Simple latex agglutination assay for rapid serodiagnosis of human leptospirosis.

abstract：:Routine bacterial cultures of corneal scrapings from seven cats with either ulcerative feline keratitis, keratomalacia, or both yielded colonies which were identified by 16S rRNA gene sequencing as Mycoplasma felis (six cases) and Mycoplasma gateae (one case). Identification of the pathogens allowed the use of less em...

journal_title：Journal of clinical microbiology

authors： Gray LD,Ketring KL,Tang YW

更新日期：2005-07-01 00:00:00

abstract：:A Campylobacter fetus subsp. venerealis-specific 5' Taq nuclease PCR assay using a 3' minor groove binder-DNA probe (TaqMan MGB) was developed based on a subspecies-specific fragment of unknown identity (S. Hum, K. Quinn, J. Brunner, and S. L. On, Aust. Vet. J. 75:827-831, 1997). The assay specifically detected four C...

journal_title：Journal of clinical microbiology

authors： McMillen L,Fordyce G,Doogan VJ,Lew AE

更新日期：2006-03-01 00:00:00

abstract：:Candida albicans is an important cause of systemic fungal infections, and rapid diagnostics for identifying and differentiating C. albicans from other Candida species are critical for the timely application of appropriate antimicrobial therapy, improved patient outcomes, and pharmaceutical cost savings. In this work, ...

journal_title：Journal of clinical microbiology

authors： Kim HJ,Brehm-Stecher BF

更新日期：2015-02-01 00:00:00

abstract：:Two species of Bartonella, a novel Bartonella clarridgeiae-like bacterium and B. vinsonii subsp. berkhoffii, were isolated from rural dogs and gray foxes in northern California. A novel B. clarridgeiae-like species was isolated from 3 (1.7%) of 182 dogs and 22 (42%) of 53 gray foxes, while B. vinsonii subsp. berkhoffi...

journal_title：Journal of clinical microbiology

authors： Henn JB,Gabriel MW,Kasten RW,Brown RN,Theis JH,Foley JE,Chomel BB

更新日期：2007-08-01 00:00:00

abstract：:Of 532 strains of viridans streptococci isolated from clinical specimens, 517 were identified by using a scheme based on the work of Facklam (R. R. Facklam, J. Clin. Microbiol. 5:184-201, 1977). The strains were distributed among nine of the species described by Facklam and an additional group of physiologically homog...

journal_title：Journal of clinical microbiology

authors： Ruoff KL,Kunz LJ

更新日期：1982-05-01 00:00:00

abstract：:Low-speed centrifugation-mediated adsorption was evaluated as an enhancement of infectivity of clinical and laboratory strains of cytomegalovirus (CMV) occurring with cells grown in conventional culture tubes. The time required for reporting of primary isolates of CMV from urine specimens adsorbed onto monolayers of W...

journal_title：Journal of clinical microbiology

authors： Oefinger PE,Shawar RM,Loo SH,Tsai LT,Arnett JK

更新日期：1990-05-01 00:00:00

abstract：:Accreditation in Ontario, Canada, requires that licensed clinical laboratories participate in external quality assessment (also known as proficiency testing) and perform competency evaluation of their staff. To assess the extent of ongoing competency assessment practices, the Quality Management Program--Laboratory Ser...

journal_title：Journal of clinical microbiology

authors： Desjardins M,Fleming CA

更新日期：2014-08-01 00:00:00

abstract：:The Streptococcus bovis group has undergone significant taxonomic changes over the past 2 decades with the advent of new identification methods with higher discriminatory power. Although the current classification system is not yet embraced by all researchers in the field and debate remains over the performance of mol...

journal_title：Journal of clinical microbiology

authors： Dekker JP,Lau AF

更新日期：2016-07-01 00:00:00

abstract：:Thirty-seven bacterial clones producing human recombinant monoclonal antibody Fab fragments (rFabs) reactive to herpes simplex virus (HSV) antigens were selected from a human combinatorial antibody library constructed in a phage-display vector by a panning procedure against an HSV lysate. Thirty-four of the HSV-specif...

journal_title：Journal of clinical microbiology

authors： Cattani P,Rossolini GM,Cresti S,Santangelo R,Burton DR,Williamson RA,Sanna PP,Fadda G

更新日期：1997-06-01 00:00:00

abstract：:An unusual Enterocytozoon bieneusi genotype was found in seven guinea pigs and a 2-year-old child in the same household. The genetic uniqueness of the parasite, its wide occurrence in other guinea pigs in the community, and its absence in other children in the community suggest the possibility of zoonotic transmission...

journal_title：Journal of clinical microbiology

authors： Cama VA,Pearson J,Cabrera L,Pacheco L,Gilman R,Meyer S,Ortega Y,Xiao L

更新日期：2007-08-01 00:00:00

abstract：:Two strains of a previously undescribed Actinomyces-like bacterium were recovered in pure culture from infected root canals of teeth. Analysis by biochemical testing and polyacrylamide gel electrophoresis of whole-cell proteins indicated that the strains closely resembled each other phenotypically but were distinct fr...

journal_title：Journal of clinical microbiology

authors： Collins MD,Hoyles L,Kalfas S,Sundquist G,Monsen T,Nikolaitchouk N,Falsen E

更新日期：2000-09-01 00:00:00

abstract：:A novel recombinant baculovirus which expresses Ebola virus (EBO) nucleoprotein (NP) under the control of the cytomegalovirus immediate-early promoter was constructed. HeLa cells abortively infected with the baculovirus expressed EBO NP, and this was used as an immunofluorescent (IF) antigen to detect EBO immunoglobul...

journal_title：Journal of clinical microbiology

authors： Saijo M,Niikura M,Morikawa S,Kurane I

更新日期：2001-02-01 00:00:00

abstract：:A recently described medium (Haemophilus test medium [HTM]) for antimicrobial susceptibility testing of Haemophilus influenzae was evaluated in this study for broth microdilution testing of Streptococcus pneumoniae. A total of 137 clinical isolates was tested against 11 antimicrobial agents, using Mueller-Hinton broth...

journal_title：Journal of clinical microbiology

authors： Jorgensen JH,Maher LA,Howell AW

更新日期：1990-03-01 00:00:00

abstract：:Blood specimens collected from adult patients with suspected sepsis in four medical centers were inoculated into BACTEC Plus/F and BacT/Alert FAN aerobic culture bottles. Both bottles of 7,401 bottle pairs contained the prescribed blood volume of 8 to 12 ml. Bottles were incubated in their respective instruments for a...

journal_title：Journal of clinical microbiology

authors： Jorgensen JH,Mirrett S,McDonald LC,Murray PR,Weinstein MP,Fune J,Trippy CW,Masterson M,Reller LB

更新日期：1997-01-01 00:00:00

abstract：:During July 2000 and October 2001, a total of 595 clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) were collected from six medical centers distributed in northern, central, and southern Taiwan. Specimen sources included blood (n = 279), pus (n = 173), sputum (n = 94), body fluids (n = 21), cathe...

journal_title：Journal of clinical microbiology

authors： Huang YC,Su LH,Wu TL,Liu CE,Young TG,Chen PY,Hseuh PR,Lin TY

更新日期：2004-01-01 00:00:00

abstract：:Metarhizium viride has been associated with fatal systemic mycoses in chameleons, but subsequent data on mycoses caused by this fungus in reptiles are lacking. The aim of this investigation was therefore to obtain information on the presence of M. viride in reptiles kept as pets in captivity and its association with c...

journal_title：Journal of clinical microbiology

authors： Schmidt V,Klasen L,Schneider J,Hübel J,Pees M

更新日期：2017-03-01 00:00:00

abstract：:Three hundred ninety single sera from adults in Japan, Indonesia, Singapore, and Papua New Guinea were tested for antibodies to human calicivirus (HCV) by a radioimmunoassay blocking test. A high prevalence of antibodies was observed in samples collected in Japan and Southeast Asia. Of 240 serum specimens collected fr...

journal_title：Journal of clinical microbiology

authors： Nakata S,Chiba S,Terashima H,Nakao T

更新日期：1985-10-01 00:00:00

abstract：:An association between (unculturable) gastrospirillum-like organisms (GLO) and ulcerative lesions in the pars oesophagea in stomachs of swine has been claimed. In dogs GLO detected by microscopy may represent several Helicobacter species or subspecies. Therefore we investigated which Helicobacter spp. are present in s...

journal_title：Journal of clinical microbiology

authors： Roosendaal R,Vos JH,Roumen T,van Vugt R,Cattoli G,Bart A,Klaasen HL,Kuipers EJ,Vandenbroucke-Grauls CM,Kusters JG

更新日期：2000-07-01 00:00:00

abstract：:The emergence of ciprofloxacin-resistant Salmonella enterica serovar Choleraesuis in recent years has become an important public health issue in Taiwan. The resistant strains that cause human infections are considered to be from pigs. In this study, we characterized 157 swine and 42 human Salmonella serovar Choleraesu...

journal_title：Journal of clinical microbiology

authors： Chang CC,Lin YH,Chang CF,Yeh KS,Chiu CH,Chu C,Chien MS,Hsu YM,Tsai LS,Chiou CS

更新日期：2005-06-01 00:00:00

abstract：:The impact of the viability of Trichomonas vaginalis in urine on wet mount, culture, and PCR methods was assessed. To minimize the chance of false-negative results, urine specimens should be processed within 30 min of specimen collection and maintained at 37 degrees C, since temperature appears to affect the viability...

journal_title：Journal of clinical microbiology

authors： Shafir SC,Sorvillo FJ

更新日期：2006-10-01 00:00:00

abstract：:We developed a real-time PCR assay to detect single nucleotide polymorphisms associated with ciprofloxacin resistance in specimens submitted for nucleic acid amplification testing (NAAT). All three single nucleotide polymorphism (SNP) targets produced high sensitivity and specificity values. The presence of ≥2 SNPs wa...

journal_title：Journal of clinical microbiology

authors： Peterson SW,Martin I,Demczuk W,Bharat A,Hoang L,Wylie J,Allen V,Lefebvre B,Tyrrell G,Horsman G,Haldane D,Garceau R,Wong T,Mulvey MR

更新日期：2015-11-01 00:00:00

abstract：:A new immunochromatographic rapid test, Determine HIV-1/2, for the detection of antibodies to human immunodeficiency virus type 1 (HIV-1) and HIV-2 in human whole blood, serum, and plasma was evaluated. Determine HIV-1/2 is a sandwich immunoassay and uses a nitrocellulose strip with a capture site for the patient's re...

journal_title：Journal of clinical microbiology

authors： Arai H,Petchclai B,Khupulsup K,Kurimura T,Takeda K

更新日期：1999-02-01 00:00:00

abstract：:An enzyme immunoassay (enzyme-linked immunosorbent assay [ELISA]) to detect hamster antibody to toxins A and B of Clostridium difficile was developed in which toxin preparations are used to coat the solid phase. The specificity of the assay was supported by blocking tests with the toxin preparations and proteins from ...

journal_title：Journal of clinical microbiology

authors： Viscidi RP,Yolken RH,Laughon BE,Bartlett JG

更新日期：1983-08-01 00:00:00

abstract：:To evaluate the accuracy and utility of the Eiken Systek No. 1 (Eiken system; Eiken Chemical Co., Ltd., Tokyo, Japan), we conducted a clinical comparison, with 345 Enterobacteriaceae isolates, of the Eiken System with API 20E (Analytab Products, Inc., Plainview, N.Y.) and conventional methods. The Eiken system is a 21...

journal_title：Journal of clinical microbiology

authors： Castillo CB,Bruckner DA

更新日期：1984-10-01 00:00:00

abstract：:Nearly 400,000 people worldwide are known to have been infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) beginning in December 2019. The virus has now spread to over 168 countries including the United States, where the first cluster of cases was observed in the Seattle metropolitan area in Was...

journal_title：Journal of clinical microbiology

authors： Nalla AK,Casto AM,Huang MW,Perchetti GA,Sampoleo R,Shrestha L,Wei Y,Zhu H,Jerome KR,Greninger AL

更新日期：2020-05-26 00:00:00

abstract：:Formalin-ethyl ether sedimentation, Formalin-ethyl acetate sedimentation, and zinc sulfate flotation techniques were compared using over 250 clinical parasitology specimens. Fifty positive specimens were identified, and a variety of parasites, including amoebae, flagellates, cestodes, nematodes, and trematodes, were e...

journal_title：Journal of clinical microbiology

authors： Truant AL,Elliott SH,Kelly MT,Smith JH

更新日期：1981-05-01 00:00:00

abstract：:The BACTEC 9240 (Becton Dickinson Diagnostic Instrument Systems, Sparks, Md.) is a new continuous-monitoring blood culture system that uses internal, fluorescent-CO2 sensors. In a multicenter clinical trial, organism yield and times to detection with the prototype BACTEC 9240 system were compared with those of the BAC...

journal_title：Journal of clinical microbiology

authors： Nolte FS,Williams JM,Jerris RC,Morello JA,Leitch CD,Matushek S,Schwabe LD,Dorigan F,Kocka FE

更新日期：1993-03-01 00:00:00

abstract：:Sensitivity of the enzyme-linked immunosorbent assay for detecting serum antibodies to rotavirus was greatly enhanced when rotavirus particles were fragmented by chaotropic agents (NaSCN or guanidine hydrochloride) before adsorption of the antigens to the solid phase. For detecting fecal antibodies, the addition of fe...

journal_title：Journal of clinical microbiology

authors： Inouye S,Matsuno S,Yamaguchi H

更新日期：1984-02-01 00:00:00

abstract：:A cluster of methicillin-resistant Staphylococcus aureus (MRSA) infections among patients on an intensive care unit (ICU) was detected by routine infection control surveillance. In the period from 5 January to 22 June 1995, 10 patients on the ICU and a further 6 patients (5 on one ward that had received colonized pati...

journal_title：Journal of clinical microbiology

authors： Tambic A,Power EG,Talsania H,Anthony RM,French GL

更新日期：1997-12-01 00:00:00

abstract：:A clustered regularly interspaced short palindromic repeat (CRISPR) typing method has recently been developed and used for typing and subtyping of Salmonella spp., but it is complicated and labor intensive because it has to analyze all spacers in two CRISPR loci. Here, we developed a more convenient and efficient meth...

journal_title：Journal of clinical microbiology

authors： Li H,Li P,Xie J,Yi S,Yang C,Wang J,Sun J,Liu N,Wang X,Wu Z,Wang L,Hao R,Wang Y,Jia L,Li K,Qiu S,Song H

更新日期：2014-08-01 00:00:00