Characterization of Borrelia lusitaniae isolates collected in Tunisia and Morocco.

abstract：:PCR-amplified 16S rRNA gene sequences were obtained directly from tissue specimens from eight cats with presumptive feline leprosy. Acid-fast bacilli were observed in sections from all eight specimens, but culture for mycobacteria was successful for one specimen only. Analysis of the V2 variable region of each 16S rRN...

journal_title：Journal of clinical microbiology

authors： Hughes MS,Ball NW,Beck LA,de Lisle GW,Skuce RA,Neill SD

更新日期：1997-10-01 00:00:00

abstract：:Better detection of minority human immunodeficiency virus type 1 (HIV-1) populations containing gene mutations may improve the usefulness of antiretroviral resistance testing for clinical management. Molecular cloning of HIV-1 PCR products which might improve minority detection can be slow and difficult, and commercia...

journal_title：Journal of clinical microbiology

authors： Martinez-Picado J,Sutton L,De Pasquale MP,Savara AV,D'Aquila RT

更新日期：1999-09-01 00:00:00

abstract：:Using PCR-based denaturing gradient gel electrophoresis analyses of oral bacterial samples in 20 mother-child dyads, this study demonstrated a high degree of similarity of bacterial compositions between the mothers and their children; the two may share as much as 94% of their oral bacterial spectra, including cariogen...

journal_title：Journal of clinical microbiology

authors： Li Y,Ismail AI,Ge Y,Tellez M,Sohn W

更新日期：2007-09-01 00:00:00

abstract：:Common causes of chronic diarrhea among travelers worldwide include protozoan parasites. The majority of parasitic infections are caused by Giardia duodenalis, Entamoeba histolytica, Cryptosporidium parvum, and Cryptosporidium hominis Similarly, these species cause the majority of parasitic diarrhea acquired in the Un...

journal_title：Journal of clinical microbiology

authors： Madison-Antenucci S,Relich RF,Doyle L,Espina N,Fuller D,Karchmer T,Lainesse A,Mortensen JE,Pancholi P,Veros W,Harrington SM

更新日期：2016-11-01 00:00:00

abstract：:Broth microdilution and disk diffusion susceptibility testing were performed on 73 strains of Pasteurella species isolated from human infections and on five American Type Culture Collection strains of Pasteurella species. Both methods appear reliable for testing susceptibilities of Pasteurella species. ...

journal_title：Journal of clinical microbiology

authors： Citron DM,Warren YA,Fernandez HT,Goldstein MA,Tyrrell KL,Goldstein EJ

更新日期：2005-05-01 00:00:00

abstract：:The first vector-borne Capnocytophaga canimorsus sepsis case is presented. An immunocompetent male who denied any contact with canines and who worked in a sawmill was bitten on his neck by a large pine weevil (Hylobius abietis L.; Coleoptera: Curculionidae). Bacteriological diagnosis was confirmed by 16S rRNA gene seq...

journal_title：Journal of clinical microbiology

authors： Tuuminen T,Viiri H,Vuorinen S

更新日期：2014-07-01 00:00:00

abstract：:An evaluation was undertaken to determine the optimal method for the in vitro susceptibility testing of 26 Nocardia asteroides complex isolates to the following antimicrobial agents: amikacin, ampicillin, amoxicillin-clavulanate, ceftriaxone, ciprofloxacin, erythromycin, imipenem, minocycline, and trimethoprim-sulfame...

journal_title：Journal of clinical microbiology

authors： Ambaye A,Kohner PC,Wollan PC,Roberts KL,Roberts GD,Cockerill FR 3rd

更新日期：1997-04-01 00:00:00

abstract：:Of 4,000 endocervical specimens tested with the Chlamydiazyme enzyme-linked immunosorbent assay (Abbott Laboratories), 233 (5.8%) gave positive results (A492 above the cutoff), which were confirmed with a blocking reagent (Abbott). An additional 34 specimens (14.6%) with chlamydial antigen were detected and confirmed ...

journal_title：Journal of clinical microbiology

authors： Kellogg JA,Seiple JW,Stroll ES

更新日期：1993-06-01 00:00:00

abstract：:Restriction fragment length polymorphism and hybridization of DNA extracted from Mycobacterium tuberculosis, nontuberculous mycobacteria, and nonmycobacterial species with a probe derived from IS6110 confirmed that IS6110 was specific to M. tuberculosis complex. In addition, DNA amplification with IS6110-specific prim...

journal_title：Journal of clinical microbiology

authors： Githui WA,Wilson SM,Drobniewski FA

更新日期：1999-04-01 00:00:00

abstract：:We report a failure of the real-time reverse transcriptase PCR H7 subtyping protocol currently used in national avian influenza surveillance programs. Significant substitutions in primer and probe target sequences were identified, especially in wild bird viruses. The protocol, originally designed for detecting H7 infl...

journal_title：Journal of clinical microbiology

authors： Xing Z,Cardona C,Dao P,Crossley B,Hietala S,Boyce W

更新日期：2008-05-01 00:00:00

abstract：:The in vitro susceptibilities of 66 molecularly identified strains of the Mucorales to eight antifungals (amphotericin B, terbinafine, itraconazole, posaconazole, voriconazole, caspofungin, micafungin, and 5-fluorocytosine) were tested. Molecular phylogeny was reconstructed based on the nuclear ribosomal large subunit...

journal_title：Journal of clinical microbiology

authors： Vitale RG,de Hoog GS,Schwarz P,Dannaoui E,Deng S,Machouart M,Voigt K,van de Sande WW,Dolatabadi S,Meis JF,Walther G

更新日期：2012-01-01 00:00:00

abstract：:A nontoxigenic strain of Vibrio cholerae O group 1 was isolated in Florida from the stool of a patient with severe diarrhea. The strain had the same hemolytic and unique phage-sensitivity pattern as all toxigenic isolates from recent cases of cholera in Texas and Louisiana. Identical strains were transiently isolated ...

journal_title：Journal of clinical microbiology

authors： Morris JG Jr,Picardi JL,Lieb S,Lee JV,Roberts A,Hood M,Gunn RA,Blake PA

更新日期：1984-02-01 00:00:00

abstract：:A total of 800 colonies suggestive of Salmonella, Shigella, or Yersinia species isolated on stool differential agar media were inoculated onto both conventional biochemical test media (triple sugar iron agar, urea agar, and phenylalanine agar) and Entero Pathogen Screen cards of the AutoMicrobic system (Vitek Systems,...

journal_title：Journal of clinical microbiology

authors： Villasante PA,Agulla A,Merino FJ,Pérez T,Ladrón de Guevara C,Velasco AC

更新日期：1987-03-01 00:00:00

abstract：:We determined the frequency with which human immunodeficiency virus (HIV) peripheral blood mononuclear cell cultures convert from positive to negative in subjects enrolled in a substudy of AIDS Clinical Trials Group (ACTG) 320, which compared the efficacy of treatment with a combination of indinavir, zidovudine, and l...

journal_title：Journal of clinical microbiology

authors： Demeter LM,Bosch RJ,Coombs RW,Fiscus S,Bremer J,Johnson VA,Erice A,Jackson JB,Spector SA,Squires KM,Fischl MA,Hughes MD,Hammer SM

更新日期：2002-06-01 00:00:00

abstract：:We report on a novel strategy for the detection of mRNA targets derived from Cryptosporidium parvum oocysts by the use of oligonucleotide-gold nanoparticles. Gold nanoparticles are functionalized with oligonucleotides which are complementary to unique sequences present on the heat shock protein 70 (HSP70) DNA/RNA targ...

journal_title：Journal of clinical microbiology

authors： Javier DJ,Castellanos-Gonzalez A,Weigum SE,White AC Jr,Richards-Kortum R

更新日期：2009-12-01 00:00:00

abstract：:There are several convenient and accurate molecular assays to detect respiratory bacterial infection. The NeoPlex RB-8 detection kit (NeoPlex RB-8) is a new multiplex real-time PCR assay that simultaneously detects Streptococcus pneumoniae, Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila, Haemo...

journal_title：Journal of clinical microbiology

authors： Kim JW,Hong SS,Lee IS,Chi HY,Kim SO,Kim HN,Hong SP

更新日期：2020-01-28 00:00:00

abstract：:This study assesses human papillomavirus (HPV) detection and genotyping in self-sampled genital smears applied to an indicating FTA elute cartridge (FTA cartridge). The study group consisted of 96 women, divided into two sample sets. All samples were analyzed by the HPV SPF(10)-Line Blot 25. Set 1 consisted of 45 wome...

journal_title：Journal of clinical microbiology

authors： Lenselink CH,de Bie RP,van Hamont D,Bakkers JM,Quint WG,Massuger LF,Bekkers RL,Melchers WJ

更新日期：2009-08-01 00:00:00

abstract：:A total of 563 specimens (234 dermal and 329 genital swabs) from patients suspected of having herpes simplex virus (HSV) infections were processed using two different extraction methods (the MagNA Pure LC system and the swab extraction tube system [SETS]); HSV DNA was amplified by LightCycler PCR. HSV DNA was detected...

journal_title：Journal of clinical microbiology

authors： Issa NC,Espy MJ,Uhl JR,Harmsen WS,Mandrekar JN,Gullerud RE,Davis MD,Smith TF

更新日期：2005-03-01 00:00:00

abstract：:Sentinel mouse seroconversion to infectious agents is critical for laboratory animal facility disease monitoring. We report spontaneous emergence of non-sex-linked agammaglobulinemia with B-cell deficiency and cutaneous Staphylococcus aureus granulomatosis (botryomycosis) in a cohort of related Swiss Webster sentinel ...

journal_title：Journal of clinical microbiology

authors： Bridgeford EC,Fox JG,Nambiar PR,Rogers AB

更新日期：2008-05-01 00:00:00

abstract：:A screening assay for phospholipase C using a chromogenic substrate incorporated into agar medium is described. The assay directly visualizes phospholipase C activity of mycoplasma lysates and membranes on agar plates, or the activity may be measured by spectrophotometry. The results from the assay confirm the presenc...

journal_title：Journal of clinical microbiology

authors： De Silva NS,Quinn PA

更新日期：1987-04-01 00:00:00

abstract：:In recent years testing responsibilities for high-consequence pathogens have been expanded from national reference laboratories into networks of local and regional laboratories in order to support enhanced disease surveillance and to test for surge capacity. This movement of testing of select agents and high-consequen...

journal_title：Journal of clinical microbiology

authors： Hietala SK,Crossley BM

更新日期：2006-01-01 00:00:00

abstract：:Twenty-nine Borrelia burgdorferi isolates, obtained from dusky-footed wood rats (Neotoma fuscipes) and California kangaroo rats (Dipodomys californicus) in California, were analyzed genetically. Chromosomal DNA was examined by restriction endonuclease analysis (REA) and gene probe restriction fragment length polymorph...

journal_title：Journal of clinical microbiology

authors： Zingg BC,Brown RN,Lane RS,LeFebvre RB

更新日期：1993-12-01 00:00:00

abstract：:Real-time PCR with melting curve analysis of PCR products is a rapid procedure for detecting and genotyping herpes simplex virus (HSV). When testing mucocutaneous samples for HSV by a real-time PCR assay targeting the DNA polymerase gene, we found that some PCR products had atypical melting curves that did not conform...

journal_title：Journal of clinical microbiology

authors： Anderson TP,Werno AM,Beynon KA,Murdoch DR

更新日期：2003-05-01 00:00:00

abstract：:Studies were conducted to compare and evaluate antimicrobial susceptibility test results for enterococci obtained by six national committee disk diffusion procedures. Variations in the incidence of isolates in resistance categories and errors were associated with the use of ciprofloxacin, gentamicin, nitrofurantoin, r...

journal_title：Journal of clinical microbiology

authors： Cotter G,Adley CC

更新日期：2001-10-01 00:00:00

abstract：:A 45-year-old man from Nepal with a 13-year history of polycystic kidney disease was diagnosed as suffering from chronic renal failure with end-stage renal disease. After receiving empirical antituberculosis treatment, he was treated with broad-spectrum antibiotics. A left nephrectomy was performed, and after 4 months...

journal_title：Journal of clinical microbiology

authors： Padhye AA,Verghese S,Ravichandran P,Balamurugan G,Hall L,Padmaja P,Fernandez MC

更新日期：2003-01-01 00:00:00

abstract：:In a long-term rodent bioassay evaluating the carcinogenicity of triethanolamine, there was equivocal evidence of carcinogenic activity in male B6C3F1 mice, based on a marginal increase in the number of hepatocellular adenomas and hepatoblastomas. Interpretation was complicated by the presence of Helicobacter hepaticu...

journal_title：Journal of clinical microbiology

authors： Fox JG,MacGregor JA,Shen Z,Li X,Lewis R,Dangler CA

更新日期：1998-05-01 00:00:00

abstract：:The p44 gene of the agent of human granulocytic ehrlichiosis (aoHGE) encodes a 44-kDa major outer surface protein. A technique was developed for the typing of the aoHGE based on the PCR amplification of the p44 gene followed by a multiple restriction digest with HindIII, EcoRV, and AspI to generate restriction fragmen...

journal_title：Journal of clinical microbiology

authors： Carter SE,Ravyn MD,Xu Y,Johnson RC

更新日期：2001-09-01 00:00:00

abstract：:Twenty-four isolates of Actinobacillus seminis were typed by PCR ribotyping, repetitive extragenic palindromic element (REP)-based PCR, and enterobacterial repetitive intergenic consensus (ERIC)-based PCR. Five types were distinguished by REP-PCR, and nine types were distinguished by ERIC-PCR. PCR ribotyping produced ...

journal_title：Journal of clinical microbiology

authors： Appuhamy S,Coote JG,Low JC,Parton R

更新日期：1998-03-01 00:00:00

abstract：:Eight commercial enzyme-linked immunosorbent assays (ELISAs) were used to test sera taken from 102 patients in whom Helicobacter pylori infection status had been determined by means of biopsy culture, PCR, histology, and urease production and by 13C urea breath test. By those means, 61 patients had been found to be in...

journal_title：Journal of clinical microbiology

authors： Meijer BC,Thijs JC,Kleibeuker JH,van Zwet AA,Berrelkamp RJ

更新日期：1997-01-01 00:00:00

abstract：:Electrophoretic karyotypes of atypical isolates of Candida albicans, e.g., strains that were germ tube negative, failed to express proteinase activity, demonstrated low virulence for mice, formed hyperchlamydoconidia, produced hyperhyphae, or were sucrose negative (including the type strain of Candida stellatoidea), w...

journal_title：Journal of clinical microbiology

authors： Mahrous M,Lott TJ,Meyer SA,Sawant AD,Ahearn DG

更新日期：1990-05-01 00:00:00