Abstract:
:A total of 513 blood specimens, predominantly from organ transplant recipients, human immunodeficiency virus-positive patients, and bone marrow transplant recipients, were tested for cytomegalovirus (CMV) by culture and pp65 antigenemia across four test sites. Peripheral blood leukocytes were examined by using both the Biotest CMV Brite and the Bartels/Argene CMV Antigenemia kits. A total of 109 specimens were positive for CMV, 106 (97%) were positive by antigenemia, and 34 (31%) were positive by culture. According to the manufacturers' instructions, 150,000 cells were applied per slide for the Biotest kit and 200,000 cells per slide for the Bartels kit. A total of 93 specimens (88%) were positive by the Biotest kit, and 86 (81%) were positive by the Bartels kit. In specimens found to be positive by only one kit, the positive cell counts were low (median, 1; range, 1 to 7). When the data from all four sites were combined and analyzed, there was no statistical difference between the performance of the two kits; the Biotest and Bartels kits were found to be equivalent in sensitivity, specificity, and positive and negative predictive values for the detection of CMV pp65 antigenemia.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
St George K,Boyd MJ,Lipson SM,Ferguson D,Cartmell GF,Falk LH,Rinaldo CR,Landry MLdoi
10.1128/JCM.38.4.1430-1433.2000subject
Has Abstractpub_date
2000-04-01 00:00:00pages
1430-3issue
4eissn
0095-1137issn
1098-660Xjournal_volume
38pub_type
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