当前位置： SCI文献检索 > JOURNAL OF CLINICAL MICROBIOLOGY期刊下所有文献 > Molecular and epidemiological characterisation of toxigenic and non-toxigenic C. diphtheriae , C. belfantii and C. ulcerans isolates identified in Spain from 2014 to 2019.

Molecular and epidemiological characterisation of toxigenic and non-toxigenic C. diphtheriae , C. belfantii and C. ulcerans isolates identified in Spain from 2014 to 2019.

Abstract：

:This study examines the microbiological and epidemiological characteristics of toxigenic and non-toxigenic Corynebacterium isolates submitted to the national reference laboratory in Spain, between 2014 and 2019, in order to describe the current situation and improve our knowledge regarding these emerging pathogens. Epidemiological information was extracted from the Spanish Surveillance System. Microbiological and molecular characterisation was carried out using phenotypic methods, multilocus sequence typing (MLST), whole genome sequencing (WGS) and core genome MLST (cgMLST). Thirty-nine isolates were analysed. Twenty-one were identified as C. diphtheriae (six toxigenic), 14 as C. belfantii and four as C. ulcerans (three toxigenic). One C. diphtheriae isolate was identified as non-toxigenic tox gene-bearing (NTTB). Ages of patients ranged from one to 89 years, with 10% (3/30) of non-toxigenic and 22% (2/9) of toxigenic isolates collected from children less than 15 years. Twenty-five of the patients were males (17/30 in non-toxigenic; 8/9 in toxigenic). MLST identified 28 sequence types (STs) of which seven were described for the first time in Spain. WGS analysis showed that 10 isolates, including three toxigenic isolates, harbored a variety of antibiotic resistance genes in addition to the high prevalence of penicillin resistance phenotypically demonstrated. Phylogenetic analysis revealed one cluster of isolates from family members. Risk information was available for toxigenic isolates (9/39); three patients reported recent travels to endemic countries and three had contact with cats/dogs. One unvaccinated child with respiratory diphtheria had a fatal outcome. Including non-toxigenic Corynebacterium infections in the disease surveillance and using WGS could further improve current surveillance.

journal_name

J Clin Microbiol

journal_title

Journal of clinical microbiology

authors

Hoefer A,Pampaka D,Herrera-León S,Peiró S,Varona S,López-Perea N,Masa-Calles J,Herrera-León L

doi

10.1128/JCM.02410-20

subject

Has Abstract

pub_date

2020-12-09 00:00:00

eissn

0095-1137

issn

1098-660X

pii

JCM.02410-20

pub_type

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Molecular and epidemiological characterisation of toxigenic and non-toxigenic C. diphtheriae , C. belfantii and C. ulcerans isolates identified in Spain from 2014 to 2019.