Characterization of some actinomyces-like isolates from human clinical sources: description of Varibaculum cambriensis gen nov, sp nov.

Abstract:

:Fifteen strains of an anaerobic, catalase-negative, gram-positive diphtheroid-shaped bacterium recovered from human sources were characterized by phenotypic and molecular chemical and molecular genetic methods. The unidentified bacterium showed some resemblance to Actinomyces species and related taxa, but biochemical testing, polyacrylamide gel electrophoresis analysis of whole-cell proteins, and amplified 16S ribosomal DNA restriction analysis indicated the strains were distinct from all currently named Actinomyces species and related taxa. Comparative 16S rRNA gene sequencing studies showed that the bacterium represents a hitherto-unknown phylogenetic line that is related to but distinct from Actinomyces, Actinobaculum, Arcanobacterium, and Mobiluncus: We propose, on the basis of phenotypic and phylogenetic evidence, that the unknown bacterium from human clinical specimens should be classified as a new genus and species, Varibaculum cambriensis gen. nov., sp. nov. The type strain of Varibaculum cambriensis sp. nov. is CCUG 44998(T) = CIP 107344(T).

journal_name

J Clin Microbiol

authors

Hall V,Collins MD,Lawson PA,Hutson RA,Falsen E,Inganas E,Duerden B

doi

10.1128/jcm.41.2.640-644.2003

subject

Has Abstract

pub_date

2003-02-01 00:00:00

pages

640-4

issue

2

eissn

0095-1137

issn

1098-660X

journal_volume

41

pub_type

杂志文章
  • New selective agar medium for isolation of virulent Yersinia enterocolitica.

    abstract::A selective agar medium for isolation of virulent Yersinia enterocolitica (VYE agar) was developed for the rapid and accurate isolation of virulent Y. enterocolitica from environmental samples highly contaminated with environmental Yersinia organisms, as well as for isolation from clinical specimens. VYE agar provided...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.25.6.1068-1073.1987

    authors: Fukushima H

    更新日期:1987-06-01 00:00:00

  • Role of a cefoxitin-inducible beta-lactamase in a case of breakthrough bacteremia.

    abstract::Development of resistance during therapy with cefamandole contributes to treatment failure. A simple cefoxitin disk test was recently described which detects a cefamandole-active inducible beta-lactamase not otherwise detectable with cefamandole as the inducer. A case of breakthrough Enterobacter bacteremia due to se...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.12.4.517-520.1980

    authors: Beckwith DG,Jahre JA

    更新日期:1980-10-01 00:00:00

  • Etiology of sporadic cases of pediatric acute gastroenteritis in asturias, Spain, and genotyping and characterization of norovirus strains involved.

    abstract::From November 2000 to October 2001, a reverse transcription-PCR using primers directed to the norovirus RNA polymerase coding region was included in a viral and bacterial routine screening to diagnose sporadic cases of acute gastroenteritis among children in Asturias, Spain. The role of noroviruses (8.6% of the positi...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.42.6.2668-2674.2004

    authors: Boga JA,Melón S,Nicieza I,De Diego I,Villar M,Parra F,De Oña M

    更新日期:2004-06-01 00:00:00

  • Reverse transcriptase PCR diagnostic assay for the coronavirus associated with severe acute respiratory syndrome.

    abstract::Recent outbreaks of severe acute respiratory syndrome (SARS) have spurred intense research efforts around the world to deal with the serious threat to health posed by this novel coronavirus. A rapid, reliable diagnostic assay is needed for monitoring the spread of the disease. Here we report a method for eliminating f...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.42.5.1994-1999.2004

    authors: Hui RK,Zeng F,Chan CM,Yuen KY,Peiris JS,Leung FC

    更新日期:2004-05-01 00:00:00

  • Multicenter Comparison Study of both Analytical and Clinical Performance across Four Roche Hepatitis C Virus RNA Assays Utilizing Different Platforms.

    abstract::The efficacy of antiviral treatment for chronic hepatitis C virus (HCV) infection is determined by measuring HCV RNA at specific time points throughout therapy using highly sensitive and accurate HCV RNA assays. This study compared the performances of two recently developed real-time PCR HCV RNA assays, cobas HCV for ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,多中心研究

    doi:10.1128/JCM.02193-16

    authors: Vermehren J,Stelzl E,Maasoumy B,Michel-Treil V,Berkowski C,Marins EG,Paxinos EE,Marino E,Wedemeyer H,Sarrazin C,Kessler HH

    更新日期:2017-04-01 00:00:00

  • Latex agglutination test for monitoring antibodies to avian influenza virus subtype H5N1.

    abstract::A latex agglutination test (LAT) based on polystyrene beads sensitized with inactivated avian influenza virus H5N1 particles was developed. Compared with the hemagglutination inhibition test, the sensitivity and specificity of the LAT were 88.8 and 97.6%, respectively, in detecting 830 serum samples from vaccinated ch...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.43.4.1953-1955.2005

    authors: Xu X,Jin M,Yu Z,Li H,Qiu D,Tan Y,Chen H

    更新日期:2005-04-01 00:00:00

  • Identification of Burkholderia pseudomallei by Use of the Vitek Mass Spectrometer.

    abstract::Burkholderia pseudomallei is the causative agent of melioidosis. This condition most often presents as pneumonia and bacteremia, with mortality rates of 9% to 70%. Therefore, early identification of this organism may aid in directing appropriate management. This study aimed to use the Vitek matrix-assisted laser desor...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00081-19

    authors: Gassiep I,Armstrong M,Norton RE

    更新日期:2019-04-26 00:00:00

  • Simultaneous detection of immunoglobulin A (IgA) and IgM antibodies against hepatitis E virus (HEV) Is highly specific for diagnosis of acute HEV infection.

    abstract::Serum samples collected from 68 patients (age, mean +/- the standard deviation [SD], 56.3 +/- 12.8 years) at admission who were subsequently molecularly diagnosed as having hepatitis E and from 2,781 individuals who were assumed not to have been recently infected with hepatitis E virus (HEV; negative controls; 52.9 +/...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.43.1.49-56.2005

    authors: Takahashi M,Kusakai S,Mizuo H,Suzuki K,Fujimura K,Masuko K,Sugai Y,Aikawa T,Nishizawa T,Okamoto H

    更新日期:2005-01-01 00:00:00

  • Identification of serotype 9 human rotavirus by enzyme-linked immunosorbent assay with monoclonal antibodies.

    abstract::Hybridomas producing monoclonal antibodies to VP7, a major neutralizing protein of serotype 9 rotavirus (strain W161), were prepared. One monoclonal antibody, W161-6A1, was shown to neutralize only serotype 9 rotavirus strains and reacted specifically with serotype 9 rotaviruses in an enzyme-linked immunosorbent assay...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.27.9.2112-2114.1989

    authors: Midthun K,Valdesuso J,Kapikian AZ,Hoshino Y,Green KY

    更新日期:1989-09-01 00:00:00

  • Susceptibility testing of fungi: current status of correlation of in vitro data with clinical outcome.

    abstract::In summary, it is clear that in vitro susceptibility testing can predict outcome in selected clinical situations. The clearest data are from the fluconazole-treated AIDS patients with oropharyngeal candidiasis. In this setting, the homogeneity of the underlying immune defect, combined with the ease of identification a...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,评审

    doi:10.1128/JCM.34.3.489-495.1996

    authors: Ghannoum MA,Rex JH,Galgiani JN

    更新日期:1996-03-01 00:00:00

  • An unusual phagocytosis: ingestion of a Giardia lamblia cyst by an Entamoeba coli trophozoite.

    abstract::Microscopic examination of a permanently stained fecal preparation revealed the unusual inclusion of a Giardia lamblia cyst within the cytoplasm of an Entamoeba coli trophozoite. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.12.4.606-607.1980

    authors: Logan AL,Turner WE,Price J Jr

    更新日期:1980-10-01 00:00:00

  • A Public Health Laboratory Response to the Pandemic.

    abstract::An outbreak of coronavirus disease 2019 (COVID-19) caused by a novel coronavirus (severe acute respiratory syndrome coronavirus 2 [SARS-CoV-2]) began in Wuhan, Hubei, China, in December 2019 and spread rapidly worldwide. The response by the Alberta Precision Laboratories, Public Health Laboratory (ProvLab), AB, Canada...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01110-20

    authors: Pabbaraju K,Wong AA,Douesnard M,Ma R,Gill K,Dieu P,Fonseca K,Zelyas N,Tipples GA

    更新日期:2020-07-23 00:00:00

  • Degenerate and nested PCR: a highly sensitive and specific method for detection of human papillomavirus infection in cutaneous warts.

    abstract::The role of human papillomavirus (HPV) in anogenital carcinogenesis is firmly established, but evidence that supports a similar role in skin remains speculative. Immunosuppressed renal transplant recipients have an increased incidence of viral warts and nonmelanoma skin cancer, and the presence of HPV DNA in these les...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.37.11.3545-3555.1999

    authors: Harwood CA,Spink PJ,Surentheran T,Leigh IM,de Villiers EM,McGregor JM,Proby CM,Breuer J

    更新日期:1999-11-01 00:00:00

  • Analysis of GB virus C markers in families over three generations.

    abstract::GB virus C (GBV-C) markers were analyzed in two to three generations in three families with documented vertical transmission of GBV-C. None of the maternal grandparents had GBV-C markers, whereas the male spouses had GBV-C envelope 2 antibodies. Evidence was found for intrafamilial transmission but not for GBV-C trans...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.37.12.4153-4155.1999

    authors: Chen M,Fischler B,Hultgren C,Halasz R,Nemeth A,Sällberg M

    更新日期:1999-12-01 00:00:00

  • Use of monoclonal antibodies in an epidemiological marker system: a retrospective study of lung specimens from the 1976 outbreak of Legionnaires disease in Philadelphia by indirect fluorescent-antibody and enzyme-linked immunosorbent assay methods.

    abstract::Autopsy specimens of lung tissues from 15 patients that contracted legionellosis during the 1976 Philadelphia outbreak of Legionnaires disease were examined for the presence of Legionella organisms and soluble antigens by indirect fluorescent-antibody (IFA) testing and by an enzyme-linked immunosorbent assay (ELISA) w...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.21.1.15-19.1985

    authors: Brown SL,Bibb WF,McKinney RM

    更新日期:1985-01-01 00:00:00

  • Seronegative hepatitis C virus infection in a child infected via mother-to-child transmission.

    abstract::Hepatitis C virus (HCV) infection typically leads to antibody response within weeks after primary infection. Here, we describe the case of a child infected with HCV by mother-to-child transmission who remained persistently seronegative despite the presence of high levels of circulating HCV RNA. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00622-12

    authors: Larouche A,Gaëtan G,El-Bilali N,Quesnel-Vallières M,Martin SR,Alvarez F,Shoukry NH,Soudeyns H

    更新日期:2012-07-01 00:00:00

  • Comparative analysis of human cytomegalovirus a-sequence in multiple clinical isolates by using polymerase chain reaction and restriction fragment length polymorphism assays.

    abstract::The human cytomegalovirus (HCMV) a-sequence (a-seq) is located in the joining region between the long (L) and short (S) unique sequences of the virus (L-S junction), and this hypervariable junction has been used to differentiate HCMV strains. The purpose of this study was to investigate whether there are differences a...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.28.12.2602-2607.1990

    authors: Zaia JA,Gallez-Hawkins G,Churchill MA,Morton-Blackshere A,Pande H,Adler SP,Schmidt GM,Forman SJ

    更新日期:1990-12-01 00:00:00

  • Direct examination of unstained smears for the evaluation of sputum specimens.

    abstract::We compared examination of unstained and Gram-stained smears as methods of evaluating sputum quality. Of 100 slides examined, 96 (96%) were graded identically. Each discrepancy between methods was near the cutoff for specimen acceptability. We concluded that examination of unstained smears can provide an accurate, cos...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.24.1.143-144.1986

    authors: Wetterau LM,Zeimis RT,Hollick GE

    更新日期:1986-07-01 00:00:00

  • Subcutaneous hyalohyphomycosis caused by Colletotrichum gloeosporioides.

    abstract::The coelomycete Colletotrichum gloeosporioides was isolated in pure culture from subcutaneous nodules of the left forearm and elbow of a farmer after traumatic injury. To our knowledge, we report the first case involving this fungus as an etiological agent of subcutaneous infection. The in vitro inhibitory activities ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.36.10.3060-3065.1998

    authors: Guarro J,Svidzinski TE,Zaror L,Forjaz MH,Gené J,Fischman O

    更新日期:1998-10-01 00:00:00

  • An Echinococcus multilocularis Antigen B3 Proteoform That Shows Specific Antibody Responses to Active-Stage Alveolar Echinococcosis.

    abstract::Alveolar echinococcosis (AE), caused by the Echinococcus multilocularis metacestode, represents one of the most frequently fatal zoonoses. Early diagnosis significantly reduces morbidity and mortality associated with AE. Diagnosis of AE largely depends on a combination of imaging and serological tests due to its minim...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01362-15

    authors: Ahn CS,Cai H,Kim JG,Han X,Ma X,Bae YA,Yang HJ,Kang I,Wang H,Kong Y

    更新日期:2015-10-01 00:00:00

  • Candida guilliermondii and other species of candida misidentified as Candida famata: assessment by vitek 2, DNA sequencing analysis, and matrix-assisted laser desorption ionization-time of flight mass spectrometry in two global antifungal surveillance pro

    abstract::Candida famata (teleomorph Debaryomyces hansenii) has been described as a medically relevant yeast, and this species has been included in many commercial identification systems that are currently used in clinical laboratories. Among 53 strains collected during the SENTRY and ARTEMIS surveillance programs and previousl...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01686-12

    authors: Castanheira M,Woosley LN,Diekema DJ,Jones RN,Pfaller MA

    更新日期:2013-01-01 00:00:00

  • Detection of cytomegalovirus DNA in human specimens by LightCycler PCR.

    abstract::Detection of human cytomegalovirus (CMV) DNA in clinical specimens is considered a cornerstone in the diagnosis of CMV disease. The aim of this study was to evaluate a newly designed LightCycler-based quantitative CMV PCR. Specimens of human origin (n = 200) were tested using the LightCycler PCR, the quantitative COBA...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.38.11.4006-4009.2000

    authors: Schaade L,Kockelkorn P,Ritter K,Kleines M

    更新日期:2000-11-01 00:00:00

  • Canine strangles case reveals a new host susceptible to infection with Streptococcus equi.

    abstract::We report the first documented case of canine strangles due to infection with Streptococcus equi in a dog with enlarged lymph nodes. Genetic typing, via sequencing of 12 housekeeping genes and the SeM gene, demonstrated the isolate to be a member of a common equine strain type circulating in the United Kingdom. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00571-06

    authors: Ladlow J,Scase T,Waller A

    更新日期:2006-07-01 00:00:00

  • Widespread transfer of resistance genes between bacterial species in an intensive care unit: implications for hospital epidemiology.

    abstract::A transferable plasmid encoding SHV-12 extended-spectrum beta-lactamase, TEM-116, and aminoglycoside resistance was responsible for two sequential clonal outbreaks of Enterobacter cloacae and Acinetobacter baumannii bacteria. A similar plasmid was present among isolates of four different bacterial species. Recognition...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.43.9.4862-4864.2005

    authors: Naiemi NA,Duim B,Savelkoul PH,Spanjaard L,de Jonge E,Bart A,Vandenbroucke-Grauls CM,de Jong MD

    更新日期:2005-09-01 00:00:00

  • Use of the MagNA pure LC automated nucleic acid extraction system followed by real-time reverse transcription-PCR for ultrasensitive quantitation of hepatitis C virus RNA.

    abstract::Hepatitis C virus (HCV) infection is an increasing health problem worldwide. Quantitative assays for HCV viral load are valuable in predicting response to therapy and for following treatment efficacy. Unfortunately, most quantitative tests for HCV RNA are limited by poor sensitivity. We have developed a convenient, hi...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.42.9.4130-4136.2004

    authors: Cook L,Ng KW,Bagabag A,Corey L,Jerome KR

    更新日期:2004-09-01 00:00:00

  • Etiology of diarrhea in young children in Denmark: a case-control study.

    abstract::Infectious gastroenteritis is one of the most common diseases in young children. To clarify the infectious etiology of diarrhea in Danish children less than 5 years of age, we conducted a 2-year prospective case-control study. Stools from 424 children with diarrhea and 870 asymptomatic age-matched controls were examin...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.43.8.3636-3641.2005

    authors: Olesen B,Neimann J,Böttiger B,Ethelberg S,Schiellerup P,Jensen C,Helms M,Scheutz F,Olsen KE,Krogfelt K,Petersen E,Mølbak K,Gerner-Smidt P

    更新日期:2005-08-01 00:00:00

  • Extensive horizontal gene transfer in ureaplasmas from humans questions the utility of serotyping for diagnostic purposes.

    abstract::Ureaplasma parvum and Ureaplasma urealyticum are sexually transmitted, opportunistic pathogens of the human urogenital tract. There are 14 known serovars distributed between the two species. For decades, it has been postulated based upon limited data that virulence is related to serotype specificity. The results were ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00637-11

    authors: Xiao L,Paralanov V,Glass JI,Duffy LB,Robertson JA,Cassell GH,Chen Y,Waites KB

    更新日期:2011-08-01 00:00:00

  • Improved polyacrylamide-based artificial sputum with formalin-fixed tubercle bacilli for training of tuberculosis microscopists.

    abstract::Sputum smear microscopy is an easy, inexpensive, and rapid method for detecting tubercle bacilli when there are more than 10,000 bacilli/ml in the original sputum. Furthermore, because the microscopic method provides not only quantitative, but also qualitative information, such as the shape of bacilli, it has remained...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00370-11

    authors: Yamada H,Mitarai S,Wahyunitisari MR,Mertaniasih NM,Sugamoto T,Chikamatsu K,Aono A,Matsumoto H,Fujiki A

    更新日期:2011-10-01 00:00:00

  • Flow cytometric immunofluorescence assay for detection of antibodies to human immunodeficiency virus type 1 using insoluble precursor forms of recombinant polyproteins as carriers and antigens.

    abstract::A new serological assay, the recombinant flow cytometric immunofluorescence assay (r-FIFA), was developed for the early detection of human immunodeficiency virus type 1 (HIV-1) antibodies by using recombinant insoluble forms of HIV-1 Gag-p45, Gag-gp41 chimeric protein, gp160, Po197 polyprotein as antigens and autologo...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.34.6.1412-1419.1996

    authors: Hu YW,Birch P,Balaskas E,Zeibdawi A,Scalia V,Thériault-Valin SA,Gill P,Aye MT

    更新日期:1996-06-01 00:00:00

  • Design and evaluation of peptide nucleic acid probes for specific identification of Candida albicans.

    abstract::Candida albicans is an important cause of systemic fungal infections, and rapid diagnostics for identifying and differentiating C. albicans from other Candida species are critical for the timely application of appropriate antimicrobial therapy, improved patient outcomes, and pharmaceutical cost savings. In this work, ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02417-14

    authors: Kim HJ,Brehm-Stecher BF

    更新日期:2015-02-01 00:00:00