Effect of aerobic and anaerobic atmospheres on isolation of organisms from blood cultures.

abstract：:Several studies have identified nonsterile blood collection tubes as the reservoir for contaminating microorganisms in outbreaks of pseudobacteremia. Microorganisms are thought to be transferred from the nonsterile tubes to the blood culture tubes when blood is drawn for cultures, but the validity of this hypothesis h...

journal_title：Journal of clinical microbiology

authors： McNeil MM,Davis BJ,Anderson RL,Martone WJ,Solomon SL

更新日期：1985-07-01 00:00:00

abstract：:Infections with human immunodeficiency virus types 1 and 2 (HIV-1 and HIV-2, respectively) are zoonotic infections. In Africa, the potential exists for additional cross-species transmissions from at least 33 different species of simian immunodeficiency virus (SIV)-infected nonhuman primates (NHPs) through hunting and ...

journal_title：Journal of clinical microbiology

authors： Ndongmo CB,Switzer WM,Pau CP,Zeh C,Schaefer A,Pieniazek D,Folks TM,Kalish ML

更新日期：2004-11-01 00:00:00

abstract：:In Asia, bla(KPC) detection has been limited to East Asia and not yet seen in Southeast Asia. We report four bla(KPC-2)-containing Klebsiella pneumoniae isolates from two different hospitals in Singapore. All isolates belonged to strain type 11 (ST11) and were indistinguishable by pulsed-field gel electrophoresis (PFG...

journal_title：Journal of clinical microbiology

authors： Balm MN,Ngan G,Jureen R,Lin RT,Teo J

更新日期：2012-02-01 00:00:00

abstract：:As part of an ongoing investigation into nosocomial Legionella infections at Stanford University Medical Center (SUMC), we applied the technique of restriction endonuclease analysis (REA) to determine strain differences among three species, including Legionella pneumophila, Legionella dumoffii, and Legionella micdadei...

journal_title：Journal of clinical microbiology

authors： Tompkins LS,Troup NJ,Woods T,Bibb W,McKinney RM

更新日期：1987-10-01 00:00:00

abstract：:The utility of combined 16S rRNA (rrs) gene restriction fragment length polymorphism and plasmid profiles to differentiate between and within Campylobacter upsaliensis of human and canine origin was examined. Fourteen distinct rrs gene restriction fragment length polymorphs consisting of bands sized between 1.9 and 4....

journal_title：Journal of clinical microbiology

authors： Stanley J,Jones C,Burnens A,Owen RJ

更新日期：1994-07-01 00:00:00

abstract：:Ureaplasma parvum and Ureaplasma urealyticum are sexually transmitted, opportunistic pathogens of the human urogenital tract. There are 14 known serovars distributed between the two species. For decades, it has been postulated based upon limited data that virulence is related to serotype specificity. The results were ...

journal_title：Journal of clinical microbiology

authors： Xiao L,Paralanov V,Glass JI,Duffy LB,Robertson JA,Cassell GH,Chen Y,Waites KB

更新日期：2011-08-01 00:00:00

abstract：:Performance in proficiency testing (PT) schemes is an objective measure of a laboratory's best performance. We examined the performance of participants in two parasitology PT schemes in South Africa from 2004 through 2010. The average rates of acceptable scores over the period were 58% and 66% for the stool and blood ...

journal_title：Journal of clinical microbiology

authors： Poonsamy B,Dini L,Frean J

更新日期：2012-10-01 00:00:00

abstract：:We compared anaerobic lytic (AL) and pediatric aerobic resin-containing (Peds Plus/F) blood culture media for the isolation of Salmonella enterica serotype Typhi or Paratyphi A from children. The yields from AL and Peds Plus/F media were the same with equal volumes of blood, but recovery was faster from AL medium than...

journal_title：Journal of clinical microbiology

authors： Reller ME,Zaidi AK,Sultana S,Azeem S,Hanif B,Qureshi S,Hasan R,Bhutta Z,Akhter R,Goldmann DA

更新日期：2009-01-01 00:00:00

abstract：:A prospective study of Campylobacter jejuni infection was performed during a 6-month period in a cohort of 198 Chilean infants. Surveillance was based on biweekly home visits by a team of trained nurses. C. jejuni colonization was studied by culturing stool samples from diarrheal episodes and paired asymptomatic contr...

journal_title：Journal of clinical microbiology

authors： Figueroa G,Galeno H,Troncoso M,Toledo S,Soto V

更新日期：1989-05-01 00:00:00

abstract：:Comprehensive data on the prevalence of quinolone resistance in Mycobacterium tuberculosis clinical isolates in the United States are scarce. By use of a systematic population-based approach, M. tuberculosis strains from tuberculosis (TB) cases were collected in Harris County, TX, in 2007 to 2008. The susceptibilities...

journal_title：Journal of clinical microbiology

authors： El Sahly HM,Teeter LD,Jost KC Jr,Dunbar D,Lew J,Graviss EA

更新日期：2011-08-01 00:00:00

abstract：:In tests on specimens of dried blood, saliva, and urine from 55 human immunodeficiency virus (HIV)-seropositive and 55 HIV-seronegative patients, an immunoglobulin G capture enzyme immunoassay for the detection of antibodies to HIV types 1 and 2, GACELISA, gave 109 of 110, 109 of 109, and 109 of 110 correct results, r...

journal_title：Journal of clinical microbiology

authors： Thongcharoen P,Wasi C,Louisirirotchanakul S,Parry J,Connell J,Mortimer P

更新日期：1992-12-01 00:00:00

abstract：:The present study describes a method for amplifying DNA in Actinobacillus actinomycetemcomitans by using short, synthetic oligonucleotides of random sequence as primers in the polymerase chain reaction. Genomic DNA from each of 20 human isolates of A. actinomycetemcomitans was successfully amplified in a thermal cycle...

journal_title：Journal of clinical microbiology

authors： Preus HR,Haraszthy VI,Zambon JJ,Genco RJ

更新日期：1993-10-01 00:00:00

abstract：:Laboratory tests are essential for confirming sporadic cases and outbreaks of rubella. Detection of rubella virus is often necessary to confirm rubella cases and to identify specimens to be used to characterize wild-type rubella viruses. The sensitivities of four methods for detecting rubella virus infection using thr...

journal_title：Journal of clinical microbiology

authors： Zhu Z,Xu W,Abernathy ES,Chen MH,Zheng Q,Wang T,Zhang Z,Li C,Wang C,He W,Zhou S,Icenogle J

更新日期：2007-09-01 00:00:00

abstract：:A swine H3N2 (swH3N2) and pandemic (H1N1) 2009 (pH1N1) influenza A virus reassortant (swH3N2/pH1N1) was detected in Canadian swine at the end of 2010. Simultaneously, a similar virus was also detected in Canadian mink based on partial viral genome sequencing. The origin of the new swH3N2/pH1N1 viral genes was related ...

journal_title：Journal of clinical microbiology

authors： Tremblay D,Allard V,Doyon JF,Bellehumeur C,Spearman JG,Harel J,Gagnon CA

更新日期：2011-12-01 00:00:00

abstract：:A modified indirect hemagglutination test for the detection of treponemal antibodies was developed for use with finger-prick blood. By using paired serum and absorbed finger-prick blood from 58 patients from an area previously endemic for yaws and 12 patients without yaws, the modified hemagglutination test was compar...

journal_title：Journal of clinical microbiology

authors： Backhouse JL,Lee MH,Nesteroff SI,Hudson BJ,Hamilton PA

更新日期：1992-03-01 00:00:00

abstract：:An inexpensive, rapid, and simple colorimetric test for detection of bacteriuria is described. This test does not require bacterial growth and has the marked advantage of being able to quantify bacteria, even when the organisms are present in the urine of bacteriuric patients who are being treated with antibiotics. Th...

journal_title：Journal of clinical microbiology

authors： Wallis C,Melnick JL,Longoria CJ

更新日期：1981-09-01 00:00:00

abstract：:Klebsiella pneumoniae has been emerging as the leading cause of liver abscess in diabetic patients. Results of molecular typing of K. pneumoniae isolates from two siblings with liver abscess, their family members, and the environment suggest a possibility of cross infection of liver abscess by the fecal-oral route wit...

journal_title：Journal of clinical microbiology

authors： Chiu CH,Su LH,Wu TL,Hung IJ

更新日期：2001-06-01 00:00:00

abstract：:A modified multiple-locus variable-number tandem-repeat analysis (MMLVA) method was validated on Clostridium difficile-infected stool specimens from institutional outbreaks. The method allows simultaneous detection of toxin genes, deletions, and tandem repeats from cultured isolates or stool specimens. Results were us...

journal_title：Journal of clinical microbiology

authors： Broukhanski G,Low DE,Pillai DR

更新日期：2011-05-01 00:00:00

abstract：:In the face of concerns over an influenza pandemic, identification of virulent influenza A virus isolates must be obtained quickly for effective responses. Rapid subtype identification, however, is difficult even in well-equipped virology laboratories or is unobtainable in the field under more austere conditions. Here...

journal_title：Journal of clinical microbiology

authors： Lodes MJ,Suciu D,Elliott M,Stover AG,Ross M,Caraballo M,Dix K,Crye J,Webby RJ,Lyon WJ,Danley DL,McShea A

更新日期：2006-04-01 00:00:00

abstract：:A modified oxidase reagent, 1% tetramethyl-p-phenylenediamine in dimethyl sulfoxide, proved superior to the routinely used 1% aqueous tetramethyl-p-phenylenediamine dihydrochloride in detecting weakly oxidase-positive gram-negative bacteria after 24 h of growth on agar media (40 of 40 positive versus 22 of 40 positive...

journal_title：Journal of clinical microbiology

authors： Tarrand JJ,Gröschel DH

更新日期：1982-10-01 00:00:00

abstract：:Test strips for the detection of antibodies to human immunodeficiency virus type 1 were investigated using specimens from risk groups in Thailand (141 reactive; 445 nonreactive) in a local Thai laboratory. The diagnostic sensitivity and specificity were both 100%. Using a set of seroconversion panels, the sensitivity ...

journal_title：Journal of clinical microbiology

authors： Pagcharoenpol P,Burgess-Cassler A,Schramm W

更新日期：1996-04-01 00:00:00

abstract：:Quantitation of hepatitis C virus (HCV) RNA in plasma and serum samples is a costly procedure in both time and reagents. Additionally, cell-associated viral RNA may not be detected. This study evaluated the accuracy of HCV RNA quantitation in small-volume whole-blood (WB) samples, which would be appropriate for point-...

journal_title：Journal of clinical microbiology

authors： Bruns T,Steinmetzer K,Ermantraut E,Stallmach A

更新日期：2009-10-01 00:00:00

abstract：:To further investigate the pathogenic potential of different Borrelia burgdorferi genospecies, specimens from 27 patients with different manifestations of Lyme borreliosis were analyzed by PCR and reverse line blotting (RLB). In samples from Lyme arthritis patients, B. burgdorferi sensu stricto was predominantly ident...

journal_title：Journal of clinical microbiology

authors： Lünemann JD,Zarmas S,Priem S,Franz J,Zschenderlein R,Aberer E,Klein R,Schouls L,Burmester GR,Krause A

更新日期：2001-03-01 00:00:00

abstract：:We prospectively studied 156 patients with a diagnosis of community-acquired pneumonia requiring admission. Several respiratory specimens were obtained for the detection of Chlamydia pneumoniae by cell culture and PCR. Three serum samples were obtained from each patient. Serological diagnosis of a C. pneumoniae infect...

journal_title：Journal of clinical microbiology

authors： Verkooyen RP,Willemse D,Hiep-van Casteren SC,Joulandan SA,Snijder RJ,van den Bosch JM,van Helden HP,Peeters MF,Verbrugh HA

更新日期：1998-08-01 00:00:00

abstract：:The first case of osteomyelitis caused by Neosartorya pseudofischeri is reported. The patient, a 77-year-old male with a history of silicosis and tuberculosis, on X-ray examination revealed lytic lesions of L2 and L3 vertebrae suspicious for metastatic lesions. Histologic examination of biopsy specimens from vertebral...

journal_title：Journal of clinical microbiology

authors： Padhye AA,Godfrey JH,Chandler FW,Peterson SW

更新日期：1994-11-01 00:00:00

abstract：:We compared whole blood dried on filter paper to the standard assay with frozen cell-free plasma for use in the quantitation of the human immunodeficiency virus RNA load in blood. RNA values from filter paper, corrected for the hematocrit, gave results comparable to those of the standard assay in terms of sensitivity ...

journal_title：Journal of clinical microbiology

authors： Fiscus SA,Brambilla D,Grosso L,Schock J,Cronin M

更新日期：1998-01-01 00:00:00

abstract：:A series of clinical NDM-5-producing E. coli isolates obtained from two surveillance networks of carbapenem-producing Enterobacterales from 2018-2019, namely Switzerland (NARA) and Germany (SurvCARE), were analyzed. The 33 NDM-5-producing E. coli isolates were highly resistant to β-lactams including the novel ß-lactam...

journal_title：Journal of clinical microbiology

authors： Chakraborty T,Sadek M,Yao Y,Imirzalioglu C,Stephan R,Poirel L,Nordmann P

更新日期：2020-12-23 00:00:00

abstract：:Comparative studies on the detection of bovine serum immunoglobulin G antibodies to bluetongue virus with an enzyme-linked immunosorbent assay, an immunodiffusion method, and a serum neutralization assay demonstrated complete concordance between the enzyme-linked immunosorbent assay and the serum neutralization assay ...

journal_title：Journal of clinical microbiology

authors： Poli G,Stott J,Liu YS,Manning JS

更新日期：1982-01-01 00:00:00

abstract：:We compared the Gen-Probe transcription-mediated amplification assay (AMP CT), the Abbott LCx assay, and the Roche COBAS AMPLICOR assay for the detection of Chlamydia trachomatis in a mixed population in urine samples. First-void urine, urethral specimens, and cervical specimens in females were obtained from 1,000 pat...

journal_title：Journal of clinical microbiology

authors： Goessens WH,Mouton JW,van der Meijden WI,Deelen S,van Rijsoort-Vos TH,Lemmens-den Toom N,Verbrugh HA,Verkooyen RP

更新日期：1997-10-01 00:00:00

abstract：:PCR of a Candida albicans cytochrome P-450 lanosterol-alpha-demethylase (P450-L1A1) gene segment is a rapid and sensitive method of detection in clinical specimens. This enzyme is a target for azole antifungal action. In order to directly detect and identify the clinically most important species of Candida, we cloned ...

journal_title：Journal of clinical microbiology

authors： Burgener-Kairuz P,Zuber JP,Jaunin P,Buchman TG,Bille J,Rossier M

更新日期：1994-08-01 00:00:00