Evaluation of a Proteus rettgeri O-serotyping system for epidemiological investigation.

abstract：:The distribution of Chlamydia trachomatis serovars and Neisseria gonorrhoeae coinfection was studied in a group of 100 C. trachomatis-positive males with urethritis in Greece. The serovar distribution revealed that apart from the predominant worldwide types E and F, the relatively uncommon type G is also prevalent. Go...

journal_title：Journal of clinical microbiology

authors： Papadogeorgakis H,Pittaras TE,Papaparaskevas J,Pitiriga V,Katsambas A,Tsakris A

更新日期：2010-06-01 00:00:00

abstract：:The diagnostic utility of Epstein-Barr virus (EBV) DNA detection in cerebrospinal fluid for the diagnosis of central nervous system lymphoma was evaluated with two different PCR assays to test a collection of cerebrospinal fluid samples from 24 AIDS patients with central nervous system disorders. A PCR assay amplifyin...

journal_title：Journal of clinical microbiology

authors： Arribas JR,Clifford DB,Fichtenbaum CJ,Roberts RL,Powderly WG,Storch GA

更新日期：1995-06-01 00:00:00

abstract：:Myxozoan spores were detected in fecal samples from three patients presenting with abdominal pain and/or diarrhea. The spores were identical to those of Myxobolus plectroplites, a previously described pathogen from the freshwater fish Plectroplites ambiguus. All patients had recently eaten fish caught from local water...

journal_title：Journal of clinical microbiology

authors： Boreham RE,Hendrick S,O'Donoghue PJ,Stenzel DJ

更新日期：1998-12-01 00:00:00

abstract：:Using PCR-based denaturing gradient gel electrophoresis analyses of oral bacterial samples in 20 mother-child dyads, this study demonstrated a high degree of similarity of bacterial compositions between the mothers and their children; the two may share as much as 94% of their oral bacterial spectra, including cariogen...

journal_title：Journal of clinical microbiology

authors： Li Y,Ismail AI,Ge Y,Tellez M,Sohn W

更新日期：2007-09-01 00:00:00

abstract：:Cryptococcus neoformans and Cryptococcus gattii are the main pathogenic species of invasive cryptococcosis among the Cryptococcus species. Taxonomic studies have shown that these two taxa have different genotypes or molecular types with biological and ecoepidemiological peculiarities. Matrix-assisted laser desorption ...

journal_title：Journal of clinical microbiology

authors： Siqueira LPM,Gimenes VMF,de Freitas RS,Melhem MSC,Bonfietti LX,da Silva AR Jr,Souza Santos LB,Motta AL,Rossi F,Benard G,de Almeida JN Jr

更新日期：2019-01-02 00:00:00

abstract：:Escherichia hermannii was isolated in pure culture from a patient with acute purulent conjunctivitis after a minor ocular injury. This is the first report of E. hermannii isolated as the sole pathogen from an infected site without prior antibiotic exposure, confirming the pathogenic potential of the microorganism. ...

journal_title：Journal of clinical microbiology

authors： Poulou A,Dimitroulia E,Markou F,Tsakris A

更新日期：2008-11-01 00:00:00

abstract：:We previously reported on the efficacy of the enzyme-linked immunoglobulin M capture immune complex (IC) biotinylated antigen assay (EMIBA) for the seroconfirmation of early Lyme disease and active infection with Borrelia burgdorferi. In earlier work we identified non-cross-reacting epitopes of a number of B. burgdorf...

journal_title：Journal of clinical microbiology

authors： Brunner M,Stein S,Mitchell PD,Sigal LH

更新日期：1998-04-01 00:00:00

abstract：:Simple enzymatic assays to detect heat-labile enterotoxins whose modes of action are similar to that of cholera toxin were evaluated. The assays are performed by using an artificial substrate, diethylamino benzylidine-aminoguanidine, which is an ADP-ribose acceptor. The product, formed in the presence of NAD+, can be ...

journal_title：Journal of clinical microbiology

authors： Narayanan J,Hartman PA,Graves DJ

更新日期：1989-11-01 00:00:00

abstract：:The iC-GPC Assay (iCubate, Huntsville, AL) is a qualitative multiplex test for the detection of five of the most common Gram-positive bacteria (Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumoniae, Enterococcus faecalis, and Enterococcus faecium) responsible for bacterial bloodstream infections,...

journal_title：Journal of clinical microbiology

authors： Granato PA,Unz MM,Widen RH,Silbert S,Young S,Heflin KL,Conover MS,Buchan BW,Ledeboer NA

更新日期：2018-08-27 00:00:00

abstract：:The pathogenicity of the fungus Geotrichum candidum in humans has not been clearly defined. A patient with acute leukemia who developed a fatal disseminated G. candidum infection while neutropenic is described. At autopsy examination, this patient was misdiagnosed on the basis of histopathology as having disseminated ...

journal_title：Journal of clinical microbiology

authors： Kassamali H,Anaissie E,Ro J,Rolston K,Kantarjian H,Fainstein V,Bodey GP

更新日期：1987-09-01 00:00:00

abstract：:Antiserum to a purified type R lipopolysaccharide antigen isolated from Neisseria gonorrhoeae was used in a slide agglutination test and compared with conventional carbohydrate utilization and fluorescent antibody tests to confirm the identity of laboratory cultures classified as typical or "atypical" N. gonorrhoeae. ...

journal_title：Journal of clinical microbiology

authors： Arko RJ,Wong KH,Peacock WL

更新日期：1979-04-01 00:00:00

abstract：:Sensitivity of the enzyme-linked immunosorbent assay for detecting serum antibodies to rotavirus was greatly enhanced when rotavirus particles were fragmented by chaotropic agents (NaSCN or guanidine hydrochloride) before adsorption of the antigens to the solid phase. For detecting fecal antibodies, the addition of fe...

journal_title：Journal of clinical microbiology

authors： Inouye S,Matsuno S,Yamaguchi H

更新日期：1984-02-01 00:00:00

abstract：:Three cases of neonatal infection caused by Enterobacter sakazakii are reported from the Department of Neonatal Intensive Care, the National University Hospital, Reykjavík, Iceland. These infections occurred during a 9-month period in 1986 and 1987. Two of the neonates, who were normal at birth, survived but were left...

journal_title：Journal of clinical microbiology

authors： Biering G,Karlsson S,Clark NC,Jónsdóttir KE,Lúdvígsson P,Steingrímsson O

更新日期：1989-09-01 00:00:00

abstract：:Reactivation of latent varicella-zoster virus (VZV), presenting as localized zoster or as disseminated infection, is a common and potentially serious complication in hematopoietic stem cell transplantation (HSCT) recipients. We retrospectively studied anti-VZV immunoglobulin G titers by the immune adherence hemaggluti...

journal_title：Journal of clinical microbiology

authors： Onozawa M,Hashino S,Takahata M,Fujisawa F,Kawamura T,Nakagawa M,Kahata K,Kondo T,Ota S,Tanaka J,Imamura M,Asaka M

更新日期：2006-12-01 00:00:00

abstract：:Although substantial epidemiologic evidence links Streptococcus mutans to caries, the pathobiology of caries may involve more complex communities of bacterial species. Molecular methods for bacterial identification and enumeration now make it possible to more precisely study the microbiota associated with dental carie...

journal_title：Journal of clinical microbiology

authors： Becker MR,Paster BJ,Leys EJ,Moeschberger ML,Kenyon SG,Galvin JL,Boches SK,Dewhirst FE,Griffen AL

更新日期：2002-03-01 00:00:00

abstract：:Paecilomyces variotii is a commonly occurring species in air and food, but it is also associated with many types of human infections and is among the emerging causative agents of opportunistic mycoses in immunocompromised hosts. Paecilomyces can cause hyalohyphomycosis, and two species, Paecilomyces lilacinus and P. v...

journal_title：Journal of clinical microbiology

authors： Houbraken J,Verweij PE,Rijs AJ,Borman AM,Samson RA

更新日期：2010-08-01 00:00:00

abstract：:The arylsulfatase isozymes of Mycobacterium fortuitum, M. peregrinum, M. chelonei subsp. chelonei, and M. chelonei subsp. abscessus were examined to determine the isozymal and immunological relationship among the members of the M. fortuitum complex. Cell extracts were subjected to electrophoresis on agarose and polyac...

journal_title：Journal of clinical microbiology

authors： Binko BP,Bartholomew WR

更新日期：1978-08-01 00:00:00

abstract：:A method is described for the routine preparation of sputum samples for detection of Mycobacterium tuberculosis by using polymerase chain reaction amplification. Liquefaction of sputum samples with NaOH and subsequent removal of inhibitors of the polymerase reaction with a 50% sucrose centrifugation step (5 min) in a ...

journal_title：Journal of clinical microbiology

authors： Victor T,du Toit R,van Helden PD

更新日期：1992-06-01 00:00:00

abstract：:The fluorescent amplified-fragment length polymorphism (FAFLP) assay potentially amplifies a unique set of genome fragments from each bacterial clone. It uses stringently hybridizing primers which carry a fluorescent label. Precise fragment sizing is achieved by the inclusion of an internal size standard in every lane...

journal_title：Journal of clinical microbiology

authors： Arnold C,Metherell L,Willshaw G,Maggs A,Stanley J

更新日期：1999-05-01 00:00:00

abstract：:A newly developed latex agglutination assay for the detection of genus-specific Leptospira antibodies in human sera was evaluated. The assay is performed by mixing, on an agglutination card, serum with equal volumes of stabilized antigen-coated, dyed test and control latex beads and is read within 2 min. The latex agg...

journal_title：Journal of clinical microbiology

authors： Smits HL,van der Hoorn MA,Goris MG,Gussenhoven GC,Yersin C,Sasaki DM,Terpstra WJ,Hartskeerl RA

更新日期：2000-03-01 00:00:00

abstract：:The mold Aureobasidium pullulans was isolated on several nutrient media from a splenic abscess in a patient with disseminated lymphoma. Examination of stained smears and paraffin sections revealed fungal structures characteristic of this organism. This is the first reported association of A. pullulans with an opportun...

journal_title：Journal of clinical microbiology

authors： Salkin IF,Martinez JA,Kemna ME

更新日期：1986-05-01 00:00:00

abstract：:The reported rate of detection of Chlamydia pneumoniae DNA within atherosclerotic lesions by PCR varies between 0 and 100%. In this study, identical sets of coded experimental atheroma samples (n = 15) and spiked controls (n = 5) were analyzed by 16 test methods in nine centers by means of PCR. The positive controls w...

journal_title：Journal of clinical microbiology

authors： Apfalter P,Blasi F,Boman J,Gaydos CA,Kundi M,Maass M,Makristathis A,Meijer A,Nadrchal R,Persson K,Rotter ML,Tong CY,Stanek G,Hirschl AM

更新日期：2001-02-01 00:00:00

abstract：:We cloned and characterized a phosphatidylcholine-hydrolyzing phospholipase C (PC-PLC) gene from Burkholderia pseudomallei. DNA sequence analysis of the gene indicated an open reading frame coding for 700 amino acids with a 34-amino-acid signal peptide. When cleaved, this yields a secreted 73-kDa mature protein. The d...

journal_title：Journal of clinical microbiology

authors： Korbsrisate S,Suwanasai N,Leelaporn A,Ezaki T,Kawamura Y,Sarasombath S

更新日期：1999-11-01 00:00:00

abstract：:A method was developed to obtain purified cryptosporidium oocysts from fecal samples. Oocysts were initially collected by centrifugation through a sucrose density gradient and further purified by passage through glass bead columns. The purified oocysts were antigenically active and sufficiently pure for immunological ...

journal_title：Journal of clinical microbiology

authors： Heyman MB,Shigekuni LK,Ammann AJ

更新日期：1986-04-01 00:00:00

abstract：:Replication of Sendai virus, pneumonia virus of mice, and SV5 was investigated in tracheal organ cultures from 2- to 4-day-old and 2- and 4-week-old hamsters, and viral infectivity in tracheal explants was compared with that in tissue culture monolayers. Explants from 2- to 4-day-old hamsters produced higher titers of...

journal_title：Journal of clinical microbiology

authors： Schiff LJ

更新日期：1976-09-01 00:00:00

abstract：:Mycobacterium tilburgii is a nonculturable nontuberculous mycobacterium identifiable only by molecular methods. We report a case of disseminated M. tilburgii infection illustrating the importance of 16S rRNA gene sequencing to determine the responsible mycobacterial pathogen and the difficulties in tailoring antimycob...

journal_title：Journal of clinical microbiology

authors： Temmerman S,Vandekerckhove L,Sermijn E,Vogelaers D,Claeys G,Vaneechoutte M,Cools P,Callens S

更新日期：2014-05-01 00:00:00

abstract：:Blood was cultured in two vacuum bottles containing Columbia broth. Filtered air was admitted to one bottle (aerobic); the unvented bottle was considered anaerobic. Cultures were incubated at 35 C until growth occurred or for at least 7 days. Of 744 organisms isolated, 50% were isolated from both bottles, 30% from the...

journal_title：Journal of clinical microbiology

authors： Blazevic DJ,Stemper JE,Matsen JM

更新日期：1975-02-01 00:00:00

abstract：:In the face of concerns over an influenza pandemic, identification of virulent influenza A virus isolates must be obtained quickly for effective responses. Rapid subtype identification, however, is difficult even in well-equipped virology laboratories or is unobtainable in the field under more austere conditions. Here...

journal_title：Journal of clinical microbiology

authors： Lodes MJ,Suciu D,Elliott M,Stover AG,Ross M,Caraballo M,Dix K,Crye J,Webby RJ,Lyon WJ,Danley DL,McShea A

更新日期：2006-04-01 00:00:00

abstract：:Molecular techniques such as spa typing and multilocus sequence typing use DNA sequence data for differentiating Staphylococcus aureus isolates. Although spa typing is capable of detecting both genetic micro- and macrovariation, it has less discriminatory power than the more labor-intensive pulsed-field gel electropho...

journal_title：Journal of clinical microbiology

authors： Koreen L,Ramaswamy SV,Naidich S,Koreen IV,Graff GR,Graviss EA,Kreiswirth BN

更新日期：2005-08-01 00:00:00

abstract：:Molecular typing of 246 Staphylococcus aureus isolates from unselected patients in Thailand showed that 10 (4.1%) were actually Staphylococcus argenteus. Contrary to the suggestion that S. argenteus is less virulent than S. aureus, we demonstrated comparable rates of morbidity, death, and health care-associated infect...

journal_title：Journal of clinical microbiology

authors： Thaipadungpanit J,Amornchai P,Nickerson EK,Wongsuvan G,Wuthiekanun V,Limmathurotsakul D,Peacock SJ

更新日期：2015-03-01 00:00:00