Identification of isolates of Streptococcus canis infecting humans.

abstract：:Participants in the College of American Pathologists' Mycobacteriology E proficiency testing survey in 1993 were asked to complete a questionnaire addressing mycobacterial test methods, test volume, and frequency of detection of drug-resistant Mycobacterium tuberculosis (MTB). A similar questionnaire had been distribu...

journal_title：Journal of clinical microbiology

authors： Woods GL,Witebsky FG

更新日期：1995-02-01 00:00:00

abstract：:We analyzed the characteristics of subjects from the same area who were infected with hepatitis C virus genotypes 1 through 4 and subtypes 1a and 1b. Our data are consistent with a rapid evolution in the epidemiology of HCV genotypes and argue against different pathogenic potentials for genotypes 1b and 2. ...

journal_title：Journal of clinical microbiology

authors： Ravaggi A,Rossini A,Mazza C,Puoti M,Marin MG,Cariani E

更新日期：1996-11-01 00:00:00

abstract：:We have previously reported that the pCS20 PCR detection assay for Cowdria ruminantium, the causative agent of heartwater disease of ruminants, is more sensitive than xenodiagnosis and the pCS20 DNA probe for the detection of infection in the vector Amblyomma ticks. Here, we further assessed the reliability of the PCR...

journal_title：Journal of clinical microbiology

authors： Peter TF,Barbet AF,Alleman AR,Simbi BH,Burridge MJ,Mahan SM

更新日期：2000-04-01 00:00:00

abstract：:Parasite-specific humoral and cellular immune responses were evaluated in in nine immunosuppressed patients with Strongyloides stercoralis infection. Four patients had the disseminated form of the disease, and five had serious manifestations without evidence of hyperinfection. All patients had high levels of immunoglo...

journal_title：Journal of clinical microbiology

authors： Genta RM,Douce RW,Walzer PD

更新日期：1986-06-01 00:00:00

abstract：:This report describes the development and evaluation of an enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to Entamoeba histolytica. Highly sensitive and reproducible results were obtained in antigen-coated plates prepared by air-drying at 37 degrees C. Comparison of the ELISA with indirect f...

journal_title：Journal of clinical microbiology

authors： Yang J,Kennedy MT

更新日期：1979-12-01 00:00:00

abstract：:A total of 221 isolates of multidrug-resistant Salmonella enterica serovar Typhimurium in Japan were characterized in the present study. The results revealed that clonal serovar Typhimurium definitive phage type 104 strains prevailed and that these strains had drug resistance patterns, integron types, and pulsed-field...

journal_title：Journal of clinical microbiology

authors： Izumiya H,Terajima J,Matsushita S,Tamura K,Watanabe H

更新日期：2001-07-01 00:00:00

abstract：:A case of zygomycosis caused by Apophysomyces elegans in a patient having 25% full-thickness burns is described. Amputation of the leg was necessary to control rapid tissue invasion. The fungus was isolated from soil in the burn environment. ...

journal_title：Journal of clinical microbiology

authors： Cooter RD,Lim IS,Ellis DH,Leitch IO

更新日期：1990-09-01 00:00:00

abstract：:Approximately 15 to 35% of patients with a first episode of Clostridium difficile-associated diarrhea relapse within 2 months. Between 1994 and 1997, strains from 93 hospitalized patients with C. difficile recurrences were fingerprinted by using both serotyping and PCR-ribotyping. The results showed that 48.4% of clin...

journal_title：Journal of clinical microbiology

authors： Barbut F,Richard A,Hamadi K,Chomette V,Burghoffer B,Petit JC

更新日期：2000-06-01 00:00:00

abstract：:By serological methods, all 83 known types of Streptococcus pneumoniae could be shown to possess C polysaccharide and F antigen. Cross-reactions due to these two antigens between pneumococci and a broad range of most other commonly encountered streptococci were examined. The presence of an antigen closely similar or i...

journal_title：Journal of clinical microbiology

authors： Sørensen UB,Henrichsen J

更新日期：1987-10-01 00:00:00

abstract：:Several species within the Burkholderia cepacia complex (BCC) have emerged as significant opportunistic pathogens of patients with cystic fibrosis (CF). BCC infection is typically associated with a poor clinical prognosis and decreased survival. These factors, combined with the existence of highly transmissible epidem...

journal_title：Journal of clinical microbiology

authors： Brown AR,Govan JR

更新日期：2007-06-01 00:00:00

abstract：:An enzymatic profile of 20 strains of Pseudomonas maltophilia was undertaken with conventional plate tests, API ZYM, and 4-methylumbelliferyl-conjugated substrates. All strains produced DNase, RNase, arbutinase, esterases and lipases, mucinase, acid and alkaline phosphatases, alkaline pyrophosphate diesterase, phospho...

journal_title：Journal of clinical microbiology

authors： O'Brien M,Davis GH

更新日期：1982-09-01 00:00:00

abstract：:HIV-testing algorithms for preexposure prophylaxis (PrEP) should be optimized to minimize the risk of drug resistance, the time off PrEP required to evaluate false-positive screening results, and costs and to expedite the start of therapy for those confirmed to be infected. HIV rapid tests (RTs) for anti-HIV antibodie...

journal_title：Journal of clinical microbiology

authors： Guanira JV,Leigler T,Kallas E,Schechter M,Sharma U,Glidden D,Grant RM,iPrEx Study Team.

更新日期：2015-01-01 00:00:00

abstract：:Bartonella henselae or Bartonella elizabethae DNA from EDTA-anticoagulated blood samples obtained from four dogs was amplified and sequenced. The results showed that B. elizabethae should be added to the list of Bartonella species (i.e., B. vinsonii subsp. berkhoffii, B. henselae, and B. clarridgeiae) that are current...

journal_title：Journal of clinical microbiology

authors： Mexas AM,Hancock SI,Breitschwerdt EB

更新日期：2002-12-01 00:00:00

abstract：:Enterococcus cecorum (formerly Streptococcus cecorum), originally isolated from poultry intestines, has rarely been encountered in human diseases. A 60-year-old man with liver cirrhosis and hepatocellular carcinoma developed peritonitis on the seventh day of his hospitalization. Cultures of one blood sample and one as...

journal_title：Journal of clinical microbiology

authors： Hsueh PR,Teng LJ,Chen YC,Yang PC,Ho SW,Luh KT

更新日期：2000-06-01 00:00:00

abstract：:Since the introduction of antiviral compounds such as lamivudine and famciclovir in the treatment schedules of patients with chronic hepatitis B virus (HBV) infection, the accumulation of a variety of mutations in the HBV polymerase gene has been observed. The selection of these mutations is generally considered the c...

journal_title：Journal of clinical microbiology

authors： Stuyver L,Van Geyt C,De Gendt S,Van Reybroeck G,Zoulim F,Leroux-Roels G,Rossau R

更新日期：2000-02-01 00:00:00

abstract：:It has been shown that some, but not all, Escherichia coli strains isolated from urine adhere, in vitro, to the surface of uroepithelial or vaginal cells. In the present study, 212 strains, isolated from urine of 212 infected patients, were tested for adhesion by using an in vitro human cell line assay. A variable deg...

journal_title：Journal of clinical microbiology

authors： Chabanon G,Hartley CL,Richmond MH

更新日期：1979-10-01 00:00:00

abstract：:An automated cultivation system for mycobacteria, the MGIT 960 system (MGIT system), was compared in the clinical routine with two variants of Löwenstein-Jensen (L-J) medium. A total of 152 isolates were recovered from 2,015 specimens: 139 (91%) with the MGIT system and 127 (84%) with L-J media (P = 0.05). These inclu...

journal_title：Journal of clinical microbiology

authors： Katila ML,Katila P,Erkinjuntti-Pekkanen R

更新日期：2000-03-01 00:00:00

abstract：:We describe a new method which uses cold absolute methanol-prefixed cells for adherence of enteropathogenic Escherichia coli to HEp-2 cells. We found that a method using bacteria grown in Penassay broth to 10(6) to 10(7) CFU/ml and incubated with prefixed cells for 3 h at 37 degrees C, showed 100% sensitivity and spec...

journal_title：Journal of clinical microbiology

authors： Zepeda-Lopez HM,Gonzalez-Lugo GM

更新日期：1995-05-01 00:00:00

abstract：:Conventional culture techniques are limited in the ability to detect multiple Streptococcus pneumoniae serotypes in nasopharyngeal (NP) secretions. We developed an immunoblot (IB) method with monoclonal antibodies (MAbs) to detect S. pneumoniae and to identify serotypes. NP specimens stored in skim milk-tryptone-gluco...

journal_title：Journal of clinical microbiology

authors： Bronsdon MA,O'Brien KL,Facklam RR,Whitney CG,Schwartz B,Carlone GM

更新日期：2004-04-01 00:00:00

abstract：:PCR was used to amplify a 537-bp region of an Ehrlichia ewingii gene encoding a homologue of the 28-kDa major antigenic protein (P28) of Ehrlichia chaffeensis. The E. ewingii p28 gene homologue was amplified from DNA extracted from whole blood obtained from four humans and one canine with confirmed cases of infection....

journal_title：Journal of clinical microbiology

authors： Gusa AA,Buller RS,Storch GA,Huycke MM,Machado LJ,Slater LN,Stockham SL,Massung RF

更新日期：2001-11-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay for the detection of Shiga toxins (Premier EHEC assay; Meridian Diagnostics, Inc.) was compared to conventional sorbitol-MacConkey culture for the recovery of enterohemorrhagic Escherichia coli. A total of 74 enteric pathogens, including 8 E. coli O157:H7 isolates, were recovered f...

journal_title：Journal of clinical microbiology

authors： Kehl KS,Havens P,Behnke CE,Acheson DW

更新日期：1997-08-01 00:00:00

abstract：:Forty-eight clinical Acinetobacter isolates with different epidemic behavior were investigated for the presence of integrons and plasmids and for antibiotic susceptibility. Integrons were demonstrated in 50% of the strains by an integrase gene PCR. Epidemic strains of Acinetobacter baumannii were found to contain sign...

journal_title：Journal of clinical microbiology

authors： Koeleman JG,Stoof J,Van Der Bijl MW,Vandenbroucke-Grauls CM,Savelkoul PH

更新日期：2001-01-01 00:00:00

abstract：:Two national surveys were conducted to describe the incidence and prevalence of Enterobacter aerogenes in 21 Belgian hospitals in 1996 and 1997 and to characterize the genotypic diversity and the antimicrobial resistance profiles of clinical strains of E. aerogenes isolated from hospitalized patients in Belgium in 199...

journal_title：Journal of clinical microbiology

authors： De Gheldre Y,Struelens MJ,Glupczynski Y,De Mol P,Maes N,Nonhoff C,Chetoui H,Sion C,Ronveaux O,Vaneechoutte M,Groupement pour le Dépistage, l'Etude et la Prevention des Infections Hospitalières (GDEPIH-GOSPIZ).

更新日期：2001-03-01 00:00:00

abstract：:A sensitive detection system specific for Legionella pneumophila in water was developed. This system is based on amplification of a chromosomal DNA sequence from L. pneumophila by the polymerase chain reaction, followed by detection of the amplified product by hybridization of a radiolabeled oligodeoxynucleotide. Afte...

journal_title：Journal of clinical microbiology

authors： Starnbach MN,Falkow S,Tompkins LS

更新日期：1989-06-01 00:00:00

abstract：:Flagellar (H) antigens are mostly encoded by genes at the fliC locus in E. coli. We have sequenced 11 H7 fliC genes from Escherichia coli strains that belong to seven O serotypes. These sequences, together with those of nine other H7 fliC genes (from strains of three different O serotypes) sequenced recently (S. D. Re...

journal_title：Journal of clinical microbiology

authors： Wang L,Rothemund D,Curd H,Reeves PR

更新日期：2000-05-01 00:00:00

abstract：:Since January 1996, and over a 3-year time span, a significant spread of serotype 6B multidrug-resistant (MDR) pneumococci, susceptible to penicillin and resistant to erythromycin, clindamycin, tetracycline, chloramphenicol, and trimethoprim-sulfamethoxazole, was noted in young carriers living in central and southern ...

journal_title：Journal of clinical microbiology

authors： Syrogiannopoulos GA,Bogaert D,Grivea IN,Beratis NG,De Groot R R,Hermans PW

更新日期：2001-02-01 00:00:00

abstract：:Lower levels of performance of human papillomavirus (HPV) typing assays in studies using formalin-fixed paraffin-embedded (FFPE) tissue compared to those using exfoliated cervical cells have been reported. The interpretation of current studies is limited by bias in inclusion criteria, sample matching, and methods of c...

journal_title：Journal of clinical microbiology

authors： Dal Bello B,Spinillo A,Alberizzi P,Cesari S,Gardella B,Silini EM

更新日期：2009-07-01 00:00:00

abstract：:A simple agar-well diffusion bioassay suitable for measurement of flucytosine or ketoconazole was developed by using Candida pseudotropicalis ATCC 46764 as the assay organism. A test medium composed of (per liter) 7 g of Trypticase peptone, 7 g of YNB (yeast-nitrogen base), 15 g of glucose, and 15 g of agar was seeded...

journal_title：Journal of clinical microbiology

authors： Bodet CA 3rd,Jorgensen JH,Drutz DJ

更新日期：1985-08-01 00:00:00

abstract：:Antibodies to human papilloma virus (HPV) type 16 (HPV-16) E6 and E7 proteins in serum are markers for HPV-associated invasive cervical carcinoma. We compared two assays, a radioimmunoprecipitation assay with in vitro-translated HPV-16 E6 and E7 proteins and an enzyme-linked immunosorbent assay (ELISA) with E6 and E7 ...

journal_title：Journal of clinical microbiology

authors： Sun Y,Shah KV,Müller M,Muñoz N,Bosch XF,Viscidi RP

更新日期：1994-09-01 00:00:00

abstract：:We have recently developed a PCR assay for detection of Mycobacterium spp. at the genus level based on the Cobas Amplicor platform. The sensitivities for smear-positive and smear-negative specimens were found to be 100% and 47.9%, respectively. The specificity was 97.7%, the positive predictive value 84.6%, and the ne...

journal_title：Journal of clinical microbiology

authors： Peter-Getzlaff S,Lüthy J,Voit A,Bloemberg GV,Böttger EC

更新日期：2010-11-01 00:00:00