Application of a simple multiplex PCR to aid in routine work of the mycobacterium reference laboratory.

abstract：:Lipopolysaccharide components 3-deoxy-D-manno-2-octulosonic acid and L-glycero-D-manno-heptose were detected in hydrolysates from whole cells of Neisseria elongata by gas-liquid chromatography. Cells from a single plate were hydrolyzed, and carbohydrate components were converted to aldononitrile and O-methyloxime acet...

journal_title：Journal of clinical microbiology

authors： Guerrant GO,Moss CW

更新日期：1986-03-01 00:00:00

abstract：:Anthopsis deltoidea was found to be the cause of an olecranon bursitis in a 79-year-old golfer. Serial histological sections of the olecranon bursa showed faintly stained, brown-walled, septate, hyphal elements in the centers of the necrotic debris. The combination of bursectomy and flucytosine treatment cured the inf...

journal_title：Journal of clinical microbiology

authors： Kwon-Chung KJ,Droller DD

更新日期：1984-08-01 00:00:00

abstract：:The aim of this study was to develop a PCR and reverse line blot hybridization (PCR-RLB) macroarray assay based on 16S-23S rRNA gene internal transcribed spacer sequences for the identification and differentiation of 34 mycobacterial species or subspecies. The performance of the PCR-RLB assay was assessed and validate...

journal_title：Journal of clinical microbiology

authors： Xiong L,Kong F,Yang Y,Cheng J,Gilbert GL

更新日期：2006-10-01 00:00:00

abstract：:We examined clinical (pulmonary cryptococcosis and cryptococcal meningitis) and environmental (pigeon excreta) isolates of Cryptococcus neoformans var. neoformans (serotype A) in the southern Japanese prefecture of Nagasaki. The random amplified polymorphic DNA profiles obtained by using three primers revealed six pat...

journal_title：Journal of clinical microbiology

authors： Yamamoto Y,Kohno S,Koga H,Kakeya H,Tomono K,Kaku M,Yamazaki T,Arisawa M,Hara K

更新日期：1995-12-01 00:00:00

abstract：:Sixty-one Salmonella enterica serovar Typhimurium isolates of animal and human origin, matched by phage type, antimicrobial resistance pattern, and place of isolation, were analyzed by microbiological and molecular techniques, including pulsed-field gel electrophoresis (PFGE) and plasmid profiling. PFGE identified 10 ...

journal_title：Journal of clinical microbiology

authors： Cooke FJ,Wain J,Fookes M,Ivens A,Thomson N,Brown DJ,Threlfall EJ,Gunn G,Foster G,Dougan G

更新日期：2007-08-01 00:00:00

abstract：:In this work, we describe the assembly of a synthetic gene coding for several antigenic determinants found in different Leishmania infantum antigens. Selected epitopes were derived from the ribosomal proteins LiP2a, LiP2b, and LiP0 and from the histone H2A. The resulting gene was overexpressed in Escherichia coli eith...

journal_title：Journal of clinical microbiology

authors： Soto M,Requena JM,Quijada L,Alonso C

更新日期：1998-01-01 00:00:00

abstract：:An in situ DNA hybridization kit for cytomegalovirus (CMV) was evaluated for the detection of CMV in centrifugation culture. Of 61 clinical specimens, 17 (27.8%) were positive for CMV by monoclonal antibody staining following centrifugation. Of the 17 positive specimens, 15 were detected by DNA hybridization (24.5%). ...

journal_title：Journal of clinical microbiology

authors： Gleaves CA,Hursh DA,Rice DH,Meyers JD

更新日期：1989-01-01 00:00:00

abstract：:Streptococcus pneumoniae plays an important role in causing acute exacerbations in patients with chronic respiratory disease. However, few data are available regarding pneumococcal persistence in adult patients with chronic respiratory diseases. Fifty pneumococci recovered from sputum samples (1995 to 2010) from 13 ad...

journal_title：Journal of clinical microbiology

authors： Domenech A,Ardanuy C,Balsalobre L,Marti S,Calatayud L,De la Campa AG,Brueggemann AB,Liñares J

更新日期：2012-12-01 00:00:00

abstract：:We studied the use of dried spots of bodily fluids (plasma, whole blood, and mother's milk) on filter paper as a means of sample collection and storage for human immunodeficiency virus type 1 (HIV-1) viral load testing under stringent field conditions. Plasma placed directly in lysis buffer, which is customarily used ...

journal_title：Journal of clinical microbiology

authors： Ayele W,Schuurman R,Messele T,Dorigo-Zetsma W,Mengistu Y,Goudsmit J,Paxton WA,de Baar MP,Pollakis G

更新日期：2007-03-01 00:00:00

abstract：:We studied the plasmid profiles of 136 Shigella isolates in Bangladesh to determine whether plasmid profiles could be used for differentiation of strains for epidemiological studies. Many different plasmid patterns were observed within each species, indicating that many genetically different strains of Shigella are re...

journal_title：Journal of clinical microbiology

authors： Tacket CO,Shahid N,Huq MI,Alim AR,Cohen ML

更新日期：1984-08-01 00:00:00

abstract：:For prevention and control of gonorrhea, an objective, highly discriminating, and reproducible molecular epidemiological characterization of Neisseria gonorrhoeae is essential. In the present study, in pursuance of providing such qualities, pyrosequencing technology, a fast real-time DNA sequence analysis, was applied...

journal_title：Journal of clinical microbiology

authors： Unemo M,Olcén P,Jonasson J,Fredlund H

更新日期：2004-07-01 00:00:00

abstract：:Ureaplasma urealyticum is a commensal organism of the lower genital tract of females, but in a subpopulation of individuals, it can invade the upper genital tract. It is a significant cause of chorioamnionitis and neonatal morbidity and mortality. There are 14 recognized serovars of U. urealyticum; these can be divide...

journal_title：Journal of clinical microbiology

authors： Teng LJ,Zheng X,Glass JI,Watson HL,Tsai J,Cassell GH

更新日期：1994-06-01 00:00:00

abstract：:Human milk obtained from 21 American nursing mothers was studied for the presence of secretory immunoglobulin A antibody to rotavirus, the most common etiological agent of infantile gastroenteritis. Antibody was quantitated by adaptation of a recently described solid-phase radioimmunoassay technique that employs simia...

journal_title：Journal of clinical microbiology

authors： Cukor G,Blacklow NR,Capozza FE,Panjvani ZF,Bednarek F

更新日期：1979-01-01 00:00:00

abstract：:This study evaluated the feasibility of a molecular strategy based on identification of Mycobacterium tuberculosis by IS6110 PCR or Cobas Amplicor PCR, and rpoB PCR-DNA sequencing of the 81-bp rifampin resistance determining region (RRDR) for direct detection of rifampin resistance in respiratory specimens. A collecti...

journal_title：Journal of clinical microbiology

authors： Yam WC,Tam CM,Leung CC,Tong HL,Chan KH,Leung ET,Wong KC,Yew WW,Seto WH,Yuen KY,Ho PL

更新日期：2004-10-01 00:00:00

abstract：:The diagnosis of filarial infections among individuals residing in areas where the disease is not endemic requires both strong clinical suspicion and expert training in infrequently practiced parasitological methods. Recently developed filarial molecular diagnostic assays are highly sensitive and specific but have lim...

journal_title：Journal of clinical microbiology

authors： Fink DL,Fahle GA,Fischer S,Fedorko DF,Nutman TB

更新日期：2011-01-01 00:00:00

abstract：:Human metapneumovirus (hMPV) is a recently discovered paramyxovirus that is known to cause respiratory tract infections in children and immunocompromised individuals. Given the difficulties of identifying hMPV by conventional culture, molecular techniques could improve the detection of this virus in clinical specimens...

journal_title：Journal of clinical microbiology

authors： Dare R,Sanghavi S,Bullotta A,Keightley MC,George KS,Wadowsky RM,Paterson DL,McCurry KR,Reinhart TA,Husain S,Rinaldo CR

更新日期：2007-02-01 00:00:00

abstract：:An indirect immunofluorescence test with total anti-human immunoglobulin conjugate (IgG,A,M-IIF) can be used for joint detection of immunoglobulin A (IgA) and IgM antibodies, provided serum IgG is previously absorbed with anti-human IgG. To determine the validity of the IgG,A,M-IIF assay with absorbed sera, the result...

journal_title：Journal of clinical microbiology

authors： Arcavi M,Orfus G,Griemberg G

更新日期：1997-06-01 00:00:00

abstract：:The effect of supplementing nutrient substrate with various combinations of concentrations of oleic and linoleic acids on the growth of 11 strains of Staphylococcus aureus was assessed. Whereas increasing the concentration of linoleic acid by itself greatly diminished the growth of all 11 strains, concomitant increase...

journal_title：Journal of clinical microbiology

authors： Campbell IM,Crozier DN,Pawagi AB,Buivids IA

更新日期：1983-08-01 00:00:00

abstract：:We are reporting on a nosocomial outbreak of 213 cases of vancomycin-resistant enterococcus infection involving 2,812 enterococcal isolates from patients over a period of 36 months. In 1990, the Enterococcus faecium vancomycin susceptibility rate was found to be 85.7% (36 of 42 cases), and an incidence of 10.9% (42 of...

journal_title：Journal of clinical microbiology

authors： Boyle JF,Soumakis SA,Rendo A,Herrington JA,Gianarkis DG,Thurberg BE,Painter BG

更新日期：1993-05-01 00:00:00

abstract：:Commercial chemiluminescent DNA probes (Accuprobe; Gen-Probe, San Diego, Calif.) for the identification of Mycobacterium tuberculosis (MTB) complex, M. avium complex (MAC), M. gordonae, and M. kansasii were evaluated with 134 clinical isolates. These included 36 MTB complex, 40 MAC, 27 M. gordonae, 9 M. kansasii, and ...

journal_title：Journal of clinical microbiology

authors： Lebrun L,Espinasse F,Poveda JD,Vincent-Levy-Frebault V

更新日期：1992-09-01 00:00:00

abstract：:A nationwide study on candidemia was conducted in Iceland from 1980 to 1999. The annual incidence increased from 1.4 cases/100,000 inhabitants/year between 1980 and 1984 to 4.9 cases/100,000 inhabitants/year between 1995 and 1999 (P < 0.0001). Candidemia episodes at university hospitals increased from 0.15/1,000 admis...

journal_title：Journal of clinical microbiology

authors： Asmundsdóttir LR,Erlendsdóttir H,Gottfredsson M

更新日期：2002-09-01 00:00:00

abstract：:Capnocytophaga ochracea is a gram-negative, fusiform bacillus which is part of the normal human oral flora. This organism is often isolated from periodontal lesions of patients with periodontitis and is associated with sepsis in granulocytopenic patients. We report here a case of endocarditis caused by C. ochracea. ...

journal_title：Journal of clinical microbiology

authors： Buu-Hoi AY,Joundy S,Acar JF

更新日期：1988-05-01 00:00:00

abstract：:This study investigated a rare area of endemicity with a high prevalence of both hepatitis B virus (HBV) and hepatitis C virus (HCV) infections, in Putian County, China. Among 1,050 subjects, the overall prevalence of HBsAg seropositivity was 15.8%, and that of anti-HCV seropositivity was 28.9%. Intrafamilial viral tr...

journal_title：Journal of clinical microbiology

authors： Lu WP,Lin GX,Shi S,Dong JH

更新日期：2012-06-01 00:00:00

abstract：:From January to December 2007, 973 stool specimens were prospectively collected from children hospitalized for gastroenteritis signs or from neonates and premature cases who were born in two French hospital settings in the north of France. They were tested by rapid enzyme immunoassay (EIA) analyses for rotavirus and a...

journal_title：Journal of clinical microbiology

authors： Tran A,Talmud D,Lejeune B,Jovenin N,Renois F,Payan C,Leveque N,Andreoletti L

更新日期：2010-05-01 00:00:00

abstract：:Conserved primers were used to PCR amplify 95% of the Helicobacter hepaticus 16S rRNA gene. Its sequence was determined and aligned to those of related bacteria, enabling the selection of primers to highly diverged regions of the 16S rRNA gene and an oligonucleotide probe for the development of a PCR-liquid hybridizat...

journal_title：Journal of clinical microbiology

authors： Battles JK,Williamson JC,Pike KM,Gorelick PL,Ward JM,Gonda MA

更新日期：1995-05-01 00:00:00

abstract：:A novel lateral flow immunochromatographic device (LFD) was evaluated in several veterinary diagnostic laboratories. It was confirmed to be specific for Mycobacterium bovis and M.caprae cells. The performance of the novel LFD was assessed relative to the confirmatory tests routinely applied after culture (spoligotypin...

journal_title：Journal of clinical microbiology

authors： Stewart LD,McCallan L,McNair J,McGoldrick A,Morris R,Moyen JL,De Juan Ferré L,Romero B,Alonso E,Parsons SDC,Van Helden P,Araújo FR,Grant IR

更新日期：2017-12-01 00:00:00

abstract：:The development of diagnostic susceptibility tests for CEM-101, a new fluoroketolide, was addressed by structured studies to determine the optimal disk diffusion test concentration and effects of various testing conditions or supplements and to establish the quality control (QC) ranges for reference broth microdilutio...

journal_title：Journal of clinical microbiology

authors： Jones RN,Ross JE,Rhomberg PR,Quality Control Working Group.

更新日期：2010-04-01 00:00:00

abstract：:The group B streptococcus (GBS) capsular polysaccharide (CPS) is an important virulence factor which is also used for GBS typing. There are 10 CPS types (Ia, Ib, and II to IX). GBS that do not phenotypically type are considered nontypeable. All genes required for CPS synthesis are found on the GBS cps operon, which co...

journal_title：Journal of clinical microbiology

authors： Alhhazmi A,Pandey A,Tyrrell GJ

更新日期：2017-09-01 00:00:00

abstract：:Dried blood spot (DBS) specimens were assessed as an alternative to plasma for human immunodeficiency virus type 1 (HIV-1) serotyping by V3 loop peptide enzyme immunoassay. Nested PCR capable of distinguishing HIV-1 subtypes B and E was used as the reference standard. Ninety-two percent of DBS samples were typeable as...

journal_title：Journal of clinical microbiology

authors： Chanbancherd P,Brown AE,Trichavaroj R,Tienamporn P,Puthakird P,Limpairojn N,VanCott TC,de Souza MS

更新日期：1999-03-01 00:00:00

abstract：:A new PCR-reverse dot blot hybridization (RDBH) assay was developed for the rapid identification of Mycobacterium species in clinical isolates. The assay, which targets the 16S rRNA, was evaluated for 27 mycobacterial reference strains and 340 clinical isolates that were simultaneously identified by DNA sequencing and...

journal_title：Journal of clinical microbiology

authors： Wu X,Zhang J,Liang J,Lu Y,Li H,Li C,Yue J,Zhang L,Liu Z

更新日期：2007-06-01 00:00:00