Abstract:
:The sensitivities and specificities of three immunoassays for the detection of herpes simplex virus type 2 (HSV-2)-specific immunoglobulin G antibodies in serum, including the one-strip rapid immunoblot assay (RIBA; Chiron Corporation) and two indirect enzyme immunosorbent assays (EIA; Gull Laboratories and Centocor), were compared by testing a panel of 1,250 serum samples from individuals attending an outpatient clinic for sexually transmitted diseases. A qualitative agreement among the three assays was observed with 1,080 serum samples (86.4%); 291 of the serum samples (23.3%) were positive, 789 samples (63.1%) were negative, and 170 serum samples (13.6%) gave a discordant result. Results were considered conclusive when a concordant result was obtained with two of three assays. The sensitivities and specificities of the RIBA, the Gull EIA, and the Centocor EIA proved to be 99.2, 99.7, and 89.9% and 97.1, 96.7, and 99.3%, respectively. These results indicate that the Chiron RIBA and the Gull EIA are especially useful and reliable for the detection of HSV-2-specific antibodies in serum.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Groen J,Van Dijk G,Niesters HG,Van Der Meijden WI,Osterhaus ADdoi
10.1128/JCM.36.3.845-847.1998subject
Has Abstractpub_date
1998-03-01 00:00:00pages
845-7issue
3eissn
0095-1137issn
1098-660Xjournal_volume
36pub_type
杂志文章abstract::Several unusual fatty acids characterized strains of Francisella tularensis. Long-chain (C20-C26) acids and the hydroxy acids 2-hydroxy-decanoate, 3-hydroxy-hexadecanoate, and 3-hydroxy-octadecanoate appeared to be of special diagnostic value. ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.10.6.928-930.1979
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abstract::The Isolator 10 lysis-centrifugation blood culture system (E. I. du Pont de Nemours & Co., Inc., Wilmington, Del.) was compared with the BACTEC radiometric method (Johnston Laboratories, Inc., Towson, Md.) with 6B and 7D broth media for the recovery of bacteria and yeasts. From 11,000 blood cultures, 1,174 clinically ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.22.6.951-954.1985
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/jcm.41.7.2908-2914.2003
更新日期:2003-07-01 00:00:00
abstract::Depletion of swabs and viral transport medium during epidemics may prompt the use of unvalidated alternatives. Swabs collected and transported dry or in saline were compared to commercially available swab/medium combinations for PCR detection of influenza, enterovirus, herpes simplex virus, and adenovirus. Each was de...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.06551-11
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abstract::We report five new IS711 chromosomal locations that are specific for marine mammal Brucella groups of strains and useful for their identification and classification. Our data support their current classification into two species, Brucella ceti and B. pinnipedialis, with subgroups in each, but also the possibility of a...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01069-10
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abstract::Antisera prepared against the eight recognized serotypes of Ureaplasma urealyticum were tested against the homologous and heterologous antigens by a modified metabolic inhibition test that used bromothymol blue broth in microtiter plates. The method gives clear endpoint determinations which are usually maintained on c...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:
更新日期:1979-06-01 00:00:00
abstract::Family members of hepatitis C virus (HCV)-infected hemodialysis patients had a high rate (8.1%) of HCV infection and were often infected by the same genotype of HCV as the renal patients. Renal patients who had a longer history of hemodialysis or more frequent arteriovenous shunt replacement posed a higher risk to the...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.32.11.2824-2826.1994
更新日期:1994-11-01 00:00:00
abstract::Eight Staphylococcus aureus strains initially identified by Vitek GPS-BS or GPS-SA cards as resistant to oxacillin, but susceptible to most non-beta-lactam antibiotics, were found on further testing to be susceptible to oxacillin and ceftizoxime by disk diffusion tests. For all these strains, the MICs of oxacillin wer...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.37.5.1619-1620.1999
更新日期:1999-05-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.15.5.752-756.1982
更新日期:1982-05-01 00:00:00
abstract::The reported rate of detection of Chlamydia pneumoniae DNA within atherosclerotic lesions by PCR varies between 0 and 100%. In this study, identical sets of coded experimental atheroma samples (n = 15) and spiked controls (n = 5) were analyzed by 16 test methods in nine centers by means of PCR. The positive controls w...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章,多中心研究
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更新日期:2001-02-01 00:00:00
abstract::Chronic periodontitis is a common infectious disease in the adult population. The etiology is clearly bacterial, and a small number of bacterial species have been consistently associated with periodontitis, including Bacteroides forsythus and Porphyromonas gingivalis. Comparatively little attention has been paid to th...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/jcm.40.3.821-825.2002
更新日期:2002-03-01 00:00:00
abstract::Diagnostic laboratories are under increasing pressure to improve and expand their services. Greater flexibility in sample processing is a critical factor that can improve the time to results while reducing reagent waste, making laboratories more efficient and cost-effective. The introduction of the Abbott mPLUS featur...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01672-13
更新日期:2013-12-01 00:00:00
abstract::We previously reported on the efficacy of the enzyme-linked immunoglobulin M capture immune complex (IC) biotinylated antigen assay (EMIBA) for the seroconfirmation of early Lyme disease and active infection with Borrelia burgdorferi. In earlier work we identified non-cross-reacting epitopes of a number of B. burgdorf...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.36.4.1074-1080.1998
更新日期:1998-04-01 00:00:00
abstract::Forty human clinical Mycobacterium avium-M. intracellulare complex strains isolated in Greece were characterized to the species level by PCR with three sets of primers specific for one or both species. M. avium predominated in both human immunodeficiency virus-positive and -negative patients, but the frequency of M. i...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.35.11.3001-3003.1997
更新日期:1997-11-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.02328-10
更新日期:2011-03-01 00:00:00
abstract::During an 8.5-year period (1974 to 1982), 56 Yersinia enterocolitica isolates from patients residing mainly in the New York City area were studied. Evaluation of these isolates revealed a marked increase in bacteremic episodes caused by strains of serogroups of O:3 and O:5,27 and the overall emergence of serogroup O:3...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.17.1.63-67.1983
更新日期:1983-01-01 00:00:00
abstract::Opinions differ on the value of microbiological testing of endoscopes, which varies according to the technique used. We compared the efficacy on bacterial biofilms of sampling solutions used for the surveillance of the contamination of endoscope channels. To compare efficacy, we used an experimental model of a 48-h Ps...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.06221-11
更新日期:2012-03-01 00:00:00
abstract::New blood culture media containing antibiotic-binding polymeric beads have been developed for the BacT/Alert (bioMérieux, Inc., Durham, NC) blood culture system. To assess the performance of these new media, we compared the new BacT/Alert aerobic medium (FA Plus) with resins to BacT/Alert FA medium with activated char...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章,多中心研究
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.32.8.2013-2015.1994
更新日期:1994-08-01 00:00:00
abstract::Over a 12-month period, 43 children in eight different wards of our hospital (Hôpital Robert Debré) were infected or colonized with Klebsiella pneumoniae strains producing extended broad-spectrum beta-lactamases. The epidemiology of the outbreak was studied by a molecular approach including the determination of the be...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.31.2.179-184.1993
更新日期:1993-02-01 00:00:00
abstract::Two previously demonstrated sensitive and specific enzyme immunoassays (EIAs) for rotavirus, one using polyclonal and monoclonal antisera (TestPack Rotavirus [TPK]; Abbott Laboratories) and the other using only monoclonal anti-rotavirus antibodies (Rotaclone [RTC]; Cambridge BioScience Corporation), were evaluated as ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.28.6.1280-1283.1990
更新日期:1990-06-01 00:00:00
abstract::The first outbreak caused by vancomycin-resistant enterococci of the VanB phenotype in Poland was analyzed. It occurred in a single ward of a Warsaw hospital which is a specialized center for the treatment of hematological disorders. Between July 1999 and February 2000, 11 patients in the ward were found to be infecte...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.39.5.1781-1787.2001
更新日期:2001-05-01 00:00:00
abstract::We describe a multiplex PCR assay to identify and discriminate between isolates of Campylobacter coli, Campylobacter jejuni, Campylobacter lari, and Campylobacter upsaliensis. The C. jejuni isolate F38011 lpxA gene, encoding a UDP-N-acetylglucosamine acyltransferase, was identified by sequence analysis of an expressio...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.42.12.5549-5557.2004
更新日期:2004-12-01 00:00:00
abstract::The reliability of an rRNA-derived oligonucleotide probe for Neisseria gonorrhoeae was tested with 187 N. gonorrhoeae isolates, 81 Neisseria meningitidis isolates, and several strains of other bacterial species. The probe proved to be 100% specific and 100% sensitive. N. gonorrhoeae cells could also be reliably identi...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.28.5.944-948.1990
更新日期:1990-05-01 00:00:00
abstract::By using primers based on the sequence of a species-specific antigen of Helicobacter pylori (P. O'Toole, S.M. Logan, M. Kostrzynska. T. Wadström, and T.J. Trust, J. Bacteriol. 173:505-513, 1991), a protocol was established for detection of this microorganism in gastric biopsy samples by the polymerase chain reaction (...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.30.1.54-58.1992
更新日期:1992-01-01 00:00:00
abstract::A simple procedure for the selective isolation of the protective species-specific protein antigens (SPAs) of Rickettsia typhi and Rickettsia prowazekii was developed to permit use of the SPAs in the immunodiagnosis and immunoprophylaxis of typhus infections. Although the SPAs were readily extracted from lysozyme- or d...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.14.3.333-341.1981
更新日期:1981-09-01 00:00:00
abstract::The recently sequenced genome of Campylobacter jejuni RM1221 revealed the presence of three integrated bacteriophage-like elements. In this study, genes from the first element, a Mu-like bacteriophage, were amplified by PCR and used to probe pulsed-field gels of clinical C. jejuni strains obtained from a waterborne ou...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01513-06
更新日期:2007-02-01 00:00:00
abstract::We tested 606 specimens for cytomegalovirus infection. Fifty-two specimens were positive by conventional culture versus forty-three by pre-cytopathic effect detection. Twenty-nine specimens were positive by both methods. Because of toxicity, specimens should be set up on both conventional and shell vial culture system...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.28.4.806-807.1990
更新日期:1990-04-01 00:00:00
abstract::The purpose of this study was to investigate the presence of the anaerobic intestinal spirochetes Brachyspira aalborgi and Brachyspira pilosicoli in the feces of captive nonhuman primates (n = 35) from 19 species housed at the Zoological Gardens, Perth, Western Australia. Both spirochete species are known to infect hu...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/jcm.41.3.1187-1191.2003
更新日期:2003-03-01 00:00:00