Direct reverse transcriptase PCR to determine virulence potential of influenza A viruses in birds.

abstract：:Commercial chemiluminescent DNA probes (Accuprobe; Gen-Probe, San Diego, Calif.) for the identification of Mycobacterium tuberculosis (MTB) complex, M. avium complex (MAC), M. gordonae, and M. kansasii were evaluated with 134 clinical isolates. These included 36 MTB complex, 40 MAC, 27 M. gordonae, 9 M. kansasii, and ...

journal_title：Journal of clinical microbiology

authors： Lebrun L,Espinasse F,Poveda JD,Vincent-Levy-Frebault V

更新日期：1992-09-01 00:00:00

abstract：:Vibrio vulnificus was isolated from a bacteremic patient. This strain, together with other isolates of V. vulnificus, was compared with V. alginolyticus, V. fluvialis, and V. parahaemolyticus with regard to growth characteristics on enteric agar media (enabling isolation and identification) and production of exoenzyme...

journal_title：Journal of clinical microbiology

authors： Desmond EP,Janda JM,Adams FI,Bottone EJ

更新日期：1984-02-01 00:00:00

abstract：:Viral load testing for cytomegalovirus (CMV) has become the standard for the diagnosis of infection and monitoring of therapy at many transplant centers. However, no viral load test has been approved by the FDA. Therefore, many laboratories rely on laboratory-developed assays. This study evaluated the performance char...

journal_title：Journal of clinical microbiology

authors： Caliendo AM,Ingersoll J,Fox-Canale AM,Pargman S,Bythwood T,Hayden MK,Bremer JW,Lurain NS

更新日期：2007-06-01 00:00:00

abstract：:A method for the rapid detection of verotoxin-producing Escherichia coli in stool samples by PCR was evaluated. Verotoxin-1 and verotoxin-2 genes in DNA extracted directly from stool samples were amplified with oligonucleotide primers. Stools spiked with control organisms, E. coli C600 (H19B) (verotoxin-1) or E. coli ...

journal_title：Journal of clinical microbiology

authors： Ramotar K,Waldhart B,Church D,Szumski R,Louie TJ

更新日期：1995-03-01 00:00:00

abstract：:Alere i RSV is a novel rapid test which applies a nicking enzyme amplification reaction to detect respiratory syncytial virus in point-of-care settings. In this study, we evaluated the Alere i RSV assay by using frozen nasopharyngeal swab samples that were collected in viral transport medium from children hospitalized...

journal_title：Journal of clinical microbiology

authors： Peters RM,Schnee SV,Tabatabai J,Schnitzler P,Pfeil J

更新日期：2017-04-01 00:00:00

abstract：:The performance of a human herpesvirus 8 (HHV-8) enzyme immunoassay (EIA) and selective subsequent use of an HHV-8 immunofluorescence assay (IFA) was tested in persons unlikely to be infected with HHV-8 and those who had HHV-8 detected in their saliva. The IFA was performed on samples within a range of EIA optical den...

journal_title：Journal of clinical microbiology

authors： Casper C,Krantz E,Taylor H,Dalessio J,Carrell D,Wald A,Corey L,Ashley R

更新日期：2002-10-01 00:00:00

abstract：:We compared the Mycoplasma Duo kit (Sanofi Diagnostics Pasteur) with PCR for detection of Ureaplasma spp. in endotracheal aspirates from 60 premature neonates. The overall agreement between the two tests was 96%. The Mycoplasma Duo assay is a useful alternative to culture and PCR for detection of neonatal Ureaplasma i...

journal_title：Journal of clinical microbiology

authors： Cheah FC,Anderson TP,Darlow BA,Murdoch DR

更新日期：2005-01-01 00:00:00

abstract：:During a survey of Group G and C streptococcal infections of humans two epidemiologically unrelated Group G streptococcal isolates were identified, one from a case of bacteremia and one from a wound infection. These isolates were atypical among this sample in that the emm gene could not be amplified from them by PCR. ...

journal_title：Journal of clinical microbiology

authors： Whatmore AM,Engler KH,Gudmundsdottir G,Efstratiou A

更新日期：2001-11-01 00:00:00

abstract：:We investigated the potential of pooling DNA from nasopharyngeal specimens to reduce the cost of real-time PCR (RT-PCR) for bacterial detection. Lyophilization is required to reconcentrate DNA. This strategy yields a high specificity (86%) and a high sensitivity (96%). We estimate that compared to individual testing, ...

journal_title：Journal of clinical microbiology

authors： Edouard S,Prudent E,Gautret P,Memish ZA,Raoult D

更新日期：2015-03-01 00:00:00

abstract：:To compare Simplexa Flu A/B & RSV PCR with cytospin-immunofluorescence and laboratory-developed TaqMan PCR methods, 445 nasopharyngeal samples were tested. Of these, 199 were positive (46 for respiratory syncytial virus [RSV], 120 for influenza A, and 33 for influenza B) and 246 were negative. The direct fluorescent-a...

journal_title：Journal of clinical microbiology

authors： Landry ML,Ferguson D

更新日期：2014-08-01 00:00:00

abstract：:The commercially available diagnostic tests for Chagas' disease employ whole extracts or semipurified fractions of Trypanosoma cruzi epimastigotes. Considerable variation in the reproducibility and reliability of these tests has been reported by different research laboratories, mainly due to cross-reactivity with othe...

journal_title：Journal of clinical microbiology

authors： Umezawa ES,Bastos SF,Camargo ME,Yamauchi LM,Santos MR,Gonzalez A,Zingales B,Levin MJ,Sousa O,Rangel-Aldao R,da Silveira JF

更新日期：1999-05-01 00:00:00

abstract：:A new rapid one-step immunochromatographic test using monoclonal antibodies for detection of Helicobacter pylori antigen in stool in children was evaluated on coded stool samples from 159 children (mean age, 9.7 +/- 5.0 years; 118 from Munich, 41 from Vienna): 86 children were H. pylori infected defined by positive cu...

journal_title：Journal of clinical microbiology

authors： Antos D,Crone J,Konstantopoulos N,Koletzko S

更新日期：2005-06-01 00:00:00

abstract：:The algorithm for a new identification system was designed on the basis of colony color and morphology on CHROMagar Orientation medium in conjunction with simple biochemical tests such as indole (IND), lysine decarboxylase (LDC), and ornithine decarboxylase (ODC) utilization tests with gram-negative bacilli isolated f...

journal_title：Journal of clinical microbiology

authors： Ohkusu K

更新日期：2000-12-01 00:00:00

abstract：:We previously demonstrated that whole blood contains significantly more hepatitis C virus (HCV) RNA than plasma. To validate the whole-blood-based HCV RNA detection method, a prospective comparison of HCV RNA detection in whole blood and plasma from 50 patients with chronic liver disease was undertaken. Whole-blood an...

journal_title：Journal of clinical microbiology

authors： Stapleton JT,Klinzman D,Schmidt WN,Pfaller MA,Wu P,LaBrecque DR,Han Jq,Phillips MJ,Woolson R,Alden B

更新日期：1999-03-01 00:00:00

abstract：:Ten clinical yeast isolates submitted to the Centers for Disease Control from diverse geographic areas were identified as Candida ciferrii and Candida chiropterorum. The association of C. ciferrii with clinical specimens, particularly its repeated isolation from a case of onychomycosis, suggests that this species may ...

journal_title：Journal of clinical microbiology

authors： Furman RM,Ahearn DG

更新日期：1983-11-01 00:00:00

abstract：:Of 532 strains of viridans streptococci isolated from clinical specimens, 517 were identified by using a scheme based on the work of Facklam (R. R. Facklam, J. Clin. Microbiol. 5:184-201, 1977). The strains were distributed among nine of the species described by Facklam and an additional group of physiologically homog...

journal_title：Journal of clinical microbiology

authors： Ruoff KL,Kunz LJ

更新日期：1982-05-01 00:00:00

abstract：:We report on the performance of a recently introduced commercial chessboard method using 12 antisera, in comparison with that of the 55-antiserum panel used in determining the serogroups and types (SGTs) of Streptococcus pneumoniae, both of which were carried out by a coagglutination technique. Of a total of 150 strai...

journal_title：Journal of clinical microbiology

authors： Lalitha MK,Thomas K,Kumar RS,Steinhoff MC

更新日期：1999-01-01 00:00:00

abstract：:Vibrio cholerae serotype O1 organisms that do not produce cholera toxin and, in fact, lack the genetic material encoding the enterotoxin have recently been detected in coastal regions of the United States. Although these organisms have been assumed to be nonpathogenic, they have been considered a potential reservoir o...

journal_title：Journal of clinical microbiology

authors： Johnston JM,McFarland LM,Bradford HB,Caraway CT

更新日期：1983-05-01 00:00:00

abstract：:A new integrated extraction and real-time PCR-based system for the detection of group B streptococci in antepartum screening samples enriched in Lim broth was compared to the CDC-recommended culture method. The BD Max GBS assay exhibited acceptable sensitivity (95%) and specificity (96.7%) compared to those of the cul...

journal_title：Journal of clinical microbiology

authors： Riedlinger J,Beqaj SH,Milish MA,Young S,Smith R,Dodd M,Hankerd RE,Lebar WD,Newton DW

更新日期：2010-11-01 00:00:00

abstract：:Of a total of 1043 macrolide-resistant Streptococcus pneumoniae isolates collected from 24 countries as part of PROTEKT 1999-2000, 71 isolates tested positive for both the mef(A) and erm(B) genes. Of 69 isolates subjected to further molecular investigations, all were resistant to tetracycline, 63 (91.3%) were resistan...

journal_title：Journal of clinical microbiology

authors： Farrell DJ,Morrissey I,Bakker S,Morris L,Buckridge S,Felmingham D

更新日期：2004-02-01 00:00:00

abstract：:The cellular fatty acid composition of Legionella feeleii was determined by capillary gas-liquid chromatography, and the ubiquinone content was determined by reverse-phase high-pressure liquid chromatography. As in other Legionella species, this new species is characterized by relatively large amounts of branched-chai...

journal_title：Journal of clinical microbiology

authors： Moss CW,Bibb WF,Karr DE,Guerrant GO,Lambert MA

更新日期：1983-10-01 00:00:00

abstract：:An investigation was made on the effect of acetone fixation on infectivity and immunofluorescent antigenicity of respiratory syncytial virus and adenovirus type 5, lipid- and nonlipid-containing viruses, respectively. Viruses were allowed to replicate in HEp-2 cells, and the cells were then fixed in acetone at 5 C for...

journal_title：Journal of clinical microbiology

authors： Bardell D

更新日期：1975-02-01 00:00:00

abstract：:The description and evaluation of a PCR-based assay for the detection and species identification of the eight known human herpesviruses are presented. Two primer pairs targeting well-conserved regions of the genome allowed the amplification of the DNAs of all known human herpesviruses at a high level of sensitivity (1...

journal_title：Journal of clinical microbiology

authors： Johnson G,Nelson S,Petric M,Tellier R

更新日期：2000-09-01 00:00:00

abstract：:The new colorimetric Vitek 2 YST card was evaluated for identification of yeasts (136 strains) with respect to the influence of different source media. The Vitek 2 YST card achieved satisfactory results for all yeast species tested, with the exception of Candida guilliermondii, Candida norvegensis, Candida parapsilosi...

journal_title：Journal of clinical microbiology

authors： Valenza G,Strasen J,Schäfer F,Frosch M,Kurzai O,Abele-Horn M

更新日期：2008-11-01 00:00:00

abstract：:A methodologic study was performed to compare the polymerase chain reaction (PCR) and Southern blot hybridization, two commonly used testing strategies for the detection of human papillomavirus (HPV) infection. Three laboratories tested masked aliquots of exfoliated cervical cell specimens obtained from 120 women by c...

journal_title：Journal of clinical microbiology

authors： Schiffman MH,Bauer HM,Lorincz AT,Manos MM,Byrne JC,Glass AG,Cadell DM,Howley PM

更新日期：1991-03-01 00:00:00

abstract：:A multicenter cross-sectional survey was conducted comparing a commercially available chlamydial optical immunoassay (OIA) to the chlamydial ligase chain reaction (LCR). Endocervical samples from 415 outpatients visiting clinics from three hospitals in South Kalimantan, Indonesia, were evaluated. Relative to the LCR, ...

journal_title：Journal of clinical microbiology

authors： Widjaja S,Cohen S,Brady WE,O'reilly K,Susanto,Wibowo A,Cahyono,Graham RR,Porter KR

更新日期：1999-12-01 00:00:00

abstract：:We evaluated the Prodesse ProFlu-1 real-time reverse transcription-PCR multiplex assay with the SmartCycler instrument for the detection of human respiratory syncytial virus (RSV) and influenza A and B viruses in comparison to conventional cell culture and antigen immunoassays with the BD Directigen A+B and Binax NOW ...

journal_title：Journal of clinical microbiology

authors： Liao RS,Tomalty LL,Majury A,Zoutman DE

更新日期：2009-03-01 00:00:00

abstract：:Campylobacter species are the leading agents of bacterial gastroenteritis in developed countries. In this study 320 specimens of feces from patients with symptoms of acute gastroenteritis were cultured for Campylobacter species by direct plating on modified charcoal cefoperazone deoxycholate agar and by enrichment in ...

journal_title：Journal of clinical microbiology

authors： Maher M,Finnegan C,Collins E,Ward B,Carroll C,Cormican M

更新日期：2003-07-01 00:00:00

abstract：:Rhodotorula infections occur among patients with immunosuppression and/or central venous catheters. Using standardized methods (NCCLS M27-A), we determined the antifungal susceptibilities of 10 Rhodotorula bloodstream infection isolates. Patient information was collected for clinical correlation. The MICs of amphoteri...

journal_title：Journal of clinical microbiology

authors： Zaas AK,Boyce M,Schell W,Lodge BA,Miller JL,Perfect JR

更新日期：2003-11-01 00:00:00

abstract：:All medical and high-risk surgical patients were screened for methicillin-resistant Staphylococcus aureus colonization over 3.5 years. The sensitivities of nasal and rectal swabs were 68% and 62%, respectively. Naris and open-skin-site swabs detected 467 (74%) of 627 adult carriers identified. Rectal swabs detected an...

journal_title：Journal of clinical microbiology

authors： Currie A,Davis L,Odrobina E,Waldman S,White D,Tomassi J,Katz KC

更新日期：2008-09-01 00:00:00