR assay for the quantitative detection of hepatitis B virus (HBV) DNA in plasma with EDTA (EDTA plasma). The MagNA Pure LC instrument was used for automated DNA purification and automated preparation of PCR mixtures. Real-time PCR was performed on the Light...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Leb V,Stöcher M,Valentine-Thon E,Hölzl G,Kessler H,Stekel H,Berg J

    更新日期:2004-02-01 00:00:00

  • Long-chain fatty acids of peptococci and peptostreptococci.

    abstract::The long-chain fatty acids extracted from the whole cells of 12 clinically significant species of peptococci and peptostreptococci were characterized by gas-liquid chromatography. The resulting methylated fatty acid profiles (and some unidentified compounds) of 82 strains allowed the 12 species to be separated into fo...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Wells CL,Field CR

    更新日期:1976-12-01 00:00:00

  • Isolation of Salmonella enteritidis, serotype Horsham, from three American Indian Tribes.

    abstract::Salmonella enteritidis, serotype Horsham, identified rarely worldwide, was isolated from the stools of six American Indians with gastroenteritis. The patients were from separate tribes located in three geographically distinct villages. Although significant contact was demonstrated between ill individuals within two tr...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Castle SP,Nims LJ,Lapham SC

    更新日期:1983-07-01 00:00:00

  • Diagnosis of Toxoplasma parasitemia in patients with AIDS by gene detection after amplification with polymerase chain reaction.

    abstract::We sought evidence of toxoplasma parasitemia among 37 people with active or dormant Toxoplasma gondii infection or no evidence of infection. DNA was extracted from erythrocyte-free portions of blood samples, and the T. gondii B1 gene was amplified by the polymerase chain reaction. Evidence of T. gondii parasitemia was...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Filice GA,Hitt JA,Mitchell CD,Blackstad M,Sorensen SW

    更新日期:1993-09-01 00:00:00

  • Propionibacterium acnes types I and II represent phylogenetically distinct groups.

    abstract::Although two phenotypes of the opportunistic pathogen Propionibacterium acnes (types I and II) have been described, epidemiological investigations of their roles in different infections have not been widely reported. Using immunofluorescence microscopy with monoclonal antibodies (MAbs) QUBPa1 and QUBPa2, specific for ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: McDowell A,Valanne S,Ramage G,Tunney MM,Glenn JV,McLorinan GC,Bhatia A,Maisonneuve JF,Lodes M,Persing DH,Patrick S

    更新日期:2005-01-01 00:00:00

  • Updating the PGMY primers and probes for improved detection of HPV68a: validation of version 2 of the PGMY-CHUV assay.

    abstract::HPV68a is not efficiently detected by PCR with the PGMY primers. Version 2 of the PGMY-CHUV assay (PGv2) was developed from version 1 (PGv1) to evaluate HPV68-discordant results with the Anyplex II HPV28 assay. We now report that PGv2 is significantly more sensitive than PGv1 for HPV68a and as sensitive and specific f...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Estrade C,Sahli R

    更新日期:2014-11-01 00:00:00

  • Rapid PCR-based molecular pathotyping of H5 and H7 avian influenza viruses.

    abstract::While the majority of avian influenza virus (AIV) subtypes are classified as low-pathogenicity avian influenza viruses (LPAIV), the H5 and H7 subtypes have the ability to mutate to highly pathogenic avian influenza viruses (HPAIV) in poultry and therefore are the etiological agents of notifiable AIV (NAIV). It is of g...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Leijon M,Ullman K,Thyselius S,Zohari S,Pedersen JC,Hanna A,Mahmood S,Banks J,Slomka MJ,Belák S

    更新日期:2011-11-01 00:00:00

  • Euparal as a permanent mounting medium for helminth eggs and proglottids.

    abstract::The importance of euparal as a permanent mounting medium for eggs of parasites and the process of making permanent slides are analyzed and discussed in detail. Studies show that helminth eggs mounted in euparal exhibit excellent optical and drying properties. Euparal also can be used to identify proglottids of Taenia ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Berlin OG,Miller MJ

    更新日期:1980-11-01 00:00:00

  • Evaluation of the Binax NOW, BD Directigen, and BD Directigen EZ assays for detection of respiratory syncytial virus.

    abstract::The Binax NOW assay (Binax, Inc., Portland, Maine) and the BD Directigen EZ assay (Becton Dickinson and Company, Sparks, Md.), two new rapid immunoassays for detection of respiratory syncytial virus (RSV), as well as the BD Directigen RSV assay (DRSV) (Becton Dickinson and Company) and direct immunofluorescence staini...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Ohm-Smith MJ,Nassos PS,Haller BL

    更新日期:2004-07-01 00:00:00

  • Serratia rubidaea as an invasive pathogen.

    abstract::Serratia rubidaea biotype 1 was isolated from the bile and blood of a patient with a bile tract carcinoma obstructing the common bile duct and who underwent invasive procedures. The infection was cleared after adequate treatment with antibiotics. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Ursua PR,Unzaga MJ,Melero P,Iturburu I,Ezpeleta C,Cisterna R

    更新日期:1996-01-01 00:00:00

  • Immunodiagnosis of Echinococcus infections: confirmatory testing and species differentiation by a new commercial Western Blot.

    abstract::The Echinococcus Western Blot IgG (LDBIO Diagnostics, Lyon, France), using a whole larval antigen from Echinococcus multilocularis, was evaluated for serodiagnosis and differentiation between two human parasitic infections of worldwide importance: cystic echinococcosis, due to Echinococcus granulosus, and alveolar ech...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Liance M,Janin V,Bresson-Hadni S,Vuitton DA,Houin R,Piarroux R

    更新日期:2000-10-01 00:00:00

  • Relationship between photochromogenicity and test temperature in mycobacteria.

    abstract::Although it has been recommended that photochromogenicity of mycobacteria should be tested after incubation at 25 to 30 degrees C for 2 weeks (Wayne et al., Int. J. Syst. Bacteriol. 24:412-419, 1974), our results indicate that photochromogenicity should be determined at 37 degrees C in the dark and under continuous ex...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Tsukamura M

    更新日期:1981-08-01 00:00:00

  • Reversed passive latex agglutination assay for detection of toxigenic Corynebacterium diphtheriae.

    abstract::A reversed passive latex agglutination (RPLA) assay for determining the toxigenicity of Corynebacterium diphtheriae is presented. Rabbit antitoxin antiserum was raised by using commercially available diphtheria toxoid. This antiserum reacted with the diphtheria toxin when the culture supernatant was assayed by Western...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Toma C,Sisavath L,Iwanaga M

    更新日期:1997-12-01 00:00:00

  • Comparative cost-effectiveness of two-tiered testing strategies for serodiagnosis of lyme disease with noncutaneous manifestations.

    abstract::The mainstay of laboratory diagnosis for Lyme disease is two-tiered serological testing, in which a reactive first-tier enzyme-linked immunosorbent assay (ELISA) or an immunofluorescence assay is supplemented by separate IgM and IgG immunoblots. Recent data suggest that the C6 ELISA can be substituted for immunoblots ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Wormser GP,Levin A,Soman S,Adenikinju O,Longo MV,Branda JA

    更新日期:2013-12-01 00:00:00

  • Use of the BD PHOENIX Automated Microbiology System for direct identification and susceptibility testing of gram-negative rods from positive blood cultures in a three-phase trial.

    abstract::The present study describes the use of the automated BACTEC 9240 blood culture system, the Serum Separator Tube (SST), and the BD PHOENIX Automated Microbiology System in combination for the direct identification and antimicrobial susceptibility testing (AST) of gram-negative rods (GNRs) from positive blood cultures (...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Funke G,Funke-Kissling P

    更新日期:2004-04-01 00:00:00

  • Improved detection times for Mycobacterium avium complex and Mycobacterium tuberculosis with the BACTEC radiometric system.

    abstract::A total of 2,559 routine clinical specimens were cultured for mycobacteria by using BACTEC Middlebrook 7H12 medium (BACTEC), Lowenstein-Jensen slants (LJ), and Mycobactosel selective Middlebrook 7H11 slants (M7H11). Thirty-three isolates (1.3%) of M. avium complex and 82 isolates (3.2%) of M. tuberculosis were recover...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Kirihara JM,Hillier SL,Coyle MB

    更新日期:1985-11-01 00:00:00

  • Comparison of broth microdilution and E-test for susceptibility testing of Neisseria meningitidis.

    abstract::The susceptibilities of 54 clinical isolates of Neisseria meningitidis to penicillin, cefotaxime, ceftriaxone, cefepime, imipenem, ciprofloxacin, chloramphenicol, and rifampin were determined by the microdilution method in both cation-adjusted Mueller-Hinton broth (CAMHB) and Haemophilus test medium (HTM). Poor growth...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Pascual A,Joyanes P,Martinez-Martinez L,Suarez AI,Perea EJ

    更新日期:1996-03-01 00:00:00

  • Evaluation of the solid-phase radioimmunoassay for diagnosis of St. Louis encephalitis infection in humans.

    abstract::Since the use of highly purified viral proteins in the solid-phase radioimmunoassay (SPRIA) procedure is not practical for routine diagnostic serology, the procedure was adapted for use with the crude antigens now commonly used to diagnose St. Louis encephalitis (SLE) virus infections. SLE and various other alpha- and...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Wolff KL,Muth DJ,Hudson BW,Trent DW

    更新日期:1981-08-01 00:00:00

  • Lessons in public (mis)communication about the laboratory from the COVID-19 pandemic.

    abstract::The COVID-19 pandemic has put the clinical laboratory in the spotlight. The news media is regularly seeking out interviews with microbiologists, infectious disease specialists, and pathologists. Increased public exposure offers opportunities to improve how laboratory professionals communicate our insights. We can emph...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Mazer BL

    更新日期:2021-01-21 00:00:00

  • Diphtheria in the Republic of Georgia: use of molecular typing techniques for characterization of Corynebacterium diphtheriae strains.

    abstract::Sixty-six Corynebacterium diphtheriae strains (62 of the gravis biotype and 4 of the mitis biotype) isolated during the Georgian diphtheria epidemic of 1993 to 1998 and 13 non-Georgian C. diphtheriae strains (10 Russian and 3 reference isolates) were characterized by (i) biotyping, (ii) toxigenicity testing with the E...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Sulakvelidze A,Kekelidze M,Gomelauri T,Deng Y,Khetsuriani N,Kobaidze K,De Zoysa A,Efstratiou A,Morris JG Jr,Imnadze P

    更新日期:1999-10-01 00:00:00

  • Inhibition of Pseudomonas aeruginosa slime production by staphylococcal extracellular product.

    abstract::Staphylococcus aureus produced an inhibitory factor that suppressed slime formation by Psuudomonas aeruginosa but had little effect on the growth of the organism. The inhibitory factor was not found in broth cultures but could be extracted from cultures grown on solid agar. The inhibitory factor moderately inhibited g...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Shibl AM,Al-Sowaygh IA

    更新日期:1980-02-01 00:00:00

  • Rapid identification of Staphylococcus aureus in blood cultures by use of the direct tube coagulase test.

    abstract::Direct tube coagulase testing for identification of Staphylococcus aureus from BACTEC culture broth showed a sensitivity, a specificity, and positive and negative predicative values of 34%, 100%, 100%, and 80.2% with 2 h of incubation and 65%, 98.7, 99.7%, and 88.6% with 4 h of incubation. Anaerobic blood culture cont...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Qian Q,Eichelberger K,Kirby JE

    更新日期:2007-07-01 00:00:00

  • Multicenter evaluation of a commercial PCR-enzyme-linked immunosorbent assay diagnostic kit (Onychodiag) for diagnosis of dermatophytic onychomycosis.

    abstract::We prospectively evaluated a new PCR-enzyme-linked immunosorbent assay kit (Onychodiag; BioAdvance, France) for the diagnosis of dermatophytic onychomycosis by testing nail samples from 438 patients with suspected onychomycosis and from 108 healthy controls in three independent laboratories. In two laboratories, sampl...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,多中心研究


    authors: Savin C,Huck S,Rolland C,Benderdouche M,Faure O,Noacco G,Menotti J,Candolfi E,Pelloux H,Grillot R,Coupe S,Derouin F

    更新日期:2007-04-01 00:00:00

  • Medium-dependent zone size discrepancies associated with susceptibility testing of group D streptococci against various cephalosporins.

    abstract::Mueller-Hinton (MH) agar media from various commercial sources, either supplemented or not supplemented with 5% sheep blood, were studied to determine their effect on disk diffusion susceptibility testing results obtained with 90 strains of group D streptococci and four cephalosporins. The cephalosporins investigated ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Sahm DF,Baker CN,Jones RN,Thornsberry C

    更新日期:1983-10-01 00:00:00

  • Development of real-time PCR assays for genotyping of Chlamydia trachomatis.

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    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Jalal H,Stephen H,Alexander S,Carne C,Sonnex C

    更新日期:2007-08-01 00:00:00

  • Preparation and properties of a national reference endotoxin.

    abstract::A large pool of refined endotoxin was prepared from Escherichia coli O113 by extraction with hot acqueous phenol. It was characterized chemically and biologically and will be available for a reference standard designated as reference endotoxin EC. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Rudbach JA,Akiya FI,Elin RJ,Hochstein HD,Luoma MK,Milner EC,Milner KC,Thomas KR

    更新日期:1976-01-01 00:00:00

  • Metabolically deficient dwarf-colony mutants of Escherichia coli: deficiency and resistance to antibiotics of strains isolated from urine culture.

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    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Borderon E,Horodniceanu T

    更新日期:1978-12-01 00:00:00

  • Survival of Acinetobacter baumannii on dry surfaces: comparison of outbreak and sporadic isolates.

    abstract::Acinetobacter spp. are important nosocomial pathogens reported with increasing frequency in outbreaks of cross-infection during the past 2 decades. The majority of such outbreaks are caused by Acinetobacter baumannii. To investigate whether desiccation tolerance may be involved in the ability of certain strains of A. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Jawad A,Seifert H,Snelling AM,Heritage J,Hawkey PM

    更新日期:1998-07-01 00:00:00

  • Accurate Detection of Methicillin-Resistant Staphylococcus aureus in Mixtures by Use of Single-Bacterium Duplex Droplet Digital PCR.

    abstract::Accurate and rapid identification of methicillin-resistant Staphylococcus aureus (MRSA) is needed to screen MRSA carriers and improve treatment. The current widely used duplex PCR methods are not able to differentiate MRSA from coexisting methicillin-susceptible S. aureus (MSSA) or other methicillin-resistant staphylo...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Luo J,Li J,Yang H,Yu J,Wei H

    更新日期:2017-10-01 00:00:00