Comparison of indirect hemagglutination and indirect immunofluorescence tests with microneutralization tests for detection of type-specific Herpesvirus hominis antibody.

abstract：:Ninety-one isolates of nutritionally variant streptococci (NVS) that were previously isolated from the human mouth were regarded as consisting of 7 Streptococcus defectivus isolates, 78 Streptococcus adjacens isolates, and 6 Gemella morbillorum isolates. However, recent references to the taxonomic reclassification of ...

journal_title：Journal of clinical microbiology

authors： Sato S,Kanamoto T,Inoue M

更新日期：1999-08-01 00:00:00

abstract：:The 16S rRNA gene PCR in the diagnosis of bone and joint infections has not been systematically tested. Five hundred twenty-five bone and joint samples collected from 525 patients were cultured and submitted to 16S rRNA gene PCR detection of bacteria in parallel. The amplicons with mixed sequences were also cloned. Wh...

journal_title：Journal of clinical microbiology

authors： Fenollar F,Roux V,Stein A,Drancourt M,Raoult D

更新日期：2006-03-01 00:00:00

abstract：:The present investigation focused on genetic diversity and drug resistance of 101 Mycobacterium tuberculosis strains isolated between July 2003 and February 2005 in the Okinawa prefecture, Ryukyu Islands, Japan. A high rate of clustering (87%, eight clusters, 2 to 69 strains/cluster) was observed upon spoligotyping; m...

journal_title：Journal of clinical microbiology

authors： Millet J,Miyagi-Shiohira C,Yamane N,Sola C,Rastogi N

更新日期：2007-11-01 00:00:00

abstract：:Methicillin-resistant Staphylococcus aureus (MRSA) of sequence type 398 (ST398) has frequently been detected in pigs and pig handlers. However, in Malaysia, sampling 360 pigs and 90 pig handlers from 30 farms identified novel ST9-spa type t4358-staphylococcal cassette chromosome mec type V MRSA strains that were found...

journal_title：Journal of clinical microbiology

authors： Neela V,Mohd Zafrul A,Mariana NS,van Belkum A,Liew YK,Rad EG

更新日期：2009-12-01 00:00:00

abstract：:The p44 gene of the agent of human granulocytic ehrlichiosis (aoHGE) encodes a 44-kDa major outer surface protein. A technique was developed for the typing of the aoHGE based on the PCR amplification of the p44 gene followed by a multiple restriction digest with HindIII, EcoRV, and AspI to generate restriction fragmen...

journal_title：Journal of clinical microbiology

authors： Carter SE,Ravyn MD,Xu Y,Johnson RC

更新日期：2001-09-01 00:00:00

abstract：:The rapid ID32A kit (bioMérieux Vitek, Inc., Hazelwood, Mo.) was evaluated for its ability to identify Peptostreptococcus species compared with conventional biochemical tests and gas-liquid chromatography (Virginia Polytechnic Institute), the current "gold standard" method. A total of 5 Peptostreptococcus American Typ...

journal_title：Journal of clinical microbiology

authors： Ng J,Ng LK,Chow AW,Dillon JA

更新日期：1994-05-01 00:00:00

abstract：:In this study, we evaluated a rapid whole-blood test, BM-test Helicobacter pylori, for detection of H. pylori infection in 144 and 48 patients with other gastrointestinal symptoms and with gastric cancer, respectively. The sensitivity, specificity, positive predictive value, negative predictive value, and diagnostic a...

journal_title：Journal of clinical microbiology

authors： Enroth H,Rigo R,Hultén K,Engstrand L

更新日期：1997-10-01 00:00:00

abstract：:The human pathogen Staphylococcus aureus is isolated and characterized using traditional culture and sensitivity methodologies that are slow and offer limited information on the organism. In contrast, DNA microarray technology can provide detailed, clinically relevant information on the isolate by detecting the presen...

journal_title：Journal of clinical microbiology

authors： Spence RP,Wright V,Ala-Aldeen DA,Turner DP,Wooldridge KG,James R

更新日期：2008-05-01 00:00:00

abstract：:We examined several factors for their effects on the serological reactivity of Legionella antigens used for direct or indirect fluorescent-antibody tests. These factors included media, methods of killing, strain differences, and the nature of the reactivity with diverse human sera. The maximum serological reactivities...

journal_title：Journal of clinical microbiology

authors： Benson RF,Malcolm GB,Pine L,Harrell WK

更新日期：1983-05-01 00:00:00

abstract：:A panel (ENVA-1) of well-defined blinded samples containing wild-type and mutant human immunodeficiency virus type 1 (HIV-1) reverse transcriptase was analyzed by automated DNA sequencing in 23 laboratories worldwide. Drug resistance mutations at codons 41, 215, and 184 were present in the panel samples at different r...

journal_title：Journal of clinical microbiology

authors： Schuurman R,Demeter L,Reichelderfer P,Tijnagel J,de Groot T,Boucher C

更新日期：1999-07-01 00:00:00

abstract：:The commercially available diagnostic tests for syphilis are mostly based on the use of extracted antigens of Treponema pallidum. Pronounced cross-reactivities with other spirochete antigens are often reported. The aim of this study was to validate a novel multiparametric assay (the assay performed with the kit) INNO-...

journal_title：Journal of clinical microbiology

authors： Ebel A,Vanneste L,Cardinaels M,Sablon E,Samson I,De Bosschere K,Hulstaert F,Zrein M

更新日期：2000-01-01 00:00:00

abstract：:Helicobacter pylori is known to be a major cause of peptic ulceration. The jhp0562 gene, encoding a glycosyltransferase involved in the synthesis of the lipopolysaccharide, was associated with peptic ulcer disease (PUD) in children. The beta-(1,3)-galactosyltransferase [beta-(1,3)GalT] gene (jhp0563), involved in Lewi...

journal_title：Journal of clinical microbiology

authors： Oleastro M,Santos A,Cordeiro R,Nunes B,Mégraud F,Ménard A

更新日期：2010-08-01 00:00:00

abstract：:We attempted to find a relationship between the microbiological properties of bloodstream isolates of methicillin-resistant Staphylococcus aureus (MRSA) and the efficacy of vancomycin in the treatment of bacteremia. Vancomycin susceptibility testing was performed, and bactericidal activity was determined for 30 isolat...

journal_title：Journal of clinical microbiology

authors： Sakoulas G,Moise-Broder PA,Schentag J,Forrest A,Moellering RC Jr,Eliopoulos GM

更新日期：2004-06-01 00:00:00

abstract：:Nineteen Rhizomucor miehei and Rhizomucor pusillus isolates were assayed for their ability to utilize 87 various substrates as a single carbon source. Besides a difference in sucrose utilization, distinctive differences were found in the utilization of glycine, phenylalanine, and beta-alanine. Five isoenzyme systems a...

journal_title：Journal of clinical microbiology

authors： Vastag M,Papp T,Kasza Z,Vágvölgyi C

更新日期：1998-07-01 00:00:00

abstract：:In 1991, multiresistant Escherichia coli O78:H10 strains caused an outbreak of urinary tract infections in Copenhagen, Denmark. The phylogenetic origin, clonal background, and virulence characteristics of the outbreak isolates, and their relationship to nonoutbreak O78:H10 strains according to these traits and resista...

journal_title：Journal of clinical microbiology

authors： Olesen B,Scheutz F,Andersen RL,Menard M,Boisen N,Johnston B,Hansen DS,Krogfelt KA,Nataro JP,Johnson JR

更新日期：2012-11-01 00:00:00

abstract：:We have evaluated the KOH test, the antibiotic disk identification test, and the Gram stain reaction for the preliminary grouping of gram-positive and gram-negative anaerobes and have assessed the value of erythromycin 60-micrograms-disk resistance as a predictive index of clindamycin resistance among anaerobes. By te...

journal_title：Journal of clinical microbiology

authors： Bourgault AM,Lamothe F

更新日期：1988-10-01 00:00:00

abstract：:The TRUGENE HIV-1 Genotyping Kit and OpenGene DNA Sequencing System are designed to sequence the protease (PR)- and reverse transcriptase (RT)-coding regions of human immunodeficiency virus type 1 (HIV-1) pol. Studies were undertaken to determine the accuracy of this assay system in detecting resistance-associated mut...

journal_title：Journal of clinical microbiology

authors： Kuritzkes DR,Grant RM,Feorino P,Griswold M,Hoover M,Young R,Day S,Lloyd RM Jr,Reid C,Morgan GF,Winslow DL

更新日期：2003-04-01 00:00:00

abstract：:The nucleic acid sequence of the putative 5'-untranslated (5PUT) region of hepatitis C virus (HCV), determined for samples obtained from a variety of geographic origins, was found to be over 98% conserved among all isolates. On the basis of this signature sequence for HCV, a viral RNA assay was developed by using cDNA...

journal_title：Journal of clinical microbiology

authors： Cha TA,Kolberg J,Irvine B,Stempien M,Beall E,Yano M,Choo QL,Houghton M,Kuo G,Han JH

更新日期：1991-11-01 00:00:00

abstract：:Forty-four nonreplicate clinical isolates of Pseudomonas aeruginosa that were resistant to extended-spectrum cephalosporins (ceftazidime and cefepime) and aztreonam, that putatively produced an acquired extended- spectrum beta-lactamase (ESBL), according to the results of a double-disk synergy test, and that had been ...

journal_title：Journal of clinical microbiology

authors： Pagani L,Mantengoli E,Migliavacca R,Nucleo E,Pollini S,Spalla M,Daturi R,Romero E,Rossolini GM

更新日期：2004-06-01 00:00:00

abstract：:Blood specimens collected from adult patients with suspected sepsis in four medical centers were inoculated into BACTEC Plus/F and BacT/Alert FAN aerobic culture bottles. Both bottles of 7,401 bottle pairs contained the prescribed blood volume of 8 to 12 ml. Bottles were incubated in their respective instruments for a...

journal_title：Journal of clinical microbiology

authors： Jorgensen JH,Mirrett S,McDonald LC,Murray PR,Weinstein MP,Fune J,Trippy CW,Masterson M,Reller LB

更新日期：1997-01-01 00:00:00

abstract：:Because self-collected vaginal swabs (VS) are potentially very useful for screening asymptomatic women for Chlamydia trachomatis infection, a multicenter study evaluated that specimen with nucleic acid amplification tests (NAATs). The objective was to determine whether VS are equal to Food and Drug Administration (FDA...

journal_title：Journal of clinical microbiology

authors： Schachter J,McCormack WM,Chernesky MA,Martin DH,Van Der Pol B,Rice PA,Hook EW 3rd,Stamm WE,Quinn TC,Chow JM

更新日期：2003-08-01 00:00:00

abstract：:The fungus Fusarium sacchari was isolated repeatedly from the blood of an immunosuppressed host. The infection was treated successfully with a small dose of amphotericin B. The strain was resistant to this antifungal in vitro. MICs and minimum fungicidal concentrations of six antifungals for the clinical isolate are p...

journal_title：Journal of clinical microbiology

authors： Guarro J,Nucci M,Akiti T,Gené J,Barreiro MD,Gonçalves RT

更新日期：2000-01-01 00:00:00

abstract：:Twenty-three nasal swab samples that tested positive for methicillin-resistant Staphylococcus aureus (MRSA) on initial testing by the BD GeneOhm MRSA assay (BD-MRSA PCR; BD GeneOhm, San Diego, CA) were culture positive only for methicillin-susceptible S. aureus (MSSA) from an enrichment broth. The 23 recovered isolate...

journal_title：Journal of clinical microbiology

authors： Stamper PD,Louie L,Wong H,Simor AE,Farley JE,Carroll KC

更新日期：2011-04-01 00:00:00

abstract：:Staphylococcus aureus produced an inhibitory factor that suppressed slime formation by Psuudomonas aeruginosa but had little effect on the growth of the organism. The inhibitory factor was not found in broth cultures but could be extracted from cultures grown on solid agar. The inhibitory factor moderately inhibited g...

journal_title：Journal of clinical microbiology

authors： Shibl AM,Al-Sowaygh IA

更新日期：1980-02-01 00:00:00

abstract：:Adhesin-encoding operons (pap, sfa/foc, and afa) have been shown to be prevalent in Escherichia coli strains associated with urinary tract infections. A quick and sensitive assay to identify these operons was developed by using the polymerase chain reaction (PCR). Three pairs of 25-mer primers were defined from the se...

journal_title：Journal of clinical microbiology

authors： Le Bouguenec C,Archambaud M,Labigne A

更新日期：1992-05-01 00:00:00

abstract：:In titrating serum immunoglobulin G antibody to viruses by enzyme-linked immunosorbent assay, we used two rows of wells for serial twofold dilutions of the serum; in one row, a low concentration of a protein denaturant, 0.5 or 1.0 M guanidine hydrochloride, was added to the diluent so that the binding of low-avidity a...

journal_title：Journal of clinical microbiology

authors： Inouye S,Hasegawa A,Matsuno S,Katow S

更新日期：1984-09-01 00:00:00

abstract：:RotaTeq (RV5) is a widely used live attenuated pentavalent rotavirus (RV) vaccine. Although fecal shedding of RV vaccine strains persists for long time periods, it is unclear how each vaccine strain replicates in intestinal tissue and is excreted in stool. To examine this issue, we established RV5 genotype-specific re...

journal_title：Journal of clinical microbiology

authors： Higashimoto Y,Ihira M,Miyazaki Y,Kuboshiki A,Yoshinaga S,Hiramatsu H,Suzuki R,Miyata M,Miura H,Komoto S,Yukitake J,Taniguchi K,Kawamura Y,Yoshikawa T

更新日期：2018-05-25 00:00:00

abstract：:Trichosporon species are relevant etiologic agents of hospital-acquired infections. High mortality rates are attributed to Trichosporon deep-seated infections in immunocompromised individuals, making fast and accurate species identification relevant for hastening the discovery of best-targeted therapy. Recently, Trich...

journal_title：Journal of clinical microbiology

authors： de Almeida JN Jr,Favero Gimenes VM,Francisco EC,Machado Siqueira LP,Gonçalves de Almeida RK,Guitard J,Hennequin C,Colombo AL,Benard G,Rossi F

更新日期：2017-08-01 00:00:00

abstract：:We adapted a nested Aspergillus PCR to a nested qualitative real-time format on a Light Cycler system. An evaluation using 134 clinical specimens showed that the real-time PCR assay significantly reduced the time for results to be made available without compromising sensitivity and was less labor-intensive. Its use wi...

journal_title：Journal of clinical microbiology

authors： Halliday C,Wu QX,James G,Sorrell T

更新日期：2005-10-01 00:00:00

abstract：:We have isolated one sorbitol-nonfermenting (SNF) Escherichia coli O157:H7 isolate and five sorbitol-fermenting (SF) E. coli O157:H(-) isolates that do not contain Shiga toxin (Stx) genes (stx). Isolates originated from patients with diarrhea (n = 4) and hemolytic-uremic syndrome (HUS) (n = 2). All isolates harbored a...

journal_title：Journal of clinical microbiology

authors： Schmidt H,Scheef J,Huppertz HI,Frosch M,Karch H

更新日期：1999-11-01 00:00:00