Use of a signature nucleotide sequence of hepatitis C virus for detection of viral RNA in human serum and plasma.

abstract：:Typical and atypical Campylobacter strains were isolated from the colons of cattle and swine by techniques that enabled the selective pressures of antibiotics to be avoided. Some cephalothin-susceptible strains and a strain with an indeterminate hippurate reaction were classified as Campylobacter coli by DNA homology ...

journal_title：Journal of clinical microbiology

authors： Ng LK,Taylor DE,Stiles ME

更新日期：1988-03-01 00:00:00

abstract：:Salmonella enterica serovar Heidelberg is the second most frequently occurring serovar in Quebec and the third-most prevalent in Canada. Given that conventional pulsed-field gel electrophoresis (PFGE) subtyping for common Salmonella serovars, such as S. Heidelberg, yields identical subtypes for the majority of isolate...

journal_title：Journal of clinical microbiology

authors： Bekal S,Berry C,Reimer AR,Van Domselaar G,Beaudry G,Fournier E,Doualla-Bell F,Levac E,Gaulin C,Ramsay D,Huot C,Walker M,Sieffert C,Tremblay C

更新日期：2016-02-01 00:00:00

abstract：:PCR amplification technology for the detection of epizootic hemorrhagic disease virus (EHDV) ribonucleic acid in cell culture and clinical specimens was developed. With oligoribonucleotide primers selected from genome segment 10 of EHDV serotype 1 (EHDV-1), which codes for two nonstructural proteins (NS3 and NS3a), th...

journal_title：Journal of clinical microbiology

authors： Aradaib IE,Wilson WC,Schore CE,Mohammed ME,Yilma TD,Cullor JS,Osburn BI

更新日期：1998-09-01 00:00:00

abstract：:The performances of a commercially available qualitative plasma PCR assay (AMPLICOR CMV test; Roche Diagnostics) and the pp65 antigenemia assay (AG) were evaluated for the monitoring of cytomegalovirus (CMV) viremia in 43 allogeneic stem cell transplant recipients. In addition, the suitabilities of both assays for tri...

journal_title：Journal of clinical microbiology

authors： Solano C,Muñoz I,Gutiérrez A,Farga A,Prósper F,García-Conde J,Navarro D,Gimeno C

更新日期：2001-11-01 00:00:00

abstract：:Two brands of thiosulfate citrate bile salts sucrose agar and Monsur taurocholate tellurite gelatin (TTG) agar were compared with two newly developed media, sucrose tellurite teepol agar and Vibrio parahaemolyticus agar for isolation and identification of Vibrio cholerae. The thiosulfate citrate bile salts sucrose and...

journal_title：Journal of clinical microbiology

authors： Morris GK,Merson MH,Huq I,Kibrya AK,Black R

更新日期：1979-01-01 00:00:00

abstract：:Characteristics differentiating Escherichia coli strains that cause cystitis or pyelonephritis from fecal E. coli remain incompletely defined, particularly among adult women in the United States. Accordingly, phylogenetic group, O antigens, and virulence factors (VFs) were analyzed among 329 E. coli isolates from the ...

journal_title：Journal of clinical microbiology

authors： Johnson JR,Owens K,Gajewski A,Kuskowski MA

更新日期：2005-12-01 00:00:00

abstract：:Ten cases of typhoid fever occurred between 24 August and 1 September 1986 in the vicinity of Silver Spring, Md. Shrimp salad served in a fast-food restaurant was implicated as the source of infection. Stool cultures were obtained from 104 employees, and serum Vi antibodies were assayed in 97 of the employees. Salmone...

journal_title：Journal of clinical microbiology

authors： Lin FY,Becke JM,Groves C,Lim BP,Israel E,Becker EF,Helfrich RM,Swetter DS,Cramton T,Robbins JB

更新日期：1988-06-01 00:00:00

abstract：:G3 rotaviruses have been reported rarely in cattle, and none have been characterized. We report the first genomic characterization of a bovine G3 rotavirus, CP-1, which had been biologically characterized in vivo and shown to cause age-independent diarrhea. CP-1 was a G3 rotavirus as its VP7 had 92 to 96% deduced amin...

journal_title：Journal of clinical microbiology

authors： El-Attar L,Dhaliwal W,Iturriza-Gómara M,Bridger JC

更新日期：2002-03-01 00:00:00

abstract：:A genotyping study of 285 Hybrid Capture 2 low-risk probe cocktail-positive specimens showed cross-reactivity with several untargeted human papillomavirus genotypes. Cross-reactivity was often clinically beneficial due to the detection of untargeted low-risk genotypes. A total of 8.4% of positive results, usually weak...

journal_title：Journal of clinical microbiology

authors： Poljak M,Kocjan BJ,Kovanda A,Lunar MM,Lepej SZ,Planinic A,Seme K,Vince A

更新日期：2009-08-01 00:00:00

abstract：:An indirect enzyme-linked immunosorbent assay was used for the screening of horse sera from The Gambia for antibodies against African horse sickness virus (AHSV). The AHSV antigen used for coating was semipurified according to the method of Manning and Chen (Curr. Microbiol. 4:381, 1980); control mock-infected Vero ce...

journal_title：Journal of clinical microbiology

authors： Staeuber N,Fye B,Zinsstag J,McCullough KC

更新日期：1993-08-01 00:00:00

abstract：:Clostridium difficile is the bacterium most commonly surmised to cause antimicrobial- and hospital-associated diarrhea in developed countries worldwide, and such infections are thought to be increasing in frequency and severity. A laboratory-based study was carried out to characterize C. difficile strains isolated fro...

journal_title：Journal of clinical microbiology

authors： Martin H,Willey B,Low DE,Staempfli HR,McGeer A,Boerlin P,Mulvey M,Weese JS

更新日期：2008-09-01 00:00:00

abstract：:Used peritoneal dialysis fluid was collected from patients undergoing continuous ambulatory peritoneal dialysis, and its pH and composition were assessed after incubation in either air or air with 5% CO2. Precipitation of calcium, magnesium, phosphate, and proteins occurred in the dialysis fluid incubated in air at 37...

journal_title：Journal of clinical microbiology

authors： Wilcox MH,Smith DG,Evans JA,Denyer SP,Finch RG,Williams P

更新日期：1990-10-01 00:00:00

abstract：:A PCR test based on insertion sequence IS1081 was developed to detect Mycobacterium tuberculosis complex organisms in the peripheral blood. The method was applied to blood samples from immunocompetent individuals with localized pulmonary tuberculosis. Seven of 16 (43.75%) blood samples were found to be positive for th...

journal_title：Journal of clinical microbiology

authors： Ahmed N,Mohanty AK,Mukhopadhyay U,Batish VK,Grover S

更新日期：1998-10-01 00:00:00

abstract：:Many laboratories are unable to perform colistin susceptibility testing. Diffusion-based antimicrobial susceptibility testing methods are not recommended, and not all laboratories have the capacity to perform broth microdilution (BMD). Using a multistep tiered approach, we investigated whether the adapted use of the M...

journal_title：Journal of clinical microbiology

authors： Lutgring JD,Kim A,Campbell D,Karlsson M,Brown AC,Burd EM

更新日期：2019-04-26 00:00:00

abstract：:We describe a rare case of Francisella novicida bacteremia following a near-drowning event in seawater. We highlight the challenges associated with laboratory identification of F. novicida and differences in the epidemiology of F. novicida and Francisella tularensis infections. ...

journal_title：Journal of clinical microbiology

authors： Brett M,Doppalapudi A,Respicio-Kingry LB,Myers D,Husband B,Pollard K,Mead P,Petersen JM,Whitener CJ

更新日期：2012-08-01 00:00:00

abstract：:Sheep and human erythrocytes, partially processed by Staphylococcus aureus or Clostridium perfringens, were susceptible to lysis in the presence of Propionibacterium acnes. P. acnes liberated a lipase that was detected on Tween 80 agar and also on phospholipase C-precipitated egg yolk agar. Such a lipase might have co...

journal_title：Journal of clinical microbiology

authors： Choudhury TK

更新日期：1978-08-01 00:00:00

abstract：:The sensitivities and specificities of three immunoassays for the detection of herpes simplex virus type 2 (HSV-2)-specific immunoglobulin G antibodies in serum, including the one-strip rapid immunoblot assay (RIBA; Chiron Corporation) and two indirect enzyme immunosorbent assays (EIA; Gull Laboratories and Centocor),...

journal_title：Journal of clinical microbiology

authors： Groen J,Van Dijk G,Niesters HG,Van Der Meijden WI,Osterhaus AD

更新日期：1998-03-01 00:00:00

abstract：:The semen plasma virus load is measured to ensure the safety of sperm processing during medically assisted procreation (MAP) for couples with a human immunodeficiency virus type 1 (HIV-1)-infected man. A practical, automated protocol using the COBAS Ampliprep CAP/CTM kit in the COBAS TaqMan96 system was developed to m...

journal_title：Journal of clinical microbiology

authors： Pasquier C,Sauné K,Raymond S,Moinard N,Daudin M,Bujan L,Izopet J

更新日期：2009-09-01 00:00:00

abstract：:First-void urine (FVU) is the preferred specimen for the diagnosis of urogenital Chlamydia trachomatis infection in men. We have developed FirstBurst, a urine collection device that collects the first 4 to 5 ml of FVU and yields a specimen with a sixfold higher C. trachomatis organism load than the regular urine cup b...

journal_title：Journal of clinical microbiology

authors： Wisniewski CA,White JA,Michel CE,Mahilum-Tapay L,Magbanua JP,Nadala EC Jr,Barber PJ,Goh BT,Lee HH

更新日期：2008-04-01 00:00:00

abstract：:Blastomyces dermatitidis and Histoplasma capsulatum are dimorphic fungi that often cause self-limited respiratory infections. However, they may also cause severe disseminated disease, depending on the level of the exposure to the organism and the host immune status. In addition, patients with infections caused by thes...

journal_title：Journal of clinical microbiology

authors： Babady NE,Buckwalter SP,Hall L,Le Febre KM,Binnicker MJ,Wengenack NL

更新日期：2011-09-01 00:00:00

abstract：:The first outbreak caused by vancomycin-resistant enterococci of the VanB phenotype in Poland was analyzed. It occurred in a single ward of a Warsaw hospital which is a specialized center for the treatment of hematological disorders. Between July 1999 and February 2000, 11 patients in the ward were found to be infecte...

journal_title：Journal of clinical microbiology

authors： Kawalec M,Gniadkowski M,Zaleska M,Ozorowski T,Konopka L,Hryniewicz W

更新日期：2001-05-01 00:00:00

abstract：:The use of 1% unmodified rice starch and 1% horse serum instead of 2% soluble starch and 5% serum in Granada medium is described. These components result in a medium of increased stability, preventing spoilage after a few days of storage at room temperature. ...

journal_title：Journal of clinical microbiology

authors： Rosa-Fraile M,Rodríguez-Granger J,Camacho-Muñoz E,Sampedro A

更新日期：2005-04-01 00:00:00

abstract：:We determined the E-Test and National Committee for Clinical Laboratory Standards standardized agar dilution MICs of ceftazidime, ciprofloxacin, piperacillin, and tobramycin for Pseudomonas aeruginosa during tests of 100 rough and mucoid P. aeruginosa isolates from cystic fibrosis patients. The levels of agreement (+/...

journal_title：Journal of clinical microbiology

authors： Marley EF,Mohla C,Campos JM

更新日期：1995-12-01 00:00:00

abstract：:The PNA-FISH Yeast Traffic Light assay was performed on 54 clinical isolates of yeasts inoculated into blood culture bottles. The assay showed high sensitivity (Candida albicans/C. parapsilosis, 100%; C. glabrata/C. krusei, 92.3%; C. tropicalis, 100%) and specificity (C. albicans/C. parapsilosis, 100%; C. glabrata/C. ...

journal_title：Journal of clinical microbiology

authors： Stone NR,Gorton RL,Barker K,Ramnarain P,Kibbler CC

更新日期：2013-04-01 00:00:00

abstract：:In this study, we determined the sequences of four intimin variant genes detected in attaching and effacing Escherichia coli isolates of human origin. Three of them were novel and were designated eae-eta (eta), eae-iota (iota), and eae-kappa (kappa). The fourth was identical to the recently described eae-zeta (zeta), ...

journal_title：Journal of clinical microbiology

authors： Zhang WL,Köhler B,Oswald E,Beutin L,Karch H,Morabito S,Caprioli A,Suerbaum S,Schmidt H

更新日期：2002-12-01 00:00:00

abstract：:In an examination of arginine-utilizing Mycoplasma species, simple fluorogenic and chromogenic tests were used to demonstrate high levels of arginine aminopeptidase activity in unwashed, unconcentrated broth and agar cultures in all strains of 5 species and low levels of activity in all strains of 14 species. Four spe...

journal_title：Journal of clinical microbiology

authors： Ball HJ,Neill SD,Reid RL

更新日期：1985-05-01 00:00:00

abstract：:Trichophyton rubrum was easily differentiated from T. mentagrophytes by its ability to assimilate sorbitol with an API 20C AUX strip. One hundred percent of 36 T. rubrum strains and none of 147 T. mentagrophytes strains assimilated sorbitol. ...

journal_title：Journal of clinical microbiology

authors： Rezusta A,Rubio MC,Alejandre MC

更新日期：1991-01-01 00:00:00

abstract：:In this study, disk diffusion testing with ceftizoxime and cefuroxime was evaluated for use in predicting the susceptibility of Streptococcus pneumoniae to ceftriaxone and cefotaxime. Of the 194 isolates included in this study, 138 were susceptible, 34 were intermediate, and 22 were resistant to cefotaxime by MIC test...

journal_title：Journal of clinical microbiology

authors： Williams-Bouyer N,Hernandez A,Reisner BS

更新日期：1999-11-01 00:00:00

abstract：:Single multiplexed assays could replace the standard 2-tiered (STT) algorithm recommended for the laboratory diagnosis of Lyme disease if they perform with a specificity and a sensitivity superior or equal to those of the STT algorithm. We used human serum rigorously characterized to be sera from patients with acute- ...

journal_title：Journal of clinical microbiology

authors： Arumugam S,Nayak S,Williams T,di Santa Maria FS,Guedes MS,Chaves RC,Linder V,Marques AR,Horn EJ,Wong SJ,Sia SK,Gomes-Solecki M

更新日期：2019-11-22 00:00:00

abstract：:The etiology of Crohn's disease in humans is largely unknown. Clinical signs of Crohn's disease partly resemble the clinical picture of Johne's disease in ruminants caused by Mycobacterium avium subsp. paratuberculosis. Because of the high prevalence of these bacteria in (products of) ruminants and their remarkable th...

journal_title：Journal of clinical microbiology

authors： Overduin P,Schouls L,Roholl P,van der Zanden A,Mahmmod N,Herrewegh A,van Soolingen D

更新日期：2004-11-01 00:00:00