Diagnosis of human immunodeficiency virus (HIV) infection: multicenter evaluation of a newly developed anti-HIV 1 and 2 enzyme immunoassay.

abstract：:Lipopolysaccharide components 3-deoxy-D-manno-2-octulosonic acid and L-glycero-D-manno-heptose were detected in hydrolysates from whole cells of Neisseria elongata by gas-liquid chromatography. Cells from a single plate were hydrolyzed, and carbohydrate components were converted to aldononitrile and O-methyloxime acet...

journal_title：Journal of clinical microbiology

authors： Guerrant GO,Moss CW

更新日期：1986-03-01 00:00:00

abstract：:A newly developed commercial serological test (Iatron Laboratories, Inc., Tokyo, Japan) for the rapid identification of medically important species of Candida was evaluated against the API 20C (Analytab Products, Plainview, N.Y.) and the standard Wickerham assimilation and fermentation procedures. Our results indicate...

journal_title：Journal of clinical microbiology

authors： Shinoda T,Kaufman L,Padhye AA

更新日期：1981-03-01 00:00:00

abstract：:The new colorimetric Vitek 2 YST card was evaluated for identification of yeasts (136 strains) with respect to the influence of different source media. The Vitek 2 YST card achieved satisfactory results for all yeast species tested, with the exception of Candida guilliermondii, Candida norvegensis, Candida parapsilosi...

journal_title：Journal of clinical microbiology

authors： Valenza G,Strasen J,Schäfer F,Frosch M,Kurzai O,Abele-Horn M

更新日期：2008-11-01 00:00:00

abstract：:Rotaviruses were detected by counterimmunoelectro-osmophoresis in the feces of 16 (13.8%) of 116 infants and young children with gastroenteritis during a 5-month period (September 1979 through January 1980) in Ife, Nigeria. The rate of rotavirus detection varied inversely with relative humidity and was highest in Dece...

journal_title：Journal of clinical microbiology

authors： Paul MO,Erinle EA

更新日期：1982-02-01 00:00:00

abstract：:In a collaborative study at three university hospitals, the recovery of microorganisms and the speed of detection of microbial growth by the BacT/Alert (Organon Teknika Corporation, Durham, N.C.) and BACTEC 660/730 (Becton-Dickinson Diagnostic Instrument Systems, Sparks, Md.) nonradiometric blood culture systems were ...

journal_title：Journal of clinical microbiology

authors： Wilson ML,Weinstein MP,Reimer LG,Mirrett S,Reller LB

更新日期：1992-02-01 00:00:00

abstract：:The genetic compositions of 71 isoniazid-resistant Mycobacterium tuberculosis strains from Poland were determined by spoligotyping. Nearly 80% of the isolates belonged to either the T or the Haarlem family. The genotypic diversity was largely due to variation within those families. The scarcity of imported genotypes s...

journal_title：Journal of clinical microbiology

authors： Augustynowicz-Kopec E,Jagielski T,Zwolska Z

更新日期：2008-12-01 00:00:00

abstract：:The role of atypical enteropathogenic Escherichia coli (EPEC) in childhood diarrhea is controversial. The aim of the present study was to search for genes linked with diarrhea in atypical EPEC strains from a case-control study among Norwegian children. Using DNA microarray analysis, genomic DNAs from strains isolated ...

journal_title：Journal of clinical microbiology

authors： Afset JE,Bruant G,Brousseau R,Harel J,Anderssen E,Bevanger L,Bergh K

更新日期：2006-10-01 00:00:00

abstract：:The Elecsys Anti-SARS-CoV-2 immunoassay (Roche Diagnostics) was developed to provide accurate, reliable detection of antibodies to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We evaluated sensitivity, specificity, cross-reactivity, and agreement with a vesicular stomatitis virus-based pseudoneutraliz...

journal_title：Journal of clinical microbiology

authors： Muench P,Jochum S,Wenderoth V,Ofenloch-Haehnle B,Hombach M,Strobl M,Sadlowski H,Sachse C,Torriani G,Eckerle I,Riedel A

更新日期：2020-09-22 00:00:00

abstract：:In this study we describe a multiplex PCR assay for the detection of nine clinically relevant antibiotic resistance genes of Staphylococcus aureus. Conditions were optimized to amplify fragments of mecA (encoding methicillin resistance), aacA-aphD (aminoglycoside resistance), tetK, tetM (tetracycline resistance), erm(...

journal_title：Journal of clinical microbiology

authors： Strommenger B,Kettlitz C,Werner G,Witte W

更新日期：2003-09-01 00:00:00

abstract：:Clostridium difficile is the leading cause of antibiotic-associated diarrhea and pseudomembranous colitis, which have significant morbidity and mortality. Accurate and timely diagnosis is critical. Repeat enzyme immunoassay testing for C. difficile toxin has been recommended because of <100% sensitivity. All C. diffic...

journal_title：Journal of clinical microbiology

authors： Cardona DM,Rand KH

更新日期：2008-11-01 00:00:00

abstract：:We determined the quantitative levels and the genetic mechanisms of resistance in drug-resistant clinical isolates of Mycobacterium tuberculosis sampled over a period of 3 years (n = 45; 17 of the isolate were multidrug resistant). Our results led us to hypothesize that some strains categorized as resistant to isoniaz...

journal_title：Journal of clinical microbiology

authors： Springer B,Calligaris-Maibach RC,Ritter C,Böttger EC

更新日期：2008-12-01 00:00:00

abstract：:Forty-eight clinical Acinetobacter isolates with different epidemic behavior were investigated for the presence of integrons and plasmids and for antibiotic susceptibility. Integrons were demonstrated in 50% of the strains by an integrase gene PCR. Epidemic strains of Acinetobacter baumannii were found to contain sign...

journal_title：Journal of clinical microbiology

authors： Koeleman JG,Stoof J,Van Der Bijl MW,Vandenbroucke-Grauls CM,Savelkoul PH

更新日期：2001-01-01 00:00:00

abstract：:A reverse transcriptase PCR (RT-PCR) was used for rapid determination of the hemagglutinin (HA) cleavage site sequence, a marker for the virulence potential of avian influenza viruses. When applied to specimens from chickens experimentally infected with either a virulent or an avirulent virus, RT-PCR uniformly detecte...

journal_title：Journal of clinical microbiology

authors： Horimoto T,Kawaoka Y

更新日期：1995-03-01 00:00:00

abstract：:This study used a nested multiplex PCR method to detect three periodontal pathogens in subgingival plaque collected before treatment and at 2 and 6 months posttreatment from 107 patients with severe, generalized periodontitis. The proportions of the patients who harbored these bacteria before periodontal treatment wer...

journal_title：Journal of clinical microbiology

authors： Ready D,D'Aiuto F,Spratt DA,Suvan J,Tonetti MS,Wilson M

更新日期：2008-10-01 00:00:00

abstract：:An enzymatic profile of 20 strains of Pseudomonas maltophilia was undertaken with conventional plate tests, API ZYM, and 4-methylumbelliferyl-conjugated substrates. All strains produced DNase, RNase, arbutinase, esterases and lipases, mucinase, acid and alkaline phosphatases, alkaline pyrophosphate diesterase, phospho...

journal_title：Journal of clinical microbiology

authors： O'Brien M,Davis GH

更新日期：1982-09-01 00:00:00

abstract：:Vibrio cholerae serotype O1 organisms that do not produce cholera toxin and, in fact, lack the genetic material encoding the enterotoxin have recently been detected in coastal regions of the United States. Although these organisms have been assumed to be nonpathogenic, they have been considered a potential reservoir o...

journal_title：Journal of clinical microbiology

authors： Johnston JM,McFarland LM,Bradford HB,Caraway CT

更新日期：1983-05-01 00:00:00

abstract：:Entamoeba histolytica is an intestinal parasite causing significant morbidity and mortality worldwide. More tools are needed to understand the epidemiology and molecular pathogenesis of amebiasis. A cDNA library was constructed by using poly(A)+ RNA isolated from an axenic strain of E. histolytica, HM1:IMSS, which exp...

journal_title：Journal of clinical microbiology

authors： Burch DJ,Li E,Reed S,Jackson TF,Stanley SL Jr

更新日期：1991-04-01 00:00:00

abstract：:Transfusion-transmissible infections among 808 blood donors in Ghana were investigated in 1999. Antibody seroprevalences of 3.8, 0.7, 8.4, and 13.5%, respectively, for human immunodeficiency virus, human T-cell lymphotrophic virus type 1, hepatitis C virus (HCV), and Treponema pallidum were obtained. The seroprevalenc...

journal_title：Journal of clinical microbiology

authors： Ampofo W,Nii-Trebi N,Ansah J,Abe K,Naito H,Aidoo S,Nuvor V,Brandful J,Yamamoto N,Ofori-Adjei D,Ishikawa K

更新日期：2002-09-01 00:00:00

abstract：:Neisseria meningitidis is the causative agent of meningococcal sepsis and meningitis. Neisseria polysaccharea is a nonpathogenic species. N. polysaccharea is able to use sucrose to produce amylopectin, a starch-like polysaccharide, which distinguishes it biochemically from the pathogenic species N. meningitidis. The d...

journal_title：Journal of clinical microbiology

authors： Zhu P,Tsang RS,Tsai CM

更新日期：2003-01-01 00:00:00

abstract：:Rhesus and cynomolgus monkey kidney tissue cultures and two continuous lines, Madin-Darby canine kidney (MDCK) and LLC-MK2, were compared in titrations and isolations of influenza and parainfluenza viruses. Tube cultures were inoculated with laboratory virus strains or stored patient specimens and observed for hemadso...

journal_title：Journal of clinical microbiology

authors： Frank AL,Couch RB,Griffis CA,Baxter BD

更新日期：1979-07-01 00:00:00

abstract：:The detection of amplified products resulting from polymerase chain reactions (PCRs) remains a complicated process. To simplify the detection procedures, we developed a colorimetric microtiter plate hybridization assay for the specific detection of 5'-biotinylated PCR fragments of Mycobacterium leprae DNA. For this as...

journal_title：Journal of clinical microbiology

authors： van der Vliet GM,Hermans CJ,Klatser PR

更新日期：1993-03-01 00:00:00

abstract：:During the 1971 epidemic of Venezuelan equine encephalomyelitis (VEE) in south Texas, 203 suspect VEE cases were evaluated by the Center for Disease Control. Sixty-seven were confirmed as cases of VEE. Laboratory confirmation was accomplished by isolation of VEE virus from a serum specimen taken during the acute illne...

journal_title：Journal of clinical microbiology

authors： Bowen GS,Calisher CH

更新日期：1976-07-01 00:00:00

abstract：:The 1896 precore (PC) mutation is the most frequent cause of hepatitis B virus e-antigen (HBeAg)-negative chronic hepatitis B virus (HBV) infection. Detection of the 1896 PC mutation has application in studies monitoring antiviral therapy and the natural history of the disease. Identification of this mutation is usual...

journal_title：Journal of clinical microbiology

authors： Waltz TL,Marras S,Rochford G,Nolan J,Lee E,Melegari M,Pollack H

更新日期：2005-01-01 00:00:00

abstract：:A production model of the semi-automated PASCO MIC-ID system (PASCO Laboratories, Wheat Ridge, Colo.) was evaluated with 122 groups or species of gram-negative bacilli, which included typical (499 cultures) and atypical (37 cultures) strains of fermenters and nonfermenters. The PASCO system identified 90.9% of 536 cul...

journal_title：Journal of clinical microbiology

authors： Rhoden DL,Schable B,Smith PB

更新日期：1987-12-01 00:00:00

abstract：:We report the first case of maxillary sinusitis caused by Actinomucor elegans in an 11-year-old patient. Histopathological and mycological examinations of surgical maxillary sinuses samples showed coenocytic hyphae characteristic of mucoraceous fungi. The fungi recovered had stolons and rhizoids, nonapophyseal and glo...

journal_title：Journal of clinical microbiology

authors： Davel G,Featherston P,Fernández A,Abrantes R,Canteros C,Rodero L,Sztern C,Perrotta D

更新日期：2001-02-01 00:00:00

abstract：:Antibiotic-associated hemorrhagic colitis (AAHC) is associated with Klebsiella oxytoca. This study analyzed whether cytotoxic properties are linked to specific subtypes of K. oxytoca. Klebsiella isolates from stools of AAHC patients, healthy carriers, and diarrhea patients as well as from infections of other organs we...

journal_title：Journal of clinical microbiology

authors： Joainig MM,Gorkiewicz G,Leitner E,Weberhofer P,Zollner-Schwetz I,Lippe I,Feierl G,Krause R,Hinterleitner T,Zechner EL,Högenauer C

更新日期：2010-03-01 00:00:00

abstract：:Sequential specimens from nine allograft recipients were examined by using a variety of methods to detect primary cytomegalovirus (CMV) infection as rapidly as possible posttransplantation. Sera were examined for immunoglobulin G (IgG) and IgM antibodies by immunoblotting, enzyme immunoassay, and immunofluorescence an...

journal_title：Journal of clinical microbiology

authors： Miller H,McCulloch B,Landini MP,Rossier E

更新日期：1989-12-01 00:00:00

abstract：:The incidence of vancomycin resistance among enterococci is increasing in the United States and elsewhere in the world, but automated susceptibility testing methods have difficulty detecting resistance expressed by certain strains. The agar screening method described by Willey et al. (B. M. Willey, B. N. Kreiswirth, A...

journal_title：Journal of clinical microbiology

authors： Swenson JM,Clark NC,Ferraro MJ,Sahm DF,Doern G,Pfaller MA,Reller LB,Weinstein MP,Zabransky RJ,Tenover FC

更新日期：1994-07-01 00:00:00

abstract：:The 30-micrograms cefprozil disk and the cephalosporin class (30-micrograms cephalothin) disk were compared for their abilities to predict cefprozil susceptibility by agar disk diffusion testing. High error (5.02% major and 14.11% minor) rates were encountered with the cephalothin disk and were most frequently observe...

journal_title：Journal of clinical microbiology

authors： Fung-Tomc J,Stickle T,Doyle C,Huczko E,Kessler RE

更新日期：1991-11-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay was developed for the titration of antibodies in human sera to influenza virus neuraminidase, employing partially purified N1 neuraminidase. Specificity of the test was demonstrated, and the test was more sensitive than either the conventional neuraminidase inhibition or plaque siz...

journal_title：Journal of clinical microbiology

authors： Khan MW,Gallagher M,Bucher D,Cerini CP,Kilbourne ED

更新日期：1982-07-01 00:00:00