Controlled evaluation of Bactec Peds Plus/F and Bactec lytic/10 anaerobic/F media for isolation of Salmonella enterica serovars typhi and paratyphi A from blood.

abstract：:It has been shown by analysis with monoclonal and polyclonal antibodies that outer surface protein C (OspC) of Borrelia burgdorferi sensu lato is highly heterogeneous. To determine if the heterogeneity has a genetic basis, the genes of 18 different B. burgdorferi sensu lato strains have been amplified by PCR, cloned, ...

journal_title：Journal of clinical microbiology

authors： Jauris-Heipke S,Liegl G,Preac-Mursic V,Rössler D,Schwab E,Soutschek E,Will G,Wilske B

更新日期：1995-07-01 00:00:00

abstract：:We compared 75 nontypeable (NT) Haemophilus influenzae isolates by pulsed-field gel electrophoresis (PFGE), enterobacterial repetitive intergenic consensus (ERIC)-PCR, and automated ribotyping. PFGE was the most discriminatory of the techniques. ERIC-PCR provides a useful screen but should not replace other techniques...

journal_title：Journal of clinical microbiology

authors： Pettigrew MM,Foxman B,Ecevit Z,Marrs CF,Gilsdorf J

更新日期：2002-02-01 00:00:00

abstract：:Collecting whole blood on filter paper simplifies the processing, transport, and storage of specimens used for the diagnosis of human immunodeficiency virus type 1 (HIV-1) and other tests. Specimens may be collected in tropical or rural areas with minimal facilities for handling specimens. To compare simulated tropica...

journal_title：Journal of clinical microbiology

authors： Mitchell C,Jennings C,Brambilla D,Aldrovandi G,Amedee AM,Beck I,Bremer JW,Coombs R,Decker D,Fiscus S,Fitzgibbon J,Luzuriaga K,Moye J,Palumbo P,Reichelderfer P,Somasundaran M,Stevens W,Frenkel L,Dried Blood Spot Workin

更新日期：2008-09-01 00:00:00

abstract：:An accurate, rapid, and inexpensive method was developed for detecting and enumerating bacteria adherent to Foley urinary catheters based on malachite green staining of acridine orange-prestained specimens. This method has proven to be quick and reliable and will find application in quantitative studies of biomaterial...

journal_title：Journal of clinical microbiology

authors： Ladd TI,Schmiel D,Nickel JC,Costerton JW

更新日期：1985-06-01 00:00:00

abstract：:The development of porcine epidemic diarrhea virus (PEDV) antibody-based assays is important for detecting infected animals, confirming previous virus exposure, and monitoring sow herd immunity. However, the potential cross-reactivity among porcine coronaviruses is a major concern for the development of pathogen-speci...

journal_title：Journal of clinical microbiology

authors： Gimenez-Lirola LG,Zhang J,Carrillo-Avila JA,Chen Q,Magtoto R,Poonsuk K,Baum DH,Piñeyro P,Zimmerman J

更新日期：2017-05-01 00:00:00

abstract：:The human pathogen Staphylococcus aureus is isolated and characterized using traditional culture and sensitivity methodologies that are slow and offer limited information on the organism. In contrast, DNA microarray technology can provide detailed, clinically relevant information on the isolate by detecting the presen...

journal_title：Journal of clinical microbiology

authors： Spence RP,Wright V,Ala-Aldeen DA,Turner DP,Wooldridge KG,James R

更新日期：2008-05-01 00:00:00

abstract：:A clinical evaluation of the Amplicor polymerase chain reaction (PCR) assay for the detection of Chlamydia trachomatis in endocervical swabs (Roche Molecular Systems, Branchburg, N.J.) is described. This new clinical system used one-step sample preparation, amplification with biotinylated cryptic plasmid primer pairs ...

journal_title：Journal of clinical microbiology

authors： Bass CA,Jungkind DL,Silverman NS,Bondi JM

更新日期：1993-10-01 00:00:00

abstract：:A 115-kDa exoantigen produced by Cryptococcus neoformans recognized by the previously described murine monoclonal antibody 7C9 has been purified from culture filtrate by a combination of membrane ultrafiltration, isoelectric focusing, and preparative gel electrophoresis. It is produced in late-log-phase cultures and i...

journal_title：Journal of clinical microbiology

authors： Hamilton AJ,Goodley J

更新日期：1993-02-01 00:00:00

abstract：:We evaluated a new hepatitis C virus RNA assay based on one-stage PCR followed by liquid hybridization with an oligonucleotide probe and compared it with nested-set PCR. The one-stage and nested-set PCR assays had identical sensitivities in analytical experiments and showed 100% concordance when clinical specimens wer...

journal_title：Journal of clinical microbiology

authors： Gretch DR,Wilson JJ,Carithers RL Jr,dela Rosa C,Han JH,Corey L

更新日期：1993-02-01 00:00:00

abstract：:A proficiency testing scheme for the leptospirosis microscopic agglutination test was provided to 37 laboratories in 23 countries in 2002 (round 1) and to 60 laboratories in 34 countries in 2003 (round 2). Thirty-four laboratories participated in both rounds. Each panel consisted of five rabbit serum samples, four of ...

journal_title：Journal of clinical microbiology

authors： Chappel RJ,Goris M,Palmer MF,Hartskeerl RA

更新日期：2004-12-01 00:00:00

abstract：:The Gen-Probe Amplified Mycobacterium tuberculosis direct test is widely used in the diagnosis of tuberculosis. Specimens may contain amplification inhibitors, potentially leading to false-negative results if unrecognized. We report a failure to detect inhibition, despite adherence to the inhibition testing guidelines...

journal_title：Journal of clinical microbiology

authors： Kim JY,Ferraro MJ,Branda JA

更新日期：2009-09-01 00:00:00

abstract：:We have developed and evaluated a new method to quantify human immunodeficiency virus type 2 (HIV-2) proviral DNA based on LightCycler real-time PCR. The assay has a detection limit of 5 copies/10(5) peripheral blood mononuclear cells (PBMC) and is insensitive to HIV-2 strain variability: HIV-2 subtypes A and B are bo...

journal_title：Journal of clinical microbiology

authors： Damond F,Descamps D,Farfara I,Telles JN,Puyeo S,Campa P,Leprêtre A,Matheron S,Brun-Vezinet F,Simon F

更新日期：2001-12-01 00:00:00

abstract：:To determine that susceptibility of AMPLICOR Chlamydia trachomatis PCR to inhibitory factors possibly present in cervical specimens, we obtained cervical specimens from 200 gynecology patients attending our outpatient clinic. The prevalence of C. trachomatis infection was 4.1%, as determined by cell culture. All AMPLI...

journal_title：Journal of clinical microbiology

authors： Verkooyen RP,Luijendijk A,Huisman WM,Goessens WH,Kluytmans JA,van Rijsoort-Vos JH,Verbrugh HA

更新日期：1996-12-01 00:00:00

abstract：:The NCCLS M38-A document does not describe guidelines for testing caspofungin acetate (MK-0991) and other echinocandins against molds. This study evaluated the susceptibilities of 200 isolates of Aspergillus fumigatus, A. flavus, A. nidulans, A. niger, and A. terreus to caspofungin (MICs and minimum effective concentr...

journal_title：Journal of clinical microbiology

authors： Espinel-Ingroff A

更新日期：2003-01-01 00:00:00

abstract：:Clostridium perfringens is an important pathogen of animals and humans and is the causative agent of necrotic enteritis (NE) in poultry. This study focuses on the typing of intestinal C. perfringens isolates (n = 61) from outbreaks of NE collected from several areas of Southern Ontario, using a recently developed mult...

journal_title：Journal of clinical microbiology

authors： Chalmers G,Bruce HL,Hunter DB,Parreira VR,Kulkarni RR,Jiang YF,Prescott JF,Boerlin P

更新日期：2008-12-01 00:00:00

abstract：:The purpose of this study was to evaluate the performance of a herpes simplex virus (HSV) type 1-specific anti-glycoprotein C-1 monoclonal antibody (MAb) and a type 2-specific anti-glycoprotein G-2 MAb for typing of 2,400 clinical HSV-1 isolates and 2,400 clinical HSV-2 isolates, respectively, using an enzyme immunoas...

journal_title：Journal of clinical microbiology

authors： Liljeqvist JA,Svennerholm B,Bergström T

更新日期：1999-08-01 00:00:00

abstract：:Treatment of wild rats from a known scrub typhus focus in the Philippines with cyclophosphamide did not significantly enhance the isolation rate of Rickettsia tsutsugamushi using mouse inoculation techniques. Similarly, cyclophosphamide treatment of mice inoculated with organs of wild rats did not increase the recover...

journal_title：Journal of clinical microbiology

authors： van Peenen PF,Ho CM,See R

更新日期：1977-01-01 00:00:00

abstract：:We prospectively studied 156 patients with a diagnosis of community-acquired pneumonia requiring admission. Several respiratory specimens were obtained for the detection of Chlamydia pneumoniae by cell culture and PCR. Three serum samples were obtained from each patient. Serological diagnosis of a C. pneumoniae infect...

journal_title：Journal of clinical microbiology

authors： Verkooyen RP,Willemse D,Hiep-van Casteren SC,Joulandan SA,Snijder RJ,van den Bosch JM,van Helden HP,Peeters MF,Verbrugh HA

更新日期：1998-08-01 00:00:00

abstract：:Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has recently been described as a fast and inexpensive method for the identification of mycobacteria. Although mycobacteria require extraction prior to MALDI-TOF MS analysis, previously published protocols have been relatively c...

journal_title：Journal of clinical microbiology

authors： Mather CA,Rivera SF,Butler-Wu SM

更新日期：2014-01-01 00:00:00

abstract：:Large hospital-based clinical laboratories must be prepared to rapidly investigate potential infectious disease outbreaks. To challenge the ability of our molecular diagnostics laboratory to use whole-genome sequencing in a potential outbreak scenario and identify impediments to these efforts, we studied 84 invasive s...

journal_title：Journal of clinical microbiology

authors： Olsen RJ,Fittipaldi N,Kachroo P,Sanson MA,Long SW,Como-Sabetti KJ,Valson C,Cantu C,Lynfield R,Van Beneden C,Beres SB,Musser JM

更新日期：2014-12-01 00:00:00

abstract：:Rapid diagnostic tests (RDTs) represent important tools to diagnose malaria infection. To improve understanding of the variable performance of RDTs that detect the major target in Plasmodium falciparum, namely, histidine-rich protein 2 (HRP2), and to inform the design of better tests, we undertook detailed mapping of ...

journal_title：Journal of clinical microbiology

authors： Lee N,Gatton ML,Pelecanos A,Bubb M,Gonzalez I,Bell D,Cheng Q,McCarthy JS

更新日期：2012-04-01 00:00:00

abstract：:In the SIGNAL (Oxoid Ltd., Basingstoke, United Kingdom) blood culture system, gas produced by bacterial metabolism displaces medium from the culture bottle into an upper reservoir via a hollow needle. Displacement of media may provide a visual indication of the presence of both aerobic and anaerobic organisms in a sin...

journal_title：Journal of clinical microbiology

authors： Trombley C,Anderson JD

更新日期：1987-11-01 00:00:00

abstract：:We conducted a molecular epidemiology study on 120 Mycobacterium tuberculosis isolates from patients presenting pulmonary tuberculosis (TB) in Burkina Faso. Classical antibiogram studies and genetic characterization, using mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) typing and ...

journal_title：Journal of clinical microbiology

authors： Godreuil S,Torrea G,Terru D,Chevenet F,Diagbouga S,Supply P,Van de Perre P,Carriere C,Bañuls AL

更新日期：2007-03-01 00:00:00

abstract：:At the Islet Isolation Laboratory of the Scottish National Blood Transfusion Service, manual sterility testing data show that contamination rates are 57.7% for pancreas transport fluid, 4.3% for postpurification islet samples, and 0% for pretransplant islet samples. This pilot study presents the BacT/Alert System as a...

journal_title：Journal of clinical microbiology

authors： Murray L,McGowan N,Fleming J,Bailey L

更新日期：2014-10-01 00:00:00

abstract：:The importance of euparal as a permanent mounting medium for eggs of parasites and the process of making permanent slides are analyzed and discussed in detail. Studies show that helminth eggs mounted in euparal exhibit excellent optical and drying properties. Euparal also can be used to identify proglottids of Taenia ...

journal_title：Journal of clinical microbiology

authors： Berlin OG,Miller MJ

更新日期：1980-11-01 00:00:00

abstract：:A selective medium (CFAT) was developed for the detection and enumeration of Actinomyces viscosus and Actinomyces naeslundii in dental plaque. Neutral acriflavin and potassium tellurite were used in combination with the known selective agents cadmium and fluoride to eliminate most of the competing plaque flora. Compos...

journal_title：Journal of clinical microbiology

authors： Zylber LJ,Jordan HV

更新日期：1982-02-01 00:00:00

abstract：:Rotavirus serotype G9 is recognized as the most widespread of the emerging serotypes, emerging since 1996 as a frequent cause of severe acute gastroenteritis in children from many countries covering all continents of the world. This study characterized serotype G9 strains collected in three widely separated Australian...

journal_title：Journal of clinical microbiology

authors： Kirkwood C,Bogdanovic-Sakran N,Palombo E,Masendycz P,Bugg H,Barnes G,Bishop R

更新日期：2003-08-01 00:00:00

abstract：:Pneumococcus (Streptococcus pneumoniae) remains a significant cause of morbidity and mortality, especially among those at the extremes of age. Its capsular polysaccharide is essential for systemic virulence. Over 90 serologically distinct pneumococcal capsular polysaccharides (serotypes) are recognized, but they are u...

journal_title：Journal of clinical microbiology

authors： Geno KA,Saad JS,Nahm MH

更新日期：2017-05-01 00:00:00

abstract：:Borrelia lusitaniae is a species within the complex Borrelia burgdorferi sensu lato and is infrequently isolated in Europe. In contrast, this species is by far the most predominant in North Africa and in Portugal. In this study, we analyzed the genetic diversity, at several loci, of a large population of isolates from...

journal_title：Journal of clinical microbiology

authors： Younsi H,Sarih M,Jouda F,Godfroid E,Gern L,Bouattour A,Baranton G,Postic D

更新日期：2005-04-01 00:00:00

abstract：:Real-time PCR shows the widespread presence of Coxiella burnetii DNA in a broad range of commercially available milk and milk products. MLVA genotyping shows that this is the result of the presence of a predominant C. burnetii genotype in the dairy cattle population. ...

journal_title：Journal of clinical microbiology

authors： Tilburg JJ,Roest HJ,Nabuurs-Franssen MH,Horrevorts AM,Klaassen CH

更新日期：2012-06-01 00:00:00