Detection and identification of Mycobacterium tuberculosis directly from sputum sediments by ligase chain reaction.

abstract：:We describe a 2009 H1N1 virus infection with a high viral load in a previously healthy infant who presented with complex febrile seizures and improved on oseltamivir without neurologic sequelae. Febrile seizures may be a complication in young children experiencing infection with high viral loads of 2009 H1N1 influenza...

journal_title：Journal of clinical microbiology

authors： O'Leary MF,Chappell JD,Stratton CW,Cronin RM,Taylor MB,Tang YW

更新日期：2010-10-01 00:00:00

abstract：:Detection of human cytomegalovirus (CMV) DNA in clinical specimens is considered a cornerstone in the diagnosis of CMV disease. The aim of this study was to evaluate a newly designed LightCycler-based quantitative CMV PCR. Specimens of human origin (n = 200) were tested using the LightCycler PCR, the quantitative COBA...

journal_title：Journal of clinical microbiology

authors： Schaade L,Kockelkorn P,Ritter K,Kleines M

更新日期：2000-11-01 00:00:00

abstract：:A total of 242 isolates were recovered from 76 patients with invasive diseases, 89 with scarlet fever, and 77 with pharyngitis. The most frequent emm types were types 12 (43.4%), 4 (18.2%), and 1 (16.9%). emm12 reemerged in 2005 and peaked in 2007. emm11 was recovered only from patients with invasive disease. ...

journal_title：Journal of clinical microbiology

authors： Su YF,Wang SM,Lin YL,Chuang WJ,Lin YS,Wu JJ,Lin MT,Liu CC

更新日期：2009-08-01 00:00:00

abstract：:Twenty-four isolates of Actinobacillus seminis were typed by PCR ribotyping, repetitive extragenic palindromic element (REP)-based PCR, and enterobacterial repetitive intergenic consensus (ERIC)-based PCR. Five types were distinguished by REP-PCR, and nine types were distinguished by ERIC-PCR. PCR ribotyping produced ...

journal_title：Journal of clinical microbiology

authors： Appuhamy S,Coote JG,Low JC,Parton R

更新日期：1998-03-01 00:00:00

abstract：:Methodologies for biochemical identification of mycobacteria isolated from clinical samples are still cumbersome, taking skilled technicians 3 to 6 weeks. We describe here a 2-h identification system for mycobacterial isolates belonging to the Mycobacterium tuberculosis complex using a DNA probe. After 30 min of hands...

journal_title：Journal of clinical microbiology

authors： Mabilat C,Desvarenne S,Panteix G,Machabert N,Bernillon MH,Guardiola G,Cros P

更新日期：1994-11-01 00:00:00

abstract：:A comparative study of herpes simplex virus diagnosis by standard cell culture and a new hybrid test (enzyme-linked immunosorbent assay spin amplification technique) was done on 300 specimens. The new test was found to be equally sensitive and specific, much less expensive to perform, and to report all results in 48 h...

journal_title：Journal of clinical microbiology

authors： Michalski FJ,Shaikh M,Sahraie F,Desai S,Verano L,Vallabhaneni J

更新日期：1986-08-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay method was used to classify bovine serum as positive, negative, or doubtful for antibodies to Brucella abortus. Spectrophotometric data from assays of 64 serologically positive and 32 serologically negative bovine sera were analyzed statistically to define the range of spectrophoto...

journal_title：Journal of clinical microbiology

authors： Heck FC,Williams JD,Pruett J

更新日期：1980-04-01 00:00:00

abstract：:Typing of Mycobacterium avium subspecies paratuberculosis strains presents a challenge, since they are genetically monomorphic and traditional molecular techniques have limited discriminatory power. The recent advances and availability of whole-genome sequencing have extended possibilities for the characterization of ...

journal_title：Journal of clinical microbiology

authors： Leão C,Goldstone RJ,Bryant J,McLuckie J,Inácio J,Smith DG,Stevenson K

更新日期：2016-03-01 00:00:00

abstract：:Infections due to small-colony variants (SCVs) of Staphylococcus aureus in patients with chronic and recurrent infections are an emerging problem; however, studies with this subpopulation are hampered by the fact that SCVs may exhibit unstable phenotypes, making them difficult to study, particularly in broth media. In...

journal_title：Journal of clinical microbiology

authors： Becker K,Laham NA,Fegeler W,Proctor RA,Peters G,von Eiff C

更新日期：2006-09-01 00:00:00

abstract：:The highly specific and sensitive latex agglutination test for cryptococcal antigen detection in cerebrospinal fluid is routine in many hospitals. Contamination of cerebrospinal fluid by a minute amount of syneresis fluid (surface condensation) from agar gave a strongly positive reaction which was heat stable, was not...

journal_title：Journal of clinical microbiology

authors： Boom WH,Piper DJ,Ruoff KL,Ferraro MJ

更新日期：1985-11-01 00:00:00

abstract：:The identification of pathogens in patients with bacterial keratitis remains problematic because standard diagnostic tests are negative for 40 to 60% of patients. A cross-sectional study was undertaken to determine if PCR and sequence analysis of 16S ribosomal DNA (rDNA) could be used to detect bacterial pathogens in ...

journal_title：Journal of clinical microbiology

authors： Knox CM,Cevellos V,Dean D

更新日期：1998-12-01 00:00:00

abstract：:Microagglutination and micro-enzyme-linked immunosorbent assay (ELISA) tests with easily prepared, safe, heat-killed antigens for detecting antibodies to the legionnaires disease organism have been developed. A safranin-stained whole-cell antigen is used in the microagglutination test, and a simply prepared soluble an...

journal_title：Journal of clinical microbiology

authors： Farshy CE,Klein GC,Feeley JC

更新日期：1978-04-01 00:00:00

abstract：:We have evaluated the diagnostic utility of eleven Toxoplasma gondii recombinant antigens (P22 [SAG2], P24 [GRA1], P25, P28 [GRA2], P29 [GRA7], P30 [SAG1], P35, P41 [GRA4], P54 [ROP2], P66 [ROP1], and P68) in immunoglobulin G (IgG) and IgM recombinant enzyme-linked immunosorbent assays (Rec-ELISAs). Following an initi...

journal_title：Journal of clinical microbiology

authors： Aubert D,Maine GT,Villena I,Hunt JC,Howard L,Sheu M,Brojanac S,Chovan LE,Nowlan SF,Pinon JM

更新日期：2000-03-01 00:00:00

abstract：:A method for the rapid detection of verotoxin-producing Escherichia coli in stool samples by PCR was evaluated. Verotoxin-1 and verotoxin-2 genes in DNA extracted directly from stool samples were amplified with oligonucleotide primers. Stools spiked with control organisms, E. coli C600 (H19B) (verotoxin-1) or E. coli ...

journal_title：Journal of clinical microbiology

authors： Ramotar K,Waldhart B,Church D,Szumski R,Louie TJ

更新日期：1995-03-01 00:00:00

abstract：:We isolated Shiga toxin-producing Escherichia coli O157:H7 strains resistant to third-generation cephalosporins. The resistant strains harbored blaCMY-2, a plasmid-mediated AmpC β-lactamase. Genotyping of isolates revealed the possible spread of this problematic bacterium. Results suggested the importance of the inves...

journal_title：Journal of clinical microbiology

authors： Kawahara R,Seto K,Taguchi M,Nakajima C,Kumeda Y,Suzuki Y

更新日期：2015-09-01 00:00:00

abstract：:Group B streptococci (GBS) cause severe invasive disease in both neonates and adults. Understanding the epidemiology of GBS provides information that can include determining disease prevalence rates in defined populations and geographic regions, documenting the success of GBS screening programs, and understanding anti...

journal_title：Journal of clinical microbiology

authors： Alhhazmi A,Hurteau D,Tyrrell GJ

更新日期：2016-07-01 00:00:00

abstract：:mariPOC is a novel point-of-care test system for rapid detection of respiratory tract infections. We compared the performance of the mariPOC test to that of bacterial culture for detecting group A streptococcus (GAS) in 219 pharyngitis patients (ages 1-64 years) and 109 healthy asymptomatic controls (ages 19-69 years)...

journal_title：Journal of clinical microbiology

authors： Vakkila J,Koskinen JO,Brandt A,Muotiala A,Liukko V,Soittu S,Meriluoto S,Vesalainen M,Huovinen P,Irjala K

更新日期：2015-07-01 00:00:00

abstract：:The staphylococcal methicillin resistance determinant, mecA, resides on a mobile genetic element, staphylococcus chromosomal cassette mec (SCCmec). The distribution of SCCmec in nature is limited to relatively few clonal complexes of related methicillin-resistant Staphylococcus aureus (MRSA). We have previously report...

journal_title：Journal of clinical microbiology

authors： Katayama Y,Robinson DA,Enright MC,Chambers HF

更新日期：2005-05-01 00:00:00

abstract：:Buruli ulcer, an infection caused by Mycobacterium ulcerans, is, after tuberculosis and leprosy, the third most common mycobacterial disease. The mode of transmission of M. ulcerans is not exactly known, but since Buruli ulcer often occurs in focalized swampy areas, it is assumed that there is a reservoir of the patho...

journal_title：Journal of clinical microbiology

authors： Rondini S,Mensah-Quainoo E,Troll H,Bodmer T,Pluschke G

更新日期：2003-09-01 00:00:00

abstract：:Direct inoculation onto Granada medium (GM) in plates and tubes was compared to inoculation into a selective Todd-Hewitt broth (with 8 microg of gentamicin per ml and 15 microg of nalidixic acid per ml) for detection of group B streptococci (GBS) in pregnant women with 800 vaginal and 450 vaginoanorectal samples. Comp...

journal_title：Journal of clinical microbiology

authors： Rosa-Fraile M,Rodriguez-Granger J,Cueto-Lopez M,Sampedro A,Gaye EB,Haro JM,Andreu A

更新日期：1999-08-01 00:00:00

abstract：:The ERA strain of rabies virus was adapted to growth on monolayers of a porcine kidney cell line (PK-2A). A reproducible plaque assay system was subsequently developed, which appears to be satisfactory for conducting plaque neutralization tests. ...

journal_title：Journal of clinical microbiology

authors： Strating A,Mitzel JR,Slater EA

更新日期：1976-08-01 00:00:00

abstract：:Selenomonas sputigena is a curved motile anaerobic gram-negative rod that is a part of the normal upper respiratory tract of man. We present a case of speticemia associated with this organism and believe it to be the first such report describing systemic disease. ...

journal_title：Journal of clinical microbiology

authors： McCarthy LR,Carlson JR

更新日期：1981-12-01 00:00:00

abstract：:A clinical evaluation of the Amplicor polymerase chain reaction (PCR) assay for the detection of Chlamydia trachomatis in endocervical swabs (Roche Molecular Systems, Branchburg, N.J.) is described. This new clinical system used one-step sample preparation, amplification with biotinylated cryptic plasmid primer pairs ...

journal_title：Journal of clinical microbiology

authors： Bass CA,Jungkind DL,Silverman NS,Bondi JM

更新日期：1993-10-01 00:00:00

abstract：:Recently, three novel avian-origin swine influenza viruses (SIVs) were first isolated from pigs in Guangdong Province, southern China, yet little is known about the seroprevalence of avian influenza viruses among pigs in southern China. Here, we report for the first time the seroprevalence of avian H3, H4, and H6 infl...

journal_title：Journal of clinical microbiology

authors： Su S,Qi W,Chen J,Zhu W,Huang Z,Xie J,Zhang G

更新日期：2013-02-01 00:00:00

abstract：:We have shown that a modification of the color-coded antigen developed by Kasatiya and Lambert (Appl. Microbiol. 28:317-318, 1974) can be used as a substitute for other nontreponemal antigens used in screening tests for syphilis. The antigen is based on the Venereal Disease Research Laboratory antigen, with EDTA, chol...

journal_title：Journal of clinical microbiology

authors： Pettit DE,Larsen SA,Harbec PS,Feeley JC,Parham CE,Cruce DD,Hambie EA,Perryman MW

更新日期：1983-11-01 00:00:00

abstract：:This study determined the group A rotavirus occurrence in pig farms from 7 different cities in São Paulo State, Brazil. Out of 143 samples, 70 tested positive. Sequence analyses of 37 strains indicated that the strains had the G3, G5, G9, and P[6], P[13]/P[22]-like, and P[23] genotypes. ...

journal_title：Journal of clinical microbiology

authors： Tonietti PO,Hora AS,Silva FD,Ruiz VL,Gregori F

更新日期：2013-08-01 00:00:00

abstract：:Salmonella enteritidis, serotype Horsham, identified rarely worldwide, was isolated from the stools of six American Indians with gastroenteritis. The patients were from separate tribes located in three geographically distinct villages. Although significant contact was demonstrated between ill individuals within two tr...

journal_title：Journal of clinical microbiology

authors： Castle SP,Nims LJ,Lapham SC

更新日期：1983-07-01 00:00:00

abstract：:We evaluated two commercial F1 antigen capture-based immunochromatographic dipsticks, Yersinia Pestis (F1) Smart II and Plague BioThreat Alert test strips, in detecting plague bacilli by using whole-blood samples from mice experimentally infected with Yersinia pestis CO92. To assess the specificities of these dipstick...

journal_title：Journal of clinical microbiology

authors： Sha J,Endsley JJ,Kirtley ML,Foltz SM,Huante MB,Erova TE,Kozlova EV,Popov VL,Yeager LA,Zudina IV,Motin VL,Peterson JW,DeBord KL,Chopra AK

更新日期：2011-05-01 00:00:00

abstract：:Fifteen monoclonal antibodies (MAbs) made to coxsackievirus B-3 were tested against a panel of enteroviruses by indirect immunofluorescence. The MAbs included seven which reacted with coxsackievirus B-3 only, five which reacted with more than one enterovirus included in the panel, one which reacted with broad enterovi...

journal_title：Journal of clinical microbiology

authors： Yagi S,Schnurr D,Lin J

更新日期：1992-09-01 00:00:00

abstract：:The hexagonal surface structure believed to be characteristic for influenza C virus was not initially observed for a newly isolated virus of this type. After a few additional egg passages of the virus, however, the hexagonal structure could be readily seen on many virions. ...

journal_title：Journal of clinical microbiology

authors： Martin ML,Palmer EL,Kendal AP

更新日期：1977-07-01 00:00:00