Detection of resistance to second-line antituberculosis drugs by use of the genotype MTBDRsl assay: a multicenter evaluation and feasibility study.

abstract：:An atypical toxin variant of Clostridium botulinum (strain 657) was isolated from the feces of a 6-week-old female infant whose symptoms and clinical history were consistent with infant botulism. Toxin detected in the feces and the toxin produced by isolates from the feces and from two rectal swabs could be neutralize...

journal_title：Journal of clinical microbiology

authors： Hatheway CL,McCroskey LM,Lombard GL,Dowell VR Jr

更新日期：1981-12-01 00:00:00

abstract：:Morganella ("Proteus") morganii is the only species in the recently proposed genus Morganella, but we suspect there are yet undescribed species in the genus. Two candidates for new species were recently investigated. Nineteen strains isolated from clinical specimens resembled M. morganii but were lysine positive and n...

journal_title：Journal of clinical microbiology

authors： Hickman FW,Framer JJ 3rd,Steigerwalt AG,Brenner DJ

更新日期：1980-07-01 00:00:00

abstract：:Clostridium difficile 027/NAP1/BI is the most common C. difficile strain in the United States. The Xpert C. difficile/Epi assay allows rapid, presumptive identification of C. difficile NAP1. We compared Xpert C. difficile/Epi to multilocus sequence typing for identification of C. difficile NAP1 and found "very good" a...

journal_title：Journal of clinical microbiology

authors： McMillen T,Kamboj M,Babady NE

更新日期：2016-03-01 00:00:00

abstract：:To establish the exact pathogenic role of Mycobacterium abscessus in cystic fibrosis (CF), molecular tests are required for accurate identification. Forty-eight M. abscessus isolates from seven patients with CF were analyzed by sequence analysis for sequence variants within the hsp65 gene and the 16S-23S intergenic se...

journal_title：Journal of clinical microbiology

authors： König B,Tammer I,Sollich V,König W

更新日期：2005-07-01 00:00:00

abstract：:Parasite-specific humoral and cellular immune responses were evaluated in in nine immunosuppressed patients with Strongyloides stercoralis infection. Four patients had the disseminated form of the disease, and five had serious manifestations without evidence of hyperinfection. All patients had high levels of immunoglo...

journal_title：Journal of clinical microbiology

authors： Genta RM,Douce RW,Walzer PD

更新日期：1986-06-01 00:00:00

abstract：:Diagnostic laboratories are under increasing pressure to improve and expand their services. Greater flexibility in sample processing is a critical factor that can improve the time to results while reducing reagent waste, making laboratories more efficient and cost-effective. The introduction of the Abbott mPLUS featur...

journal_title：Journal of clinical microbiology

authors： Lucic D,Jones S,Wiesneth R,Barry C,Webb E,Belova L,Dolan P,Ho S,Abravaya K,Cloherty G

更新日期：2013-12-01 00:00:00

abstract：:Twenty-three young adult rhesus monkeys from China were evaluated for the presence of Helicobacter pylori. Gastric body and antral biopsy samples were tested for H. pylori by PCR analysis, culture, rapid urease testing, and histologic evaluation. Serologic testing to detect H. pylori immunoglobulin G (IgG) antibodies ...

journal_title：Journal of clinical microbiology

authors： Handt LK,Fox JG,Yan LL,Shen Z,Pouch WJ,Ngai D,Motzel SL,Nolan TE,Klein HJ

更新日期：1997-01-01 00:00:00

abstract：:Ninety-eight isolates of Cryptococcus neoformans were collected from 30 patients at the University of Iowa Hospitals and Clinics from December 1987 through December 1994. The susceptibility of each isolate was determined against fluconazole, itraconazole, amphotericin B, and flucytosine. Of the 98 isolates, 53 were re...

journal_title：Journal of clinical microbiology

authors： Klepser ME,Pfaller MA

更新日期：1998-12-01 00:00:00

abstract：:This study determined the group A rotavirus occurrence in pig farms from 7 different cities in São Paulo State, Brazil. Out of 143 samples, 70 tested positive. Sequence analyses of 37 strains indicated that the strains had the G3, G5, G9, and P[6], P[13]/P[22]-like, and P[23] genotypes. ...

journal_title：Journal of clinical microbiology

authors： Tonietti PO,Hora AS,Silva FD,Ruiz VL,Gregori F

更新日期：2013-08-01 00:00:00

abstract：:This study examines the microbiological and epidemiological characteristics of toxigenic and non-toxigenic Corynebacterium isolates submitted to the national reference laboratory in Spain, between 2014 and 2019, in order to describe the current situation and improve our knowledge regarding these emerging pathogens. Ep...

journal_title：Journal of clinical microbiology

authors： Hoefer A,Pampaka D,Herrera-León S,Peiró S,Varona S,López-Perea N,Masa-Calles J,Herrera-León L

更新日期：2020-12-09 00:00:00

abstract：:An enzyme-linked immunosorbent assay was developed for the detection of circulating free and complexed staphylococcal antigens in various clinical categories of staphylococcal infections. Circulating immune complexes were studied by the polyethylene glycol precipitation method. Circulating immune complexes and staphyl...

journal_title：Journal of clinical microbiology

authors： Lentino JR,Rytel MW

更新日期：1982-12-01 00:00:00

abstract：:Detection of residual plasma viremia in antiretroviral therapy (ART)-suppressed HIV-infected individuals is critical for characterizing the latent reservoir and evaluating the impact of cure interventions. Ultracentrifugation-based single-copy assays are sensitive but labor intensive. Fully automated replicate testing...

journal_title：Journal of clinical microbiology

authors： Bakkour S,Deng X,Bacchetti P,Grebe E,Montalvo L,Worlock A,Stone M,Deeks SG,Richman DD,Busch MP

更新日期：2020-11-18 00:00:00

abstract：:We describe a method for the simultaneous identification of up to three Mycoplasma species by the use of contrast-labeled fluorescent antibodies against two species and peroxidase-labeled antibody against a third species. The procedure enabled the rapid identification of colonies of three artificially mixed avian Myco...

journal_title：Journal of clinical microbiology

authors： Bencina D,Bradbury JM

更新日期：1992-02-01 00:00:00

abstract：:Meningococcal disease caused by N. meningitidis serogroup B (MenB) has been endemic in Brazil since 1997. In this study, we determined the prevalence of serosubtypes of MenB isolated in 10 Brazilian states and the Federal District during 1997 and 1998 and investigated the extent of PorA VR sequence variation among the...

journal_title：Journal of clinical microbiology

authors： Sacchi CT,Lemos AP,Popovic T,De Morais JC,Whitney AM,Melles CE,Brondi LM,Monteiro LM,Paiva MV,Solari CA,Mayer LW

更新日期：2001-08-01 00:00:00

abstract：:A mucoid strain of Pseudomonas aeruginosa isolated from a patient with cystic fibrosis and its nonmucoid revertant were grown in a chemically defined alginate-promoting medium under batch and continuous culture conditions. Selection for the mucoid and nonmucoid phenotype was accomplished by varying the levels of air a...

journal_title：Journal of clinical microbiology

authors： Krieg DP,Bass JA,Mattingly SJ

更新日期：1986-12-01 00:00:00

abstract：:Carbapenem-resistant Enterobacter species are emerging nosocomial pathogens. As with most multidrug-resistant Gram-negative pathogens, the polymyxins are often the only therapeutic option. In this study involving clinical isolates of E. cloacae and E. aerogenes, susceptibility testing methods with polymyxin B were ana...

journal_title：Journal of clinical microbiology

authors： Landman D,Salamera J,Quale J

更新日期：2013-12-01 00:00:00

abstract：:In a stress test, the recently introduced RapID CB Plus system (Remel Inc. [formerly Innovative Diagnostic Systems], Norcross, Ga.) was challenged with a diverse set of gram-positive rods comprising 345 strains of coryneform bacteria and 33 strains of Listeria spp. representing a total of 49 different taxa. Overall, w...

journal_title：Journal of clinical microbiology

authors： Funke G,Peters K,Aravena-Roman M

更新日期：1998-09-01 00:00:00

abstract：:The usefulness of pneumococcal urinary antigen tests (UATs) in severe pneumococcal infection relies heavily on the performance in bacteremic patients. Fluorescence technology and automatic reading of test results may improve UAT performance. We evaluated the automatically read Sofia S. pneumoniae FIA for diagnosing pn...

journal_title：Journal of clinical microbiology

authors： Olofsson E,Özenci V,Athlin S

更新日期：2019-07-26 00:00:00

abstract：:A sensitive and reproducible enzyme-linked immunosorbent assay (ELISA) is described for the detection of immunoglobulin M and antibodies with specifity for human cytomegalovirus (CMV) early (CMV-EA) and late (CMV-LA) antigens. The emphasis is on the production of high-quality CMV antigens, CMV-EA and CMV-LA separately...

journal_title：Journal of clinical microbiology

authors： Middeldorp JM,Jongsma J,ter Haar A,Schirm J,The TH

更新日期：1984-10-01 00:00:00

abstract：:Two hundred and twelve Micrococcaceae isolates were obtained from 82 men with nongonococcal urethritis, 24 women with vaginitis, and 54 girls with vulvovaginitis. Identification and biotyping of these strains were carried out by using the simplified scheme of Kloos and Schleifer (W. E. Kloos and K. H. Schleifer, J. Cl...

journal_title：Journal of clinical microbiology

authors： Baldellon C,Mégraud F

更新日期：1985-03-01 00:00:00

abstract：:Ectromelia virus, an orthopoxvirus that can cause extensive morbidity and mortality (mousepox) in colonized mice, has been epizootically responsible for serious disruption of biomedical research since 1930. The lack of a sensitive and specific serological assay for infection with this virus became apparent during outb...

journal_title：Journal of clinical microbiology

authors： Buller RM,Bhatt PN,Wallace GD

更新日期：1983-11-01 00:00:00

abstract：:Human herpesvirus 6 (HHV-6) variant B is frequently identified in peripheral blood, but identification of HHV-6 variant A is relatively rare. We devised a PCR-based method for sensitive, simultaneous detection of both HHV-6 variants. The method was applied to 34 lung tissue specimens that were previously shown to cont...

journal_title：Journal of clinical microbiology

authors： Cone RW,Huang ML,Hackman RC,Corey L

更新日期：1996-04-01 00:00:00

abstract：:A 10-min latex agglutination test kit, Culturette Brand Ten-Minute Group A Strep ID (Marion Scientific, Div. of Marion Laboratories, Inc., Kansas City, Mo.), was assessed for the rapid detection of group A beta-hemolytic streptococcus directly from a throat swab. Four hundred and thirty-five throat swab specimens from...

journal_title：Journal of clinical microbiology

authors： Chang MJ,Mohla C

更新日期：1985-02-01 00:00:00

abstract：:We sought evidence of toxoplasma parasitemia among 37 people with active or dormant Toxoplasma gondii infection or no evidence of infection. DNA was extracted from erythrocyte-free portions of blood samples, and the T. gondii B1 gene was amplified by the polymerase chain reaction. Evidence of T. gondii parasitemia was...

journal_title：Journal of clinical microbiology

authors： Filice GA,Hitt JA,Mitchell CD,Blackstad M,Sorensen SW

更新日期：1993-09-01 00:00:00

abstract：:Panton-Valentine leukocidin (PVL) is a virulence factor of Staphylococcus aureus, which is associated with skin and soft-tissue infections and necrotizing pneumonia. To develop a rapid phenotypic assay, recombinant PVL F component was used to generate monoclonal antibodies by phage display. These antibodies were spott...

journal_title：Journal of clinical microbiology

authors： Monecke S,Müller E,Buechler J,Rejman J,Stieber B,Akpaka PE,Bandt D,Burris R,Coombs G,Hidalgo-Arroyo GA,Hughes P,Kearns A,Abós SM,Pichon B,Skakni L,Söderquist B,Ehricht R

更新日期：2013-02-01 00:00:00

abstract：:We compared a new colony hybridization assay with an established enzyme-linked immunosorbent assay for detection of enterotoxigenic Escherichia coli (ETEC) expressing colonization factor antigen I (CFA/I). The tests were applied to 135 human ETEC strains. Of these isolates, 30 had previously been characterized for CFA...

journal_title：Journal of clinical microbiology

authors： Grewal HM,Sommerfelt H,Gaastra W,Svennerholm AM,Bhan MK,Hamers AM,Kumar R,Wiklund G,Bjorvatn B

更新日期：1990-10-01 00:00:00

abstract：:The HERPCHEK herpes simplex virus (HSV) antigen test (Du Pont, Billerica, Mass.) is a biotinstreptavidin-amplified enzyme-linked immunoassay for the detection of HSV antigen directly in clinical specimens. In a total of 200 mucocutaneous specimens, predominantly lesions from nongenital sites, HERPCHEK had a sensitivit...

journal_title：Journal of clinical microbiology

authors： Wu TC,Zaza S,Callaway J

更新日期：1989-08-01 00:00:00

abstract：:Periodic monitoring of antimicrobial susceptibility of Neisseria gonorrhoeae is essential for early detection of emergence of drug resistance. A total of 343 gonococcal strains isolated from high-risk and general populations in Bangladesh from 1997 to 1999 were studied. The MICs of penicillin, tetracycline, ciprofloxa...

journal_title：Journal of clinical microbiology

authors： Rahman M,Sultan Z,Monira S,Alam A,Nessa K,Islam S,Nahar S,Shama-A-Waris,Alam Khan S,Bogaerts J,Islam N,Albert J

更新日期：2002-06-01 00:00:00

abstract：:Accreditation in Ontario, Canada, requires that licensed clinical laboratories participate in external quality assessment (also known as proficiency testing) and perform competency evaluation of their staff. To assess the extent of ongoing competency assessment practices, the Quality Management Program--Laboratory Ser...

journal_title：Journal of clinical microbiology

authors： Desjardins M,Fleming CA

更新日期：2014-08-01 00:00:00

abstract：:A multiplex method using the SNaPshot technique was developed to screen for six common mycobacterial species: Mycobacteria tuberculosis, M. avium, M. intracellulare, M. chelonae, M. kansasii, and M. gordonae. A total of 468 mycobacterial clinical isolates were subjected to analysis for the presence of the six mycobact...

journal_title：Journal of clinical microbiology

authors： Wang H,Yue J,Han M,Yang J,Zhao Y

更新日期：2010-01-01 00:00:00