Impact of changes in Clostridium difficile testing practices on stool rejection policies and C. difficile positivity rates across multiple laboratories in the United States.

abstract：:We report on the development of a fully automated real-time PCR assay for the quantitative detection of hepatitis B virus (HBV) DNA in plasma with EDTA (EDTA plasma). The MagNA Pure LC instrument was used for automated DNA purification and automated preparation of PCR mixtures. Real-time PCR was performed on the Light...

journal_title：Journal of clinical microbiology

authors： Leb V,Stöcher M,Valentine-Thon E,Hölzl G,Kessler H,Stekel H,Berg J

更新日期：2004-02-01 00:00:00

abstract：:Drug-resistant tuberculosis (TB) is a major threat to TB control worldwide. Globally, only 40% of the 340,000 notified TB patients estimated to have multidrug-resistant-TB (MDR-TB) were detected in 2015. This study was carried out to evaluate the utility of high-resolution melt curve analysis (HRM) for the rapid and d...

journal_title：Journal of clinical microbiology

authors： Anthwal D,Gupta RK,Bhalla M,Bhatnagar S,Tyagi JS,Haldar S

更新日期：2017-06-01 00:00:00

abstract：:In a multicenter study, the consistency of international units expressed by five commercially available rubella virus immunoglobulin G kits was evaluated. The linearity and within-run and between-run precision were determined for each kit. All kits demonstrated good linearity and had within-run and between-run precisi...

journal_title：Journal of clinical microbiology

authors： Dimech W,Bettoli A,Eckert D,Francis B,Hamblin J,Kerr T,Ryan C,Skurrie I

更新日期：1992-03-01 00:00:00

abstract：:We compared the results of Gram staining and culture of cerebrospinal fluid to results obtained with a rapid PCR assay for the diagnosis of meningococcal meningitis in 281 cases of suspected bacterial meningitis. PCR had a sensitivity of 97% compared to a sensitivity of 55% for culture, and the PCR specificity was 99....

journal_title：Journal of clinical microbiology

authors： Richardson DC,Louie L,Louie M,Simor AE

更新日期：2003-08-01 00:00:00

abstract：:In titrating serum immunoglobulin G antibody to viruses by enzyme-linked immunosorbent assay, we used two rows of wells for serial twofold dilutions of the serum; in one row, a low concentration of a protein denaturant, 0.5 or 1.0 M guanidine hydrochloride, was added to the diluent so that the binding of low-avidity a...

journal_title：Journal of clinical microbiology

authors： Inouye S,Hasegawa A,Matsuno S,Katow S

更新日期：1984-09-01 00:00:00

abstract：:A premarket trial of the Becton Dickinson Directigen respiratory syncytial virus membrane-based enzyme immunoassay compared the test with virus isolation for the detection of respiratory syncytial virus in 583 nasopharyngeal aspirates. After modification, the Directigen test showed a sensitivity of 83% and a specifici...

journal_title：Journal of clinical microbiology

authors： Kok T,Barancek K,Burrell CJ

更新日期：1990-06-01 00:00:00

abstract：:Thirty-three strains of Cryptococcus neoformans were isolated from clinical specimens, including specimens from AIDS patients in Brazil, and were classified into two serotypes; we detected 31 and 2 strains of serotypes A and B, respectively. Random amplified polymorphic DNA (RAPD) fingerprint pattern analyses of these...

journal_title：Journal of clinical microbiology

authors： Aoki FH,Imai T,Tanaka R,Mikami Y,Taguchi H,Nishimura NF,Nishimura K,Miyaji M,Schreiber AZ,Branchini ML

更新日期：1999-02-01 00:00:00

abstract：:A toxigenic Corynebacterium diphtheriae biovar mitis sequence type 136 (ST136) strain was recovered from a toe infection of an unvaccinated patient recently returned from India. The isolate was resistant to clindamycin, erythromycin (ermX positive), tetracycline, and trimethoprim-sulfamethoxazole, intermediate to ceft...

journal_title：Journal of clinical microbiology

authors： Mina NV,Burdz T,Wiebe D,Rai JS,Rahim T,Shing F,Hoang L,Bernard K

更新日期：2011-11-01 00:00:00

abstract：:MICs and minimal bactericidal concentrations (MBCs) were evaluated for the four antibiotics azithromycin, amoxicillin, ceftriaxone, and doxycycline against the three main genospecies of Borrelia burgdorferi sensu lato. In MBC testing, statistically significant differences between the genospecies could be found in 7 ou...

journal_title：Journal of clinical microbiology

authors： Sicklinger M,Wienecke R,Neubert U

更新日期：2003-04-01 00:00:00

abstract：:This report describes the first well-documented reported case of the recovery of Blastomyces dermatitidis from the blood of a patient with disseminated blastomycosis. The isolate was recovered by using a lysis-centrifugation method and inhibitory mold agar after 6 days of incubation at 30 degrees C. One of six blood s...

journal_title：Journal of clinical microbiology

authors： Musial CE,Wilson WR,Sinkeldam IR,Roberts GD

更新日期：1987-08-01 00:00:00

abstract：:From January 2011 to December 2013, a total of 262 samples, from 188 patients suspected of having syphilis were tested for the presence of treponemal DNA by PCR amplification of five chromosomal loci, including the polA (TP0105), tmpC (TP0319), TP0136, TP0548, and 23S rRNA genes. Altogether, 146 samples from 103 patie...

journal_title：Journal of clinical microbiology

authors： Grillová L,Pĕtrošová H,Mikalová L,Strnadel R,Dastychová E,Kuklová I,Kojanová M,Kreidlová M,Vaňousová D,Hercogová J,Procházka P,Zákoucká H,Krchňáková A,Vašků V,Šmajs D

更新日期：2014-10-01 00:00:00

abstract：:An accurate, rapid, and inexpensive method was developed for detecting and enumerating bacteria adherent to Foley urinary catheters based on malachite green staining of acridine orange-prestained specimens. This method has proven to be quick and reliable and will find application in quantitative studies of biomaterial...

journal_title：Journal of clinical microbiology

authors： Ladd TI,Schmiel D,Nickel JC,Costerton JW

更新日期：1985-06-01 00:00:00

abstract：:Four serotypes of Cryptococcus neoformans designated A, B, C, and D are currently recognized. Although an agglutination test is most often used to serotype C. neoformans in cultures, this test is not appropriate for typing the fungus in fixed tissues. A study to prepare fluorescent-antibody reagents for typing C. neof...

journal_title：Journal of clinical microbiology

authors： Kaplan W,Bragg SL,Crane S,Ahearn DG

更新日期：1981-09-01 00:00:00

abstract：:Three strains of a Pasteurella haemolytica-like bacterium were isolated from lesions of a progressive granuloma of cattle in southern Brazil. Their characteristics and their differentiation from P. haemolytica varieties and Actinobacillus lignieresii are described. The name "Pasteurella granulomatis" is proposed for t...

journal_title：Journal of clinical microbiology

authors： Ribeiro GA,Carter GR,Frederiksen W,Riet-Correa F

更新日期：1989-06-01 00:00:00

abstract：:Rhesus and cynomolgus monkey kidney tissue cultures and two continuous lines, Madin-Darby canine kidney (MDCK) and LLC-MK2, were compared in titrations and isolations of influenza and parainfluenza viruses. Tube cultures were inoculated with laboratory virus strains or stored patient specimens and observed for hemadso...

journal_title：Journal of clinical microbiology

authors： Frank AL,Couch RB,Griffis CA,Baxter BD

更新日期：1979-07-01 00:00:00

abstract：:Sensitivity of the enzyme-linked immunosorbent assay for detecting serum antibodies to rotavirus was greatly enhanced when rotavirus particles were fragmented by chaotropic agents (NaSCN or guanidine hydrochloride) before adsorption of the antigens to the solid phase. For detecting fecal antibodies, the addition of fe...

journal_title：Journal of clinical microbiology

authors： Inouye S,Matsuno S,Yamaguchi H

更新日期：1984-02-01 00:00:00

abstract：:The factors that regulate cytomegalovirus (CMV) excretion from the genitourinary tract are poorly understood. To assess the role of cell-mediated immunity in such excretion, a CMV-specific mononuclear blastogenesis assay was used to study a predominantly lower-socioeconomic-status population of 92 healthy nonpregnant ...

journal_title：Journal of clinical microbiology

authors： Faix RG,Zweig SE,Kummer JF,Moore D,Lang DJ

更新日期：1983-04-01 00:00:00

abstract：:A new PCR-reverse dot blot hybridization (RDBH) assay was developed for the rapid identification of Mycobacterium species in clinical isolates. The assay, which targets the 16S rRNA, was evaluated for 27 mycobacterial reference strains and 340 clinical isolates that were simultaneously identified by DNA sequencing and...

journal_title：Journal of clinical microbiology

authors： Wu X,Zhang J,Liang J,Lu Y,Li H,Li C,Yue J,Zhang L,Liu Z

更新日期：2007-06-01 00:00:00

abstract：:The babA2 and cagA genes were investigated in 208 Brazilian Helicobacter pylori strains. A strong association between babA2 and duodenal ulcer or gastric carcinoma was observed, even after adjusting for confounding factors, such as age, gender, and cagA status. cagA-positive strains were also independently associated ...

journal_title：Journal of clinical microbiology

authors： Oliveira AG,Santos A,Guerra JB,Rocha GA,Rocha AM,Oliveira CA,Cabral MM,Nogueira AM,Queiroz DM

更新日期：2003-08-01 00:00:00

abstract：:The ability of Staphylococcus aureus to clot plasma through conformational activation of prothrombin by staphylocoagulase is used to distinguish S. aureus from coagulase-negative staphylococci. We show that while the direct thrombin inhibitor dabigatran inhibits staphylocoagulase activity, the clinical use of dabigatr...

journal_title：Journal of clinical microbiology

authors： Vanassche T,Verhaegen J,Peetermans WE,Hoylaerts MF,Verhamme P

更新日期：2010-11-01 00:00:00

abstract：:A collection of 54 unrelated Streptococcus agalactiae strains isolated from cerebrospinal fluid samples from neonates and 60 unrelated strains isolated from carriers that had been previously studied by multilocus enzyme electrophoresis (R. Quentin, H. Huet, F.-S. Wang, P. Geslin, A. Goudeau, and R. K. Selander, J. Cli...

journal_title：Journal of clinical microbiology

authors： Chatellier S,Ramanantsoa C,Harriau P,Rolland K,Rosenau A,Quentin R

更新日期：1997-10-01 00:00:00

abstract：:A study to compare the polymerase chain reaction (PCR) test with the cell culture method in diagnosing urogenital Chlamydia trachomatis infections was performed. From 497 patients (212 women, 285 men) attending an outpatient clinic for sexually transmitted diseases, a total of 814 samples (female patients, cervix and ...

journal_title：Journal of clinical microbiology

authors： Vogels WH,van Voorst Vader PC,Schröder FP

更新日期：1993-05-01 00:00:00

abstract：:Developing interpretive breakpoints for any given organism-drug combination requires integration of the MIC distribution, pharmacokinetic and pharmacodynamic parameters, and the relationship between the in vitro activity and outcome from both in vivo and clinical studies. Using data generated by standardized broth mic...

journal_title：Journal of clinical microbiology

authors： Pfaller MA,Diekema DJ,Rex JH,Espinel-Ingroff A,Johnson EM,Andes D,Chaturvedi V,Ghannoum MA,Odds FC,Rinaldi MG,Sheehan DJ,Troke P,Walsh TJ,Warnock DW

更新日期：2006-03-01 00:00:00

abstract：:The fourth-generation QuantiFERON test for tuberculosis infection, QuantiFERON-TB Gold Plus (QFT-Plus) has replaced the earlier version, QuantiFERON-TB Gold In-Tube (QFT-GIT). A clinical need exists for information about agreement between QFT-Plus and other tests. We conducted this study to assess agreement of test re...

journal_title：Journal of clinical microbiology

authors： Venkatappa TK,Punnoose R,Katz DJ,Higgins MP,Banaei N,Graviss EA,Belknap RW,Ho CS

更新日期：2019-10-23 00:00:00

abstract：:Recent reports of natural paratuberculosis (or Johne's disease) in rabbits, foxes, and stoats has focused debate on the presence and importance of wildlife reservoirs in the epidemiology of this disease. This paper describes an extensive study investigating 18 nonruminant wildlife species for evidence of paratuberculo...

journal_title：Journal of clinical microbiology

authors： Beard PM,Daniels MJ,Henderson D,Pirie A,Rudge K,Buxton D,Rhind S,Greig A,Hutchings MR,McKendrick I,Stevenson K,Sharp JM

更新日期：2001-04-01 00:00:00

abstract：:Decreasing the time to species identification and antibiotic susceptibility determination of strains recovered from patients with bacteremia significantly decreases morbidity and mortality. Herein, we validated a method to identify Gram-negative bacteria directly from positive blood culture medium using the Bruker MAL...

journal_title：Journal of clinical microbiology

authors： Wimmer JL,Long SW,Cernoch P,Land GA,Davis JR,Musser JM,Olsen RJ

更新日期：2012-07-01 00:00:00

abstract：:We demonstrate that all nine species of the Burkholderia cepacia complex can express the mucoid phenotype. A survey of clinical isolates showed that strains of B. cenocepacia, the most virulent species of the complex, are most frequently nonmucoid. Additionally, isolates from patients with chronic infections can conve...

journal_title：Journal of clinical microbiology

authors： Zlosnik JE,Hird TJ,Fraenkel MC,Moreira LM,Henry DA,Speert DP

更新日期：2008-04-01 00:00:00

abstract：:While foreign-born persons constitute only 11% of the population in the state of Rhode Island, they account for more than 65% of incident tuberculosis (TB) annually. We investigated the molecular-epidemiological differences between foreign-born and U.S.-born TB patients to estimate the degree of recent transmission an...

journal_title：Journal of clinical microbiology

authors： Vanhomwegen J,Kwara A,Martin M,Gillani FS,Fontanet A,Mutungi P,Crellin J,Obaro S,Gosciminski M,Carter EJ,Rastogi N

更新日期：2011-03-01 00:00:00

abstract：:We report the recovery of Histoplasma capsulatum from blood specimens cultured for Mycobacterium sp. in BACTEC 13A radiometric medium. H. capsulatum was recovered from six of eight blood specimens submitted for mycobacterial cultures from five human immunodeficiency virus-positive individuals. Initial positive metabol...

journal_title：Journal of clinical microbiology

authors： Merz WG,Kodsy S,Merz CS

更新日期：1992-01-01 00:00:00

abstract：:We compared whole blood dried on filter paper to the standard assay with frozen cell-free plasma for use in the quantitation of the human immunodeficiency virus RNA load in blood. RNA values from filter paper, corrected for the hematocrit, gave results comparable to those of the standard assay in terms of sensitivity ...

journal_title：Journal of clinical microbiology

authors： Fiscus SA,Brambilla D,Grosso L,Schock J,Cronin M

更新日期：1998-01-01 00:00:00