Abstract:
:An enzymatic profile of 20 strains of Pseudomonas maltophilia was undertaken with conventional plate tests, API ZYM, and 4-methylumbelliferyl-conjugated substrates. All strains produced DNase, RNase, arbutinase, esterases and lipases, mucinase, acid and alkaline phosphatases, alkaline pyrophosphate diesterase, phosphoamidase, beta-glucosidase, leucine arylamidase, and acetatase and were hemolytic for horse, sheep, and rabbit blood. The majority of strains produced chitinase, hyaluronidase, albuminase, valine arylamidase, trypsin, alpha- and beta-glucosidases, and N-acetyl-beta-glucosaminidase. API ZYM and 4-methylumbelliferyl-conjugated substrate assays are rapid, simple, specific, and sensitive and may be useful as diagnostic aids in the identification of P. maltophilia and other pseudomonads.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
O'Brien M,Davis GHdoi
10.1128/JCM.16.3.417-421.1982subject
Has Abstractpub_date
1982-09-01 00:00:00pages
417-21issue
3eissn
0095-1137issn
1098-660Xjournal_volume
16pub_type
杂志文章abstract::To differentiate the Borrelia burgdorferi sensu lato genospecies, LightCycler real-time PCR was used for the fluorescence (SYBR Green I) melting curve analysis of borrelial recA gene PCR products. The specific melting temperature analyzed is a function of the GC/AT ratio, length, and nucleotide sequence of the amplifi...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.38.7.2756-2759.2000
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abstract::Nineteen reference and 156 clinical strains of the genus Nocardia belonging to 12 taxonomic groups were studied for restriction fragment length polymorphism (RFLP) by using an amplified 439-bp segment of the 65-kDa heat shock protein gene. Of 30 restriction endonucleases, digestion with MspI and then digestion with Bs...
journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.33.12.3096-3101.1995
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00064-11
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.39.8.2987-2990.2001
更新日期:2001-08-01 00:00:00
abstract::An evaluation of the serotyping system for Proteus rettgeri has been performed through its application to 561 human isolates of this species. Of these, 545 were typable and could be separated into 58 O serotypes. A group of isolates from one hospital, and clinically implicated in cross-infections, was found to belong ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:
更新日期:1976-04-01 00:00:00
abstract::This report describes the first well-documented reported case of the recovery of Blastomyces dermatitidis from the blood of a patient with disseminated blastomycosis. The isolate was recovered by using a lysis-centrifugation method and inhibitory mold agar after 6 days of incubation at 30 degrees C. One of six blood s...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.25.8.1421-1423.1987
更新日期:1987-08-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.26.8.1464-1470.1988
更新日期:1988-08-01 00:00:00
abstract::A simple method of storage and retrieval of serum samples for adenovirus serology is described. Complement-fixing antibodies remained stable for at least 5 years when stored at -10 degrees C or below. ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:
更新日期:1977-02-01 00:00:00
abstract::The Tigecycline Evaluation and Surveillance Trial (TEST) was designed to monitor global longitudinal changes in bacterial susceptibility to a panel of antimicrobial agents, including tigecycline. In this study, we examine susceptibility among Gram-positive isolates collected from pediatric patients globally between 20...
journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.20.5.990-992.1984
更新日期:1984-11-01 00:00:00
abstract::We tested 121 pneumococcal serogroup 6 isolates (including 30 serotype 6C and 24 serotype 6D isolates) by serotype-specific PCR and the Quellung reaction, using "old" and "new" pool B, factor 6b, and new factor 6d antisera. In combination with group B and other factor antisera, factor 6d antiserum can reliably identif...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00675-10
更新日期:2010-09-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.43.5.2047-2052.2005
更新日期:2005-05-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.23.5.987-991.1986
更新日期:1986-05-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.43.2.589-595.2005
更新日期:2005-02-01 00:00:00
abstract::Staphylococcus lugdunensis is a coagulase-negative staphylococcus that has several similarities to Staphylococcus aureus. S. lugdunensis is increasingly being recognized as a cause of prosthetic joint infection (PJI). The goal of the present retrospective cohort study was to determine the laboratory and clinical chara...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01769-09
更新日期:2010-05-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.42.6.2523-2529.2004
更新日期:2004-06-01 00:00:00
abstract::Human immunodeficiency virus type 1 serotype C was found in 545 of 712 Ethiopian patients by peptide enzyme immunoassay. Serotyping failed in 146 samples due to the absence of V3 antibodies or multiple reactivities. In 6 of 34 samples, discordant results were obtained by serotyping and genotyping, possibly due to dive...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.35.9.2419-2421.1997
更新日期:1997-09-01 00:00:00
abstract::The bacterial genus Gordona includes seven species of mycolic acid-containing actinomycetes which are cultured from environmental sources and rarely from clinical samples. They have been implicated in primary pulmonary diseases, nosocomial wound infections, and central nervous system infections in two immunocompromise...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.35.2.379-382.1997
更新日期:1997-02-01 00:00:00
abstract::The capsular-like envelope of Legionella pneumophila strains Togus 1 (serotype 2) and Philadelphia 1 (serotype 1) was isolated and purified by column chromatography on Sepharose 6B. Antibody raised in rabbits to these two antigenic materials did not cross-react in gel diffusion. Upon electrophoresis followed by gel di...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.13.4.637-642.1981
更新日期:1981-04-01 00:00:00
abstract::Epstein-Barr virus (EBV) infection is associated with a broad spectrum of disease. While quantification of EBV nucleic acid in the peripheral blood has been demonstrated to be useful for diagnosis and patient care, the optimal sample type and reporting format for such testing remain uncertain. Using quantitative real-...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.02308-06
更新日期:2007-07-01 00:00:00
abstract::CHROMagar Yersinia (CAY) is a new chromogenic medium for the presumptive detection of virulent Yersinia enterocolitica in stools. Based on a comparative analysis of 1,494 consecutive stools from hospitalized patients, CAY was found to be just as sensitive as the reference medium (cefsulodin-irgasan-novobiocin agar) bu...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.02903-12
更新日期:2013-04-01 00:00:00
abstract::The noninvasive diagnosis of amebic liver abscess is challenging, as most patients at the time of diagnosis do not have a concurrent intestinal infection with Entamoeba histolytica. Fecal testing for E. histolytica parasite antigen or DNA is negative in most patients. A real-time PCR assay was evaluated for detection ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00152-10
更新日期:2010-08-01 00:00:00
abstract::During an 8.5-year period (1974 to 1982), 56 Yersinia enterocolitica isolates from patients residing mainly in the New York City area were studied. Evaluation of these isolates revealed a marked increase in bacteremic episodes caused by strains of serogroups of O:3 and O:5,27 and the overall emergence of serogroup O:3...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.17.1.63-67.1983
更新日期:1983-01-01 00:00:00
abstract::The use of high-risk human papillomavirus (HPV) testing for surveillance and clinical applications is increasing globally, and it is important that tests are evaluated to ensure they are fit for this purpose. In this study, the performance of a new HPV genotyping test, the Papilloplex high-risk HPV (HR-HPV) test, was ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01687-17
更新日期:2018-02-22 00:00:00
abstract::The rates of infection with Fusarium molds are increasing, and a diverse number of Fusarium spp. belonging to different species complexes can cause infection. Conventional species identification in the clinical laboratory is time-consuming and prone to errors. We therefore evaluated whether matrix-assisted laser desor...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.02213-14
更新日期:2015-02-01 00:00:00
abstract::We compared culture results to investigate the influence of antimicrobial-loaded cement on sonicate fluid culture positivity for the diagnosis of prosthetic joint infection. Fifty-four subjects were assessed. The sensitivities of sonicate fluid culture were 77.8% (14 of 18) in subjects with an antimicrobial-loaded cem...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00516-16
更新日期:2016-06-01 00:00:00
abstract::Group A streptococcus (GAS) species cause bacterial pharyngitis in both adults and children. Early and accurate diagnosis of GAS is important for appropriate antibiotic therapy to prevent GAS sequalae. The Revogene Strep A molecular assay (Meridian Bioscience Canada Inc, Quebec City, QC, Canada) is an automated real-t...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01775-19
更新日期:2020-06-24 00:00:00
abstract::Recent studies suggest that a group of Chlamydia strains known as TWAR, which are now proposed to be a new species called Chlamydia pneumoniae, may be a frequent cause of respiratory disease in the United States and many other countries. Current serotesting methods do not allow rapid screening of large numbers of samp...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.27.12.2778-2783.1989
更新日期:1989-12-01 00:00:00
abstract::Two group A, subgroup I, rotavirus strains possessing "super short" RNA patterns were adapted to growth in MA-104 cells. Both produced marked cytopathic effect in primary culture. Reciprocal cross-neutralization titers, polypeptide analysis, and the serum neutralizing antibody response of an infected child suggested t...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.25.1.183-185.1987
更新日期:1987-01-01 00:00:00