Intra- and interpatient variability of the hsp65 and 16S-23S intergenic gene region in Mycobacterium abscessus strains from patients with cystic fibrosis.

abstract：:Isolation of Burkholderia pseudomallei currently relies on the use of Ashdown's selective agar (ASA). We designed a new selective agar (Burkholderia pseudomallei selective agar [BPSA]) to improve recovery of the more easily inhibited strains of B. pseudomallei. B. pseudomallei, Burkholderia cepacia, and Pseudomonas ae...

journal_title：Journal of clinical microbiology

authors： Howard K,Inglis TJ

更新日期：2003-07-01 00:00:00

abstract：:In a prospective study, 139 serial blood samples from 15 transplant recipients were assessed for the presence of cytomegalovirus (CMV) by virus isolation (CMV viremia) and by direct staining of CMV antigens (CMV Ag) in blood leukocytes (CMV antigenemia). CMV was isolated from 23 samples, whereas CMV Ag was detected in...

journal_title：Journal of clinical microbiology

authors： van der Bij W,Schirm J,Torensma R,van Son WJ,Tegzess AM,The TH

更新日期：1988-12-01 00:00:00

abstract：:Direct heteroduplex analysis and a universal heteroduplex generator assay were performed to detect rifampin resistance rapidly in Turkish Mycobacterium tuberculosis isolates. Cross-resistance to rifapentine, rifabutin, and rifalazil was investigated. A relationship between specific mutations and resistance patterns, w...

journal_title：Journal of clinical microbiology

authors： Saribaş Z,Kocagöz T,Alp A,Günalp A

更新日期：2003-02-01 00:00:00

abstract：:The indirect immunoperoxidase antibody technique (IPA) has been applied to determine immunoglobulin (Ig)G to humans cytomegalovirus (CMV) antibodies in 114 blood donor sera, four cases of congenital cytomegalic inclusion disease, and four cases of acquired CMV infection. The results have been compared with those obtai...

journal_title：Journal of clinical microbiology

authors： Gerna G,McCloud CJ,Chambers RW

更新日期：1976-03-01 00:00:00

abstract：:Bacillus cereus is a well-known cause of food-borne illness, but infection with this organism is not commonly reported because of its usually mild symptoms. A fatal case due to liver failure after the consumption of pasta salad is described and demonstrates the possible severity of the emetic syndrome. ...

journal_title：Journal of clinical microbiology

authors： Dierick K,Van Coillie E,Swiecicka I,Meyfroidt G,Devlieger H,Meulemans A,Hoedemaekers G,Fourie L,Heyndrickx M,Mahillon J

更新日期：2005-08-01 00:00:00

abstract：:Ewingella americana was isolated from two blood cultures from a 75-year-old male after cholecystectomy. The characteristics of this strain are compared with the reported biochemical characteristics of 44 American strains. Previously described infections and pseudoinfections with E. americana are reviewed. ...

journal_title：Journal of clinical microbiology

authors： Devreese K,Claeys G,Verschraegen G

更新日期：1992-10-01 00:00:00

abstract：:Three molecular assays were evaluated for the direct detection of Mycobacterium tuberculosis complex bacteria in 125 respiratory and 22 nonrespiratory samples. The overall sensitivities obtained were as follows: GenoType MTBDRplus, 97.9%; GenoType Mycobacteria Direct, 93.7%; Gen-Probe Mycobacterium tuberculosis Amplif...

journal_title：Journal of clinical microbiology

authors： Neonakis IK,Gitti Z,Baritaki S,Petinaki E,Baritaki M,Spandidos DA

更新日期：2009-08-01 00:00:00

abstract：:In this report, we present a case of rhino-orbital mucormycosis in a 57-year-old female with poorly controlled diabetes mellitus. The only mold cultured at 25°C, 37°C, and 40°C from a specimen of the nasal crust was identified phenotypically and independently using nuclear ribosomal DNA sequence data as Thamnostylum l...

journal_title：Journal of clinical microbiology

authors： Xess I,Mohapatra S,Shivaprakash MR,Chakrabarti A,Benny GL,O'Donnell K,Padhye AA

更新日期：2012-04-01 00:00:00

abstract：:Restriction endonuclease analysis of virulence plasmid DNA was used to study the epidemiology of Yersinia pseudotuberculosis infections. The origin of Y. pseudotuberculosis could be divided into two focus areas: Eastern Asia and Europe. Wild animals were an important reservoir for the Y. pseudotuberculosis seen in inf...

journal_title：Journal of clinical microbiology

authors： Fukushima H,Gomyoda M,Kaneko S,Tsubokura M,Takeda N,Hongo T,Shubin FN

更新日期：1994-05-01 00:00:00

abstract：:Using PCR-based denaturing gradient gel electrophoresis analyses of oral bacterial samples in 20 mother-child dyads, this study demonstrated a high degree of similarity of bacterial compositions between the mothers and their children; the two may share as much as 94% of their oral bacterial spectra, including cariogen...

journal_title：Journal of clinical microbiology

authors： Li Y,Ismail AI,Ge Y,Tellez M,Sohn W

更新日期：2007-09-01 00:00:00

abstract：:Small round-structured viruses (SRSVs) or Norwalk-like viruses have been implicated as an important causative agent of gastroenteritis outbreaks. We used the relatively newly developed recombinant enzyme immunoassays (EIAs) to determine the seroprevalence of Norwalk virus (NV) and Mexico virus (MxV) in a family-based ...

journal_title：Journal of clinical microbiology

authors： Smit TK,Steele AD,Peenze I,Jiang X,Estes MK

更新日期：1997-09-01 00:00:00

abstract：:Iodophor and alcohol pledgets were compared with the Medi-Flex Prep Kit II for skin disinfection before venipuncture. Of 12,367 blood cultures collected, 6,362 were done with conventional pledgets and 6, 005 were done with Medi-Flex kits. Contamination occurred in 351 of 6,362 blood cultures (5.5%; range, 3.7 to 8.1%)...

journal_title：Journal of clinical microbiology

authors： Wilson ML,Weinstein MP,Mirrett S,Reimer LG,Fernando C,Meredith FT,Reller LB

更新日期：2000-12-01 00:00:00

abstract：:The Lyme disease-associated spirochete, Borrelia burgdorferi, is maintained in enzootic cycles involving Ixodes ticks and small mammals. Previous studies demonstrated that B. burgdorferi expresses outer surface protein A (OspA) but not OspC when residing in the midgut of unfed ticks. However, after ticks feed on blood...

journal_title：Journal of clinical microbiology

authors： Schwan TG,Piesman J

更新日期：2000-01-01 00:00:00

abstract：:Tests for Chlamydia trachomatis and Neisseria gonorrhoeae, which can provide results rapidly to guide therapeutic decision-making, offer patient care advantages over laboratory-based tests that require several days to provide results. We compared results from the Cepheid GeneXpert CT/NG (Xpert) assay to results from t...

journal_title：Journal of clinical microbiology

authors： Gaydos CA,Van Der Pol B,Jett-Goheen M,Barnes M,Quinn N,Clark C,Daniel GE,Dixon PB,Hook EW 3rd,CT\/NG Study Group.

更新日期：2013-06-01 00:00:00

abstract：:Clostridium difficile is the most common cause of health care-associated diarrhea. Accurate and rapid diagnosis is essential to improve patient outcome and prevent disease spread. We compared our two-step diagnostic algorithm, an enzyme immunoassay for glutamate dehydrogenase (GDH) followed by the cytotoxin neutraliza...

journal_title：Journal of clinical microbiology

authors： Babady NE,Stiles J,Ruggiero P,Khosa P,Huang D,Shuptar S,Kamboj M,Kiehn TE

更新日期：2010-12-01 00:00:00

abstract：:The causative agent of Johne's disease is Mycobacterium avium subsp. paratuberculosis. This is a chronic, debilitating gastrointestinal disorder that affects ruminants and is responsible for significant economic loss. The specimen processing method that combines C(18)-carboxypropylbetaine (CB-18) treatment and lytic e...

journal_title：Journal of clinical microbiology

authors： Thornton CG,MacLellan KM,Stabel JR,Carothers C,Whitlock RH,Passen S

更新日期：2002-05-01 00:00:00

abstract：:To study whether pathogenic clusters of Borrelia burgdorferi sensu lato strains occur, we typed 136 isolates, cultured from specimens from patients (n = 49) with various clinical entities and from ticks (n = 83) or dogs (n = 4) from different geographic regions, by randomly amplified polymorphic DNA (RAPD) fingerprint...

journal_title：Journal of clinical microbiology

authors： Wang G,van Dam AP,Spanjaard L,Dankert J

更新日期：1998-03-01 00:00:00

abstract：:This study evaluated the performance of the Rapidec Carba NP test, which was introduced recently into the market for the detection of carbapenemase production in a broad spectrum of β-lactamase-producing Enterobacteriaceae clinical isolates. In total, 252 clinical Enterobacteriaceae isolates that had been genetically ...

journal_title：Journal of clinical microbiology

authors： Hombach M,von Gunten B,Castelberg C,Bloemberg GV

更新日期：2015-12-01 00:00:00

abstract：:We developed a loop-mediated isothermal amplification method able to detect Yersinia pseudotuberculosis strains in 30 min by using six primers designed by targeting the inv gene. This method is more sensitive than PCR and might be a useful tool for detecting and identifying Y. pseudotuberculosis. ...

journal_title：Journal of clinical microbiology

authors： Horisaka T,Fujita K,Iwata T,Nakadai A,Okatani AT,Horikita T,Taniguchi T,Honda E,Yokomizo Y,Hayashidani H

更新日期：2004-11-01 00:00:00

abstract：:The identification of pathogens in patients with bacterial keratitis remains problematic because standard diagnostic tests are negative for 40 to 60% of patients. A cross-sectional study was undertaken to determine if PCR and sequence analysis of 16S ribosomal DNA (rDNA) could be used to detect bacterial pathogens in ...

journal_title：Journal of clinical microbiology

authors： Knox CM,Cevellos V,Dean D

更新日期：1998-12-01 00:00:00

abstract：:Patients with atrophic corpus gastritis and elevated Helicobacter pylori antibody titers but 13C-urea breath test (13C-UBT) and histology results negative for H. pylori were randomized into eradication therapy or follow-up only. Antibody levels decreased significantly in six out of seven patients in the eradication gr...

journal_title：Journal of clinical microbiology

authors： Kokkola A,Rautelin H,Puolakkainen P,Sipponen P,Färkkilä M,Haapiainen R,Kosunen TU

更新日期：1998-06-01 00:00:00

abstract：:CDC group M-5 is a rod-shaped, gram-negative, nonmotile bacterium associated with dog bite wounds. DNA-DNA relatedness and biochemical and growth characteristics were studied for 54 strains from the collection at the Centers for Disease Control and Prevention. One typical M-5 strain, 8142, was further studied by 16S r...

journal_title：Journal of clinical microbiology

authors： Andersen BM,Steigerwalt AG,O'Connor SP,Hollis DG,Weyant RS,Weaver RE,Brenner DJ

更新日期：1993-09-01 00:00:00

abstract：:Fast and accurate identification and typing of pathogens are essential for effective surveillance and outbreak detection. The current routine procedure is based on a variety of techniques, making the procedure laborious, time-consuming, and expensive. With whole-genome sequencing (WGS) becoming cheaper, it has huge po...

journal_title：Journal of clinical microbiology

authors： Joensen KG,Scheutz F,Lund O,Hasman H,Kaas RS,Nielsen EM,Aarestrup FM

更新日期：2014-05-01 00:00:00

abstract：:This study evaluated a PCR method for the rapid detection of clinically significant genotypes of vancomycin-resistant enterococci (VRE) in nosocomial surveillance specimens. Detection of the vanA and vanB genes by multiplex PCR using 657 specimens that showed presumptive growth of VRE on bile esculin azide agar contai...

journal_title：Journal of clinical microbiology

authors： Jayaratne P,Rutherford C

更新日期：1999-06-01 00:00:00

abstract：:The clinical significance of viridans streptococci isolated from the blood cultures of 86 patients was determined. Isolates that were significant or suggestive of infection represented only 21% of the cases. Among 54 isolates for which the species was known, Streptococcus sanguis II was the most common. However, a sig...

journal_title：Journal of clinical microbiology

authors： Swenson FJ,Rubin SJ

更新日期：1982-04-01 00:00:00

abstract：:The polymerase chain reaction was used to detect human herpesvirus 6 (HHV-6) DNA in peripheral blood mononuclear cells from patients with exanthem subitum. Amplified products were detected by agarose gel electrophoresis and dot blot hybridization with a cloned DNA probe. No cross-hybridization with DNAs of five other ...

journal_title：Journal of clinical microbiology

authors： Kondo K,Hayakawa Y,Mori H,Sato S,Kondo T,Takahashi K,Minamishima Y,Takahashi M,Yamanishi K

更新日期：1990-05-01 00:00:00

abstract：:Studies were conducted to compare and evaluate antimicrobial susceptibility test results for enterococci obtained by six national committee disk diffusion procedures. Variations in the incidence of isolates in resistance categories and errors were associated with the use of ciprofloxacin, gentamicin, nitrofurantoin, r...

journal_title：Journal of clinical microbiology

authors： Cotter G,Adley CC

更新日期：2001-10-01 00:00:00

abstract：:The moderately repetitive sequence Ca3 was used to fingerprint strains of Candida albicans isolated from vulvovaginal infections of 10 women and strains isolated from their male partners. The Dendron software package was then used to compare the DNA fingerprints of these strains with those of vaginal commensals from w...

journal_title：Journal of clinical microbiology

authors： Schmid J,Rotman M,Reed B,Pierson CL,Soll DR

更新日期：1993-01-01 00:00:00

abstract：:Gram-negative, anaerobic bacilli are unusual organisms to be isolated in cases of acute septic arthritis. We report the isolation of Fusobacterium necrophorum from joint aspirate in a case of acute septic arthritis, which presented 3 weeks after the drainage of a dental abscess. ...

journal_title：Journal of clinical microbiology

authors： Sonsale PD,Philipson MR,Bowskill J

更新日期：2004-07-01 00:00:00

abstract：:When testing 248 clinical isolates of Neisseria gonorrhoeae, the sensitivity was 100% with GonoGen (Becton Dickinson Microbiology Systems), 99.6% (247 of 248) with GonoGen II (Becton Dickinson), 97.2% (241 of 248) with the MicroTrak direct fluorescent-antibody test (Syva), and 97.6% (242 of 248) with Rapid Fermentatio...

journal_title：Journal of clinical microbiology

authors： Kellogg JA,Orwig LK

更新日期：1995-02-01 00:00:00