Direct antimicrobial susceptibility testing for acute urinary tract infections in women.

abstract：:An alpha-toxin disk test is described in which group B streptococci completed the hemolysis of sheep erythrocytes partially lysed by the alpha-toxin of Clostridium perfringens. The test was performed satisfactorily on the sheep blood agar primary isolation plate, as well as on pure cultures. A total of 95% of strains ...

journal_title：Journal of clinical microbiology

authors： Smith JA,Ngui-Yen JH

更新日期：1980-07-01 00:00:00

abstract：:A direct and an indirect enzyme immunoassay (EIA) were evaluated against a standard of electron microscopy after direct ultracentrifugation of the specimen for their performances in detecting rotaviruses. The indirect EIA had variable background activity which influenced test specificity. The indirect EIA control (tes...

journal_title：Journal of clinical microbiology

authors： Hammond GW,Ahluwalia GS,Barker FG,Horsman G,Hazelton PR

更新日期：1982-07-01 00:00:00

abstract：:A diagnostic test for feline infectious peritonitis virus (FIPV) infection based on a nested PCR (nPCR) assay was developed and tested with FIPV, feline enteric coronavirus (FECV), canine coronavirus (CCV), and transmissible gastroenteritis virus (TGEV) and clinical fluid samples from cats with effusive feline infecti...

journal_title：Journal of clinical microbiology

authors： Gamble DA,Lobbiani A,Gramegna M,Moore LE,Colucci G

更新日期：1997-03-01 00:00:00

abstract：:An enzyme-linked immunosorbent microassay using nitrocellulose paper as the solid-phase support was developed for the detection of peste-des-petits-ruminants virus antigens in infected caprine tissue homogenates. Dots of tissue homogenates were applied to nitrocellulose papers, and any unreacted sites were blocked wit...

journal_title：Journal of clinical microbiology

authors： Obi TU,Ojeh CK

更新日期：1989-09-01 00:00:00

abstract：:Detection of Staphylococcus aureus isolates with intermediate vancomycin susceptibility (VISA) and heteroresistance (hVISA) remains problematic. The population analysis profile/area under the curve (PAP/AUC) is the gold standard but is cumbersome. We compared the performance of two Etest screening methods (macromethod...

journal_title：Journal of clinical microbiology

authors： Riederer K,Shemes S,Chase P,Musta A,Mar A,Khatib R

更新日期：2011-06-01 00:00:00

abstract：:An isolate of Proteus mirabilis recovered from blood cultures of a diabetic patient was shown to be resistant to imipenem, meropenem, and ertapenem by disk diffusion susceptibility testing. Amplification of whole-cell and/or plasmid DNA recovered from the isolate with primers specific for the bla(KPC) carbapenemase ge...

journal_title：Journal of clinical microbiology

authors： Tibbetts R,Frye JG,Marschall J,Warren D,Dunne W

更新日期：2008-09-01 00:00:00

abstract：:The API 20S system (Analytab Products, Plainview, N.Y.) and the AutoMicrobic Gram-Positive Identification system (GPI; Vitek Systems, Hazelwood, Mo.) were evaluated for their capacity to identify the non-beta-hemolytic streptococci and aerococci to the species level. The 20S system identified 86% (six of seven strains...

journal_title：Journal of clinical microbiology

authors： Facklam R,Bosley GS,Rhoden D,Franklin AR,Weaver N,Schulman R

更新日期：1985-04-01 00:00:00

abstract：:Immune pressure on lentiviruses exerted by cytotoxic T lymphocytes (CTL) selects for virus CTL epitope mutations. Currently employed methods for monitoring emerging CTL epitope mutations rely on the labor-intensive and time-consuming techniques of virus population or clonal sequencing. Here we describe the development...

journal_title：Journal of clinical microbiology

authors： Peyerl FW,Barouch DH,Bazick HS,Manuel E,Letvin NL

更新日期：2005-09-01 00:00:00

abstract：:A variety of bacterins, vaccines, and antisera retained greater than 90% of their original level of mercurial preservative after lyophilization, and this might influence certain uses of these products. ...

journal_title：Journal of clinical microbiology

authors： Pemberton JR

更新日期：1975-12-01 00:00:00

abstract：:The nucleic acid sequence of the putative 5'-untranslated (5PUT) region of hepatitis C virus (HCV), determined for samples obtained from a variety of geographic origins, was found to be over 98% conserved among all isolates. On the basis of this signature sequence for HCV, a viral RNA assay was developed by using cDNA...

journal_title：Journal of clinical microbiology

authors： Cha TA,Kolberg J,Irvine B,Stempien M,Beall E,Yano M,Choo QL,Houghton M,Kuo G,Han JH

更新日期：1991-11-01 00:00:00

abstract：:In a recent genotypic survey of beta-lactam-resistant pneumococci recovered in different areas of United States during 1997, eight clonal types that each represented 3 to 40 isolates accounted for 134 of 144 isolates (G. Gherardi, C. Whitney, R. Facklam, and B. Beall, J. Infect. Dis. 181:216-229, 2000). We determined ...

journal_title：Journal of clinical microbiology

authors： Beall B,Gherardi G,Facklam RR,Hollingshead SK

更新日期：2000-10-01 00:00:00

abstract：:For testing bacterial susceptibility to PD131628, a 5-micrograms disk and the following tentative interpretive criteria may be used: > or = 19 mm for susceptible (MIC, < or = 1.0 micrograms/ml), 16 to 18 mm for intermediate (MIC, 2.0 micrograms/ml), and < or = 15 mm for resistant (MIC, > or = 4.0 micrograms/ml). For s...

journal_title：Journal of clinical microbiology

authors： Barry AL,Fuchs PC,Allen SD,Tenover FC,Jorgensen JH,Reller LB

更新日期：1995-01-01 00:00:00

abstract：:Brevibacterium sp. was isolated from the blood of an acutely ill 18-year-old female with AIDS. The isolate was identified as Brevibacterium casei by use of carbohydrate assimilation tests. Treatment was successful with intravenously administered ciprofloxacin. To our knowledge, this is the first report of sepsis cause...

journal_title：Journal of clinical microbiology

authors： Brazzola P,Zbinden R,Rudin C,Schaad UB,Heininger U

更新日期：2000-09-01 00:00:00

abstract：:Indirect and double-antibody sandwich enzyme-linked immunosorbent assay techniques were compared for serotyping 42 herpes simplex virus isolates. Both procedures gave similar results, which were in complete agreement with those obtained by inhibition of the indirect hemagglutination test. The indirect technique proved...

journal_title：Journal of clinical microbiology

authors： Gerna G,Battaglia M,Revello MG,Gerna MT

更新日期：1983-05-01 00:00:00

abstract：:Commercial chemiluminescent DNA probes (Accuprobe; Gen-Probe, San Diego, Calif.) for the identification of Mycobacterium tuberculosis (MTB) complex, M. avium complex (MAC), M. gordonae, and M. kansasii were evaluated with 134 clinical isolates. These included 36 MTB complex, 40 MAC, 27 M. gordonae, 9 M. kansasii, and ...

journal_title：Journal of clinical microbiology

authors： Lebrun L,Espinasse F,Poveda JD,Vincent-Levy-Frebault V

更新日期：1992-09-01 00:00:00

abstract：:Candida dubliniensis is a newly recognized fungal pathogen causing mucosal disease in AIDS patients. Although preliminary studies indicate that most strains of C. dubliniensis are susceptible to established antifungal agents, fluconazole-resistant strains have been detected. Furthermore, fluconazole-resistant strains ...

journal_title：Journal of clinical microbiology

authors： Pfaller MA,Messer SA,Gee S,Joly S,Pujol C,Sullivan DJ,Coleman DC,Soll DR

更新日期：1999-03-01 00:00:00

abstract：:CDC eugonic oxidizer group 2 (EO-2) is a group of unclassified gram-negative bacterial strains isolated from various human sources. As determined by biochemical tests and analyses of fatty acid compositions, these organisms form a homogeneous group that appears to be distinct from but related to other Paracoccus speci...

journal_title：Journal of clinical microbiology

authors： Daneshvar MI,Hollis DG,Weyant RS,Steigerwalt AG,Whitney AM,Douglas MP,Macgregor JP,Jordan JG,Mayer LW,Rassouli SM,Barchet W,Munro C,Shuttleworth L,Bernard K

更新日期：2003-03-01 00:00:00

abstract：:The performance characteristics of three real-time influenza A/B virus reverse transcription-PCR (RT-PCR) assays and two real-time 2009 H1N1 RT-PCR assays were evaluated using previously characterized clinical specimens. A total of 150 respiratory specimens from children (30 influenza A/H1 virus-, 30 influenza A/H3 vi...

journal_title：Journal of clinical microbiology

authors： Selvaraju SB,Selvarangan R

更新日期：2010-11-01 00:00:00

abstract：:A slight modification of the chemically defined medium of McVeigh and Morton resulted in an excellent substratum for the cultivation of Paracoccidioides brasiliensis yeast phase. ...

journal_title：Journal of clinical microbiology

authors： Restrepo A,Jiménez BE

更新日期：1980-08-01 00:00:00

abstract：:A commercially available latex agglutination reagent, Directigen (Hynson, Westcott & Dunning, Div. Becton Dickinson & Co., Baltimore, Md.), in which a murine monoclonal antibody to group B streptococcal (GBS) antigen is the active component, was evaluated by using body fluid specimens from 94 sick infants. Antigen was...

journal_title：Journal of clinical microbiology

authors： Rench MA,Metzger TG,Baker CJ

更新日期：1984-11-01 00:00:00

abstract：:Anti-idiotypic antibodies (anti-Ids) were raised in a rabbit against a murine monoclonal antibody (MAb) neutralizing the yeast killer toxin produced by a strain of Pichia (Hansenula) anomala. In an immunodiffusion test, the anti-Ids produced in the rabbit recognized the antigen-binding site of the MAb used as the immu...

journal_title：Journal of clinical microbiology

authors： Polonelli L,Morace G

更新日期：1988-03-01 00:00:00

abstract：:Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry with a Bruker Daltonics microflex LT system was applied to 90 well-characterized catalase-negative, Gram-positive cocci not belonging to the streptococci or enterococci. Biotyper version 2.0.43.1 software was used singly or in com...

journal_title：Journal of clinical microbiology

authors： Christensen JJ,Dargis R,Hammer M,Justesen US,Nielsen XC,Kemp M,Danish MALDI-TOF MS Study Group.

更新日期：2012-05-01 00:00:00

abstract：:The stability of cervical specimens in SurePath preservative fluid for human papillomavirus (HPV) testing with Roche cobas 4800 was determined using a panel of 308 pooled specimens from a colposcopy referral population. The SurePath specimens appeared to be stable for up to 10 weeks at ambient temperature for HPV test...

journal_title：Journal of clinical microbiology

authors： Gilbert L,Oates E,Ratnam S

更新日期：2013-10-01 00:00:00

abstract：:Several problems remain before molecular biology-based techniques, such as PCR, are widely accepted for the detection of infectious agents. Among the most formidable of these problems are the inability of the tests to distinguish between viable and nonviable organisms. We approached this problem by using the fact that...

journal_title：Journal of clinical microbiology

authors： Jou NT,Yoshimori RB,Mason GR,Louie JS,Liebling MR

更新日期：1997-05-01 00:00:00

abstract：:Serratia ficaria was first described in 1979 as part of the fig tree ecosystem (P.A.D. Grimont, F. Grimont, and M. P. Starr, Curr. Microbiol. 2:277-282, 1979). Since then, it has been isolated from clinical specimens from a few human patients (C. Bollet, J. Freney, P. de Micco, F. Grimont, and P.A.D. Grimont, Méd. Mal...

journal_title：Journal of clinical microbiology

authors： Darbas H,Jean-Pierre H,Paillisson J

更新日期：1994-09-01 00:00:00

abstract：:Arbitrarily primed polymerase chain reaction (AP-PCR) was used to characterize Legionella pneumophila serogroup 1. Cells from a single colony could be subtyped by AP-PCR within a few hours. The discrimination between strains of L. pneumophila serogroup 1 by AP-PCR was equivalent to that by monoclonal antibody analysis...

journal_title：Journal of clinical microbiology

authors： Gomez-Lus P,Fields BS,Benson RF,Martin WT,O'Connor SP,Black CM

更新日期：1993-07-01 00:00:00

abstract：:A set of universal degenerate primers which amplified, by PCR, a 600-bp oligomer encoding a portion of the 60-kDa heat shock protein (HSP60) of both Staphylococcus aureus and Staphylococcus epidermidis were developed. However, when used as a DNA probe, the 600-bp PCR product generated from S. epidermidis failed to cro...

journal_title：Journal of clinical microbiology

authors： Goh SH,Potter S,Wood JO,Hemmingsen SM,Reynolds RP,Chow AW

更新日期：1996-04-01 00:00:00

abstract：:Mucosal and systemic immunologic recognition of cagA by Helicobacter pylori-infected individuals is associated with peptic ulcer disease; however, in the laboratory, expression of cagA is subject to artificial conditions which may not accurately reflect the conditions in host tissues. Gastric antral and body biopsy sp...

journal_title：Journal of clinical microbiology

authors： Peek RM Jr,Miller GG,Tham KT,Pérez-Pérez GI,Cover TL,Atherton JC,Dunn GD,Blaser MJ

更新日期：1995-01-01 00:00:00

abstract：:A strain of Escherichia coli isolated from a child with diarrhea was biochemically similar and antigenically identical (O and K antigens) to the standard strain of E. coli O group 29 and was positive in the Serény test for invasiveness, which suggests that it can cause a Shigella-like illness. ...

journal_title：Journal of clinical microbiology

authors： Toledo MR,Reis MH,Almeida RG,Trabulsi LR

更新日期：1979-02-01 00:00:00

abstract：:The detection and quantitation of human parainfluenza virus type 1 (HPIV-1) RNA in nasal wash specimens from 49 children with lower respiratory infections were performed by a reverse transcription-PCR-enzyme hybridization assay (RT-PCR-EHA). The HPIV-1 RT-PCR-EHA was then used to test 40 samples from asymptomatic chil...

journal_title：Journal of clinical microbiology

authors： Fan J,Henrickson KJ

更新日期：1996-08-01 00:00:00