Detection of Leptospira DNA in patients with aseptic meningitis by PCR.

Abstract:

:Samples of cerebrospinal fluid from 103 patients with aseptic meningitis were tested by PCR for detection of leptospires, and the results were compared with those of the microscopic agglutination test (MAT) and an enzyme-linked immunosorbent assay for detection of immunoglobulin M (ELISA-IgM). Of these samples, 39.80% were positive by PCR and 8.74 and 3.88% were positive by MAT and ELISA-IgM, respectively.

journal_name

J Clin Microbiol

authors

Romero EC,Billerbeck AE,Lando VS,Camargo ED,Souza CC,Yasuda PH

doi

10.1128/JCM.36.5.1453-1455.1998

subject

Has Abstract

pub_date

1998-05-01 00:00:00

pages

1453-5

issue

5

eissn

0095-1137

issn

1098-660X

journal_volume

36

pub_type

杂志文章
  • Clinical and molecular analysis of extended-spectrum {beta}-lactamase-producing enterobacteria in the community setting.

    abstract::During a previous survey, five extended-spectrum beta-lactamase (ESBL)-producing enterobacteria (ESBLE) (two Enterobacter aerogenes isolates expressing TEM-24 b, two Escherichia coli isolates expressing TEM-21 or TEM-24 b, and one Klebsiella pneumoniae isolate expressing SHV-4/TEM-15) responsible for urinary tract inf...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.43.10.5048-5054.2005

    authors: Arpin C,Dubois V,Maugein J,Jullin J,Dutilh B,Brochet JP,Larribet G,Fischer I,Quentin C

    更新日期:2005-10-01 00:00:00

  • Bacteriological and serological findings in a further case of transfusion-mediated Yersinia enterocolitica sepsis.

    abstract::A 13-year-old patient developed severe shock due to administration of a Yersinia enterocolitica-contaminated red blood cell concentrate. Y. enterocolitica (serotype O:9, biotype II) was cultivated from the residual blood in the blood bag and from a stool sample of the blood donor. In the donor's plasma immunoglobulin ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.38.7.2788-2790.2000

    authors: Strobel E,Heesemann J,Mayer G,Peters J,Müller-Weihrich S,Emmerling P

    更新日期:2000-07-01 00:00:00

  • Characteristics and biotypes of Pasteurella multocida isolated from humans.

    abstract::Fifty-two isolates of Pasteurella (48 strains of Pasteurella multocida and 4 strains of atypical Pasteurella) were identified by conventional and commercial test systems. All strains fermented glucose, sucrose, and fructose in purple broth base (Difco Laboratories) with bromocresol purple as indicator, although the at...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.13.3.566-571.1981

    authors: Oberhofer TR

    更新日期:1981-03-01 00:00:00

  • Restriction fragment length polymorphism analysis of 16S ribosomal DNA of Streptococcus and Enterococcus species of bovine origin.

    abstract::Twelve bacterial species including Streptococcus uberis, S. parauberis, S. agalactiae, S. dysgalactiae, S. bovis, S. mitis, S. salivarius, S. saccharolyticus, Enterococcus faecium, E. faecalis, E. avium, and Aerococcus viridans were examined for their 16S ribosomal DNA fingerprint patterns. Oligonucleotide primers com...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.30.9.2235-2240.1992

    authors: Jayarao BM,Doré JJ Jr,Oliver SP

    更新日期:1992-09-01 00:00:00

  • Decreased Seroreactivity in Individuals Initiating Antiretroviral Therapy during Acute HIV Infection.

    abstract::Antiretroviral therapy (ART) during acute HIV infection (AHI) interrupts viral dynamics and may delay the emergence of serological markers targeted by current HIV screening and confirmatory assays, thus creating challenges for correctly classifying HIV infection status. The performance of three HIV antigen/antibody co...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00757-19

    authors: Manak MM,Jagodzinski LL,Shutt A,Malia JA,Leos M,Ouellette J,Akapirat S,Colby DL,Phanuphak N,Eller LA,Robb ML,de Souza M,Ananworanich J,Peel SA,RV254\/SEARCH010 and the RV217 Study Teams.

    更新日期:2019-09-24 00:00:00

  • Prevalence of thymidine-dependent Staphylococcus aureus in patients with cystic fibrosis.

    abstract::During a 1-year period, the prevalence of thymidine-dependent (TD) Staphylococcus aureus in patients at two geographically distinct cystic fibrosis (CF) centers was determined. Of 200 CF patients who had their respiratory secretions cultured, 95 harbored S. aureus, and 20 (21%) had TD S. aureus as their predominant st...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.25.7.1258-1261.1987

    authors: Gilligan PH,Gage PA,Welch DF,Muszynski MJ,Wait KR

    更新日期:1987-07-01 00:00:00

  • Analysis of pathogen and host factors related to clinical outcomes in patients with hospital-acquired pneumonia due to methicillin-resistant Staphylococcus aureus.

    abstract::Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of nosocomial pneumonia. To characterize pathogen-derived and host-related factors in intensive care unit (ICU) patients with MRSA pneumonia, we evaluated the Improving Medicine through Pathway Assessment of Critical Therapy in Hospital-Acquired Pneum...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.06701-11

    authors: Haque NZ,Arshad S,Peyrani P,Ford KD,Perri MB,Jacobsen G,Reyes K,Scerpella EG,Ramirez JA,Zervos MJ

    更新日期:2012-05-01 00:00:00

  • Prosthetic joint infection caused by Helcococcus kunzii.

    abstract::Helcococcus kunzii was isolated by sonication and conventional cultures obtained from a case of infection following total knee prosthesis in an immunocompetent patient. The patient recovered uneventfully. This is the first known case of an H. kunzii prosthetic joint infection. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01244-11

    authors: Pérez-Jorge C,Cordero J,Marin M,Esteban J

    更新日期:2012-02-01 00:00:00

  • Molecular epidemiology of clinical isolates of methicillin-resistant Staphylococcus aureus in Taiwan.

    abstract::During July 2000 and October 2001, a total of 595 clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) were collected from six medical centers distributed in northern, central, and southern Taiwan. Specimen sources included blood (n = 279), pus (n = 173), sputum (n = 94), body fluids (n = 21), cathe...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.42.1.307-310.2004

    authors: Huang YC,Su LH,Wu TL,Liu CE,Young TG,Chen PY,Hseuh PR,Lin TY

    更新日期:2004-01-01 00:00:00

  • Enterohemorrhagic Escherichia coli (EHEC) strains of serogroup O118 display three distinctive clonal groups of EHEC pathogens.

    abstract::A recent case report of a child infected with enterohemorrhagic Escherichia coli (EHEC) of serotype O118:H16 in Bavaria, in association with the isolation of a bovine O118 strain on the same farm (A. Weber, H. Klie, H. Richter, P. Gallien, M. Timm, and K. W. Perlberg, Berl. Muench. Tieraerztl. Wochenschr. 110:211-213,...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:

    authors: Wieler LH,Busse B,Steinrück H,Beutin L,Weber A,Karch H,Baljer G

    更新日期:2000-06-01 00:00:00

  • Canada's first case of a multidrug-resistant Corynebacterium diphtheriae strain, isolated from a skin abscess.

    abstract::A toxigenic Corynebacterium diphtheriae biovar mitis sequence type 136 (ST136) strain was recovered from a toe infection of an unvaccinated patient recently returned from India. The isolate was resistant to clindamycin, erythromycin (ermX positive), tetracycline, and trimethoprim-sulfamethoxazole, intermediate to ceft...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.05296-11

    authors: Mina NV,Burdz T,Wiebe D,Rai JS,Rahim T,Shing F,Hoang L,Bernard K

    更新日期:2011-11-01 00:00:00

  • Seronegative hepatitis C virus infection in a child infected via mother-to-child transmission.

    abstract::Hepatitis C virus (HCV) infection typically leads to antibody response within weeks after primary infection. Here, we describe the case of a child infected with HCV by mother-to-child transmission who remained persistently seronegative despite the presence of high levels of circulating HCV RNA. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00622-12

    authors: Larouche A,Gaëtan G,El-Bilali N,Quesnel-Vallières M,Martin SR,Alvarez F,Shoukry NH,Soudeyns H

    更新日期:2012-07-01 00:00:00

  • Aeration selects for mucoid phenotype of Pseudomonas aeruginosa.

    abstract::A mucoid strain of Pseudomonas aeruginosa isolated from a patient with cystic fibrosis and its nonmucoid revertant were grown in a chemically defined alginate-promoting medium under batch and continuous culture conditions. Selection for the mucoid and nonmucoid phenotype was accomplished by varying the levels of air a...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.24.6.986-990.1986

    authors: Krieg DP,Bass JA,Mattingly SJ

    更新日期:1986-12-01 00:00:00

  • Polymerase chain reaction analyses identify two distinct classes of Borrelia burgdorferi.

    abstract::We sequenced homologous chromosomal loci from several North American and European isolates of the Lyme disease spirochete Borrelia burgdorferi, as well as from the relapsing fever spirochete Borrelia hermsii. Inter- and intraspecies sequence comparisons permitted the design of B. burgdorferi-specific polymerase chain ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.29.3.524-532.1991

    authors: Rosa PA,Hogan D,Schwan TG

    更新日期:1991-03-01 00:00:00

  • Rapid detection and identification of mucormycetes from culture and tissue samples by use of high-resolution melt analysis.

    abstract::We present a method for rapid and simple detection of clinically relevant mucormycetes of the Mucorales order in cultures and clinical samples. This seminested real-time PCR uses mucormycete-specific primers and is followed by species identification using high-resolution melt (HRM) analysis. The method is highly suita...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01109-10

    authors: Hrncirova K,Lengerova M,Kocmanova I,Racil Z,Volfova P,Palousova D,Moulis M,Weinbergerova B,Winterova J,Toskova M,Pospisilova S,Mayer J

    更新日期:2010-09-01 00:00:00

  • Gene mutations responsible for overexpression of AmpC beta-lactamase in some clinical isolates of Enterobacter cloacae.

    abstract::AmpC regulatory genes in 21 ceftazidime-resistant clinical isolates of Enterobacter cloacae (MICs of > or = 16 microg/ml) were characterized. All isolates exhibited AmpC overproduction due to AmpD mutation. Additionally, we found two AmpR mutants among the isolates. This is the first report of chromosomal ampR mutatio...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.43.6.2955-2958.2005

    authors: Kaneko K,Okamoto R,Nakano R,Kawakami S,Inoue M

    更新日期:2005-06-01 00:00:00

  • Characterization of group C rotaviruses associated with diarrhea outbreaks in feeder pigs.

    abstract::Feces and serum specimens were collected from three farms in Michigan on which approximately 50-lb (8- to 9-week-old) pigs experienced diarrhea just after placement into all-in-all-out finishing barns. The clinical signs (profuse watery diarrhea lasting about 2 weeks and no vomiting) were similar on all farms, and the...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.37.5.1484-1488.1999

    authors: Kim Y,Chang KO,Straw B,Saif LJ

    更新日期:1999-05-01 00:00:00

  • Isolation of an unusual mycobacterium from an AIDS patient.

    abstract::A mycobacterium isolated from a clinical sample of an AIDS patient was identified as Mycobacterium interjectum by direct 16S rRNA sequence determination. High-performance liquid chromatography, however, revealed a mycolic acid pattern which was different from the one shared by the previously analyzed strains of this s...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.34.9.2316-2319.1996

    authors: Tortoli E,Kirschner P,Bartoloni A,Burrini C,Manfrin V,Mantella A,Scagnelli M,Scarparo C,Simonetti MT,Böttger EC

    更新日期:1996-09-01 00:00:00

  • Evaluation of the Vidas Chlamydia test to detect and verify Chlamydia trachomatis in urogenital specimens.

    abstract::The Vidas Chlamydia test (CHL) is an automated enzyme-linked immunofluorescence assay for the detection of Chlamydia trachomatis. Positive and equivocal results are confirmed with a blocking assay. A mouse monoclonal antibody directed against the chlamydial lipopolysaccharides was used for the test. The CHL assay is w...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,多中心研究

    doi:10.1128/JCM.35.8.2102-2106.1997

    authors: Schachter J,Jones RB,Butler RC,Rice B,Brooks D,Van der Pol B,Gray M,Moncada J

    更新日期:1997-08-01 00:00:00

  • Evaluation of Streck tissue fixative, a nonformalin fixative for preservation of stool samples and subsequent parasitologic examination.

    abstract::We undertook a study to evaluate Streck tissue fixative (STF) as a substitute for formalin and polyvinyl alcohol (PVA) in fecal preservation. A comparison of formalin, PVA, (mercuric chloride based), and STF was done by aliquoting fecal samples into each fixative. Stool specimens were collected in Haiti, and parasites...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.37.12.4113-4119.1999

    authors: Nace EK,Steurer FJ,Eberhard ML

    更新日期:1999-12-01 00:00:00

  • Mycobacterial testing in clinical laboratories that participate in the College of American Pathologists' Mycobacteriology E survey: results of a 1993 questionnaire.

    abstract::Participants in the College of American Pathologists' Mycobacteriology E proficiency testing survey in 1993 were asked to complete a questionnaire addressing mycobacterial test methods, test volume, and frequency of detection of drug-resistant Mycobacterium tuberculosis (MTB). A similar questionnaire had been distribu...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.33.2.407-412.1995

    authors: Woods GL,Witebsky FG

    更新日期:1995-02-01 00:00:00

  • Serodiagnosis of rabies by dot immunobinding assay.

    abstract::A dot immunobinding assay that uses inactivated antigen for the detection of rabies viral antibodies was compared with the rapid fluorescent focus inhibition test. Results of testing pre- and postvaccination sera from humans (n = 33) and canines (n = 22) were identical for both tests. Endpoint titers of positive sera ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.25.7.1262-1264.1987

    authors: Heberling RL,Kalter SS,Smith JS,Hildebrand DG

    更新日期:1987-07-01 00:00:00

  • Molecular epidemiology of multiresistant Streptococcus pneumoniae with both erm(B)- and mef(A)-mediated macrolide resistance.

    abstract::Of a total of 1043 macrolide-resistant Streptococcus pneumoniae isolates collected from 24 countries as part of PROTEKT 1999-2000, 71 isolates tested positive for both the mef(A) and erm(B) genes. Of 69 isolates subjected to further molecular investigations, all were resistant to tetracycline, 63 (91.3%) were resistan...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.42.2.764-768.2004

    authors: Farrell DJ,Morrissey I,Bakker S,Morris L,Buckridge S,Felmingham D

    更新日期:2004-02-01 00:00:00

  • Characterization of some actinomyces-like isolates from human clinical sources: description of Varibaculum cambriensis gen nov, sp nov.

    abstract::Fifteen strains of an anaerobic, catalase-negative, gram-positive diphtheroid-shaped bacterium recovered from human sources were characterized by phenotypic and molecular chemical and molecular genetic methods. The unidentified bacterium showed some resemblance to Actinomyces species and related taxa, but biochemical ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.41.2.640-644.2003

    authors: Hall V,Collins MD,Lawson PA,Hutson RA,Falsen E,Inganas E,Duerden B

    更新日期:2003-02-01 00:00:00

  • Purification of sputum samples through sucrose improves detection of Mycobacterium tuberculosis by polymerase chain reaction.

    abstract::A method is described for the routine preparation of sputum samples for detection of Mycobacterium tuberculosis by using polymerase chain reaction amplification. Liquefaction of sputum samples with NaOH and subsequent removal of inhibitors of the polymerase reaction with a 50% sucrose centrifugation step (5 min) in a ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.30.6.1514-1517.1992

    authors: Victor T,du Toit R,van Helden PD

    更新日期:1992-06-01 00:00:00

  • Serological cross-reaction between Legionella spp. and Capnocytophaga ochracea by using latex agglutination test.

    abstract::Cross-reactivity between Legionella spp. and Capnocytophaga ochracea was noted by latex agglutination tests (Serobact Legionella; Disposable Products, Adelaide, Australia). Four of 11 (36%) C. ochracea isolates agglutinated with latex reagents designed to identify Legionella pneumophila serogroups. C. ochracea isolate...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.32.12.3054-3055.1994

    authors: Chen S,Hicks L,Yuen M,Mitchell D,Gilbert GL

    更新日期:1994-12-01 00:00:00

  • Multifocal detection of multidrug-resistant Pseudomonas aeruginosa producing the PER-1 extended-spectrum beta-lactamase in Northern Italy.

    abstract::Forty-four nonreplicate clinical isolates of Pseudomonas aeruginosa that were resistant to extended-spectrum cephalosporins (ceftazidime and cefepime) and aztreonam, that putatively produced an acquired extended- spectrum beta-lactamase (ESBL), according to the results of a double-disk synergy test, and that had been ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.42.6.2523-2529.2004

    authors: Pagani L,Mantengoli E,Migliavacca R,Nucleo E,Pollini S,Spalla M,Daturi R,Romero E,Rossolini GM

    更新日期:2004-06-01 00:00:00

  • Comparison of PCR-ribotyping, arbitrarily primed PCR, and pulsed-field gel electrophoresis for typing Clostridium difficile.

    abstract::Clostridium difficile is now recognized as the major agent responsible for nosocomial diarrhea in adults. Among the genotyping methods available, arbitrarily primed PCR (AP-PCR), PCR-ribotyping, and pulsed-field gel electrophoresis (PFGE) have been widely used for investigating outbreaks of C. difficile infections. Ho...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.38.7.2484-2487.2000

    authors: Bidet P,Lalande V,Salauze B,Burghoffer B,Avesani V,Delmée M,Rossier A,Barbut F,Petit JC

    更新日期:2000-07-01 00:00:00

  • Association of coagulase-negative staphylococcal slime production and adherence with the development and outcome of adult septicemias.

    abstract::The relationship of coagulase-negative staphylococcal slime production and adherence with the development and outcome of bloodstream infections in two Finnish hospitals was evaluated. Analysis of 64 strains from 62 adult septicemias disclosed 34 (53%) adherent slime producers. In comparison, only 142 (29%) of 489 sing...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.28.12.2779-2785.1990

    authors: Kotilainen P

    更新日期:1990-12-01 00:00:00

  • COVID-19-Associated Invasive Aspergillosis: Data from the UK National Mycology Reference Laboratory.

    abstract::COVID-19-associated pulmonary aspergillosis (CAPA) was recently reported as a potential infective complication affecting critically ill patients with acute respiratory distress syndrome following severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, with incidence rates varying from 8 to 33% dependin...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02136-20

    authors: Borman AM,Palmer MD,Fraser M,Patterson Z,Mann C,Oliver D,Linton CJ,Gough M,Brown P,Dzietczyk A,Hedley M,McLachlan S,King J,Johnson EM

    更新日期:2020-12-17 00:00:00