Differentiation of Rhizomucor species by carbon source utilization and isoenzyme analysis.


:Nineteen Rhizomucor miehei and Rhizomucor pusillus isolates were assayed for their ability to utilize 87 various substrates as a single carbon source. Besides a difference in sucrose utilization, distinctive differences were found in the utilization of glycine, phenylalanine, and beta-alanine. Five isoenzyme systems also proved useful for the determination of markers of distinctive value at a species level. Data were used to obtain information about the genetic polymorphism of these species: a high degree of variability was found among the R. pusillus isolates, whereas the group of R. miehei isolates was more homogeneous genetically.


J Clin Microbiol


Vastag M,Papp T,Kasza Z,Vágvölgyi C




Has Abstract


1998-07-01 00:00:00












  • Comparative evaluation of the new gen-probe Mycobacterium tuberculosis amplified direct test and the semiautomated abbott LCx Mycobacterium tuberculosis assay for direct detection of Mycobacterium tuberculosis complex in respiratory and extrapulmonary spe

    abstract::Two commercial assays that detect Mycobacterium tuberculosis complex (MTB) in clinical specimens by rRNA target amplification (AMTDII) and ligase chain reaction (LCx) were evaluated. The tests were applied to 457 respiratory (n = 273) and extrapulmonary (n = 184) specimens collected from 357 patients. The results were...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Piersimoni C,Callegaro A,Scarparo C,Penati V,Nista D,Bornigia S,Lacchini C,Scagnelli M,Santini G,De Sio G

    更新日期:1998-12-01 00:00:00

  • Performance of commercial reverse line blot assays for human papillomavirus genotyping.

    abstract::The performance of three line blot assays (LBAs), the Linear Array HPV genotyping assay (LA) (Roche Diagnostics), INNO-LiPA HPV Genotyping Extra (LiPA) (Innogenetics), and the reverse hybridization assay (RH) (Qiagen), was evaluated using quantitated whole genomic human papillomavirus (HPV) plasmids (types 6, 11, 16, ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Steinau M,Onyekwuluje JM,Scarbrough MZ,Unger ER,Dillner J,Zhou T

    更新日期:2012-05-01 00:00:00

  • Septic arthritis of a native knee joint due to Corynebacterium striatum.

    abstract::We report a case of septic arthritis of a native knee joint due to Corynebacterium striatum, a rare and unusual cause of septic arthritis of native joints. The isolate was identified by a combination of phenotypic, mass spectrometric, and nucleic acid-based assays and exhibited high-level resistance to most antimicrob...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Westblade LF,Shams F,Duong S,Tariq O,Bulbin A,Klirsfeld D,Zhen W,Sakaria S,Ford BA,Burnham CA,Ginocchio CC

    更新日期:2014-05-01 00:00:00

  • PCR for detection and identification of Abiotrophia spp.

    abstract::Members of the genus Abiotrophia, formerly known as nutritionally variant streptococci, are important pathogens causing septicemia and endocarditis. Cultivation and biochemical differentiation of Abiotrophia spp. are often difficult. Based on 16S rRNA sequences, two PCR assays for detection and identification of Abiot...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Roggenkamp A,Leitritz L,Baus K,Falsen E,Heesemann J

    更新日期:1998-10-01 00:00:00

  • Isolation of species-specific protein antigens of Rickettsia typhi and Rickettsia prowazekii for immunodiagnosis and immunoprophylaxis.

    abstract::A simple procedure for the selective isolation of the protective species-specific protein antigens (SPAs) of Rickettsia typhi and Rickettsia prowazekii was developed to permit use of the SPAs in the immunodiagnosis and immunoprophylaxis of typhus infections. Although the SPAs were readily extracted from lysozyme- or d...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Dasch GA

    更新日期:1981-09-01 00:00:00

  • Family outbreak of yersiniosis.

    abstract::Yersinia enterocolitica biotype 1, serotype O:21 was isolated from feces or rectal washings of three members of one family in northwestern Saskatchewan. The three isolates gave positive pathogenicity tests in guinea pigs with cultures grown at 22 degrees C as inoculum. All three cases showed clinical symptoms consiste...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Martin T,Kasian GF,Stead S

    更新日期:1982-10-01 00:00:00

  • Quantitative detection of Chlamydia psittaci and C. pecorum by high-sensitivity real-time PCR reveals high prevalence of vaginal infection in cattle.

    abstract::Bovine vaginal cytobrush specimens were analyzed for the presence of Chlamydia spp. by a high-sensitivity, high-specificity quantitative PCR. The 53% prevalence of low-level Chlamydia psittaci and C. pecorum genital infection detected in virgin heifers suggests predominantely extragenital transmission of Chlamydia in ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: DeGraves FJ,Gao D,Hehnen HR,Schlapp T,Kaltenboeck B

    更新日期:2003-04-01 00:00:00

  • Optimal method of collection of first-void urine for diagnosis of Chlamydia trachomatis infection in men.

    abstract::First-void urine (FVU) is the preferred specimen for the diagnosis of urogenital Chlamydia trachomatis infection in men. We have developed FirstBurst, a urine collection device that collects the first 4 to 5 ml of FVU and yields a specimen with a sixfold higher C. trachomatis organism load than the regular urine cup b...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Wisniewski CA,White JA,Michel CE,Mahilum-Tapay L,Magbanua JP,Nadala EC Jr,Barber PJ,Goh BT,Lee HH

    更新日期:2008-04-01 00:00:00

  • Identification of urovirulence traits in Escherichia coli by comparison of urinary and rectal E. coli isolates from dogs with urinary tract infection.

    abstract::Spontaneously occurring urinary tract infection (UTI) in dogs was exploited as an experiment of nature to gain insights into UTI pathogenesis in humans. Concurrent urinary and rectal Escherichia coli isolates from 37 dogs with UTI were compared with respect to phylogenetic background, O antigens, and extended virulenc...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Johnson JR,Kaster N,Kuskowski MA,Ling GV

    更新日期:2003-01-01 00:00:00

  • Diagnosis of invasive amebiasis by enzyme-linked immunosorbent assay of saliva to detect amebic lectin antigen and anti-lectin immunoglobulin G antibodies.

    abstract::Saliva from subjects with amebic liver abscess (ALA), acute amebic colitis, asymptomatic infection with Entamoeba histolytica or Entamoeba dispar, and uninfected controls was tested by enzyme-linked immunosorbent assay (ELISA) for the presence of E. histolytica galactose-inhibitable lectin antigen and salivary immunog...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Abd-Alla MD,Jackson TF,Reddy S,Ravdin JI

    更新日期:2000-06-01 00:00:00

  • Molecular evidence for multiple host-specific strains in the genus Rhinosporidium.

    abstract::The taxonomic relationship of Rhinosporidium seeberi with other organisms remained controversial for over a century. Recently, molecular studies have shown R. seeberi to be a protistal microbe in the newly described class Mesomycetozoea at the animal-fungal boundary. Phylogenetic analyses of R. seeberi using 18S small...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Silva V,Pereira CN,Ajello L,Mendoza L

    更新日期:2005-04-01 00:00:00

  • Serotype-specific identification of polioviruses by PCR using primers containing mixed-base or deoxyinosine residues at positions of codon degeneracy.

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    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Kilpatrick DR,Nottay B,Yang CF,Yang SJ,Da Silva E,Peñaranda S,Pallansch M,Kew O

    更新日期:1998-02-01 00:00:00

  • Viral studies on human brain culture retrieved from cold storage.

    abstract::Tissue cultures established from brain tissues of six patients with various chronic, degenerative diseases did not show evidence of viral agents. Sucrose gradient analysis of radioactively labeled culture fluid from two patients failed to reveal the presence of any incomplete or defective virus. Extracellular fluids f...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Lennette ET

    更新日期:1975-05-01 00:00:00

  • A Complete Genome Screening Program of Clinical Methicillin-Resistant Staphylococcus aureus Isolates Identifies the Origin and Progression of a Neonatal Intensive Care Unit Outbreak.

    abstract::Whole-genome sequencing (WGS) of Staphylococcus aureus is increasingly used as part of infection prevention practices. In this study, we established a long-read technology-based WGS screening program of all first-episode methicillin-resistant Staphylococcus aureus (MRSA) blood infections at a major urban hospital. A s...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Sullivan MJ,Altman DR,Chacko KI,Ciferri B,Webster E,Pak TR,Deikus G,Lewis-Sandari M,Khan Z,Beckford C,Rendo A,Samaroo F,Sebra R,Karam-Howlin R,Dingle T,Hamula C,Bashir A,Schadt E,Patel G,Wallach F,Kasarskis A,Gi

    更新日期:2019-11-22 00:00:00

  • Use of colorimetric culture methods for detection of Mycobacterium tuberculosis complex isolates from sputum samples in resource-limited settings.

    abstract::Despite recent advances, tuberculosis (TB) diagnosis remains imperfect in resource-limited settings due to its complexity and costs, poor sensitivity of available tests, or long times to reporting. We present a report on the use of colorimetric methods, based on the detection of mycobacterial growth using colorimetric...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Boum Y 2nd,Orikiriza P,Rojas-Ponce G,Riera-Montes M,Atwine D,Nansumba M,Bazira J,Tuyakira E,De Beaudrap P,Bonnet M,Page AL

    更新日期:2013-07-01 00:00:00

  • Role of Helicobacter species in Chinese patients with inflammatory bowel disease.

    abstract::Based on 16S rRNA gene PCR, no significant difference was observed in rates of detection of Helicobacter species in intestinal biopsy specimens from 160 Chinese inflammatory bowel disease (IBD) patients (10%) and 80 controls (6.3%). By using a [(13)C]urea breath test, the H. pylori infection rate in 208 Chinese IBD pa...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Zhang S,Zhong B,Chao K,Xiao Y,Cui Y,Gao X,Chen B,He Y,Hu P,Chen M,Mitchell HM

    更新日期:2011-05-01 00:00:00

  • Performance of a polymer-based DNA chip platform in detection and genotyping of human papillomavirus in clinical samples.

    abstract::Human papillomavirus (HPV) plays a key role in the development of cervical and laryngeal cancers. The aim of our study was to compare the performance of a new hydrogel-based HPV genotyping biochip assay (Biochip) to a commercially available and CE-marked conventional PCR followed by reverse hybridization (GenID-PCR). ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Schenk T,Brandstetter T,Zur Hausen A,Alt-Mörbe J,Huzly D,Rühe J

    更新日期:2009-05-01 00:00:00

  • Earlier positivity of central-venous- versus peripheral-blood cultures is highly predictive of catheter-related sepsis.

    abstract::To diagnose catheter-related sepsis without removing the catheter, we evaluated the differential positivity times of cultures of blood drawn simultaneously from central venous catheter and peripheral sites. In a 450-bed cancer reference center, simultaneous central- and peripheral-blood cultures were prospectively per...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Blot F,Schmidt E,Nitenberg G,Tancrède C,Leclercq B,Laplanche A,Andremont A

    更新日期:1998-01-01 00:00:00

  • Frequency of microbiologically correct antibiotic therapy increased by infectious disease consultations and microbiological results.

    abstract::In a prospective observational study of bacteremic patients we ascertained the influence of different parts of culture results on the correctness of empirical antibiotic therapy. Ninety-three bacteremic patients requiring antibiotic treatment were included. Patients who had consultations with an infectious disease con...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Kerremans JJ,Verbrugh HA,Vos MC

    更新日期:2012-06-01 00:00:00

  • Detection of group B streptococcal antigen in body fluids by a latex-coupled monoclonal antibody assay.

    abstract::A commercially available latex agglutination reagent, Directigen (Hynson, Westcott & Dunning, Div. Becton Dickinson & Co., Baltimore, Md.), in which a murine monoclonal antibody to group B streptococcal (GBS) antigen is the active component, was evaluated by using body fluid specimens from 94 sick infants. Antigen was...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Rench MA,Metzger TG,Baker CJ

    更新日期:1984-11-01 00:00:00

  • Comparison of PCR-ribotyping, arbitrarily primed PCR, and pulsed-field gel electrophoresis for typing Clostridium difficile.

    abstract::Clostridium difficile is now recognized as the major agent responsible for nosocomial diarrhea in adults. Among the genotyping methods available, arbitrarily primed PCR (AP-PCR), PCR-ribotyping, and pulsed-field gel electrophoresis (PFGE) have been widely used for investigating outbreaks of C. difficile infections. Ho...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Bidet P,Lalande V,Salauze B,Burghoffer B,Avesani V,Delmée M,Rossier A,Barbut F,Petit JC

    更新日期:2000-07-01 00:00:00

  • Screening and diagnostic performance of enzyme immunoassay for antibody to lymphadenopathy-associated virus.

    abstract::In a multicenter cooperative study, an enzyme immunoassay (EIA) using purified antigen of lymphadenopathy-associated virus was compared with radioimmune precipitation (RIP) for detection of antibody to human immunodeficiency virus (HIV) in 634 patients with acquired immunodeficiency syndrome or related conditions, 687...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Handsfield HH,Wandell M,Goldstein L,Shriver K

    更新日期:1987-05-01 00:00:00

  • Single real-time reverse transcription-PCR assay for detection and quantification of genetically diverse HIV-1, SIVcpz, and SIVgor strains.

    abstract::Although antiretroviral treatment availability has improved, the virological monitoring of patients remains largely uneven across regions. In addition, viral quantification tests are suffering from human immunodeficiency virus type 1 (HIV-1) genetic diversity, fueled by the emergence of new recombinants and of lentivi...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Etienne L,Eymard-Duvernay S,Aghokeng A,Butel C,Monleau M,Peeters M

    更新日期:2013-03-01 00:00:00

  • Infection of the olecranon bursa by Anthopsis deltoidea.

    abstract::Anthopsis deltoidea was found to be the cause of an olecranon bursitis in a 79-year-old golfer. Serial histological sections of the olecranon bursa showed faintly stained, brown-walled, septate, hyphal elements in the centers of the necrotic debris. The combination of bursectomy and flucytosine treatment cured the inf...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Kwon-Chung KJ,Droller DD

    更新日期:1984-08-01 00:00:00

  • Mycobacterial cross contamination during radiometric culturing.

    abstract::Pseudobacteremias in blood cultures performed on the BACTEC radiometric blood culture system have been reported. We report three cases of cross contamination with Mycobacterium avium that occurred when mycobacteria were cultured with the BACTEC 460 TB system. Malfunction of the needle sterilization heating block was d...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Vannier AM,Tarrand JJ,Murray PR

    更新日期:1988-09-01 00:00:00

  • Novel method for genotyping hepatitis B virus on the basis of TaqMan real-time PCR.

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    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Malmström S,Berglin-Enquist I,Lindh M

    更新日期:2010-04-01 00:00:00

  • Cultivation and characterization of human rotaviruses with "super short" RNA patterns.

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    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Albert MJ,Unicomb LE,Bishop RF

    更新日期:1987-01-01 00:00:00

  • Detecting bunyaviruses of the Bunyamwera and California serogroups by a PCR technique.

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    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Kuno G,Mitchell CJ,Chang GJ,Smith GC

    更新日期:1996-05-01 00:00:00

  • Discrimination of antibody to herpes B virus from antibody to herpes simplex virus types 1 and 2 in human and macaque sera.

    abstract::The antigenic cross-reactive characteristics of herpes B virus and herpes simplex virus (HSV) type 1 (HSV-1) and HSV-2 are responsible for false-positive diagnoses by serological assays in humans and macaques. In the present study, we developed a fluorometric indirect enzyme-linked immunosorbent assay (ELISA) with rec...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Fujima A,Ochiai Y,Saito A,Omori Y,Noda A,Kazuyama Y,Shoji H,Tanabayashi K,Ueda F,Yoshikawa Y,Hondo R

    更新日期:2008-01-01 00:00:00

  • Assessment of Etest as an alternative to agar dilution for antimicrobial susceptibility testing of Neisseria gonorrhoeae.

    abstract::We studied whether the Etest can be used as an alternative to agar dilution to determine antimicrobial susceptibilities of ceftriaxone, cefixime, and cefpodoxime in Neisseria gonorrhoeae surveillance. One hundred fifteen clinical and laboratory isolates of N. gonorrhoeae were tested following the Clinical Laboratory I...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Liu H,Taylor TH Jr,Pettus K,Trees D

    更新日期:2014-05-01 00:00:00