Specific, sensitive, and rapid assay for human immunodeficiency virus type 1 pol mutations associated with resistance to zidovudine and didanosine.

abstract：:Invasive pulmonary aspergillosis (IPA) is a frequently fatal infection in immunocompromised patients that is difficult to diagnose. Present methods for detection of Aspergillus spp. in bronchoalveolar lavage (BAL) fluid and in tissue vary in sensitivity and specificity. We therefore developed an A. fumigatus-specific ...

journal_title：Journal of clinical microbiology

authors： O'Sullivan CE,Kasai M,Francesconi A,Petraitis V,Petraitiene R,Kelaher AM,Sarafandi AA,Walsh TJ

更新日期：2003-12-01 00:00:00

abstract：:Routine bacterial cultures of corneal scrapings from seven cats with either ulcerative feline keratitis, keratomalacia, or both yielded colonies which were identified by 16S rRNA gene sequencing as Mycoplasma felis (six cases) and Mycoplasma gateae (one case). Identification of the pathogens allowed the use of less em...

journal_title：Journal of clinical microbiology

authors： Gray LD,Ketring KL,Tang YW

更新日期：2005-07-01 00:00:00

abstract：:Restriction endonucleases BamHI, BclI, BglI, BglII, BstEII, EcoRI, HindIII, HpaI, SalI, SmalI, XbalI, and XholI were used to analyze 61 selected strains of adenovirus type 3 (Ad3) isolated from Africa, Asia, Australia, Europe, North America, and South America. It was noted that the use of BamHI, BclI, BglII, HpaI, Sal...

journal_title：Journal of clinical microbiology

authors： Li QG,Wadell G

更新日期：1988-05-01 00:00:00

abstract：:A simple method of storage and retrieval of serum samples for adenovirus serology is described. Complement-fixing antibodies remained stable for at least 5 years when stored at -10 degrees C or below. ...

journal_title：Journal of clinical microbiology

authors： Edwards EA

更新日期：1977-02-01 00:00:00

abstract：:During the 2009 H1N1 influenza A outbreak, 773 children were tested for influenza by direct fluorescent-antibody testing with PCR confirmation. Direct fluorescent-antibody testing has a specificity of 99.6% but a sensitivity of only 65.0%. Physicians should recognize diagnostic limitations of direct fluorescent-antibo...

journal_title：Journal of clinical microbiology

authors： Bakerman P,Balasuriya L,Fried O,Tellez D,Garcia-Filion P,Dalton H

更新日期：2011-10-01 00:00:00

abstract：:Of 4,000 endocervical specimens tested with the Chlamydiazyme enzyme-linked immunosorbent assay (Abbott Laboratories), 233 (5.8%) gave positive results (A492 above the cutoff), which were confirmed with a blocking reagent (Abbott). An additional 34 specimens (14.6%) with chlamydial antigen were detected and confirmed ...

journal_title：Journal of clinical microbiology

authors： Kellogg JA,Seiple JW,Stroll ES

更新日期：1993-06-01 00:00:00

abstract：:A method for extracting poliovirus (PV) from stool extracts was developed. Magnetic nanoparticles sensitized with soluble PV receptor efficiently extracted PV pseudovirus (>99% extraction) or endogenous infectious PVs (>90% extraction) from stool extracts. This method would be useful for extraction of PV from crude bi...

journal_title：Journal of clinical microbiology

authors： Arita M

更新日期：2013-08-01 00:00:00

abstract：:Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry with a Bruker Daltonics microflex LT system was applied to 90 well-characterized catalase-negative, Gram-positive cocci not belonging to the streptococci or enterococci. Biotyper version 2.0.43.1 software was used singly or in com...

journal_title：Journal of clinical microbiology

authors： Christensen JJ,Dargis R,Hammer M,Justesen US,Nielsen XC,Kemp M,Danish MALDI-TOF MS Study Group.

更新日期：2012-05-01 00:00:00

abstract：:Metarhizium viride has been associated with fatal systemic mycoses in chameleons, but subsequent data on mycoses caused by this fungus in reptiles are lacking. The aim of this investigation was therefore to obtain information on the presence of M. viride in reptiles kept as pets in captivity and its association with c...

journal_title：Journal of clinical microbiology

authors： Schmidt V,Klasen L,Schneider J,Hübel J,Pees M

更新日期：2017-03-01 00:00:00

abstract：:A cluster of methicillin-resistant Staphylococcus aureus (MRSA) infections among patients on an intensive care unit (ICU) was detected by routine infection control surveillance. In the period from 5 January to 22 June 1995, 10 patients on the ICU and a further 6 patients (5 on one ward that had received colonized pati...

journal_title：Journal of clinical microbiology

authors： Tambic A,Power EG,Talsania H,Anthony RM,French GL

更新日期：1997-12-01 00:00:00

abstract：:The BACTEC NR660 blood culture system, which uses infrared spectroscopy to detect carbon dioxide generated by bacterial growth, was compared with the new medium formulation of the Oxoid Signal system. Two trials were conducted: a comparative study of 88 organisms in simulated blood cultures and a clinical trial of 3,3...

journal_title：Journal of clinical microbiology

authors： Daley C,Lim I,Modra J,Wilkinson I

更新日期：1990-07-01 00:00:00

abstract：:We describe a simple method for the rapid detection of bovine viral diarrhea virus (BVDV) that uses a one-tube reverse transcription PCR (RT-PCR) and total RNA extracted directly from a variety of bovine specimens, including whole blood and tissues. Reagents for both RT and PCR were combined in a one-tube, single-buff...

journal_title：Journal of clinical microbiology

authors： Hamel AL,Wasylyshen MD,Nayar GP

更新日期：1995-02-01 00:00:00

abstract：:Serial cerebrospinal fluid samples were obtained from 14 multiple sclerosis patients over a period of several months and were tested under code for Inoue-Melnick virus. In five of the positive patients, virus was present in 28 of 32 specimens collected over a period of 2 to 5 months. Four patients, from whom a total o...

journal_title：Journal of clinical microbiology

authors： Melnick JL,Seidel E,Wang SS,Muchinik G,Rashti EJ,Jacobs LD,Freeman AI,O'Malley J

更新日期：1985-10-01 00:00:00

abstract：:A total of 513 blood specimens, predominantly from organ transplant recipients, human immunodeficiency virus-positive patients, and bone marrow transplant recipients, were tested for cytomegalovirus (CMV) by culture and pp65 antigenemia across four test sites. Peripheral blood leukocytes were examined by using both th...

journal_title：Journal of clinical microbiology

authors： St George K,Boyd MJ,Lipson SM,Ferguson D,Cartmell GF,Falk LH,Rinaldo CR,Landry ML

更新日期：2000-04-01 00:00:00

abstract：:Disseminated Mycobacterium avium infection is a common complication in late-stage AIDS. We describe a patient seropositive for human immunodeficiency virus with a disseminated infection caused by mycobacteria. Acid-fast rods were visible by microscopy in stool and bone marrow and in specimens from skin, liver, spleen,...

journal_title：Journal of clinical microbiology

authors： Kirschner P,Vogel U,Hein R,Böttger EC

更新日期：1994-03-01 00:00:00

abstract：:HIV-1 non-B subtypes/circulating recombinant forms (CRFs) are increasing worldwide. Since subtype identification can be clinically relevant, we assessed the added value in HIV-1 subtyping using updated molecular phylogeny (Mphy) and the performance of routinely used automated tools. Updated Mphy (2015 updated referenc...

journal_title：Journal of clinical microbiology

authors： Fabeni L,Berno G,Fokam J,Bertoli A,Alteri C,Gori C,Forbici F,Takou D,Vergori A,Zaccarelli M,Maffongelli G,Borghi V,Latini A,Pennica A,Mastroianni CM,Montella F,Mussini C,Andreoni M,Antinori A,Perno CF,Santoro MM,

更新日期：2017-09-01 00:00:00

abstract：:An evaluation was undertaken to determine the optimal method for the in vitro susceptibility testing of 26 Nocardia asteroides complex isolates to the following antimicrobial agents: amikacin, ampicillin, amoxicillin-clavulanate, ceftriaxone, ciprofloxacin, erythromycin, imipenem, minocycline, and trimethoprim-sulfame...

journal_title：Journal of clinical microbiology

authors： Ambaye A,Kohner PC,Wollan PC,Roberts KL,Roberts GD,Cockerill FR 3rd

更新日期：1997-04-01 00:00:00

abstract：:The biosynthesis pathway for riboflavin (vitamin B(2)), the precursor of the essential cofactors flavin mononucleotide and flavin adenine dinucleotide, is present in bacteria and plants but is absent in vertebrates. Due to their conservation in bacterial species and their absence in humans, the riboflavin synthesis ge...

journal_title：Journal of clinical microbiology

authors： Bereswill S,Hinkelmann S,Kist M,Sander A

更新日期：1999-10-01 00:00:00

abstract：:The coelomycete Colletotrichum gloeosporioides was isolated in pure culture from subcutaneous nodules of the left forearm and elbow of a farmer after traumatic injury. To our knowledge, we report the first case involving this fungus as an etiological agent of subcutaneous infection. The in vitro inhibitory activities ...

journal_title：Journal of clinical microbiology

authors： Guarro J,Svidzinski TE,Zaror L,Forjaz MH,Gené J,Fischman O

更新日期：1998-10-01 00:00:00

abstract：:A modified CAMP test was used to identify 973 Vibrio cholerae isolates by phenotype. Eltor and non-O1 strains were CAMP positive; classical strains were CAMP negative. Sausage-shaped zones of hemolysis of eltor strains were easily distinguished from narrower bands of non-O1 isolates. For O1 isolates, there was 100% ag...

journal_title：Journal of clinical microbiology

authors： Lesmana M,Subekti D,Tjaniadi P,Pazzaglia G

更新日期：1994-01-01 00:00:00

abstract：:We first noted the appearance of thymidine-requiring, gram-negative bacilli in clinical specimens 2 years ago. Since then we have seen 10 patients colonized or infected with these organisms. These strains do not grow on Mueller-Hinton media, growth on MacConkey agar is variable, and growth in API 20E (Analytab Product...

journal_title：Journal of clinical microbiology

authors： King CH,Shlaes DM,Dul MJ

更新日期：1983-07-01 00:00:00

abstract：:The development of a specimen collection and transport medium outfit for the rapid laboratory diagnosis of whoping cough is described. The transport medium consisted of a semisolid agar containing charcoal, cephalexin, and defibrinated horse blood. It was also found to be an excellent enrichment medium for the selecti...

journal_title：Journal of clinical microbiology

authors： Regan J,Lowe F

更新日期：1977-09-01 00:00:00

abstract：:Two DNA probes, one 1.1- and one 0.75-kb probe, specific for Serpulina hyodysenteriae were isolated from a genomic library generated from virulent S. hyodysenteriae 5380. These probes are highly specific and react with all S. hyodysenteriae strains tested. Under stringent conditions, the DNA probes did not react with ...

journal_title：Journal of clinical microbiology

authors： Sotiropoulos C,Smith SC,Coloe PJ

更新日期：1993-07-01 00:00:00

abstract：:The bacterial genus Gordona includes seven species of mycolic acid-containing actinomycetes which are cultured from environmental sources and rarely from clinical samples. They have been implicated in primary pulmonary diseases, nosocomial wound infections, and central nervous system infections in two immunocompromise...

journal_title：Journal of clinical microbiology

authors： Drancourt M,Pelletier J,Cherif AA,Raoult D

更新日期：1997-02-01 00:00:00

abstract：:To investigate whether both tissue culture and PCR on a sequence from the repetitive rDNA could contribute to the diagnosis of toxoplasmosis, blood samples and, if they were available, cerebrospinal fluid (CSF) and aqueous humor samples from 72 human immunodeficiency virus-seropositive patients with suspected toxoplas...

journal_title：Journal of clinical microbiology

authors： Dupon M,Cazenave J,Pellegrin JL,Ragnaud JM,Cheyrou A,Fischer I,Leng B,Lacut JY

更新日期：1995-09-01 00:00:00

abstract：:We evaluated two commercial F1 antigen capture-based immunochromatographic dipsticks, Yersinia Pestis (F1) Smart II and Plague BioThreat Alert test strips, in detecting plague bacilli by using whole-blood samples from mice experimentally infected with Yersinia pestis CO92. To assess the specificities of these dipstick...

journal_title：Journal of clinical microbiology

authors： Sha J,Endsley JJ,Kirtley ML,Foltz SM,Huante MB,Erova TE,Kozlova EV,Popov VL,Yeager LA,Zudina IV,Motin VL,Peterson JW,DeBord KL,Chopra AK

更新日期：2011-05-01 00:00:00

abstract：:A procedure was developed for characterization of Chlamydia trachomatis strains by using restriction endonuclease analysis of amplified genes of the major outer membrane protein (MOMP). Reference strains of the 15 serovars (A through K and L1 through L3) and clinical isolates were tested. The nucleotide sequences of t...

journal_title：Journal of clinical microbiology

authors： Rodriguez P,Vekris A,de Barbeyrac B,Dutilh B,Bonnet J,Bebear C

更新日期：1991-06-01 00:00:00

abstract：:Simple sample-processing methods for PCR detection of Porphyromonas gingivalis, a major pathogen causing adult periodontitis, from saliva were studied. The ability to detect P. gingivalis from 118 salivary samples by PCR after boiling and Chelex 100 processing was compared with bacterial culture. P. gingivalis was det...

journal_title：Journal of clinical microbiology

authors： Mättö J,Saarela M,Alaluusua S,Oja V,Jousimies-Somer H,Asikainen S

更新日期：1998-01-01 00:00:00

abstract：:Phenolphthalein diphosphate was incorporated into a primary blood agar medium for use in performing quantitative urine cultures. Phosphatase-negative staphylococci, such as Staphylococcus saprophyticus, were differentiated from phosphatase-positive species, such as Staphylococcus epidermidis, by spot testing colonies ...

journal_title：Journal of clinical microbiology

authors： Pickett DA,Welch DF

更新日期：1985-03-01 00:00:00

abstract：:Alere i RSV is a novel rapid test which applies a nicking enzyme amplification reaction to detect respiratory syncytial virus in point-of-care settings. In this study, we evaluated the Alere i RSV assay by using frozen nasopharyngeal swab samples that were collected in viral transport medium from children hospitalized...

journal_title：Journal of clinical microbiology

authors： Peters RM,Schnee SV,Tabatabai J,Schnitzler P,Pfeil J

更新日期：2017-04-01 00:00:00