Evaluation of an rRNA-derived oligonucleotide probe for culture confirmation of Neisseria gonorrhoeae.

Abstract:

:The reliability of an rRNA-derived oligonucleotide probe for Neisseria gonorrhoeae was tested with 187 N. gonorrhoeae isolates, 81 Neisseria meningitidis isolates, and several strains of other bacterial species. The probe proved to be 100% specific and 100% sensitive. N. gonorrhoeae cells could also be reliably identified in contaminated cultures with the oligonucleotide probe. The 2.6-megadalton cryptic plasmid used as a probe for N. gonorrhoeae was shown to be less sensitive, detecting 179 of 181 N. gonorrhoeae isolates.

journal_name

J Clin Microbiol

authors

Rossau R,Duhamel M,Van Dyck E,Piot P,Van Heuverswyn H

doi

10.1128/JCM.28.5.944-948.1990

subject

Has Abstract

pub_date

1990-05-01 00:00:00

pages

944-8

issue

5

eissn

0095-1137

issn

1098-660X

journal_volume

28

pub_type

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