Hospital specificity, region specificity, and fluconazole resistance of Candida albicans bloodstream isolates.

abstract：:Blood culture bottles with antimicrobial removal systems are recommended for patients who develop fever while on antibiotics. This study compared the ability of Becton Dickinson (Sparks, MD) BACTEC PLUS bottles and bioMerieux (Durham, NC) BacT/Alert FA bottles to effectively remove vancomycin, cefoxitin, ceftriaxone, ...

journal_title：Journal of clinical microbiology

authors： Flayhart D,Borek AP,Wakefield T,Dick J,Carroll KC

更新日期：2007-03-01 00:00:00

abstract：:Public health microbiology laboratories (PHLs) are on the cusp of unprecedented improvements in pathogen identification, antibiotic resistance detection, and outbreak investigation by using whole-genome sequencing (WGS). However, considerable challenges remain due to the lack of common standards. Here, we describe the...

journal_title：Journal of clinical microbiology

authors： Kozyreva VK,Truong CL,Greninger AL,Crandall J,Mukhopadhyay R,Chaturvedi V

更新日期：2017-08-01 00:00:00

abstract：:Mycobacterium tuberculosis isolates were studied by comparing chromosomal DNA fingerprint patterns produced by digestion of chromosomal DNA with BamHI, followed by agarose electrophoresis and hybridization with radiolabeled probes of insertion sequence IS6110. DNA fingerprints of 14 isolates from separate members of f...

journal_title：Journal of clinical microbiology

authors： Mazurek GH,Cave MD,Eisenach KD,Wallace RJ Jr,Bates JH,Crawford JT

更新日期：1991-09-01 00:00:00

abstract：:The in vitro activities of 17 antifungal drugs against a panel of 20 dermatophytes comprising 6 different species were determined using a microdilution assay according to the NCCLS M38-P method with some modifications. Terbinafine was the most potent systemic drug while tolnaftate and amorolfine were the most active t...

journal_title：Journal of clinical microbiology

authors： Favre B,Hofbauer B,Hildering KS,Ryder NS

更新日期：2003-10-01 00:00:00

abstract：:We evaluated the Prodesse ProFlu-1 real-time reverse transcription-PCR multiplex assay with the SmartCycler instrument for the detection of human respiratory syncytial virus (RSV) and influenza A and B viruses in comparison to conventional cell culture and antigen immunoassays with the BD Directigen A+B and Binax NOW ...

journal_title：Journal of clinical microbiology

authors： Liao RS,Tomalty LL,Majury A,Zoutman DE

更新日期：2009-03-01 00:00:00

abstract：:Ceftolozane-tazobactam (C/T) is a novel beta-lactam-beta-lactamase inhibitor combination antibiotic approved by the U.S. Food and Drug Administration in 2014 for the treatment of complicated intra-abdominal infections (in combination with metronidazole) and complicated urinary tract infections. In this study, we evalu...

journal_title：Journal of clinical microbiology

authors： Bailey AL,Armstrong T,Dwivedi HP,Denys GA,Hindler J,Campeau S,Traczewski M,Humphries R,Burnham CA

更新日期：2018-08-27 00:00:00

abstract：:The results of the application of a range of typing procedures to the identification and classification of 6,264 cultures of nontuberculous mycobacteria from human sources and the environment are reported. Seroagglutination, an enzyme-linked immunosorbent assay applied to whole bacteria or the glycolipid typing antige...

journal_title：Journal of clinical microbiology

authors： Tsang AY,Denner JC,Brennan PJ,McClatchy JK

更新日期：1992-02-01 00:00:00

abstract：:This study compares the identification, typing, and phylogenetic relationships of the most prevalent clinical Nocardia species in Spain, as determined via sequence analysis of their housekeeping genes gyrB and rpoB, with the results returned by the gold standard 16S rRNA method. gyrB and rpoB analyses identified Nocar...

journal_title：Journal of clinical microbiology

authors： Carrasco G,Valdezate S,Garrido N,Villalón P,Medina-Pascual MJ,Sáez-Nieto JA

更新日期：2013-11-01 00:00:00

abstract：:The DNA hybridization assay for genes encoding for Escherichia coli enterotoxins was used to examine water specimens in Thailand. In a reconstruction experiment, the DNA hybridization assay was 10(4) times more sensitive than testing random E. coli in the Y-1 adrenal and suckling mouse assays in identifying enterotoxi...

journal_title：Journal of clinical microbiology

authors： Echeverria P,Seriwatana J,Chityothin O,Chaicumpa W,Tirapat C

更新日期：1982-12-01 00:00:00

abstract：:A space-saving media table designed to facilitate pouring large quantities of plated media in a small area is described. ...

journal_title：Journal of clinical microbiology

authors： McGonagle LA,Schmidt G,Eng S,Wirsing K

更新日期：1975-07-01 00:00:00

abstract：:Hepatitis B surface antigen was concentrated and purified from plasma by two simple steps of purification. In the first step the antigen was purified 24-fold by polyethylene glycol precipitation. An additional 10-fold purification was achieved by batchwise adsorption to hydroxylapatite and subsequent elution with 0.02...

journal_title：Journal of clinical microbiology

authors： Vnek J,Prince AM

更新日期：1976-06-01 00:00:00

abstract：:Standard culture methods for diagnosis of Streptococcus pneumoniae pneumonia take at least 24 h. The BinaxNOW urine-based test for S. pneumoniae (BinaxNOW-SP) takes only 15 min to conduct, potentially enabling earlier diagnosis and targeted treatment. This study was conducted to assess whether the use of BinaxNOW-SP a...

journal_title：Journal of clinical microbiology

authors： Sinclair A,Xie X,Teltscher M,Dendukuri N

更新日期：2013-07-01 00:00:00

abstract：:Indirect and double-antibody sandwich enzyme-linked immunosorbent assay techniques were compared for serotyping 42 herpes simplex virus isolates. Both procedures gave similar results, which were in complete agreement with those obtained by inhibition of the indirect hemagglutination test. The indirect technique proved...

journal_title：Journal of clinical microbiology

authors： Gerna G,Battaglia M,Revello MG,Gerna MT

更新日期：1983-05-01 00:00:00

abstract：:The polymerase chain reaction was used to detect human herpesvirus 6 (HHV-6) DNA in peripheral blood mononuclear cells from patients with exanthem subitum. Amplified products were detected by agarose gel electrophoresis and dot blot hybridization with a cloned DNA probe. No cross-hybridization with DNAs of five other ...

journal_title：Journal of clinical microbiology

authors： Kondo K,Hayakawa Y,Mori H,Sato S,Kondo T,Takahashi K,Minamishima Y,Takahashi M,Yamanishi K

更新日期：1990-05-01 00:00:00

abstract：:Clostridium difficile is now recognized as the major agent responsible for nosocomial diarrhea in adults. Among the genotyping methods available, arbitrarily primed PCR (AP-PCR), PCR-ribotyping, and pulsed-field gel electrophoresis (PFGE) have been widely used for investigating outbreaks of C. difficile infections. Ho...

journal_title：Journal of clinical microbiology

authors： Bidet P,Lalande V,Salauze B,Burghoffer B,Avesani V,Delmée M,Rossier A,Barbut F,Petit JC

更新日期：2000-07-01 00:00:00

abstract：:The causative agent of Johne's disease is Mycobacterium avium subsp. paratuberculosis. This is a chronic, debilitating gastrointestinal disorder that affects ruminants and is responsible for significant economic loss. The specimen processing method that combines C(18)-carboxypropylbetaine (CB-18) treatment and lytic e...

journal_title：Journal of clinical microbiology

authors： Thornton CG,MacLellan KM,Stabel JR,Carothers C,Whitlock RH,Passen S

更新日期：2002-05-01 00:00:00

abstract：:Therapeutic options for infections caused by gram-negative organisms expressing plasmid-mediated AmpC beta-lactamases are limited because these organisms are usually resistant to all the beta-lactam antibiotics, except for cefepime, cefpirome, and the carbapenems. These organisms are a major concern in nosocomial infe...

journal_title：Journal of clinical microbiology

authors： Pérez-Pérez FJ,Hanson ND

更新日期：2002-06-01 00:00:00

abstract：:Four commercial kits, Oxi/Ferm (OF), API 20E (AP), Minitek (MT; BBL Microbiology Systems), and Flow N/F (NF), were evaluated, without additional tests, for identification of 258 gram-negative nonfermentative rods. OF and MT were read after 48 h of incubation, and AP and NF were read after both 24 and 48 h of incubatio...

journal_title：Journal of clinical microbiology

authors： Appelbaum PC,Stavitz J,Bentz MS,von Kuster LC

更新日期：1980-08-01 00:00:00

abstract：:A recombinant protein (p41-G) of an antigenic region of flagellin was used in a standard and amplified enzyme-linked immunosorbent assay (ELISA) to detect antibodies to Borrelia burgdorferi, the causative agent of Lyme borreliosis. Comparable sensitivities (88 to 94%) were noted when sera from 17 persons who had eryth...

journal_title：Journal of clinical microbiology

authors： Magnarelli LA,Fikrig E,Berland R,Anderson JF,Flavell RA

更新日期：1992-12-01 00:00:00

abstract：:Antigens of Rhodococcus equi were analyzed by immunoblotting with naturally infected foal sera. Immunoblots of whole-cell antigen preparations of clinical isolates of R. equi revealed that major protein bands with molecular masses of 15 to 17 kDa were present in all clinical isolates tested and all isolates virulent f...

journal_title：Journal of clinical microbiology

authors： Takai S,Koike K,Ohbushi S,Izumi C,Tsubaki S

更新日期：1991-03-01 00:00:00

abstract：:A new method for determining the MICs for Aspergillus species with slant-shaking incubation is described. The method was rapid (24 h), produced sharp visual end points, and showed high reproducibility. Additionally, MICs measured by this method correlated well with, as well as were two- to sixfold lower than, those by...

journal_title：Journal of clinical microbiology

authors： Yamada H,Kohno S,Maesaki S,Koga H,Kaku M,Hara K,Tanaka H

更新日期：1993-04-01 00:00:00

abstract：:PCR-restriction fragment length polymorphism analysis (PRA) of the hsp65 gene (A. Telenti, F. Marchesi, M. Balz, F. Bally, E. C. Böttger, and T. Bodmer, J. Clin. Microbiol. 31:175-178, 1993) was applied to 108 mycobacterial isolates representing 34 species to evaluate its potential as a rapid reference method. A total...

journal_title：Journal of clinical microbiology

authors： Devallois A,Goh KS,Rastogi N

更新日期：1997-11-01 00:00:00

abstract：:Yarrowia lipolytica is a weakly pathogenic yeast that is rarely isolated from the blood. We observed transient recurrent catheter-related fungemia attributable to this organism in a leukemic patient. The fungemia and accompanying fever subsided spontaneously. The data suggest that it might be possible to withhold spec...

journal_title：Journal of clinical microbiology

authors： Chang CL,Park TH,Lee EY,Lim YT,Son HC

更新日期：2001-03-01 00:00:00

abstract：:We determined the types of cholera toxin (CT) produced by a collection of 185 Vibrio cholerae O1 strains isolated in Bangladesh over the past 45 years. All of the El Tor strains of V. cholerae O1 isolated since 2001 produced CT of the classical biotype, while those isolated before 2001 produced CT of the El Tor biotyp...

journal_title：Journal of clinical microbiology

authors： Nair GB,Qadri F,Holmgren J,Svennerholm AM,Safa A,Bhuiyan NA,Ahmad QS,Faruque SM,Faruque AS,Takeda Y,Sack DA

更新日期：2006-11-01 00:00:00

abstract：:We have introduced a time-resolved fluorometry (TRF)-based microwell hybridization assay for PCR products in detection of herpes simplex virus (HSV) in cerebrospinal fluid (CSF) specimens. TRF is a sensitive nonradioactive detection technique which involves the use of lanthanide chelates as fluorescent labels. We used...

journal_title：Journal of clinical microbiology

authors： Hukkanen V,Rehn T,Kajander R,Sjöroos M,Waris M

更新日期：2000-09-01 00:00:00

abstract：:A case of tick-borne encephalitis (TBE) has not been reported for many years in Japan, although a serological survey of sera from domestic animals suggested the presence of TBE foci in Hokkaido, the northern island of Japan. Studies were conducted to prove the presence of an endemic focus of TBE virus in Japan by mean...

journal_title：Journal of clinical microbiology

authors： Takashima I,Morita K,Chiba M,Hayasaka D,Sato T,Takezawa C,Igarashi A,Kariwa H,Yoshimatsu K,Arikawa J,Hashimoto N

更新日期：1997-08-01 00:00:00

abstract：:Many commercially available media for cultivation of mycobacteria have failed to support the growth of these organisms. This is especially true of media prepared with albumin-containing enrichments. Earlier, we developed a method for rapid identification of good albumin enrichments for agar-based media used to test th...

journal_title：Journal of clinical microbiology

authors： Butler WR,Warren NG,Kubica GP,Kilburn JO

更新日期：1990-05-01 00:00:00

abstract：:The effectiveness of antiretroviral therapy may be limited by the development of human immunodeficiency virus type 1 (HIV-1) resistance. Monitoring for resistance will perhaps allow changes in therapy prior to deterioration in the patient's clinical or immunologic status. Our objective was to develop a rapid, specific...

journal_title：Journal of clinical microbiology

authors： Frenkel LM,Wagner LE 2nd,Atwood SM,Cummins TJ,Dewhurst S

更新日期：1995-02-01 00:00:00

abstract：:The new anaerobe and Corynebacterium (ANC) identification card for Vitek 2 was compared with a 16S rRNA gene sequencing (16S) reference method for accuracy in the identification of corynebacteria and anaerobic species. Testing was performed on a Vitek 2 XL system with modified software at three clinical trial laborato...

journal_title：Journal of clinical microbiology

authors： Rennie RP,Brosnikoff C,Turnbull L,Reller LB,Mirrett S,Janda W,Ristow K,Krilcich A

更新日期：2008-08-01 00:00:00

abstract：:A new direct immunofluorescence monoclonal antibody (DFA) method (Genetic Systems, Inc., Seattle, Wash.) for identification of Pneumocystis carinii in induced sputum and bronchoalveolar lavage specimens was compared in a blinded study with an established Giemsa stain method. We evaluated 148 consecutive clinical speci...

journal_title：Journal of clinical microbiology

authors： Wolfson JS,Waldron MA,Sierra LS

更新日期：1990-09-01 00:00:00