Phylogenetic analyses of the VP4 and VP7 genes of porcine group A rotaviruses in Sao Paulo State, Brazil: first identification of G5P[23] in piglets.

abstract：:Stool specimens from 124 international travelers with acute diarrhea were tested for the presence of enteropathogens. Noroviruses (NoVs) were the second most commonly identified enteric pathogen in diarrheal stool samples (21/124, 17%), exceeded only by enterotoxigenic Escherichia coli (50/106, 47%). This study indica...

journal_title：Journal of clinical microbiology

authors： Ko G,Garcia C,Jiang ZD,Okhuysen PC,Belkind-Gerson J,Glass RI,DuPont HL

更新日期：2005-12-01 00:00:00

abstract：:We report a case where a mite egg found in urine caused diagnostic confusion. The possibility of gut or bladder mite infection should be entertained only after repeated identification of mites in urine or stool samples from a symptomatic patient with no other cause for the symptoms and where the possibilities of conta...

journal_title：Journal of clinical microbiology

authors： Dini LA,Frean JA

更新日期：2005-12-01 00:00:00

abstract：:Simple enzymatic assays to detect heat-labile enterotoxins whose modes of action are similar to that of cholera toxin were evaluated. The assays are performed by using an artificial substrate, diethylamino benzylidine-aminoguanidine, which is an ADP-ribose acceptor. The product, formed in the presence of NAD+, can be ...

journal_title：Journal of clinical microbiology

authors： Narayanan J,Hartman PA,Graves DJ

更新日期：1989-11-01 00:00:00

abstract：:Antisera prepared against the eight recognized serotypes of Ureaplasma urealyticum were tested against the homologous and heterologous antigens by a modified metabolic inhibition test that used bromothymol blue broth in microtiter plates. The method gives clear endpoint determinations which are usually maintained on c...

journal_title：Journal of clinical microbiology

authors： Robertson JA,Stemke GW

更新日期：1979-06-01 00:00:00

abstract：:The emergence of extended-spectrum beta-lactamase (ESBL) and plasmid-mediated AmpC (pAmpC) enzymes in Escherichia coli raises concern regarding accurate laboratory detection and interpretation of susceptibility testing results. Twenty-six cefpodoxime ESBL screen-positive, cefoxitin-resistant E. coli clinical isolates ...

journal_title：Journal of clinical microbiology

authors： Robberts FJ,Kohner PC,Patel R

更新日期：2009-02-01 00:00:00

abstract：:The comparison of Gram-stained urethral smears with Gen-Probe for the detection of Neisseria Gonorrhoeae in the urethras of males with symptomatic urethritis revealed a 99.6% correlation between the two methods. A simple Gram stain would appear to be the method of choice for the detection of gonorrhea in symptomatic m...

journal_title：Journal of clinical microbiology

authors： Juchau SV,Nackman R,Ruppart D

更新日期：1995-11-01 00:00:00

abstract：:Because of their biofilm-forming capacity, invasive Staphylococcus epidermidis isolates, which cause the majority of nosocomial catheter-related bloodstream infections (BSIs), are thought to be selected at the time of catheter insertion from a population of less virulent commensal strains. This fact allows the predict...

journal_title：Journal of clinical microbiology

authors： Rohde H,Kalitzky M,Kröger N,Scherpe S,Horstkotte MA,Knobloch JK,Zander AR,Mack D

更新日期：2004-12-01 00:00:00

abstract：:Sera from patients with systemic infections caused by the opportunistic fungus Trichosporon beigelii have been shown to cross-react with anticryptococcal antibodies. We quantitatively compared the amounts of antigen produced and examined the expression of O-acetyl epitopes from 35 strains of T. beigelii isolated from ...

journal_title：Journal of clinical microbiology

authors： Lyman CA,Devi SJ,Nathanson J,Frasch CE,Pizzo PA,Walsh TJ

更新日期：1995-01-01 00:00:00

abstract：:First-catch early morning urine samples from patients attending a genitourinary medicine clinic were tested by three different enzyme immunoassays. The results suggest significant differences in the sensitivities and specificities of the different assays. The direct visualization of elementary bodies in urine deposits...

journal_title：Journal of clinical microbiology

authors： Paul ID,Caul EO

更新日期：1990-02-01 00:00:00

abstract：:The factors that regulate cytomegalovirus (CMV) excretion from the genitourinary tract are poorly understood. To assess the role of cell-mediated immunity in such excretion, a CMV-specific mononuclear blastogenesis assay was used to study a predominantly lower-socioeconomic-status population of 92 healthy nonpregnant ...

journal_title：Journal of clinical microbiology

authors： Faix RG,Zweig SE,Kummer JF,Moore D,Lang DJ

更新日期：1983-04-01 00:00:00

abstract：:The Vitek MS v2.0 matrix-assisted laser desorption ionization-time of flight mass spectrometry system accurately distinguished Streptococcus pneumoniae from nonpneumococcal S. mitis group species. Only 1 of 116 nonpneumococcal isolates (<1%) was misidentified as S. pneumoniae. None of 95 pneumococcal isolates was misi...

journal_title：Journal of clinical microbiology

authors： Branda JA,Markham RP,Garner CD,Rychert JA,Ferraro MJ

更新日期：2013-09-01 00:00:00

abstract：:In a multicenter study, the consistency of international units expressed by five commercially available rubella virus immunoglobulin G kits was evaluated. The linearity and within-run and between-run precision were determined for each kit. All kits demonstrated good linearity and had within-run and between-run precisi...

journal_title：Journal of clinical microbiology

authors： Dimech W,Bettoli A,Eckert D,Francis B,Hamblin J,Kerr T,Ryan C,Skurrie I

更新日期：1992-03-01 00:00:00

abstract：:Sera (920) were tested to evaluate the use of heated versus unheated sera in the hemagglutination treponemal test for syphilis. The heated and unheated samples were tested on the same day with the hemagglutination treponemal test for syphilis kit according to the manufacturer's protocol. Agreement of results between t...

journal_title：Journal of clinical microbiology

authors： Hambie EA,Larsen SA,Perryman MW,Pettit DE,Feeley JC

更新日期：1982-02-01 00:00:00

abstract：:Various fixatives and treatments such as acetone, methanol, Bouin fixative, modified Bouin fixative, 10% Formalin, modified methacarn, periodate-lysine-paraformaldehyde, acetone-methyl benzoate-xylene, and EDTA were evaluated for their effect on the immunoreactivity of Coxiella burnetii in paraffin-embedded tissues by...

journal_title：Journal of clinical microbiology

authors： Baumgärtner W,Dettinger H,Schmeer N,Hoffmeister E

更新日期：1988-10-01 00:00:00

abstract：:The kinetics of delta antigen (HDAg) and anti-delta antibody (anti-HD) was analyzed in 22 acute delta hepatitis infections (11 coinfections and 11 superinfections), with an enzyme immunoassay developed by Organon Teknika and with two commercially available assays: Deltassay, a test for HDAg from Noctech and Abbott ant...

journal_title：Journal of clinical microbiology

authors： Dubois F,Goudeau A

更新日期：1988-07-01 00:00:00

abstract：:A novel recombinant baculovirus which expresses Ebola virus (EBO) nucleoprotein (NP) under the control of the cytomegalovirus immediate-early promoter was constructed. HeLa cells abortively infected with the baculovirus expressed EBO NP, and this was used as an immunofluorescent (IF) antigen to detect EBO immunoglobul...

journal_title：Journal of clinical microbiology

authors： Saijo M,Niikura M,Morikawa S,Kurane I

更新日期：2001-02-01 00:00:00

abstract：:A simple, relatively rapid silver impregnation stain has been found to stain Legionella pneumophila effectively in paraffin-embedded tissue sections while permitting visualization of histological detail. It may also be used to stain the organism in body fluids. The stain is not specific and thus must be confirmed by d...

journal_title：Journal of clinical microbiology

authors： Faine S,Edelstein P,Kirby BD,Finegold SM

更新日期：1979-07-01 00:00:00

abstract：:Metarhizium viride has been associated with fatal systemic mycoses in chameleons, but subsequent data on mycoses caused by this fungus in reptiles are lacking. The aim of this investigation was therefore to obtain information on the presence of M. viride in reptiles kept as pets in captivity and its association with c...

journal_title：Journal of clinical microbiology

authors： Schmidt V,Klasen L,Schneider J,Hübel J,Pees M

更新日期：2017-03-01 00:00:00

abstract：:Conserved primers were used to PCR amplify 95% of the Helicobacter hepaticus 16S rRNA gene. Its sequence was determined and aligned to those of related bacteria, enabling the selection of primers to highly diverged regions of the 16S rRNA gene and an oligonucleotide probe for the development of a PCR-liquid hybridizat...

journal_title：Journal of clinical microbiology

authors： Battles JK,Williamson JC,Pike KM,Gorelick PL,Ward JM,Gonda MA

更新日期：1995-05-01 00:00:00

abstract：:The value of performing routine aerobic subcultures of both vented and unvented blood culture bottles has not been evaluated critically. We studied 4,954 pairs of blood culture bottles consisting of one vented biphasic tryptic soy broth bottle (Roche Diagnostics) and one unvented Thiol broth bottle (Difco Laboratories...

journal_title：Journal of clinical microbiology

authors： Pfaller MA,Westfall LM,Murray PR

更新日期：1983-04-01 00:00:00

abstract：:This three-center collaborative study was conducted to evaluate samples of the Microbial Profile System (MPS) antimicrobial microdilution panels [previously produced by Minnesota Mining & Manufacturing Co., (3M Co.), St. Paul, Minn. and currently produced by Flow Laboratories, Inc., Rockville, Md.). This was a three-p...

journal_title：Journal of clinical microbiology

authors： Gerlach EH,Jones RN,Barry AL

更新日期：1983-03-01 00:00:00

abstract：:A new anti-human immunodeficiency virus type 1 and 2 (anti-HIV 1 and 2) test is described. It uses recombinant p24 and peptides covering gp32, gp41, and gp120 to identify HIV-1 and HIV-2 infections. This test has been shown to be specific (99.5%) and sensitive (99.8%). In this respect, the assay was equal or superior ...

journal_title：Journal of clinical microbiology

authors： Hess G,Avillez F,Lourenco MH,D'Agostino F,Cambie G,Piot P,Vercauteren G,Michl U,Melchior W,Bayer H

更新日期：1994-02-01 00:00:00

abstract：:Misidentification of coagulase-negative staphylococci (CoNS) may delay appropriate treatment. We investigated 20 clinical isolates identified as Kocuria spp. by the Vitek 2 system. All were identified as CoNS by 16S rRNA gene sequencing (18 Staphylococcus epidermidis, 1 Staphylococcus haemolyticus). Four Kocuria isola...

journal_title：Journal of clinical microbiology

authors： Ben-Ami R,Navon-Venezia S,Schwartz D,Schlezinger Y,Mekuzas Y,Carmeli Y

更新日期：2005-03-01 00:00:00

abstract：:Four human coronaviruses (HCoV-229E, HCoV-HKU1, HCoV-NL63, and HCoV-OC43) are associated with a range of respiratory outcomes, including bronchiolitis and pneumonia. Their epidemiologies and clinical characteristics are poorly described and are often reliant on case reports. To address these problems, we conducted a l...

journal_title：Journal of clinical microbiology

authors： Gaunt ER,Hardie A,Claas EC,Simmonds P,Templeton KE

更新日期：2010-08-01 00:00:00

abstract：:A group of 254 isolates of coagulase-negative staphylococci were tested in parallel for novobiocin susceptibility by using P agar and Mueller-Hinton agar. Zones of inhibition of 16 mm or less around a 5-micrograms novobiocin disk on Mueller-Hinton agar indicated novobiocin resistance, as demonstrated by Staphylococcus...

journal_title：Journal of clinical microbiology

authors： Almeida RJ,Jorgensen JH

更新日期：1982-12-01 00:00:00

abstract：:The effects of Tween 80 supplementation of liquid culture medium on the formation of the superficial L1 layer of the Mycobacterium avium-Mycobacterium intracellulare complex (MAC) were examined by serological and scanning electron microscopic experiments. Specific antiserum to the glycopeptidolipids on the L1 layer of...

journal_title：Journal of clinical microbiology

authors： Masaki S,Sugimori G,Okamoto A,Imose J,Hayashi Y

更新日期：1991-07-01 00:00:00

abstract：:A ligation-mediated PCR (LMPCR) method for the amplification of sequences flanking the IS6110 of the Mycobacterium tuberculosis complex has been developed. The method uses one primer specific for IS6110 and a second specific for a linker ligated to SalI-restricted genomic DNA. LMPCR is a rapid screening method, valuab...

journal_title：Journal of clinical microbiology

authors： Prod'hom G,Guilhot C,Gutierrez MC,Varnerot A,Gicquel B,Vincent V

更新日期：1997-12-01 00:00:00

abstract：:Many central vascular catheters (CVCs) are removed unnecessarily because current diagnostic methods for CVC-associated infection are unreliable. A quantitative PCR assay using primers and probe targeted to bacterial 16S ribosomal DNA was used to measure the levels of bacterial DNA in blood samples drawn through the CV...

journal_title：Journal of clinical microbiology

authors： Warwick S,Wilks M,Hennessy E,Powell-Tuck J,Small M,Sharp J,Millar MR

更新日期：2004-04-01 00:00:00

abstract：:Tissue cultures established from brain tissues of six patients with various chronic, degenerative diseases did not show evidence of viral agents. Sucrose gradient analysis of radioactively labeled culture fluid from two patients failed to reveal the presence of any incomplete or defective virus. Extracellular fluids f...

journal_title：Journal of clinical microbiology

authors： Lennette ET

更新日期：1975-05-01 00:00:00

abstract：:In a stress test, the recently introduced RapID CB Plus system (Remel Inc. [formerly Innovative Diagnostic Systems], Norcross, Ga.) was challenged with a diverse set of gram-positive rods comprising 345 strains of coryneform bacteria and 33 strains of Listeria spp. representing a total of 49 different taxa. Overall, w...

journal_title：Journal of clinical microbiology

authors： Funke G,Peters K,Aravena-Roman M

更新日期：1998-09-01 00:00:00