Detection of group B streptococcal antigen in body fluids by a latex-coupled monoclonal antibody assay.

abstract：:Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and conventional standard methods were compared for time to pathogen identification and impact on clinical outcomes in peritoneal dialysis-related peritonitis patients. The MALDI-TOF MS method identified the causative microorga...

journal_title：Journal of clinical microbiology

authors： Lin WH,Hwang JC,Tseng CC,Chang YT,Wu AB,Yan JJ,Wu JJ,Wang MC

更新日期：2016-05-01 00:00:00

abstract：:We report the evaluation of recombinant severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) nucleocapsid protein enzyme-linked immunosorbent assay (ELISA)-based antibody tests for serodiagnosis of SARS-CoV pneumonia and compare the sensitivities and specificities of this ELISA for detection of immunoglobul...

journal_title：Journal of clinical microbiology

authors： Woo PC,Lau SK,Wong BH,Tsoi HW,Fung AM,Chan KH,Tam VK,Peiris JS,Yuen KY

更新日期：2004-05-01 00:00:00

abstract：:Traditional ribotyping detects genomic restriction fragment length polymorphisms by probing chromosomal DNA with rRNA. Although it is a powerful method for determining the molecular epidemiology of bacterial pathogens, technical difficulties limit its application. As an alternative, polymorphisms were sought in the 16...

journal_title：Journal of clinical microbiology

authors： Kostman JR,Edlind TD,LiPuma JJ,Stull TL

更新日期：1992-08-01 00:00:00

abstract：:We report on a reverse line blot (RLB) assay, utilizing fungal species-specific oligonucleotide probes to hybridize with internal transcribed spacer 2 region sequences amplified using a nested panfungal PCR. Reference and clinical strains of 16 Candida species (116 strains), Cryptococcus neoformans (five strains of Cr...

journal_title：Journal of clinical microbiology

authors： Playford EG,Kong F,Sun Y,Wang H,Halliday C,Sorrell TC

更新日期：2006-03-01 00:00:00

abstract：:Carbohydrate antigen detection, nucleic acid probe detection, and bacterial culture are commonly used to confirm group A streptococcus (GAS) pharyngitis. Compared to standard throat swab specimens, the sensitivities of these tests with mouth specimens are poor. When testing for GAS pharyngitis, the throat remains the ...

journal_title：Journal of clinical microbiology

authors： Fox JW,Marcon MJ,Bonsu BK

更新日期：2006-07-01 00:00:00

abstract：:HIV-1 non-B subtypes/circulating recombinant forms (CRFs) are increasing worldwide. Since subtype identification can be clinically relevant, we assessed the added value in HIV-1 subtyping using updated molecular phylogeny (Mphy) and the performance of routinely used automated tools. Updated Mphy (2015 updated referenc...

journal_title：Journal of clinical microbiology

authors： Fabeni L,Berno G,Fokam J,Bertoli A,Alteri C,Gori C,Forbici F,Takou D,Vergori A,Zaccarelli M,Maffongelli G,Borghi V,Latini A,Pennica A,Mastroianni CM,Montella F,Mussini C,Andreoni M,Antinori A,Perno CF,Santoro MM,

更新日期：2017-09-01 00:00:00

abstract：:Cellulosimicrobium funkei is a rare, opportunistic pathogen. We describe a case of bacteremia and possibly prosthetic valve endocarditis by this organism in a nonimmunocompromised patient. Useful phenotypic tests for differentiating C. funkei from Cellulosimicrobium cellulans and Cellulosimicrobium terreum include mot...

journal_title：Journal of clinical microbiology

authors： Petkar H,Li A,Bunce N,Duffy K,Malnick H,Shah JJ

更新日期：2011-03-01 00:00:00

abstract：:A cell-free growth medium for the opportunistic pathogenic ameba Balamuthia mandrillaris is presented. This represents an advance over the use of monkey kidney cells for growth of the amebas and can be helpful in isolation of these amebas from brain tissue from cases in which amebic meningoencephalitis is a diagnostic...

journal_title：Journal of clinical microbiology

authors： Schuster FL,Visvesvara GS

更新日期：1996-02-01 00:00:00

abstract：:Vibrio metschnikovii and Vibrio gazogenes are two new Vibrio species that have been little studied. Thirteen strains of V. metschnikovii were highly related to the type strain, NCTC 8443, by DNA-DNA hybridization. Relatedness values were 83 to 90% at 60 degrees C and 75 to 84% at the more stringent 75 degrees C. Diver...

journal_title：Journal of clinical microbiology

authors： Farmer JJ 3rd,Hickman-Brenner FW,Fanning GR,Gordon CM,Brenner DJ

更新日期：1988-10-01 00:00:00

abstract：:We investigated whether methicillin (meticillin)-resistant Staphylococcus aureus (MRSA) isolates with low-level mupirocin resistance can serve as recipients of a pSK41-like plasmid conferring high-level mupirocin resistance without substantial fitness cost. Our results suggest that acquisition of the plasmid conferrin...

journal_title：Journal of clinical microbiology

authors： Mongkolrattanothai K,Pumfrey L,Mankin P,Gray BM

更新日期：2009-12-01 00:00:00

abstract：:The development and application of a double-label immunofluorescence method which has the potential to screen for single or dual infections from any site, in single shell vial cultures, is described. In this study, a total of 1,141 ocular specimens were inoculated in shell vials, centrifuged at 15,000 X g for 1 h, inc...

journal_title：Journal of clinical microbiology

authors： Walpita P,Darougar S

更新日期：1989-07-01 00:00:00

abstract：:A total of 41 Brucella isolates, examined by standard biotyping procedures, were found to be similar to Brucella abortus biovar 2 in dye sensitivity but had a dominant M antigen. Oxidative metabolic tests performed on 39 of the isolates confirmed them as B. abortus. Additional biochemical and bacteriophage susceptibil...

journal_title：Journal of clinical microbiology

authors： Ewalt DR,Forbes LB

更新日期：1987-04-01 00:00:00

abstract：:The purpose of the present study was to determine the extent to which bacteria not detected by culture contribute to the microbial flora of the bowel of preterm infants with and without neonatal necrotizing enterocolitis (NEC). Fecal samples from 32 preterm infants in special care baby units including samples from 10 ...

journal_title：Journal of clinical microbiology

authors： Millar MR,Linton CJ,Cade A,Glancy D,Hall M,Jalal H

更新日期：1996-10-01 00:00:00

abstract：:Of 90 clinical isolates of Cryptococcus neoformans studied, 3 were determined to be serotype B/C. The patients from whom these B/C isolates were obtained were identified as never having lived in or visited the areas associated with B/C serotypes. This finding suggests a broader geographic distribution of this serotype...

journal_title：Journal of clinical microbiology

authors： Fromtling RA,Shadomy S,Shadomy HJ,Dismukes WE

更新日期：1982-08-01 00:00:00

abstract：:Rapid pathogen identification can alter antibiotic prescribing practices if interpreted correctly. Microbiology reporting can be difficult to understand, and new technology has made it more challenging. Nebraska Medicine recently implemented the BioFire FilmArray blood culture identification panel (BCID) coupled with ...

journal_title：Journal of clinical microbiology

authors： Donner LM,Campbell WS,Lyden E,Van Schooneveld TC

更新日期：2017-05-01 00:00:00

abstract：:The rapid identification of blood culture isolates and antimicrobial susceptibility test (AST) results play critical roles for the optimal treatment of patients with bloodstream infections. Whereas others have looked at the time to detection in automated culture systems, we examined the overall time from specimen coll...

journal_title：Journal of clinical microbiology

authors： Tabak YP,Vankeepuram L,Ye G,Jeffers K,Gupta V,Murray PR

更新日期：2018-11-27 00:00:00

abstract：:This report describes the first well-documented reported case of the recovery of Blastomyces dermatitidis from the blood of a patient with disseminated blastomycosis. The isolate was recovered by using a lysis-centrifugation method and inhibitory mold agar after 6 days of incubation at 30 degrees C. One of six blood s...

journal_title：Journal of clinical microbiology

authors： Musial CE,Wilson WR,Sinkeldam IR,Roberts GD

更新日期：1987-08-01 00:00:00

abstract：:Using the Staphylococcus aureus absorption method, we have modified a commercial radioimmunoassay kit for the determination of total antibody to hepatitis B core antigen so that we can now determine the predominant globulin species of that antibody. One-hundred percent of acute hepatitis B sera had a predominance of i...

journal_title：Journal of clinical microbiology

authors： Schable CA,Maynard JE

更新日期：1982-11-01 00:00:00

abstract：:Three human rotavirus (HRV) VP4 serotypes and one subtype have been described on the basis of a fourfold or an eightfold-or-greater difference in neutralization titer when tested with hyperimmune antisera to recombinant VP4 or VP8* (serotypes P1A, P1B, P2, and P3). To start to analyze the antigenic basis underlying se...

journal_title：Journal of clinical microbiology

authors： Padilla-Noriega L,Werner-Eckert R,Mackow ER,Gorziglia M,Larralde G,Taniguchi K,Greenberg HB

更新日期：1993-03-01 00:00:00

abstract：:By using a single synthetic oligonucleotide primer pair in the polymerase chain reaction, we amplified specific Shiga-like-toxin (SLT) gene segments from DNAs of 20 clinical Escherichia coli isolates, irrespective of whether they produce SLT-I, SLT-II, or heretofore uncategorized SLTs. These segments were not detectab...

journal_title：Journal of clinical microbiology

authors： Karch H,Meyer T

更新日期：1989-12-01 00:00:00

abstract：:To our knowledge, no data are available on whether the microbial species composition and abundance sampled with self-collected vaginal swabs are comparable to those of swabs collected by clinicians. Twenty healthy women were recruited to the study during a routine gynecological visit. Eligible women were between 18 an...

journal_title：Journal of clinical microbiology

authors： Forney LJ,Gajer P,Williams CJ,Schneider GM,Koenig SS,McCulle SL,Karlebach S,Brotman RM,Davis CC,Ault K,Ravel J

更新日期：2010-05-01 00:00:00

abstract：:Forty-two difficult-to-cultivate group A coxsackieviruses (i.e., group A types other than A7, A9, and A16), collected primarily from throat swab specimens of patients suffering from fever, pharyngitis, lymphadenopathy, and cough during the 1986 enterovirus season, were isolated in less than 24-h-old suckling mice. Thi...

journal_title：Journal of clinical microbiology

authors： Lipson SM,Walderman R,Costello P,Szabo K

更新日期：1988-07-01 00:00:00

abstract：:The BD Phoenix system was evaluated for species-level identification of yeasts (250 clinical isolates) and compared with the Vitek 2 system, using ribosomal internal transcribed spacer (ITS) sequence analysis as the gold standard. Considering only the species included in each system's database, 96.3% (236/245) and 91....

journal_title：Journal of clinical microbiology

authors： Posteraro B,Ruggeri A,De Carolis E,Torelli R,Vella A,De Maio F,Ricciardi W,Posteraro P,Sanguinetti M

更新日期：2013-11-01 00:00:00

abstract：:The majority of cervical human papillomavirus (HPV) infections in young women are transient, but whether the clearance differs among different HPV genotypes and the different factors predicting genotype-specific clearance are partly unknown. In the Finnish Family HPV Study, 131 of 252 women (mean age, 25.5 years) clea...

journal_title：Journal of clinical microbiology

authors： Louvanto K,Syrjänen KJ,Rintala MA,Grénman SE,Syrjänen SM

更新日期：2010-08-01 00:00:00

abstract：:The Roche PGMY primer-based research prototype line blot assay (PGMY-LB) is a convenient tool in epidemiological studies for the detection and typing of human papillomavirus (HPV) DNA. This assay has been optimized and is being commercialized as the Linear Array HPV genotyping test (LA-HPV). We assessed the agreement ...

journal_title：Journal of clinical microbiology

authors： Coutlée F,Rouleau D,Petignat P,Ghattas G,Kornegay JR,Schlag P,Boyle S,Hankins C,Vézina S,Coté P,Macleod J,Voyer H,Forest P,Walmsley S,Canadian Women's HIV study Group.,Franco E

更新日期：2006-06-01 00:00:00

abstract：:A sensitive detection system specific for Legionella pneumophila in water was developed. This system is based on amplification of a chromosomal DNA sequence from L. pneumophila by the polymerase chain reaction, followed by detection of the amplified product by hybridization of a radiolabeled oligodeoxynucleotide. Afte...

journal_title：Journal of clinical microbiology

authors： Starnbach MN,Falkow S,Tompkins LS

更新日期：1989-06-01 00:00:00

abstract：:Several studies have identified nonsterile blood collection tubes as the reservoir for contaminating microorganisms in outbreaks of pseudobacteremia. Microorganisms are thought to be transferred from the nonsterile tubes to the blood culture tubes when blood is drawn for cultures, but the validity of this hypothesis h...

journal_title：Journal of clinical microbiology

authors： McNeil MM,Davis BJ,Anderson RL,Martone WJ,Solomon SL

更新日期：1985-07-01 00:00:00

abstract：:The development of a rapid test to identify Mycobacterium tuberculosis Beijing isolates and specifically strain GC1237, coming from a sub-Saharan country, is needed due to its alarming wide spread on Gran Canaria Island (Spain). A rapid test that detects IS6110 present between dnaA and dnaN in the Beijing strains and ...

journal_title：Journal of clinical microbiology

authors： Millán-Lou MI,Alonso H,Gavín P,Hernández-Febles M,Campos-Herrero MI,Copado R,Cañas F,Kremer K,Caminero JA,Martín C,Samper S

更新日期：2012-02-01 00:00:00

abstract：:Sputum specimens received for the diagnosis of tuberculosis or other mycobacterial infections were tested by a ligase chain reaction (LCR)-based assay and acid-fast stain and culture techniques. Results from the LCR assay (Abbott LCx Mycobacterium tuberculosis [MTB] Assay) were compared to results from standard cultur...

journal_title：Journal of clinical microbiology

authors： Moore DF,Curry JI

更新日期：1998-04-01 00:00:00

abstract：:INNO-LiPA and PapType human papillomavirus (HPV) genotyping assays were compared for detection of HPV genotypes on archival vulvar tissue. The INNO-LiPA assay detected 49 HPV-16 infections, compared with 47 detected by the PapType assay. The INNO-LiPA assay detected amplifiable DNA in 59 (91%) biopsy specimens, compar...

journal_title：Journal of clinical microbiology

authors： Tan SE,Garland SM,Rumbold AR,Tabrizi SN

更新日期：2011-11-01 00:00:00