当前位置： SCI文献检索 > JOURNAL OF CLINICAL MICROBIOLOGY期刊下所有文献 > Assessment of fluorescent in situ hybridization and PCR-based methods for rapid identification of Burkholderia cepacia complex organisms directly from sputum samples.

Assessment of fluorescent in situ hybridization and PCR-based methods for rapid identification of Burkholderia cepacia complex organisms directly from sputum samples.

Abstract：

:Several species within the Burkholderia cepacia complex (BCC) have emerged as significant opportunistic pathogens of patients with cystic fibrosis (CF). BCC infection is typically associated with a poor clinical prognosis and decreased survival. These factors, combined with the existence of highly transmissible epidemic strains, have resulted in strict segregation of BCC- and non-BCC-infected patients to minimize cross infection. Accurate and rapid diagnosis of infections is essential to enable appropriate patient management. However, the rapidly evolving taxonomy of BCC poses a considerable challenge to diagnostics. In the present study, we assessed a commercially available fluorescent in situ hybridization (FISH) assay (seaFAST Cystic Fibrosis I kit) and a novel rRNA gene-based PCR assay for the rapid identification of BCC-positive sputa, irrespective of the BCC species. We report that, while the FISH assay fails to identify all BCC species, it does identify the majority of species, including the two most clinically relevant species, B. multivorans and B. cenocepacia. The sensitivity of the assay applied to sputum was limited by nonspecific background fluorescence. While sputum processing was optimized to minimize background, the resulting sensitivity for BCC detection was 8 x 10(5) CFU/ml. In contrast, the novel PCR assay reported herein exhibits 100% sensitivity and specificity for all BCC species and can detect 10(4) CFU/ml when applied to sputum. This novel rRNA gene-based assay is currently the most sensitive BCC-specific PCR assay for the detection of BCC direct from clinical samples and as such is a valuable addition to the field of BCC diagnostics.

journal_name

J Clin Microbiol

journal_title

Journal of clinical microbiology

authors

Brown AR,Govan JR

doi

10.1128/JCM.00147-07

subject

Has Abstract

pub_date

2007-06-01 00:00:00

pages

1920-6

issue

6

eissn

0095-1137

issn

1098-660X

pii

JCM.00147-07

journal_volume

45

pub_type

杂志文章

相关文献

JOURNAL OF CLINICAL MICROBIOLOGY文献大全
  • Characterization of Shiga toxin-producing Escherichia coli strains isolated from human patients in Germany over a 3-year period.

    abstract：:We have investigated 677 Shiga toxin-producing Escherichia coli (STEC) strains from humans to determine their serotypes, virulence genes, and clinical signs in patients. Six different Shiga toxin types (1, 1c, 2, 2c, 2d, and 2e) were distributed in the STEC strains. Intimin (eae) genes were present in 62.6% of the str...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章,多中心研究

    doi：10.1128/jcm.42.3.1099-1108.2004

    authors： Beutin L,Krause G,Zimmermann S,Kaulfuss S,Gleier K

    更新日期：2004-03-01 00:00:00

  • Bluetongue virus: comparative evaluation of enzyme-linked immunosorbent assay, immunodiffusion, and serum neutralization for detection of viral antibodies.

    abstract：:Comparative studies on the detection of bovine serum immunoglobulin G antibodies to bluetongue virus with an enzyme-linked immunosorbent assay, an immunodiffusion method, and a serum neutralization assay demonstrated complete concordance between the enzyme-linked immunosorbent assay and the serum neutralization assay ...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.15.1.159-162.1982

    authors： Poli G,Stott J,Liu YS,Manning JS

    更新日期：1982-01-01 00:00:00

  • Diagnosis of streptococcal pharyngitis by detection of Streptococcus pyogenes in posterior pharyngeal versus oral cavity specimens.

    abstract：:Carbohydrate antigen detection, nucleic acid probe detection, and bacterial culture are commonly used to confirm group A streptococcus (GAS) pharyngitis. Compared to standard throat swab specimens, the sensitivities of these tests with mouth specimens are poor. When testing for GAS pharyngitis, the throat remains the ...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.00797-06

    authors： Fox JW,Marcon MJ,Bonsu BK

    更新日期：2006-07-01 00:00:00

  • Detection of human cytomegalovirus in clinical specimens by centrifugation culture with a nonhuman cell line.

    abstract：:The sensitivities of MRC-5 and mink lung (ML) cells in centrifugation culture were compared simultaneously for the detection of cytomegalovirus (CMV) IE antigen (immediate-early antigen) from clinical specimens. Of 413 samples assayed, 51 (12%) were positive for CMV by both centrifugation and standard cell culture. At...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.30.4.1045-1048.1992

    authors： Gleaves CA,Hursh DA,Meyers JD

    更新日期：1992-04-01 00:00:00

  • In vitro amphotericin B resistance in clinical isolates of Aspergillus terreus, with a head-to-head comparison to voriconazole.

    abstract：:Amphotericin B therapy continues to be the "gold standard" in the treatment of invasive aspergillosis in the immunocompromised host. Although Aspergillus fumigatus and Aspergillus flavus constitute the major species, several reports have described invasive pulmonary or disseminated disease due to the less common Asper...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.37.7.2343-2345.1999

    authors： Sutton DA,Sanche SE,Revankar SG,Fothergill AW,Rinaldi MG

    更新日期：1999-07-01 00:00:00

  • Rapid screening for B19 parvovirus DNA in clinical specimens with a digoxigenin-labeled DNA hybridization probe.

    abstract：:A rapid dot blot hybridization assay for the detection of B19 parvovirus DNA in human sera was developed. Small portions of four serum samples were mixed, filtered onto a nylon membrane, and hybridized with a digoxigenin-labeled DNA probe; for each membrane, 380 serum samples could be tested. When a dot was positive b...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.28.11.2496-2499.1990

    authors： Zerbini M,Musiani M,Venturoli S,Gallinella G,Gibellini D,Gentilomi G,La Placa M

    更新日期：1990-11-01 00:00:00

  • First report of subcutaneous phaeohyphomycosis of the foot caused by Phoma minutella.

    abstract：:Phoma minutella, a dematiaceous hyphomycete, was isolated to the exclusion of all other fungi from a subcutaneous inflammatory process on the foot of a farmer undergoing corticosteroid therapy for myasthenia gravis. Isolation was achieved on several nutrient media. Examination of stained smears and sections revealed d...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.25.12.2395-2397.1987

    authors： Baker JG,Salkin IF,Forgacs P,Haines JH,Kemna ME

    更新日期：1987-12-01 00:00:00

  • Evaluation of E-Test for determination of antimicrobial MICs for Pseudomonas aeruginosa isolates from cystic fibrosis patients.

    abstract：:We determined the E-Test and National Committee for Clinical Laboratory Standards standardized agar dilution MICs of ceftazidime, ciprofloxacin, piperacillin, and tobramycin for Pseudomonas aeruginosa during tests of 100 rough and mucoid P. aeruginosa isolates from cystic fibrosis patients. The levels of agreement (+/...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.33.12.3191-3193.1995

    authors： Marley EF,Mohla C,Campos JM

    更新日期：1995-12-01 00:00:00

  • Survival of anaerobic and aerobic bacteria on cotton swabs in three transport systems.

    abstract：:The capacity of aerobic and anaerobic bacteria to survive on cotton swabs placed into a dry gassed-out CO2-filled tube (DGT), dry sterile aerobic tube (DAT), and a tube containing a modified Stuarts' transport medium (MST), was assessed. Pseudomonas aeruginosa increased in numbers by 2 and 3 logs when stored in MST a...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.1.2.196-200.1975

    authors： Yrios JW,Balish E,Helstad A,Field C,Inhorn S

    更新日期：1975-02-01 00:00:00

  • Detection of immune-complex-dissociated nonstructural-1 antigen in patients with acute dengue virus infections.

    abstract：:Accurate and timely diagnosis of dengue virus (DEN) infections is essential for the differential diagnosis of patients with febrile illness and hemorrhagic fever. In the present study, the diagnostic value of a newly developed immune-complex dissociated nonstructural-1 (NS-1) antigen dot blot immunoassay (DBI) was com...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/jcm.41.9.4154-4159.2003

    authors： Koraka P,Burghoorn-Maas CP,Falconar A,Setiati TE,Djamiatun K,Groen J,Osterhaus AD

    更新日期：2003-09-01 00:00:00

  • Clinical implications of evolutionary patterns of homologous, full-length hepatitis B virus quasispecies in different hosts after perinatal infection.

    abstract：:Chronic hepatitis B virus (HBV) infection via perinatal transmission is common in the Asia-Pacific region, but related quasispecies (QS) characteristics are not yet defined. To investigate the homologous, full-length HBV QS after perinatal infection and their clinical implications, five pairs of mother-daughter patien...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.03338-13

    authors： Liu F,Yu DM,Huang SY,Yu JL,Zhang DH,Gong QM,Zhang XX

    更新日期：2014-05-01 00:00:00

  • Clinical significance of Propionibacterium acnes recovered from blood cultures: analysis of 524 episodes.

    abstract：:Of 522 patients with Propionibacterium acnes bacteremia (PAB), 18 (3.5%) had clinically significant PAB. Of these 18 patients, 10 (55.6%) had hospital-acquired bacteremia and 6 (33.3%) had undergone invasive procedures before development of PAB. One patient with a ventricular septal defect presented with infective end...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.01842-10

    authors： Park HJ,Na S,Park SY,Moon SM,Cho OH,Park KH,Chong YP,Kim SH,Lee SO,Kim YS,Woo JH,Kim MN,Choi SH

    更新日期：2011-04-01 00:00:00

  • Visible DNA Microarray System as an Adjunctive Molecular Test in Identification of Pathogenic Fungi Directly from a Blood Culture Bottle.

    abstract：:A DNA microarray platform, based on the nucleotide sequences of the internal transcribed spacer regions (ITS1 and ITS2) of the rRNA gene, was developed to identify 32 fungal pathogens at the species level. The probe sequences were spotted onto polycarbonate slides with a mini-microarray printer, and after the hybridiz...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.01908-17

    authors： Sturaro LL,Gonoi T,Busso-Lopes AF,Tararam CA,Levy CE,Lyra L,Trabasso P,Schreiber AZ,Kamei K,Moretti ML

    更新日期：2018-04-25 00:00:00

  • Further modifications of the auxanographic method for identification of yeasts.

    abstract：:A modified auxanographic carbohydrate assimilation procedure for the identification of medically important yeasts is described. This method employs a heavy inoculum of unstarved yeasts, autoclaved yeast assimilation medium, pour plates of shallow depth, and commercially available carbohydrate-impregnated disks. The ac...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：

    authors： Mickelsen PA,McCarthy LR,Propst MA

    更新日期：1977-03-01 00:00:00

  • Evaluation of the Vidas Chlamydia test to detect and verify Chlamydia trachomatis in urogenital specimens.

    abstract：:The Vidas Chlamydia test (CHL) is an automated enzyme-linked immunofluorescence assay for the detection of Chlamydia trachomatis. Positive and equivocal results are confirmed with a blocking assay. A mouse monoclonal antibody directed against the chlamydial lipopolysaccharides was used for the test. The CHL assay is w...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章,多中心研究

    doi：10.1128/JCM.35.8.2102-2106.1997

    authors： Schachter J,Jones RB,Butler RC,Rice B,Brooks D,Van der Pol B,Gray M,Moncada J

    更新日期：1997-08-01 00:00:00

  • Identification of Staphylococcus species of bovine origin with the API Staph-Ident system.

    abstract：:The API Staph-Ident system was evaluated as a means for identifying the species of bovine strains of staphylococci routinely isolated from quarter-milk samples. The species identity of 314 of 581 (54%) isolates of staphylococci was correctly determined by this method. The API Staph-Ident system was more accurate in co...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.18.5.1212-1219.1983

    authors： Langlois BE,Harmon RJ,Akers K

    更新日期：1983-11-01 00:00:00

  • Novel IS711 chromosomal location useful for identification of marine mammal Brucella genotype ST27, which is associated with zoonotic infection.

    abstract：:We report a novel IS711 chromosomal location that is specific for the Brucella genotype ST27 previously associated with Pacific marine mammals and human zoonotic infection in New Zealand and Peru. Our data support the previous observation that this peculiar genotype is distinct from those commonly isolated from the At...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.05238-11

    authors： Cloeckaert A,Bernardet N,Koylass MS,Whatmore AM,Zygmunt MS

    更新日期：2011-11-01 00:00:00

  • Enzyme-linked immunosorbent assay for serological diagnosis of Chagas' disease employing a Trypanosoma cruzi recombinant antigen that consists of four different peptides.

    abstract：:Serological tests to detect Trypanosoma cruzi antibodies have been used for screening blood donors, for epidemic studies, and for diagnosis of probably infected persons. Among different tests, the enzyme-linked immunosorbent assay (ELISA) with total, semipurified, or synthetic antigens has been widely used, mainly due...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.39.12.4390-4395.2001

    authors： Ferreira AW,Belem ZR,Lemos EA,Reed SG,Campos-Neto A

    更新日期：2001-12-01 00:00:00

  • Point-Counterpoint: Meningitis/Encephalitis Syndromic Testing in the Clinical Laboratory.

    abstract：:INTRODUCTIONSyndromic panels were first FDA cleared for detection of respiratory pathogens in 2008. Since then, other panels have been approved by the FDA, and most recently, the FilmArray meningitis/encephalitis panel (BioFire, Salt Lake City, UT) has become available. This assay detects 14 targets within 1 h and inc...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.00018-18

    authors： Dien Bard J,Alby K

    更新日期：2018-03-26 00:00:00

  • Buruli ulcer disease in travelers and differentiation of Mycobacterium ulcerans strains from northern Australia.

    abstract：:Buruli ulcer (BU) is a necrotizing infection of skin and soft tissue caused by Mycobacterium ulcerans. In Australia, most cases of BU are linked to temperate, coastal Victoria and tropical, northern Queensland, and strains from these regions are distinguishable by variable-number tandem repeat (VNTR) typing. We presen...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.01324-12

    authors： Lavender CJ,Globan M,Johnson PD,Charles PG,Jenkin GA,Ghosh N,Clark BM,Martinello M,Fyfe JA

    更新日期：2012-11-01 00:00:00

  • Men and Women Have Similar Neisseria gonorrhoeae Bacterial Loads: a Comparison of Three Anatomical Sites.

    abstract：:Neisseria gonorrhoeae is a common bacterial sexually transmitted infection (STI). Currently, there are limited data on the bacterial load in both men and women and on both genital and extragenital sites. Therefore, we quantified N. gonorrhoeae bacterial loads in a large population of women, heterosexual men, and men w...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.01171-20

    authors： van der Veer BMJW,Hoebe CJPA,Dukers-Muijrers NHTM,van Alphen LB,Wolffs PFG

    更新日期：2020-10-21 00:00:00

  • Comparison of BD GeneOhm Cdiff and Seegene Seeplex ACE PCR assays using toxigenic Clostridium difficile culture for direct detection of tcdB from stool specimens.

    abstract：:We evaluated the performances of 2 PCR assays (BD GeneOhm and Seegene ACE) for direct detection of tcdB from stool specimens. The concordance rate between BD and Seegene was 96.3%. The sensitivities, specificities, positive predictive values (PPVs), and negative predictive values (NPVs) of BD and Seegene were 95.7%, 9...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.01440-12

    authors： Shin BM,Mun SJ,Yoo SJ,Kuak EY

    更新日期：2012-11-01 00:00:00

  • Characterization and description of "Campylobacter upsaliensis" isolated from human feces.

    abstract：:During a 3-year period, "Campylobacter upsaliensis" was isolated from 99 patients. Phenotypic characterization and numerical analysis of protein electrophoregrams showed evidence that "C. upsaliensis" is a distinct Campylobacter species with unique characteristics. The MBCs of 13 antibiotics were determined. In genera...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.28.5.1039-1046.1990

    authors： Goossens H,Pot B,Vlaes L,Van den Borre C,Van den Abbeele R,Van Naelten C,Levy J,Cogniau H,Marbehant P,Verhoef J

    更新日期：1990-05-01 00:00:00

  • Differentiation of Campylobacter coli, Campylobacter jejuni, Campylobacter lari, and Campylobacter upsaliensis by a multiplex PCR developed from the nucleotide sequence of the lipid A gene lpxA.

    abstract：:We describe a multiplex PCR assay to identify and discriminate between isolates of Campylobacter coli, Campylobacter jejuni, Campylobacter lari, and Campylobacter upsaliensis. The C. jejuni isolate F38011 lpxA gene, encoding a UDP-N-acetylglucosamine acyltransferase, was identified by sequence analysis of an expressio...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.42.12.5549-5557.2004

    authors： Klena JD,Parker CT,Knibb K,Ibbitt JC,Devane PM,Horn ST,Miller WG,Konkel ME

    更新日期：2004-12-01 00:00:00

  • GeneScan reverse transcription-PCR assay for detection of six common respiratory viruses in young children hospitalized with acute respiratory illness.

    abstract：:A reverse transcription-PCR (RT-PCR) assay based on automated fluorescent capillary electrophoresis and GeneScan software analysis was developed to detect six common respiratory viruses in clinical specimens from young children. Assays for human respiratory syncytial virus (HRSV); human parainfluenza viruses 1, 2, and...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/jcm.41.9.4298-4303.2003

    authors： Erdman DD,Weinberg GA,Edwards KM,Walker FJ,Anderson BC,Winter J,González M,Anderson LJ

    更新日期：2003-09-01 00:00:00

  • Direct antimicrobial susceptibility testing for acute urinary tract infections in women.

    abstract：:Despite its theoretical advantages, direct antimicrobial susceptibility testing (DST) of urine specimens remains controversial largely because of concerns regarding its accuracy, particularly with mixed cultures. To evaluate the performance of DST in the setting of acute urinary tract infection (UTI), we performed DST...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.33.9.2316-2323.1995

    authors： Johnson JR,Tiu FS,Stamm WE

    更新日期：1995-09-01 00:00:00

  • Rapid detection of methicillin-resistant staphylococci from blood culture bottles by using a multiplex PCR assay.

    abstract：:Rapid detection and accurate identification of methicillin-resistant staphylococci are critical for the effective management of infections caused by these organisms. We describe a multiplex PCR-based assay for the direct detection of methicillin-resistant staphylococci from blood culture bottles (BacT/Alert; Organon-T...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/jcm.40.8.2786-2790.2002

    authors： Louie L,Goodfellow J,Mathieu P,Glatt A,Louie M,Simor AE

    更新日期：2002-08-01 00:00:00

  • Comparison of an enzyme-linked immunosorbent assay with indirect hemagglutination and hemagglutination inhibition for determination of rubella virus antibody: evaluation of immune status with commercial reagents in a clinical laboratory.

    abstract：:Comparative evaluations of immune status for rubella virus are described for enzyme-linked immunosorbent assay, hemagglutination inhibition, and indirect hemagglutination. A 92.1% agreement between enzyme-linked immunosorbent assay and indirect hemagglutination assay was demonstrated for rubella immune status. Enzyme-...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.17.1.106-108.1983

    authors： Truant AL,Barksdale BL,Huber TW,Elliott LB

    更新日期：1983-01-01 00:00:00

  • Evidence for two serotype G3 subtypes among equine rotaviruses.

    abstract：:Ten cultivable equine rotavirus isolates, two of North American, six of British, and two of Irish origin, were compared with standard rotavirus strains and with each other by cross neutralization, neutralization with a panel of monoclonal antibodies (MAbs), hybridization to a simian rotavirus (SA-11) VP7 gene probe, a...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.30.2.485-491.1992

    authors： Browning GF,Chalmers RM,Fitzgerald TA,Snodgrass DR

    更新日期：1992-02-01 00:00:00

  • Comparative evaluation of three different commercial blood culture media for recovery of anaerobic organisms.

    abstract：:A comparison of three different commercial media was made to assess their recovery of anaerobic organisms from the blood stream. The three media used were the 50-ml brain heart infusion broth with added CO2 (Pfizer), the 50-ml Thiol broth with added CO2 (Difco), and the 50-ml prereduced, supplemented peptone broth in ...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：

    authors： Mangels JI,Lindberg LH,Vosti KL

    更新日期：1977-05-01 00:00:00