Abstract：
:The increasing number of multidrug-resistant Mycobacterium tuberculosis strains has stimulated the interest of investigators in finding a rapid method for susceptibility testing. We used commercially available rRNA DNA-bioluminescence-labelled probes (Accu-Probe, Gen Probe, Inc. San Diego, Calif.) for this purpose. The study was performed in three chronological steps. (i) We studied the correlation between the photometric light units (PLUs) given by the hybridization method, the numbers of CFU per milliliter, and turbidity as nephelometric units for six different inocula of an M. tuberculosis strain over 14 days. A good correlation (c > 0.9; P < 0.05) was found from the third day for all concentrations used. (ii) Over a period of 14 days we studied the evolution of the PLUs for 20 strains growing in medium with 0.2 microl of isoniazid (H) per ml and 18 strains in medium with 1 microl of rifampin (R) per ml to standardize the method. Susceptible and resistant strains were used according to the reference proportions method in Middlebrook 7H10, and the MICs were determined in solid and liquid media. The final inoculum of a 10(-2) dilution from a McFarland no. 1 standard and reading at 3 and 5 days provided the best results. A quotient was established to find a cutoff point between resistant and susceptible strains. (iii) We used the standardized parameters in 117 tests with H and R. On day 3, the sensitivity, specificity, positive predictive value, and negative predictive value for detecting resistant strains were 86.8, 100, 100, and 90.1%, respectively, and on day 5 they were 96.2, 100, 100, and 94%, respectively. We concluded that the method is readily available, is easy to perform, and could be useful for screening resistant M. tuberculosis strains.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Martin-Casabona N,Xairó Mimó D,González T,Rossello J,Arcalis Ldoi
10.1128/JCM.35.10.2521-2525.1997subject
Has Abstractpub_date
1997-10-01 00:00:00pages
2521-5issue
10eissn
0095-1137issn
1098-660Xjournal_volume
35pub_type
杂志文章abstract：:Ninety-one isolates of nutritionally variant streptococci (NVS) that were previously isolated from the human mouth were regarded as consisting of 7 Streptococcus defectivus isolates, 78 Streptococcus adjacens isolates, and 6 Gemella morbillorum isolates. However, recent references to the taxonomic reclassification of ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.37.8.2553-2556.1999
更新日期：1999-08-01 00:00:00
abstract：:PCR of a Candida albicans cytochrome P-450 lanosterol-alpha-demethylase (P450-L1A1) gene segment is a rapid and sensitive method of detection in clinical specimens. This enzyme is a target for azole antifungal action. In order to directly detect and identify the clinically most important species of Candida, we cloned ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.32.8.1902-1907.1994
更新日期：1994-08-01 00:00:00
abstract：:In this issue of the Journal of Clinical Microbiology, R. E. Bumgarner, D. Harrison, and J. E. Hsu (J Clin Microbiol 58:e00121-19, 2020, https://doi.org/10.1128/JCM.00121-19) address in a retrospective analysis that clonality of Cutibacterium acnes isolates from deep tissue specimens obtained from patients during revi...
journal_title：Journal of clinical microbiology
pub_type： 评论,杂志文章
doi：10.1128/JCM.01638-19
更新日期：2020-01-28 00:00:00
abstract：:Indirect hemagglutinating and immunofluorescent antibody responses to Herpesvirus hominis types 1 and 2 were compared to neutralizing antibody responses in infected humans from whom H. hominis type 1 or 2 was isolated. The indirect immunofluorescent antibody test was shown to be the most sensitive and specific for pri...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1979-03-01 00:00:00
abstract：:A neutralizing monoclonal antibody, B37:1, against a human rotavirus (B37) with a "super-short" RNA pattern was derived. The antibody gave serotype-specific reactions with a panel of standard rotaviruses and was reactive in an enzyme immunoassay. Epidemiological studies of this new human serotype should now be possibl...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.25.12.2426-2427.1987
更新日期：1987-12-01 00:00:00
abstract：:mRNAs of human papillomaviruses (HPV) 16 and 18 were detected in cancer-derived cell lines and genital tract biopsy specimens by a novel hybridization assay. Biotinylated whole genomic HPV DNA probes were hybridized in solution to extracted total nucleic acids. Hybrids between the labeled probes and RNA transcripts we...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.29.5.968-974.1991
更新日期：1991-05-01 00:00:00
abstract：:This three-center collaborative study was conducted to evaluate samples of the Microbial Profile System (MPS) antimicrobial microdilution panels [previously produced by Minnesota Mining & Manufacturing Co., (3M Co.), St. Paul, Minn. and currently produced by Flow Laboratories, Inc., Rockville, Md.). This was a three-p...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.17.3.436-444.1983
更新日期：1983-03-01 00:00:00
abstract：:The first outbreak caused by vancomycin-resistant enterococci of the VanB phenotype in Poland was analyzed. It occurred in a single ward of a Warsaw hospital which is a specialized center for the treatment of hematological disorders. Between July 1999 and February 2000, 11 patients in the ward were found to be infecte...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.39.5.1781-1787.2001
更新日期：2001-05-01 00:00:00
abstract：:Typing of Mycobacterium avium subspecies paratuberculosis strains presents a challenge, since they are genetically monomorphic and traditional molecular techniques have limited discriminatory power. The recent advances and availability of whole-genome sequencing have extended possibilities for the characterization of ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.01958-15
更新日期：2016-03-01 00:00:00
abstract：:This paper describes the first isolation of a new Shigella flexneri serotype, designated 4s, in Beijing, China. Genotypic and phenotypic profiling suggests that this isolate is a clone of the S. flexneri serotype X variant reference strain. Of particular concern is the multidrug resistance exhibited by this isolate. ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.01946-10
更新日期：2011-03-01 00:00:00
abstract：:In a prospective observational study of bacteremic patients we ascertained the influence of different parts of culture results on the correctness of empirical antibiotic therapy. Ninety-three bacteremic patients requiring antibiotic treatment were included. Patients who had consultations with an infectious disease con...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.06051-11
更新日期：2012-06-01 00:00:00
abstract：:In order to determine whether mixed infections of human herpesvirus 6B (HHV-6B) occur in immunocompetent and immunocompromised individuals, we examined the copy numbers of telomeric repeat sequences (TRS) of clinical isolates. In clinical isolates obtained from patients with exanthem subitum caused by primary HHV-6B i...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.02192-13
更新日期：2014-02-01 00:00:00
abstract：:Counterimmunoelectrophoresis was compared with electron microscopy for detection of human reovirus-like agent in fecal specimens. Both tests gave very similar results. ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1977-02-01 00:00:00
abstract：:We compared 75 nontypeable (NT) Haemophilus influenzae isolates by pulsed-field gel electrophoresis (PFGE), enterobacterial repetitive intergenic consensus (ERIC)-PCR, and automated ribotyping. PFGE was the most discriminatory of the techniques. ERIC-PCR provides a useful screen but should not replace other techniques...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/jcm.40.2.660-662.2002
更新日期：2002-02-01 00:00:00
abstract：:During the course of a molecular epidemiology study of mechanisms of antibiotic resistance in the area served by our hospital (516,000 inhabitants), we isolated the gene encoding CTX-M-14 beta-lactamase. Thirty clinical strains (27 Escherichia coli and 3 Klebsiella pneumoniae isolates) with a phenotype of extended-spe...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/jcm.40.11.4030-4036.2002
更新日期：2002-11-01 00:00:00
abstract：:Molecular genotyping of strains of Trichophyton rubrum and T. mentagrophytes from patients with onychomycosis of the toes was performed to ascertain whether the fungal genotype changes over the course of time as sequential samples were obtained from patients receiving antifungal therapy and during follow-up. Sixty-six...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/jcm.39.9.3260-3266.2001
更新日期：2001-09-01 00:00:00
abstract：:A transrectal prostate biopsy is the most common procedure used to establish the diagnosis of prostate cancer. Prior to biopsy, patients are commonly given ciprofloxacin for prophylaxis. However, a complication of the procedure is infection with ciprofloxacin-resistant organisms, in particular resistant Escherichia co...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.02158-12
更新日期：2013-01-01 00:00:00
abstract：:Adolescent children were surveyed for colonization with Mycoplasma hominis and Ureaplasma urealyticum by culturing urine specimens. Rates were compared between three study groups: (i) 397 children attending parochial schools, (ii) 293 children attending an adolescent clinic specializing in adjustment problems, and (ii...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1975-09-01 00:00:00
abstract：:During an investigation of a hospital outbreak of norovirus gastroenteritis identified as being caused by a recombinant genogroup II (rGII-3a) strain, fecal specimens collected from asymptomatic staff and patients were tested by nested PCR. A GII-4 norovirus strain, the predominant strain associated with outbreaks in ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/jcm.42.5.2271-2274.2004
更新日期：2004-05-01 00:00:00
abstract：:Vibrio cholerae serotype O1 organisms that do not produce cholera toxin and, in fact, lack the genetic material encoding the enterotoxin have recently been detected in coastal regions of the United States. Although these organisms have been assumed to be nonpathogenic, they have been considered a potential reservoir o...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.17.5.918-920.1983
更新日期：1983-05-01 00:00:00
abstract：:Alternative antischistosomal drugs are required since praziquantel is virtually the only drug available for treatment and morbidity control of schistosomiasis. Manual microscopic reading is the current "gold standard" to assess the in vitro antischistosomal properties of test drugs; however, it is labor-intensive, sub...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.02382-10
更新日期：2011-04-01 00:00:00
abstract：:Time to detection (TTD) in automated blood culture systems is delayed for sensitive microorganisms in the presence of antimicrobial substances and has been associated with worse outcomes for sepsis patients on inadequate empirical therapy. While resin addition removes antimicrobial substances to various degrees from b...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.00103-13
更新日期：2013-05-01 00:00:00
abstract：:A commercially available endotoxin assay (CS-TAL) employing a chromogenic peptide and an amebocyte lysate from the Japanese horseshoe crab, Tachypleus tridentatus, gave a positive result with aqueous extracts of all 15 strains of Candida albicans and 1 strain each of Candida tropicalis, Cryptococcus neoformans, and a ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.25.9.1701-1704.1987
更新日期：1987-09-01 00:00:00
abstract：:The DNA hybridization assay for genes encoding for Escherichia coli enterotoxins was used to examine water specimens in Thailand. In a reconstruction experiment, the DNA hybridization assay was 10(4) times more sensitive than testing random E. coli in the Y-1 adrenal and suckling mouse assays in identifying enterotoxi...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.16.6.1086-1090.1982
更新日期：1982-12-01 00:00:00
abstract：:With use of the rabbit intestinal loop model, heat-labile enterotoxins from 21 Escherichia coli strains isolated from a wide spectrum of patients with diarrheal diseases were all neutralized to high titer by two antisera prepared against enterotoxins of either E. coli or Vibrio cholerae. These findings suggest marked ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1977-06-01 00:00:00
abstract：:The COVID-19 pandemic has put the clinical laboratory in the spotlight. The news media is regularly seeking out interviews with microbiologists, infectious disease specialists, and pathologists. Increased public exposure offers opportunities to improve how laboratory professionals communicate our insights. We can emph...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.02917-20
更新日期：2021-01-21 00:00:00
abstract：:Sixty-six Corynebacterium diphtheriae strains (62 of the gravis biotype and 4 of the mitis biotype) isolated during the Georgian diphtheria epidemic of 1993 to 1998 and 13 non-Georgian C. diphtheriae strains (10 Russian and 3 reference isolates) were characterized by (i) biotyping, (ii) toxigenicity testing with the E...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.37.10.3265-3270.1999
更新日期：1999-10-01 00:00:00
abstract：:Commercially available latex agglutination and coagglutination reagents were evaluated for their ability to detect bacterial antigens in the sera of 165 patients to determine their suitability for rapid diagnosis of pneumonia. These reagents were used to detect the polysaccharide capsular antigens of Haemophilus influ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.25.2.248-250.1987
更新日期：1987-02-01 00:00:00
abstract：:We have evaluated the diagnostic utility of six antigenic regions of the Toxoplasma gondii MIC2, MIC3, M2AP, GRA3, GRA7, and SAG1 gene products, assembled in recombinant chimeric antigens by genetic engineering, in order to replace the soluble, whole-cell tachyzoite extract in serological assays. Serum samples from 10...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.00237-06
更新日期：2006-06-01 00:00:00
abstract：:The aim of this study was to find a reliable method for the detection and identification of fungi in fungus balls of the maxillary sinus and to evaluate the spectrum of fungi in these samples. One hundred twelve samples were obtained from patients with histologically proven fungal infections; 81 samples were paraffin-...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/jcm.41.2.581-585.2003
更新日期：2003-02-01 00:00:00
