Increasing incidence of candidemia: results from a 20-year nationwide study in Iceland.

abstract：:Timely diagnosis of respiratory syncytial virus (RSV) infection is critical for appropriate treatment of lower respiratory infection in young children. To facilitate diagnosis, we developed a rapid, specific, and sensitive TaqMan PCR method for detection of RSV A and RSV B. Two sets of primer-probe pairs were selected...

journal_title：Journal of clinical microbiology

authors： Hu A,Colella M,Tam JS,Rappaport R,Cheng SM

更新日期：2003-01-01 00:00:00

abstract：:We report a case of Veillonella parvula lumbar discitis and secondary bacteremia confirmed by molecular characterization of the 16S rRNA genes. Identification of the organism was essential for an appropriate choice of antimicrobial therapy following the failure of empirical flucloxacillin. Veillonella spp. are normal ...

journal_title：Journal of clinical microbiology

authors： Marriott D,Stark D,Harkness J

更新日期：2007-02-01 00:00:00

abstract：:In a survey of bloodstream infection (BSI) isolates across the continental United States, 162 Candida albicans isolates were fingerprinted with the species-specific probe Ca3 and the patterns were analyzed for relatedness with a computer-assisted system. The results demonstrate that particular BSI strains are more hig...

journal_title：Journal of clinical microbiology

authors： Pfaller MA,Lockhart SR,Pujol C,Swails-Wenger JA,Messer SA,Edmond MB,Jones RN,Wenzel RP,Soll DR

更新日期：1998-06-01 00:00:00

abstract：:A new urease-based enzyme-linked immunosorbent assay utilizing novel monoclonal antibodies was evaluated for the culture confirmation of Neisseria gonorrhoeae, with 270 isolates of N. gonorrhoeae, 56 isolates of diverse Neisseria spp., and 29 Moraxella isolates. The test was highly specific (100.00%) and sensitive (97...

journal_title：Journal of clinical microbiology

authors： Carballo M,Dillon JR,Lussier M,Milthorp P,Winston S,Brodeur B

更新日期：1992-08-01 00:00:00

abstract：:Infection is an important complication in patients with hematologic malignancies or solid tumors undergoing intensive cytotoxic chemotherapy. In only 20 to 30% of the febrile neutropenic episodes, an infectious agent is detected by conventional cultures. In this prospective study, the performance of broad-range PCR co...

journal_title：Journal of clinical microbiology

authors： Desmet S,Maertens J,Bueselinck K,Lagrou K

更新日期：2016-10-01 00:00:00

abstract：:We compared the BD MAX enteric bacterial panel (EBP) to culture for the detection of Salmonella, Shigella, Campylobacter, and Shiga toxin-producing enterohemorrhagic Escherichia coli (EHEC) O157 in seeded stool samples. The EBP panel demonstrated superior sensitivity and reliably detected Salmonella, EHEC O157, Shigel...

journal_title：Journal of clinical microbiology

authors： Anderson NW,Buchan BW,Ledeboer NA

更新日期：2014-04-01 00:00:00

abstract：:Thirty acquired immunodeficiency syndrome patients with mycobacterial bacteremia documented by Du Pont Isolator (Du Pont Co., Wilmington, Del.) blood cultures underwent microscopic examination of buffy coat blood smears. Of 30 patients, 14 were culture positive for Mycobacterium avium-Mycobacterium intracellulare comp...

journal_title：Journal of clinical microbiology

authors： Eng RH,Bishburg E,Smith SM,Mangia A

更新日期：1989-04-01 00:00:00

abstract：:A newly developed enzyme immunoassay (EIA) was used to detect the presence of pneumococcal teichoic and lipoteichoic acids in cerebrospinal fluid (CSF) from patients with Streptococcus pneumoniae meningitis who were being treated with antibiotics. All initial CSF samples, which on culture grew S. pneumoniae, were posi...

journal_title：Journal of clinical microbiology

authors： Stuertz K,Merx I,Eiffert H,Schmutzhard E,Mäder M,Nau R

更新日期：1998-08-01 00:00:00

abstract：:Several instruments for automated or semiautomated susceptibility testing are currently available. Three of these instruments, Autobac (General Diagnostics, Warner-Lambert Co., Morris Plains, N.J.), MS-2 (Abbott Laboratories, Dallas, Tex.), and AutoMicrobic system (AMS) (Vitek, Inc., Hazelwood, Mo.) were compared for ...

journal_title：Journal of clinical microbiology

authors： Kelly MT,Latimer JM,Balfour LC

更新日期：1982-05-01 00:00:00

abstract：:Classifications of the Campylobacter fetus subspecies fetus and venerealis were first described in 1959 and were based on the source of isolation (intestinal versus genital) and the ability of the strains to proliferate in the genital tract of cows. Two phenotypic assays (1% glycine tolerance and H2S production) were ...

journal_title：Journal of clinical microbiology

authors： van der Graaf-van Bloois L,Miller WG,Yee E,Rijnsburger M,Wagenaar JA,Duim B

更新日期：2014-12-01 00:00:00

abstract：:A dot immunobinding assay was used to detect antibodies to measles virus, mumps virus, and rubella virus antigens. Filter paper soaked with serum or whole blood was directly applied to the antigen-coated nitrocellulose sheets. The test was easy to perform, and its results agreed very well with those obtained by standa...

journal_title：Journal of clinical microbiology

authors： Condorelli F,Ziegler T

更新日期：1993-03-01 00:00:00

abstract：:To differentiate the Borrelia burgdorferi sensu lato genospecies, LightCycler real-time PCR was used for the fluorescence (SYBR Green I) melting curve analysis of borrelial recA gene PCR products. The specific melting temperature analyzed is a function of the GC/AT ratio, length, and nucleotide sequence of the amplifi...

journal_title：Journal of clinical microbiology

authors： Pietilä J,He Q,Oksi J,Viljanen MK

更新日期：2000-07-01 00:00:00

abstract：:For a positive coagulase test with certain isolates of Staphylococcus aureus, inoculation of 0.5 ml of coagulase plasma with at least two colonies and 24 h of incubation at 35 to 37 degrees C may be required. S. aureus does not multiply in coagulase plasma. There is also lot-to-lot variation in the sensitivity of coag...

journal_title：Journal of clinical microbiology

authors： Selepak ST,Witebsky FG

更新日期：1985-11-01 00:00:00

abstract：:Syrian hamsters were shown to be susceptible to infection by the Lyme disease spirochete Borrelia burgdorferi. Although these spirochetes did not cause any outward signs of illness in these animals, they did cause a generalized infection. Spirochetemia was present during the first 6 days of infection. At 14 days posti...

journal_title：Journal of clinical microbiology

authors： Johnson RC,Marek N,Kodner C

更新日期：1984-12-01 00:00:00

abstract：:Using PCR-based denaturing gradient gel electrophoresis analyses of oral bacterial samples in 20 mother-child dyads, this study demonstrated a high degree of similarity of bacterial compositions between the mothers and their children; the two may share as much as 94% of their oral bacterial spectra, including cariogen...

journal_title：Journal of clinical microbiology

authors： Li Y,Ismail AI,Ge Y,Tellez M,Sohn W

更新日期：2007-09-01 00:00:00

abstract：:The genes encoding the 56-kDa polypeptides were amplified by polymerase chain reaction from the genomic DNAs of three serotypes of Rickettsia tsutsugamushi, Gilliam, Karp, and Boryong. The amplified products were cloned into expression vector pIH821, and the recombinant antigens were expressed in Escherichia coli as f...

journal_title：Journal of clinical microbiology

authors： Kim IS,Seong SY,Woo SG,Choi MS,Kang JS,Chang WH

更新日期：1993-08-01 00:00:00

abstract：:A surveillance network was implemented by the Istituto Superiore di Sanità of Rome in collaboration with laboratories of virology in Czech Republic, Slovenia, Croatia, Albania, and Bulgaria. About 1,500 rotavirus-positive stool samples were collected from children with severe gastroenteritis admitted to hospitals or o...

journal_title：Journal of clinical microbiology

authors： Tcheremenskaia O,Marucci G,De Petris S,Ruggeri FM,Dovecar D,Sternak SL,Matyasova I,Dhimolea MK,Mladenova Z,Fiore L,Rotavirus Study Group.

更新日期：2007-07-01 00:00:00

abstract：:Bordetella pertussis is a Gram-negative bacterium that causes respiratory infections in humans. Ongoing molecular surveillance of B. pertussis acellular vaccine (aP) antigens is critical for understanding the interaction between evolutionary pressures, disease pathogenesis, and vaccine effectiveness. Methods currently...

journal_title：Journal of clinical microbiology

authors： Marchand-Austin A,Tsang RSW,Guthrie JL,Ma JH,Lim GH,Crowcroft NS,Deeks SL,Farrell DJ,Jamieson FB

更新日期：2017-05-01 00:00:00

abstract：:Recently, we reported that the patterns of antibodies to Helicobacter pylori protein antigens in serum may be useful for screening patients at high risk for ulcers (P. Aucher et al., J. Clin. Microbiol. 36:931-936, 1998). Here we report the identification, by a combination of electrophoretic, immunochemical, and prote...

journal_title：Journal of clinical microbiology

authors： Atanassov C,Pezennec L,d'Alayer J,Grollier G,Picard B,Fauchère JL

更新日期：2002-02-01 00:00:00

abstract：:Used peritoneal dialysis fluid was collected from patients undergoing continuous ambulatory peritoneal dialysis, and its pH and composition were assessed after incubation in either air or air with 5% CO2. Precipitation of calcium, magnesium, phosphate, and proteins occurred in the dialysis fluid incubated in air at 37...

journal_title：Journal of clinical microbiology

authors： Wilcox MH,Smith DG,Evans JA,Denyer SP,Finch RG,Williams P

更新日期：1990-10-01 00:00:00

abstract：:A new compound, indoxyl-beta-D-glucuronide, was assessed as a substrate for the rapid detection of Escherichia coli in urine. Incorporation of this compound into MacConkey agar allowed the direct differentiation of E. coli as deep blue colonies distinct from lactose and nonlactose fermenters. The sensitivity was 88 to...

journal_title：Journal of clinical microbiology

authors： Delisle GJ,Ley A

更新日期：1989-04-01 00:00:00

abstract：:Diagnostic use of a direct fluorescent-antibody test for detection of Campylobacter jejuni and C. coli in human fecal specimens (n = 497) was compared with detection by culturing (specificity, 99.7%; sensitivity, 40%). Conjugates were prepared from immunoglobulin G antibody against 22 Lior C. jejuni and C. coli refere...

journal_title：Journal of clinical microbiology

authors： Hodge DS,Prescott JF,Shewen PE

更新日期：1986-11-01 00:00:00

abstract：:A commercially available endotoxin assay (CS-TAL) employing a chromogenic peptide and an amebocyte lysate from the Japanese horseshoe crab, Tachypleus tridentatus, gave a positive result with aqueous extracts of all 15 strains of Candida albicans and 1 strain each of Candida tropicalis, Cryptococcus neoformans, and a ...

journal_title：Journal of clinical microbiology

authors： Hodes DS,Heon D,Hass A,Hyatt AC,Hodes HL

更新日期：1987-09-01 00:00:00

abstract：:The capsular-like envelope of Legionella pneumophila strains Togus 1 (serotype 2) and Philadelphia 1 (serotype 1) was isolated and purified by column chromatography on Sepharose 6B. Antibody raised in rabbits to these two antigenic materials did not cross-react in gel diffusion. Upon electrophoresis followed by gel di...

journal_title：Journal of clinical microbiology

authors： Smith RA,DiGiorgio S,Darner J,Wilhelm A

更新日期：1981-04-01 00:00:00

abstract：:Plasmid-mediated colistin resistance (PMCR) is a global public health concern, given its ease of transmissibility. The purpose of this study was to evaluate two methods for the detection of PMCR from bacterial colonies: (i) the NG-Test MCR-1 lateral flow immunoassay (LFA; NG Biotech, Guipry, France) and (ii) the EDTA-...

journal_title：Journal of clinical microbiology

authors： Fenwick AJ,Bergman Y,Lewis S,Yee R,Uhlemann AC,Cole N,Kohner P,Ordak C,Green DA,Schuetz AN,Humphries R,Simner PJ

更新日期：2020-03-25 00:00:00

abstract：:The attenuated bacillus Calmette-Guérin (BCG) vaccine strain is derived from a virulent strain of Mycobacterium bovis. BCG is difficult to differentiate from other strains of M. bovis and other members of the M. tuberculosis complex by conventional methods. Recently, a genomic region designated RD1 was found to be pre...

journal_title：Journal of clinical microbiology

authors： Talbot EA,Williams DL,Frothingham R

更新日期：1997-03-01 00:00:00

abstract：:Pulmonary infection with Pseudomonas aeruginosa is a major cause of morbidity and mortality in cystic fibrosis (CF) patients. P. aeruginosa toxin is one of several proposed virulence factors which may be responsible for chronic P. aeruginosa infections in these patients. With a highly specific, sensitive, and quantita...

journal_title：Journal of clinical microbiology

authors： Cukor G,Blacklow NR,Nowak NA,Rich CM,Braverman LE,Fischer RA

更新日期：1983-09-01 00:00:00

abstract：:Automation of the clinical microbiology laboratory has become more prominent as laboratories face higher specimen volumes and understaffing and are becoming more consolidated. One recent advancement is the use of digital image analysis to rapidly distinguish between chromogenic growth for screening bacterial cultures....

journal_title：Journal of clinical microbiology

authors： Faron ML,Buchan BW,Relich RF,Clark J,Ledeboer NA

更新日期：2020-03-25 00:00:00

abstract：:Mycoplasma hominis most frequently causes diseases of the genitourinary tract. Extragenital infections are uncommon, with almost all occurring in immunosuppressed persons or those predisposed due to trauma or surgery. We present the case of a previously well man who developed an M. hominis-associated parapharyngeal ab...

journal_title：Journal of clinical microbiology

authors： Kennedy KJ,Prince S,Makeham T

更新日期：2009-09-01 00:00:00

abstract：:Since the discovery of the Norwalk virus (NV) by immune electron microscopy (IEM) in 1972, serologic studies with this virus have relied on particle-positive fecal material from infected volunteers as the source of antigen because it has not been possible to propagate this virus in cell culture. However, the recent cl...

journal_title：Journal of clinical microbiology

authors： Green KY,Lew JF,Jiang X,Kapikian AZ,Estes MK

更新日期：1993-08-01 00:00:00