Clonal reproduction and limited dispersal in an environmental population of Cryptococcus neoformans var gattii isolates from Australia.

abstract：:A comparison of three different commercial media was made to assess their recovery of anaerobic organisms from the blood stream. The three media used were the 50-ml brain heart infusion broth with added CO2 (Pfizer), the 50-ml Thiol broth with added CO2 (Difco), and the 50-ml prereduced, supplemented peptone broth in ...

journal_title：Journal of clinical microbiology

authors： Mangels JI,Lindberg LH,Vosti KL

更新日期：1977-05-01 00:00:00

abstract：:A newly developed commercial serological test (Iatron Laboratories, Inc., Tokyo, Japan) for the rapid identification of medically important species of Candida was evaluated against the API 20C (Analytab Products, Plainview, N.Y.) and the standard Wickerham assimilation and fermentation procedures. Our results indicate...

journal_title：Journal of clinical microbiology

authors： Shinoda T,Kaufman L,Padhye AA

更新日期：1981-03-01 00:00:00

abstract：:A biotyping scheme using improved media and methods for the detection of hippurate hydrolysis, rapid H2S production, and DNA hydrolysis was applied to 1,826 cultures of Campylobacter jejuni, Campylobacter coli and "Campylobacter laridis" isolates from human and nonhuman sources. Four biotypes were identified among C. ...

journal_title：Journal of clinical microbiology

authors： Lior H

更新日期：1984-10-01 00:00:00

abstract：:We have developed a hexaplex PCR assay for rapid detection of the virulence and regulatory genes for cholera toxin enzymatic subunit A (ctxA), zonula occludens toxin (zot), accessory cholera enterotoxin (ace), toxin-coregulated pilus (tcpA), outer membrane protein (ompU), and central regulatory protein ToxR (toxR) in ...

journal_title：Journal of clinical microbiology

authors： Singh DV,Isac SR,Colwell RR

更新日期：2002-11-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay for the measurement of antibodies directed against cell surface antigens of Vibrio cholerae (CSA ELISA) was developed. NaN3-killed whole cells of V. cholerae, adsorbed to polystyrene tubes, were used as immobilized antigens. The assay was capable of detecting antibodies directed ag...

journal_title：Journal of clinical microbiology

authors： Cryz SJ Jr,Fürer E,Germanier R

更新日期：1982-07-01 00:00:00

abstract：:PCR-amplified 16S rRNA gene sequences were obtained directly from tissue specimens from eight cats with presumptive feline leprosy. Acid-fast bacilli were observed in sections from all eight specimens, but culture for mycobacteria was successful for one specimen only. Analysis of the V2 variable region of each 16S rRN...

journal_title：Journal of clinical microbiology

authors： Hughes MS,Ball NW,Beck LA,de Lisle GW,Skuce RA,Neill SD

更新日期：1997-10-01 00:00:00

abstract：:We tested 606 specimens for cytomegalovirus infection. Fifty-two specimens were positive by conventional culture versus forty-three by pre-cytopathic effect detection. Twenty-nine specimens were positive by both methods. Because of toxicity, specimens should be set up on both conventional and shell vial culture system...

journal_title：Journal of clinical microbiology

authors： Rabella N,Drew WL

更新日期：1990-04-01 00:00:00

abstract：:A simple method, using commercial disks to detect Escherichia coli mutator strains, is proposed. The breakpoint for detecting strains with a mutation frequency >/=5 x 10(-7) was established at >/=70 and >/=20 colonies in the inhibition zone of fosfomycin and rifampin disks, respectively, after seeding 100 microl of an...

journal_title：Journal of clinical microbiology

authors： Galán JC,Tato M,Baquero MR,Turrientes C,Baquero F,Martinez JL

更新日期：2004-09-01 00:00:00

abstract：:We compared the performance of a prototype version of the Hybrid Capture 3 (HC3) human papillomavirus (HPV) DNA assay to the current generation Hybrid Capture 2 (HC2) assay, both of which target 13 oncogenic HPV types, for the detection of cervical intraepithelial neoplasia grade 3 and cancer (CIN3+) with cervicovagin...

journal_title：Journal of clinical microbiology

authors： Castle PE,Lorincz AT,Scott DR,Sherman ME,Glass AG,Rush BB,Wacholder S,Burk RD,Manos MM,Schussler JE,Macomber P,Schiffman M

更新日期：2003-09-01 00:00:00

abstract：:Sputum specimens received for the diagnosis of tuberculosis or other mycobacterial infections were tested by a ligase chain reaction (LCR)-based assay and acid-fast stain and culture techniques. Results from the LCR assay (Abbott LCx Mycobacterium tuberculosis [MTB] Assay) were compared to results from standard cultur...

journal_title：Journal of clinical microbiology

authors： Moore DF,Curry JI

更新日期：1998-04-01 00:00:00

abstract：:The polymerase chain reaction (PCR) has many potential applications in the field of nucleic acid diagnostics. In particular, it has been successfully applied to the detection of pathogens present in low copy numbers such as the human immunodeficiency virus type 1. Here we describe a time-resolved fluorescence-based hy...

journal_title：Journal of clinical microbiology

authors： Dahlén PO,Iitiä AJ,Skagius G,Frostell A,Nunn MF,Kwiatkowski M

更新日期：1991-04-01 00:00:00

abstract：:A 115-kDa exoantigen produced by Cryptococcus neoformans recognized by the previously described murine monoclonal antibody 7C9 has been purified from culture filtrate by a combination of membrane ultrafiltration, isoelectric focusing, and preparative gel electrophoresis. It is produced in late-log-phase cultures and i...

journal_title：Journal of clinical microbiology

authors： Hamilton AJ,Goodley J

更新日期：1993-02-01 00:00:00

abstract：:A method is presented for preparing smears for proficiency testing and quality control in acid-fast microscopy. The work was prompted by the increased demand for acid-fast bacilli positive smears with characteristic microscopic appearance and among-smear uniformity. ...

journal_title：Journal of clinical microbiology

authors： Smithwick RW,Stratigos CB

更新日期：1978-07-01 00:00:00

abstract：:A case of pyogenic liver abscess (PLA) due to Rhodococcus equi in an immunocompetent individual was successfully treated by combining surgery and antibiotics. The R. equi-targeted antimicrobial agents erythromycin and rifampin were used only after surgical resection of the lesion and identification of the infective or...

journal_title：Journal of clinical microbiology

authors： Napoleão F,Damasco PV,Camello TC,do Vale MD,de Andrade AF,Hirata R Jr,de Mattos-Guaraldi AL

更新日期：2005-02-01 00:00:00

abstract：:Samples of cerebrospinal fluid from 103 patients with aseptic meningitis were tested by PCR for detection of leptospires, and the results were compared with those of the microscopic agglutination test (MAT) and an enzyme-linked immunosorbent assay for detection of immunoglobulin M (ELISA-IgM). Of these samples, 39.80%...

journal_title：Journal of clinical microbiology

authors： Romero EC,Billerbeck AE,Lando VS,Camargo ED,Souza CC,Yasuda PH

更新日期：1998-05-01 00:00:00

abstract：:Since the introduction of antiviral compounds such as lamivudine and famciclovir in the treatment schedules of patients with chronic hepatitis B virus (HBV) infection, the accumulation of a variety of mutations in the HBV polymerase gene has been observed. The selection of these mutations is generally considered the c...

journal_title：Journal of clinical microbiology

authors： Stuyver L,Van Geyt C,De Gendt S,Van Reybroeck G,Zoulim F,Leroux-Roels G,Rossau R

更新日期：2000-02-01 00:00:00

abstract：:A specific PCR for the detection of a variant of the gene encoding Shiga toxin 1 (stx(1)) called stx(1(OX3)) (GenBank accession no. Z36901) was developed. The PCR was used to investigate 148 Stx(1)-producing Escherichia coli strains from human patients (n = 72), cattle (n = 27), sheep (n = 48), and a goat (n = 1) for ...

journal_title：Journal of clinical microbiology

authors： Koch C,Hertwig S,Lurz R,Appel B,Beutin L

更新日期：2001-11-01 00:00:00

abstract：:The new commercial assay GenoType Mycobacterium, intended for the identification of mycobacteria, was evaluated with a panel of 197 strains belonging to 86 different taxa. The system, which relies on solid-phase reverse hybridization, is composed of two different kits: GenoType CM for the identification of more freque...

journal_title：Journal of clinical microbiology

authors： Russo C,Tortoli E,Menichella D

更新日期：2006-02-01 00:00:00

abstract：:We investigated the potential of pooling DNA from nasopharyngeal specimens to reduce the cost of real-time PCR (RT-PCR) for bacterial detection. Lyophilization is required to reconcentrate DNA. This strategy yields a high specificity (86%) and a high sensitivity (96%). We estimate that compared to individual testing, ...

journal_title：Journal of clinical microbiology

authors： Edouard S,Prudent E,Gautret P,Memish ZA,Raoult D

更新日期：2015-03-01 00:00:00

abstract：:The actinomycete Rhodococcus equi is an important pathogen of horses and an emerging opportunistic pathogen of humans. Identification of R. equi by classical bacteriological techniques is sometimes difficult, and misclassification of an isolate is not uncommon. We report here on a specific PCR assay for the rapid and ...

journal_title：Journal of clinical microbiology

authors： Ladrón N,Fernández M,Agüero J,González Zörn B,Vázquez-Boland JA,Navas J

更新日期：2003-07-01 00:00:00

abstract：:We report transmission of an azole-resistant, isogenic strain of Candida albicans in a human immunodeficiency virus (HIV)-infected family of two children with symptomatic oropharyngeal candidiasis and a mother with asymptomatic colonization over a 5-year period. These findings were confirmed by three different molecul...

journal_title：Journal of clinical microbiology

authors： Müller FM,Kasai M,Francesconi A,Brillante B,Roden M,Peter J,Chanock SJ,Walsh TJ

更新日期：1999-10-01 00:00:00

abstract：:INTRODUCTIONSyndromic panels were first FDA cleared for detection of respiratory pathogens in 2008. Since then, other panels have been approved by the FDA, and most recently, the FilmArray meningitis/encephalitis panel (BioFire, Salt Lake City, UT) has become available. This assay detects 14 targets within 1 h and inc...

journal_title：Journal of clinical microbiology

authors： Dien Bard J,Alby K

更新日期：2018-03-26 00:00:00

abstract：:During the course of a molecular epidemiology study of mechanisms of antibiotic resistance in the area served by our hospital (516,000 inhabitants), we isolated the gene encoding CTX-M-14 beta-lactamase. Thirty clinical strains (27 Escherichia coli and 3 Klebsiella pneumoniae isolates) with a phenotype of extended-spe...

journal_title：Journal of clinical microbiology

authors： Bou G,Cartelle M,Tomas M,Canle D,Molina F,Moure R,Eiros JM,Guerrero A

更新日期：2002-11-01 00:00:00

abstract：:Restriction endonucleases BamHI, BclI, BglI, BglII, BstEII, EcoRI, HindIII, HpaI, SalI, SmalI, XbalI, and XholI were used to analyze 61 selected strains of adenovirus type 3 (Ad3) isolated from Africa, Asia, Australia, Europe, North America, and South America. It was noted that the use of BamHI, BclI, BglII, HpaI, Sal...

journal_title：Journal of clinical microbiology

authors： Li QG,Wadell G

更新日期：1988-05-01 00:00:00

abstract：:The Porphyromonas gingivalis collagenase-specific serum immunoglobulin A (IgA), IgM, and IgG responses from 20 patients with early-onset periodontitis (EOP), 20 patients with adult periodontitis, (AP), and 20 age- and sex-matched healthy controls were examined by immunoblot analysis. A recombinant collagenase antigen ...

journal_title：Journal of clinical microbiology

authors： Wittstock M,Flemmig TF,Schmidt H,Mutters R,Karch H

更新日期：1996-10-01 00:00:00

abstract：:The p44 gene of the agent of human granulocytic ehrlichiosis (aoHGE) encodes a 44-kDa major outer surface protein. A technique was developed for the typing of the aoHGE based on the PCR amplification of the p44 gene followed by a multiple restriction digest with HindIII, EcoRV, and AspI to generate restriction fragmen...

journal_title：Journal of clinical microbiology

authors： Carter SE,Ravyn MD,Xu Y,Johnson RC

更新日期：2001-09-01 00:00:00

abstract：:Six patients with bone marrow cultures yielding Mycobacterium avium complex were encountered at the Mount Sinai Hospital between 1969 and 1976. One additional isolate of the same mycobacterial species was recovered from splenic cyst fluid of a seventh patient. Because none of the patients had illnesses apparently due ...

journal_title：Journal of clinical microbiology

authors： Kozinn WP,Damsker B,Bottone EJ

更新日期：1980-03-01 00:00:00

abstract：:Resistance to extended-spectrum β-lactam antibiotics has led to a greater reliance upon carbapenems, but the expression of carbapenemases threatens to limit the utility of these drugs. Current methods to detect carbapenemase activity are suboptimal, requiring prolonged incubations during which ineffective therapy may ...

journal_title：Journal of clinical microbiology

authors： Kulkarni MV,Zurita AN,Pyka JS,Murray TS,Hodsdon ME,Peaper DR

更新日期：2014-07-01 00:00:00

abstract：:The medium described is a simple yeast extract broth capable of growing large number of Legionella neumophila, the causative organism of Legionnaires disease. Filtration was chosen as a means of sterilization, since medium that was autoclaved did not support growth without the presence of Norite A. The filtered medium...

journal_title：Journal of clinical microbiology

authors： Ristroph JD,Hedlund KW,Allen RG

更新日期：1980-01-01 00:00:00

abstract：:Direct enzyme immunoassay (Herpchek) was compared with culture on 21,522 specimens mainly from asymptomatic women for herpes simplex virus detection. Sensitivity and specificity were 73.8 and 97.7%, respectively. The 33% detection rate by enzyme immunoassay in 5 h increased to 43% when the enzyme immunoassay was combi...

journal_title：Journal of clinical microbiology

authors： Verano L,Michalski FJ

更新日期：1995-05-01 00:00:00