Seronegative hepatitis C virus infection in a child infected via mother-to-child transmission.

abstract：:The first outbreak caused by vancomycin-resistant enterococci of the VanB phenotype in Poland was analyzed. It occurred in a single ward of a Warsaw hospital which is a specialized center for the treatment of hematological disorders. Between July 1999 and February 2000, 11 patients in the ward were found to be infecte...

journal_title：Journal of clinical microbiology

authors： Kawalec M,Gniadkowski M,Zaleska M,Ozorowski T,Konopka L,Hryniewicz W

更新日期：2001-05-01 00:00:00

abstract：:Pathozyme-Myco G (Myco G), M, A, and TB complex plus (Omega Diagnostics Ltd., Alloa, Scotland) were evaluated for the serological diagnosis of pulmonary tuberculosis (TB) in an Argentinean population. Sera from 58 patients with pulmonary TB, 24 subjects with pulmonary mycobacteriosis or mycoses (pulmonary MM group), a...

journal_title：Journal of clinical microbiology

authors： Imaz MS,Comini MA,Zerbini E,Sequeira MD,Latini O,Claus JD,Singh M

更新日期：2004-02-01 00:00:00

abstract：:Blood cultures (BCs) are the standard method for diagnosis of bloodstream infections (BSIs). However, the average BC contamination rate (CR) in U.S. hospitals is 2.9%, potentially resulting in unnecessary antibiotic use and excessive therapy costs. Several studies have compared various skin antisepsis agents without a...

journal_title：Journal of clinical microbiology

authors： Story-Roller E,Weinstein MP

更新日期：2016-12-01 00:00:00

abstract：:The ability of MicroScan rapid panels (Baxter MicroScan, West Sacramento, Calif.) to detect oxacillin resistance in 92 clinical isolates of Staphylococcus aureus and 103 coagulase-negative staphylococci was evaluated by comparing results with those of MicroScan 24-h MIC panels and, to resolve discrepancies, oxacillin ...

journal_title：Journal of clinical microbiology

authors： Woods GL,LaTemple D,Cruz C

更新日期：1994-04-01 00:00:00

abstract：:Four HIV rapid tests were subjected to field validation in Panama and compared to an enzyme-linked immunosorbent assay/Multispot-based testing algorithm. The sensitivities of Determine, Uni-Gold, SD Bioline, and INSTI were 99.8%. The specificities of Determine, SD-Bioline, and Uni-Gold were 100%, and the specificity o...

journal_title：Journal of clinical microbiology

authors： Juarez SI,Nuñez AE,Aranda MM,Mojica D,Kim AA,Parekh B

更新日期：2016-04-01 00:00:00

abstract：:An outbreak of pseudobacteremia due to Streptococcus pyogenes (group A streptococci [GAS]) and methicillin-susceptible Staphylococcus aureus (MSSA) was traced to the venting procedure for aerobic bottles prior to their loading into the incubator of the BacT/Alert analyzer (Organon Teknika). Bacteria shed by a laborato...

journal_title：Journal of clinical microbiology

authors： Simhon A,Rahav G,Shapiro M,Block C

更新日期：2001-01-01 00:00:00

abstract：:A 13-year-old patient developed severe shock due to administration of a Yersinia enterocolitica-contaminated red blood cell concentrate. Y. enterocolitica (serotype O:9, biotype II) was cultivated from the residual blood in the blood bag and from a stool sample of the blood donor. In the donor's plasma immunoglobulin ...

journal_title：Journal of clinical microbiology

authors： Strobel E,Heesemann J,Mayer G,Peters J,Müller-Weihrich S,Emmerling P

更新日期：2000-07-01 00:00:00

abstract：:A monoclonal antibody specific for the gI glycoprotein of virulent pseudorabies virus was produced and used to affinity purify gI glycoprotein. The purified gI was used in an enzyme-linked immunosorbent assay (ELISA) that identified and differentiated field virus-exposed animals from animals vaccinated with gI-deleted...

journal_title：Journal of clinical microbiology

authors： Mellencamp MW,Pfeiffer NE,Suiter BT,Harness JR,Beckenhauer WH

更新日期：1989-10-01 00:00:00

abstract：:The rate of multidrug-resistant (MDR) and extensively drug-resistant (XDR) tuberculosis (TB) has been steadily increasing in countries of the former USSR. The availability of rapid and reliable methods for the detection of drug resistance to second-line drugs is vital for adequate patient management. We evaluated the ...

journal_title：Journal of clinical microbiology

authors： Ignatyeva O,Kontsevaya I,Kovalyov A,Balabanova Y,Nikolayevskyy V,Toit K,Dragan A,Maxim D,Mironova S,Kummik T,Muntean I,Koshkarova E,Drobniewski F

更新日期：2012-05-01 00:00:00

abstract：:To detect Streptococcus pneumoniae colonization, the nasopharynx is sampled using a swab placed in skim milk-tryptone-glucose-glycerol (STGG) transport medium, and then the swab specimen or STGG medium is cultured or subjected to PCR. We evaluated the effect of swab composition and compared the sensitivities of detect...

journal_title：Journal of clinical microbiology

authors： Rubin LG,Rizvi A,Baer A

更新日期：2008-08-01 00:00:00

abstract：:We first noted the appearance of thymidine-requiring, gram-negative bacilli in clinical specimens 2 years ago. Since then we have seen 10 patients colonized or infected with these organisms. These strains do not grow on Mueller-Hinton media, growth on MacConkey agar is variable, and growth in API 20E (Analytab Product...

journal_title：Journal of clinical microbiology

authors： King CH,Shlaes DM,Dul MJ

更新日期：1983-07-01 00:00:00

abstract：:Mitochondrial cytochrome b (cyt b) genes of 42 strains representing 23 species of the genus Trichosporon were partially sequenced to determine their molecular phylogenetic relationships. Almost half of the 22 strains investigated (from 11 different species) contained introns in their sequences. Analysis of a 396-bp co...

journal_title：Journal of clinical microbiology

authors： Biswas SK,Wang L,Yokoyama K,Nishimura K

更新日期：2005-10-01 00:00:00

abstract：:A Pasteurella multocida species-specific oligonucleotide probe, pmhyb449, targeting 16S rRNA was designed and evaluated by whole-cell hybridization against 22 selected reference strains in animal tissues. It differentiated P. multocida from other bacterial species of the families Pasteurellaceae and Enterobacteriaceae...

journal_title：Journal of clinical microbiology

authors： Mbuthia PG,Christensen H,Boye M,Petersen KM,Bisgaard M,Nyaga PN,Olsen JE

更新日期：2001-07-01 00:00:00

abstract：:We describe the first case of gastritis in a male Australian sea lion (Neophoca cinerea) in which members of the family Helicobacteraceae, particularly the genus Wolinella, were detected. The sea lion exhibited clinical signs of gastrointestinal disease, including abdominal pain, lack of appetite, and lethargy. Examin...

journal_title：Journal of clinical microbiology

authors： Oxley AP,Powell M,McKay DB

更新日期：2004-08-01 00:00:00

abstract：:Routine anaerobic subcultures of macroscopically negative blood culture bottles, performed within 1 day of receipt of the culture and after 5 days of incubation, were evaluated. Anaerobes were recovered from 207 (12.3%) of the total 1,688 positive cultures and, of these, 154 were only detected macroscopically, 11 only...

journal_title：Journal of clinical microbiology

authors： Murray PR,Sondag JE

更新日期：1978-10-01 00:00:00

abstract：:A study of immunofluorescence of yeast in the urine was carried out in 18 patients with funguria in an effort to correlate gamma globulin coating with evidence of tissue invasion or as an indicator for institution of therapy. Positive immunofluorescence of yeast in urine is common but is neither indicative of upper ur...

journal_title：Journal of clinical microbiology

authors： Everett ED,Eickhoff TC,Ehret JM

更新日期：1976-08-01 00:00:00

abstract：:The aim of this study was to develop and evaluate multiplex and nested PCR-reverse line blot (RLB) hybridization assays for detection and serovar identification of Chlamydia trachomatis. Two sets of primers targeting the VD2 region of the omp1 gene and one set targeting the cryptic plasmid were designed for use in mul...

journal_title：Journal of clinical microbiology

authors： Xiong L,Kong F,Zhou H,Gilbert GL

更新日期：2006-04-01 00:00:00

abstract：:Electrophoretic karyotypes of atypical isolates of Candida albicans, e.g., strains that were germ tube negative, failed to express proteinase activity, demonstrated low virulence for mice, formed hyperchlamydoconidia, produced hyperhyphae, or were sucrose negative (including the type strain of Candida stellatoidea), w...

journal_title：Journal of clinical microbiology

authors： Mahrous M,Lott TJ,Meyer SA,Sawant AD,Ahearn DG

更新日期：1990-05-01 00:00:00

abstract：:An enzymatic profile of 20 strains of Pseudomonas maltophilia was undertaken with conventional plate tests, API ZYM, and 4-methylumbelliferyl-conjugated substrates. All strains produced DNase, RNase, arbutinase, esterases and lipases, mucinase, acid and alkaline phosphatases, alkaline pyrophosphate diesterase, phospho...

journal_title：Journal of clinical microbiology

authors： O'Brien M,Davis GH

更新日期：1982-09-01 00:00:00

abstract：:Rapid detection of drug resistance in Mycobacterium tuberculosis is essential for efficient treatment and control of this pathogen. The amplification refractory mutation system (ARMS) was used to detect mutations in the rifampin resistance-determining region of the rpoB gene. A total of 39 rifampin-resistant M. tuberc...

journal_title：Journal of clinical microbiology

authors： Fan XY,Hu ZY,Xu FH,Yan ZQ,Guo SQ,Li ZM

更新日期：2003-03-01 00:00:00

abstract：:We hypothesized that diversion of the first milliliter of venipuncture blood-the initial specimen diversion technique (ISDT)-would eliminate incompletely sterilized fragments of skin from the culture specimen and significantly reduce our blood culture contamination rate (R). We studied our hypothesis prospectively beg...

journal_title：Journal of clinical microbiology

authors： Patton RG,Schmitt T

更新日期：2010-12-01 00:00:00

abstract：:We report a failure of the real-time reverse transcriptase PCR H7 subtyping protocol currently used in national avian influenza surveillance programs. Significant substitutions in primer and probe target sequences were identified, especially in wild bird viruses. The protocol, originally designed for detecting H7 infl...

journal_title：Journal of clinical microbiology

authors： Xing Z,Cardona C,Dao P,Crossley B,Hietala S,Boyce W

更新日期：2008-05-01 00:00:00

abstract：:A new, rapid method for the detection of human immunodeficiency virus type 1 (HIV-1) antibody by time-resolved fluoroimmunoassay (TR-FIA) was developed. In this assay format, microtitration strips were coated with a recombinant fusion protein, and the same protein was labeled with europium and added into the wells sim...

journal_title：Journal of clinical microbiology

authors： Siitari H,Turunen P,Schrimsher J,Nunn M

更新日期：1990-09-01 00:00:00

abstract：:Disseminated disease by Aspergillus granulosus has been reported only once previously in a cardiac transplant recipient. We report a fatal central nervous system infection in a lung transplant recipient. Key features of this species in the section Usti include growth at 37 degrees C and large, randomly spaced aggregat...

journal_title：Journal of clinical microbiology

authors： Sutton DA,Wickes BL,Romanelli AM,Rinaldi MG,Thompson EH,Fothergill AW,Dishop MK,Elidemir O,Mallory GB,Moonnamakal SP,Adesina AM,Schecter MG

更新日期：2009-10-01 00:00:00

abstract：:We assessed neutralizing antibody titers to adenovirus serotype 5 (Ad5) and six rare adenovirus serotypes, serotypes 11, 35, 50, 26, 48, and 49, in pediatric populations in sub-Saharan Africa. We observed a clear age dependence of Ad5-specific neutralizing antibody titers. These data will help to guide the development...

journal_title：Journal of clinical microbiology

authors： Thorner AR,Vogels R,Kaspers J,Weverling GJ,Holterman L,Lemckert AA,Dilraj A,McNally LM,Jeena PM,Jepsen S,Abbink P,Nanda A,Swanson PE,Bates AT,O'Brien KL,Havenga MJ,Goudsmit J,Barouch DH

更新日期：2006-10-01 00:00:00

abstract：:The R/B and Minitek systems for the identification of Enterobacteriaceae were evaluated, and the results were compared with those obtained by conventional methods. Both systems were rapid and allowed correct identification of about 85% of the 294 isolates (23 species) examined. The individual biochemical reactions sho...

journal_title：Journal of clinical microbiology

authors： Shayegani M,Hubbard ME,Hiscott T,McGlynn D

更新日期：1975-06-01 00:00:00

abstract：:Serogrouping of Bacteroides nodosus is based on antigenic differences in fimbriae of the different New Zealand prototype strains. Because of the time needed to isolate and grow pure cultures of B. nodosus and the difficulty in distinguishing between different serogroups because of cross-agglutination, a new DNA-based ...

journal_title：Journal of clinical microbiology

authors： John GH,Carlson JO,Kimberling CV,Ellis RP

更新日期：1990-11-01 00:00:00

abstract：:Outbreaks of food-borne listeriosis have often involved strains of serotype 4b. Examination of multiple isolates from three different outbreaks revealed that ca. 11 to 29% of each epidemic population consisted of strains which were negative with the serotype-specific monoclonal antibody c74.22, lacked galactose from t...

journal_title：Journal of clinical microbiology

authors： Clark EE,Wesley I,Fiedler F,Promadej N,Kathariou S

更新日期：2000-10-01 00:00:00

abstract：:One hundred ninety-three Streptococcus agalactiae isolates of neonatal origin and 146 isolates from adult women were analyzed for macrolide resistance and investigated for clonality. Among erythromycin-resistant isolates, serotype V turned out to be the most frequent. Comparative pulsed-field gel electrophoresis analy...

journal_title：Journal of clinical microbiology

authors： von Both U,Ruess M,Mueller U,Fluegge K,Sander A,Berner R

更新日期：2003-05-01 00:00:00

abstract：:Invasive pulmonary aspergillosis (IPA) is a frequently fatal infection in immunocompromised patients that is difficult to diagnose. Present methods for detection of Aspergillus spp. in bronchoalveolar lavage (BAL) fluid and in tissue vary in sensitivity and specificity. We therefore developed an A. fumigatus-specific ...

journal_title：Journal of clinical microbiology

authors： O'Sullivan CE,Kasai M,Francesconi A,Petraitis V,Petraitiene R,Kelaher AM,Sarafandi AA,Walsh TJ

更新日期：2003-12-01 00:00:00