Isolation of nontoxigenic Vibrio cholerae O group 1 from a patient with severe gastrointestinal disease.

abstract：:An indirect fluorescent antibody technique has been developed for the serological diagnosis of gonorrhea. The selected strain(s) of Neisseria gonorrhoeae possesses a heat-labile surface antigen (L-antigen). Sera are diluted 1:10, and an aliquot is hear inactivated at 59 C for 30 min. The treated and untreated aliquots...

journal_title：Journal of clinical microbiology

authors： Gaafar HA,D'Arcangelis DC

更新日期：1976-04-01 00:00:00

abstract：:Herpes infections are among the most common sexually transmitted diseases and are the most common cause of genital ulcer disease in the United States. This study addresses the changing distribution of herpes simplex virus type 1 (HSV-1) and HSV-2 in patients presenting for evaluation of herpetic infections. Viral cult...

journal_title：Journal of clinical microbiology

authors： Ribes JA,Steele AD,Seabolt JP,Baker DJ

更新日期：2001-09-01 00:00:00

abstract：:The recent Zaire Ebola virus (EBOV) outbreak in West Africa illustrates clearly the need for additional studies with humans and animals to elucidate the ecology of Ebola viruses (EBVs). In this study, we developed a serological assay based on the Luminex technology. Nine recombinant proteins representing different vir...

journal_title：Journal of clinical microbiology

authors： Ayouba A,Touré A,Butel C,Keita AK,Binetruy F,Sow MS,Foulongne V,Delaporte E,Peeters M

更新日期：2016-12-28 00:00:00

abstract：:Pathozyme-Myco G (Myco G), M, A, and TB complex plus (Omega Diagnostics Ltd., Alloa, Scotland) were evaluated for the serological diagnosis of pulmonary tuberculosis (TB) in an Argentinean population. Sera from 58 patients with pulmonary TB, 24 subjects with pulmonary mycobacteriosis or mycoses (pulmonary MM group), a...

journal_title：Journal of clinical microbiology

authors： Imaz MS,Comini MA,Zerbini E,Sequeira MD,Latini O,Claus JD,Singh M

更新日期：2004-02-01 00:00:00

abstract：:The hexagonal surface structure believed to be characteristic for influenza C virus was not initially observed for a newly isolated virus of this type. After a few additional egg passages of the virus, however, the hexagonal structure could be readily seen on many virions. ...

journal_title：Journal of clinical microbiology

authors： Martin ML,Palmer EL,Kendal AP

更新日期：1977-07-01 00:00:00

abstract：:While testing the in vitro activities of 14 antimicrobial agents against 107 methicillin-susceptible Staphylococcus aureus (MSSA) and 250 methicillin-resistant S. aureus (MRSA) isolates collected in The Netherlands, we found to our surprise that 19 (7.6%) MRSA isolates were suspected of having reduced susceptibilities...

journal_title：Journal of clinical microbiology

authors： Van Griethuysen A,Van 't Veen A,Buiting A,Walsh T,Kluytmans J

更新日期：2003-06-01 00:00:00

abstract：:The increasing number of multidrug-resistant Mycobacterium tuberculosis strains has stimulated the interest of investigators in finding a rapid method for susceptibility testing. We used commercially available rRNA DNA-bioluminescence-labelled probes (Accu-Probe, Gen Probe, Inc. San Diego, Calif.) for this purpose. Th...

journal_title：Journal of clinical microbiology

authors： Martin-Casabona N,Xairó Mimó D,González T,Rossello J,Arcalis L

更新日期：1997-10-01 00:00:00

abstract：:We undertook a study to evaluate Streck tissue fixative (STF) as a substitute for formalin and polyvinyl alcohol (PVA) in fecal preservation. A comparison of formalin, PVA, (mercuric chloride based), and STF was done by aliquoting fecal samples into each fixative. Stool specimens were collected in Haiti, and parasites...

journal_title：Journal of clinical microbiology

authors： Nace EK,Steurer FJ,Eberhard ML

更新日期：1999-12-01 00:00:00

abstract：:Infections due to biochemically typical Yersinia enterocolitica usually present as gastroenteritis, mesenteric lymphadenitis, terminal ileitis, and septicemia often with visceral abscesses. In these instances, the isolates have been biochemically typical and of well-established serotypes, namely 0:3 or 0:9 and, in the...

journal_title：Journal of clinical microbiology

authors： Bottone EJ

更新日期：1978-06-01 00:00:00

abstract：:Three hundred thirty-one yeast and yeast-like isolates were cultivated on eosin methylene blue agar. While the sensitivity rate for Candida kefyr isolates producing a metallic green sheen was 81.8%, the high positive predictive value (100%) for yeast isolates with this phenotype belonging to C. kefyr suggests that the...

journal_title：Journal of clinical microbiology

authors： Munson EL,Troy DR,Weber JK,Messer SA,Pfaller MA

更新日期：2002-11-01 00:00:00

abstract：:Tracking the spread of noroviruses during outbreaks of gastroenteritis is hampered by the lack of sequence diversity in those regions of the genome chosen for virus detection and characterization. Sequence analysis of regions of the genes encoding the RNA-dependent RNA polymerase and the S domain of the capsid does no...

journal_title：Journal of clinical microbiology

authors： Xerry J,Gallimore CI,Iturriza-Gómara M,Allen DJ,Gray JJ

更新日期：2008-03-01 00:00:00

abstract：:We present the case of a 77-year-old woman who developed an Actinomyces naeslundii infection of a hip prosthesis. The isolate grew well aerobically with 5% CO(2). Possible diagnostic problems may arise in the microbiological laboratory because aerobic growth is not sufficiently accounted for in some of the traditional...

journal_title：Journal of clinical microbiology

authors： Wüst J,Steiger U,Vuong H,Zbinden R

更新日期：2000-02-01 00:00:00

abstract：:A selective agar medium for isolation of virulent Yersinia enterocolitica (VYE agar) was developed for the rapid and accurate isolation of virulent Y. enterocolitica from environmental samples highly contaminated with environmental Yersinia organisms, as well as for isolation from clinical specimens. VYE agar provided...

journal_title：Journal of clinical microbiology

authors： Fukushima H

更新日期：1987-06-01 00:00:00

abstract：:Helicobacter pylori is known to be a major cause of peptic ulceration. The jhp0562 gene, encoding a glycosyltransferase involved in the synthesis of the lipopolysaccharide, was associated with peptic ulcer disease (PUD) in children. The beta-(1,3)-galactosyltransferase [beta-(1,3)GalT] gene (jhp0563), involved in Lewi...

journal_title：Journal of clinical microbiology

authors： Oleastro M,Santos A,Cordeiro R,Nunes B,Mégraud F,Ménard A

更新日期：2010-08-01 00:00:00

abstract：:A diagnostic test for feline infectious peritonitis virus (FIPV) infection based on a nested PCR (nPCR) assay was developed and tested with FIPV, feline enteric coronavirus (FECV), canine coronavirus (CCV), and transmissible gastroenteritis virus (TGEV) and clinical fluid samples from cats with effusive feline infecti...

journal_title：Journal of clinical microbiology

authors： Gamble DA,Lobbiani A,Gramegna M,Moore LE,Colucci G

更新日期：1997-03-01 00:00:00

abstract：:A human astrovirus (HAstV) strain from Kenya was characterized by nucleotide sequence analysis. Sequences from open reading frame 1a (ORF1a) clustered with genotype 6/7, those from ORF1b clustered with genotype 3, and those from ORF2 clustered with genotype 2. A recombination point in the ORF1b-ORF2 junction was ident...

journal_title：Journal of clinical microbiology

authors： Wolfaardt M,Kiulia NM,Mwenda JM,Taylor MB

更新日期：2011-02-01 00:00:00

abstract：:In order to increase the application potential of the direct agglutination test (DAT) for the detection of anti-Leishmania antibodies in human serum samples, we developed an antigen based on stained and freeze-dried Leishmania donovani promastigotes. We describe here the evaluation of the performance of the DAT based ...

journal_title：Journal of clinical microbiology

authors： Meredith SE,Kroon NC,Sondorp E,Seaman J,Goris MG,van Ingen CW,Oosting H,Schoone GJ,Terpstra WJ,Oskam L

更新日期：1995-07-01 00:00:00

abstract：:We describe a Klebsiella oxytoca infection outbreak in a renal transplant unit that involved seven patients. All strains belonged to a single pulsed-field gel electrophoresis pattern and were resistant to amoxicillin-clavulanate, cefuroxime, piperacillin-tazobactam, and aztreonam but susceptible to ceftriaxone, ceftaz...

journal_title：Journal of clinical microbiology

authors： Zárate MS,Gales AC,Picão RC,Pujol GS,Lanza A,Smayevsky J

更新日期：2008-06-01 00:00:00

abstract：:Spiroplasma species are organisms that normally colonize plants and insects. We describe the first case of human systemic infection caused by Spiroplasma bacteria in a patient with hypogammaglobulinemia undergoing treatment with biological disease-modifying antirheumatic agents. Spiroplasma turonicum was identified th...

journal_title：Journal of clinical microbiology

authors： Aquilino A,Masiá M,López P,Galiana AJ,Tovar J,Andrés M,Gutiérrez F

更新日期：2015-02-01 00:00:00

abstract：:Clinical evidence for failure with beta-lactam therapy has been lacking for patients with borderline oxacillin-resistant Staphylococcus aureus (BORSA) infections. We describe a failure of cloxacillin for a patient with endocarditis due to BORSA. The isolate also had false-negative thermonuclease and coagulase test res...

journal_title：Journal of clinical microbiology

authors： Skinner S,Murray M,Walus T,Karlowsky JA

更新日期：2009-03-01 00:00:00

abstract：:Two hundred ninety isolates of Escherichia coli were investigated for the production of extended-spectrum beta-lactamases (ESBLs). Fourteen (4.8%) of the 290 strains were found to produce ESBLs. Each of the 14 strains produced one or two ESBLs, as follows: 10 strains produced TEM-52, 1 strain produced SHV-2a, 1 strain...

journal_title：Journal of clinical microbiology

authors： Pai H,Lyu S,Lee JH,Kim J,Kwon Y,Kim JW,Choe KW

更新日期：1999-06-01 00:00:00

abstract：:We have developed a novel high-throughput PCR-ligase detection reaction-capillary electrophoresis (PCR-LDR-CE) assay for the multiplexed identification of 20 blood-borne pathogens (Staphylococcus epidermidis, Staphylococcus aureus, Bacillus cereus, Enterococcus faecalis, Enterococcus faecium, Listeria monocytogenes, S...

journal_title：Journal of clinical microbiology

authors： Pingle MR,Granger K,Feinberg P,Shatsky R,Sterling B,Rundell M,Spitzer E,Larone D,Golightly L,Barany F

更新日期：2007-06-01 00:00:00

abstract：:Alternative antischistosomal drugs are required since praziquantel is virtually the only drug available for treatment and morbidity control of schistosomiasis. Manual microscopic reading is the current "gold standard" to assess the in vitro antischistosomal properties of test drugs; however, it is labor-intensive, sub...

journal_title：Journal of clinical microbiology

authors： Manneck T,Braissant O,Haggenmüller Y,Keiser J

更新日期：2011-04-01 00:00:00

abstract：:Since the discovery of the Norwalk virus (NV) by immune electron microscopy (IEM) in 1972, serologic studies with this virus have relied on particle-positive fecal material from infected volunteers as the source of antigen because it has not been possible to propagate this virus in cell culture. However, the recent cl...

journal_title：Journal of clinical microbiology

authors： Green KY,Lew JF,Jiang X,Kapikian AZ,Estes MK

更新日期：1993-08-01 00:00:00

abstract：:Twelve strains of Pseudomonas aeruginosa representing 12 serogroups in the serogrouping schema used in the People's Republic of China were compared with serogroups in the International Antigenic Typing System (IATS). The first eight groups originated in the People's Republic of China, and group II appears to have a ne...

journal_title：Journal of clinical microbiology

authors： Liu PV

更新日期：1987-05-01 00:00:00

abstract：:Sequencing-based pathogen identification directly from clinical specimens requires time-consuming interpretation, especially with mixed chromatograms when multiple microorganisms are detected. We assessed RipSeq Mixed software for human papillomavirus (HPV) genotyping by comparison to the linear array HPV genotyping a...

journal_title：Journal of clinical microbiology

authors： Tardif KD,Simmon KE,Kommedal O,Pyne MT,Schlaberg R

更新日期：2013-04-01 00:00:00

abstract：:The new anaerobe and Corynebacterium (ANC) identification card for Vitek 2 was compared with a 16S rRNA gene sequencing (16S) reference method for accuracy in the identification of corynebacteria and anaerobic species. Testing was performed on a Vitek 2 XL system with modified software at three clinical trial laborato...

journal_title：Journal of clinical microbiology

authors： Rennie RP,Brosnikoff C,Turnbull L,Reller LB,Mirrett S,Janda W,Ristow K,Krilcich A

更新日期：2008-08-01 00:00:00

abstract：:Human papillomavirus (HPV) DNA detection and typing are important for diagnosis and management of HPV-associated diseases. One of the most commonly used PCR methods, GP5+/6+, shows weaknesses in amplifying certain types. To circumvent this limitation, we developed and validated broad-spectrum primers targeting the GP5...

journal_title：Journal of clinical microbiology

authors： Schmitt M,Dondog B,Waterboer T,Pawlita M

更新日期：2008-03-01 00:00:00

abstract：:Among 452 samples that were positive by the Xpert MTB/RIF (Xpert) assay and MGIT 960 system (MGIT), 440 and 10 Mycobacterium tuberculosis samples were detected as rifampin susceptible and rifampin resistant, respectively. Two isolates that were rifampin susceptible by the MGIT system were rifampin resistant by the Xpe...

journal_title：Journal of clinical microbiology

authors： Mokaddas E,Ahmad S,Eldeen HS,Al-Mutairi N

更新日期：2015-04-01 00:00:00

abstract：:Due to the inadequate automation in the amplification and sequencing procedures, the use of 16S rRNA gene sequence-based methods in clinical microbiology laboratories is largely limited to identification of strains that are difficult to identify by phenotypic methods. In this study, using conventional full-sequence 16...

journal_title：Journal of clinical microbiology

authors： Woo PC,Ng KH,Lau SK,Yip KT,Fung AM,Leung KW,Tam DM,Que TL,Yuen KY

更新日期：2003-05-01 00:00:00