Isothermal microcalorimetry to study drugs against Schistosoma mansoni.

abstract：:Plasmodium falciparum malaria is highly endemic in the three most affected countries in the current epidemic of Ebola virus disease (EVD) in West Africa. As EVD and malaria are clinically indistinguishable, both remain part of the differential diagnosis of ill travelers from returning from areas of EVD transmission. W...

journal_title：Journal of clinical microbiology

authors： Lau R,Wang A,Chong-Kit A,Ralevski F,Boggild AK

更新日期：2015-04-01 00:00:00

abstract：:A modified CAMP test was used to identify 973 Vibrio cholerae isolates by phenotype. Eltor and non-O1 strains were CAMP positive; classical strains were CAMP negative. Sausage-shaped zones of hemolysis of eltor strains were easily distinguished from narrower bands of non-O1 isolates. For O1 isolates, there was 100% ag...

journal_title：Journal of clinical microbiology

authors： Lesmana M,Subekti D,Tjaniadi P,Pazzaglia G

更新日期：1994-01-01 00:00:00

abstract：:Bacillus anthracis and Bacillus cereus are closely related pathogenic organisms that are difficult to differentiate phenotypically or genotypically. It is well known that vegetative and spore forms of bacilli are quite distinct both morphologically and chemically, but spore-specific chemical markers allowing these spe...

journal_title：Journal of clinical microbiology

authors： Fox A,Black GE,Fox K,Rostovtseva S

更新日期：1993-04-01 00:00:00

abstract：:Hepatitis E virus (HEV) is a major cause of hepatitis. We evaluated five HEV antibody diagnostic assays by using outbreak specimens. The Abbott immunoglobulin G (IgG), Genelabs IgG, and Walter Reed Army Institute of Research (WRAIR) IgM assays were about 90% sensitive; the Abbott IgG and WRAIR total Ig and IgM assays ...

journal_title：Journal of clinical microbiology

authors： Myint KS,Endy TP,Gibbons RV,Laras K,Mammen MP Jr,Sedyaningsih ER,Seriwatana J,Glass JS,Narupiti S,Corwin AL

更新日期：2006-04-01 00:00:00

abstract：:A monoclonal antibody specific for group D Salmonella antigen 9 was used in an indirect enzyme-linked immunosorbent assay (ELISA) for detecting the antigen in urine specimens collected from patients with clinical typhoid fever in Jakarta, Indonesia. The ELISA had a sensitivity of 95% in identifying patients in whom Sa...

journal_title：Journal of clinical microbiology

authors： Chaicumpa W,Ruangkunaporn Y,Burr D,Chongsa-Nguan M,Echeverria P

更新日期：1992-09-01 00:00:00

abstract：:Prototheca spp. are environmental algae that may cause serious infection in the immunocompromised patient. Clinical manifestations may mimic other diseases. We present a case of fatal infection in a 78-year-old cardiac transplant recipient and discuss pitfalls in the clinical and laboratory diagnoses. ...

journal_title：Journal of clinical microbiology

authors： McMullan B,Muthiah K,Stark D,Lee L,Marriott D

更新日期：2011-08-01 00:00:00

abstract：:This study evaluated a PCR method for the rapid detection of clinically significant genotypes of vancomycin-resistant enterococci (VRE) in nosocomial surveillance specimens. Detection of the vanA and vanB genes by multiplex PCR using 657 specimens that showed presumptive growth of VRE on bile esculin azide agar contai...

journal_title：Journal of clinical microbiology

authors： Jayaratne P,Rutherford C

更新日期：1999-06-01 00:00:00

abstract：:Bovine vaginal cytobrush specimens were analyzed for the presence of Chlamydia spp. by a high-sensitivity, high-specificity quantitative PCR. The 53% prevalence of low-level Chlamydia psittaci and C. pecorum genital infection detected in virgin heifers suggests predominantely extragenital transmission of Chlamydia in ...

journal_title：Journal of clinical microbiology

authors： DeGraves FJ,Gao D,Hehnen HR,Schlapp T,Kaltenboeck B

更新日期：2003-04-01 00:00:00

abstract：:Clostridioides (Clostridium) difficile infection (CDI) is the most common causative pathogen of health care-associated gastrointestinal infections; however, due to the overlap of clinical symptoms with those of other causes of acute gastroenteritis, the selection of the most appropriate laboratory test is difficult. F...

journal_title：Journal of clinical microbiology

authors： Krutova M,Briksi A,Tkadlec J,Zajac M,Matejkova J,Nyc O,Drevinek P

更新日期：2019-09-24 00:00:00

abstract：:The Analytab Products, Inc. (API), anaerobic multitest microsystem (MICRO) was compared with the Center for Disease Control conventional (CONV) thioglycolate (supplemented with hemin and vitamin K1) system and with pre-reduced anaerobically sterilized (PRAS) media as recommended by the Virginia Polytechnic Institute. ...

journal_title：Journal of clinical microbiology

authors： Moore HB,Sutter VL,Finegold SM

更新日期：1975-01-01 00:00:00

abstract：:The extent and importance of genotype heterogeneity of Pneumocystis carinii f. sp. hominis within lungs have not previously been investigated. Two hundred forty PCR clones obtained from respiratory specimens and lung segments from three patients with fatal P. carinii pneumonia were investigated to detect genetic diver...

journal_title：Journal of clinical microbiology

authors： Helweg-Larsen J,Lundgren B,Lundgren JD

更新日期：2001-10-01 00:00:00

abstract：:Antibiogram typing of methicillin-resistant Staphylococcus aureus with selected antibiotics was evaluated as a primary epidemiological typing tool and compared with ribotyping. Antibiograms were derived with the Kirby-Bauer disk diffusion method by using erythromycin, clindamycin, cotrimoxazole, gentamicin, and ciprof...

journal_title：Journal of clinical microbiology

authors： Blanc DS,Lugeon C,Wenger A,Siegrist HH,Francioli P

更新日期：1994-10-01 00:00:00

abstract：:We report on a reverse line blot (RLB) assay, utilizing fungal species-specific oligonucleotide probes to hybridize with internal transcribed spacer 2 region sequences amplified using a nested panfungal PCR. Reference and clinical strains of 16 Candida species (116 strains), Cryptococcus neoformans (five strains of Cr...

journal_title：Journal of clinical microbiology

authors： Playford EG,Kong F,Sun Y,Wang H,Halliday C,Sorrell TC

更新日期：2006-03-01 00:00:00

abstract：:A toxic shock syndrome toxin (TSST) variant with an isoelectric point (pI) of 8.6 produced by an ovine-associated Staphylococcus aureus strain was described previously. Analysis of additional strains associated with sheep, goats, cows, and humans by isoelectric focusing with immunoblotting using monoclonal antibodies ...

journal_title：Journal of clinical microbiology

authors： Ho G,Campbell WH,Bergdoll MS,Carlson E

更新日期：1989-09-01 00:00:00

abstract：:A real-time quantitative PCR assay has been developed to measure human herpesvirus 6 (HHV-6) DNA in biological specimens. The assay sensitivity was 10 copies of DNA per well, with a linear dynamic range of 10 to 10(7) copies of HHV-6 DNA. Intra- and interassay variations were, respectively, 0.88 and 0.8% for samples c...

journal_title：Journal of clinical microbiology

authors： Collot S,Petit B,Bordessoule D,Alain S,Touati M,Denis F,Ranger-Rogez S

更新日期：2002-07-01 00:00:00

abstract：:Bacteriological and morphological studies of 38 brown pigment common duct stones were performed. Stone cultures were positive for bacteria in 80.5% of those studied. Enterococci were the most common organisms that were isolated. Scanning electron microscopy showed the presence of bacteria in 84.2% of the stones. The b...

journal_title：Journal of clinical microbiology

authors： Leung JW,Sung JY,Costerton JW

更新日期：1989-05-01 00:00:00

abstract：:Actinomyces naeslundii is a saprophyte, sometimes a pathogen, of the human oral cavity. Very few extra-oral infections related to this agent have been described. We report the first instance of A. naeslundii as an etiological agent of pelvic actinomycosis in a user of an intrauterine device, an infection so far exclus...

journal_title：Journal of clinical microbiology

authors： Bonnez W,Lattimer G,Mohanraj NA,Johnson TH

更新日期：1985-02-01 00:00:00

abstract：:We have shown that a modification of the color-coded antigen developed by Kasatiya and Lambert (Appl. Microbiol. 28:317-318, 1974) can be used as a substitute for other nontreponemal antigens used in screening tests for syphilis. The antigen is based on the Venereal Disease Research Laboratory antigen, with EDTA, chol...

journal_title：Journal of clinical microbiology

authors： Pettit DE,Larsen SA,Harbec PS,Feeley JC,Parham CE,Cruce DD,Hambie EA,Perryman MW

更新日期：1983-11-01 00:00:00

abstract：:Hepatitis E virus (HEV) is responsible for a majority of sporadic and epidemic viral hepatitides in India and other developing countries. Even though the genomes of four geographically distinct strains of HEV have been cloned and sequenced, the Indian strain of HEV remains largely uncharacterized. We have cloned and s...

journal_title：Journal of clinical microbiology

authors： Panda SK,Nanda SK,Zafrullah M,Ansari IH,Ozdener MH,Jameel S

更新日期：1995-10-01 00:00:00

abstract：:The bacterial genus Gordona includes seven species of mycolic acid-containing actinomycetes which are cultured from environmental sources and rarely from clinical samples. They have been implicated in primary pulmonary diseases, nosocomial wound infections, and central nervous system infections in two immunocompromise...

journal_title：Journal of clinical microbiology

authors： Drancourt M,Pelletier J,Cherif AA,Raoult D

更新日期：1997-02-01 00:00:00

abstract：:Testing efforts for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been burdened by the scarcity of testing materials and personal protective equipment for health care workers. The simple and painless process of saliva collection allows for widespread testing, but enthusiasm is hampered by variable ...

journal_title：Journal of clinical microbiology

authors： Yee R,Truong TT,Pannaraj PS,Eubanks N,Gai E,Jumarang J,Turner L,Peralta A,Lee Y,Dien Bard J

更新日期：2021-01-21 00:00:00

abstract：:Two multiplex PCRs were developed for the detection of necrotoxigenic Escherichia coli virulence genes. M1 contained the primers for the toxins and the aerobactin, and M2 contained the primers for the adhesins. They were validated by single PCRs performed with reference E. coli strains and by multiplex PCRs with necro...

journal_title：Journal of clinical microbiology

authors： Van Bost S,Jacquemin E,Oswald E,Mainil J

更新日期：2003-09-01 00:00:00

abstract：:The purpose of this study was to evaluate the performance of a herpes simplex virus (HSV) type 1-specific anti-glycoprotein C-1 monoclonal antibody (MAb) and a type 2-specific anti-glycoprotein G-2 MAb for typing of 2,400 clinical HSV-1 isolates and 2,400 clinical HSV-2 isolates, respectively, using an enzyme immunoas...

journal_title：Journal of clinical microbiology

authors： Liljeqvist JA,Svennerholm B,Bergström T

更新日期：1999-08-01 00:00:00

abstract：:Nucleic acid tests are sensitive and specific and provide a rapid diagnosis, making them invaluable for patient and outbreak management. Multiplex PCR assays have additional advantages in providing an economical and comprehensive panel for many common respiratory viruses. Previous reports have shown the utility of the...

journal_title：Journal of clinical microbiology

authors： Pabbaraju K,Wong S,Tokaryk KL,Fonseca K,Drews SJ

更新日期：2011-05-01 00:00:00

abstract：:For recovery of brucellae from clinical specimens, a lysis-concentration procedure was superior to the procedure of Castañeda (M. R. Castañeda, Proc. Soc. Exp. Biol. Med. 64:114-115, 1947). ...

journal_title：Journal of clinical microbiology

authors： Etemadi H,Raissadat A,Pickett MJ,Zafari Y,Vahedifar P

更新日期：1984-09-01 00:00:00

abstract：:Hepatitis G virus (HGV) was recently identified as a new member of the Flaviviridae, but its clinical significance is still unclear. Since no immunoassay for the diagnosis of HGV is available, we developed a sensitive reverse transcription-PCR (RT-PCR) assay to facilitate the detection of the viral genome by mass scre...

journal_title：Journal of clinical microbiology

authors： Schlueter V,Schmolke S,Stark K,Hess G,Ofenloch-Haehnle B,Engel AM

更新日期：1996-11-01 00:00:00

abstract：:A biotin-avidin-amplified indirect immunofluorescence method was used to demonstrate specific serum immunoglobulin M (IgM) antibodies in nephropathia epidemica, the Scandinavian type of hemorrhagic fever with renal syndrome. The antigen in the test was the cross-reacting agent of Korean hemorrhagic fever, Hantaan viru...

journal_title：Journal of clinical microbiology

authors： Settergren B,Juto P,Wadell G

更新日期：1987-06-01 00:00:00

abstract：:PCR-amplified 16S rRNA gene sequences were obtained directly from tissue specimens from eight cats with presumptive feline leprosy. Acid-fast bacilli were observed in sections from all eight specimens, but culture for mycobacteria was successful for one specimen only. Analysis of the V2 variable region of each 16S rRN...

journal_title：Journal of clinical microbiology

authors： Hughes MS,Ball NW,Beck LA,de Lisle GW,Skuce RA,Neill SD

更新日期：1997-10-01 00:00:00

abstract：:A specific PCR for the detection of a variant of the gene encoding Shiga toxin 1 (stx(1)) called stx(1(OX3)) (GenBank accession no. Z36901) was developed. The PCR was used to investigate 148 Stx(1)-producing Escherichia coli strains from human patients (n = 72), cattle (n = 27), sheep (n = 48), and a goat (n = 1) for ...

journal_title：Journal of clinical microbiology

authors： Koch C,Hertwig S,Lurz R,Appel B,Beutin L

更新日期：2001-11-01 00:00:00

abstract：:Of 12,321 stool samples analyzed over a 6-year interval, 870 (7.1%) were positive for a total of 1,019 parasites, of which 1,011 (99.2%) were found in trichrome-stained smears of unconcentrated specimens while only 479 (47.0%) were detected in iodine-stained smears of concentrated samples. Stool specimens were next an...

journal_title：Journal of clinical microbiology

authors： Kellogg JA,Elder CJ

更新日期：1999-03-01 00:00:00