Serosubtypes and PorA types of Neisseria meningitidis serogroup B isolated in Brazil during 1997--1998: overview and implications for vaccine development.

abstract：:Many central vascular catheters (CVCs) are removed unnecessarily because current diagnostic methods for CVC-associated infection are unreliable. A quantitative PCR assay using primers and probe targeted to bacterial 16S ribosomal DNA was used to measure the levels of bacterial DNA in blood samples drawn through the CV...

journal_title：Journal of clinical microbiology

authors： Warwick S,Wilks M,Hennessy E,Powell-Tuck J,Small M,Sharp J,Millar MR

更新日期：2004-04-01 00:00:00

abstract：:Streptococcus pneumoniae is an important cause of community-acquired pneumonia. However, in this setting the diagnostic sensitivity of blood cultures is below 30%. Since during such infections changes in the amounts of S. pneumoniae may also occur in the upper respiratory tract, quantification of these bacteria in nas...

journal_title：Journal of clinical microbiology

authors： Greiner O,Day PJ,Bosshard PP,Imeri F,Altwegg M,Nadal D

更新日期：2001-09-01 00:00:00

abstract：:Penicillium marneffei is recognized as one of the most frequently detected opportunistic pathogens of AIDS patients in northern Thailand. We undertook a genomic epidemiology study of 64 P. marneffei isolates collected from immunosuppressed patients by pulsed-field gel electrophoresis (PFGE) with restriction enzyme Not...

journal_title：Journal of clinical microbiology

authors： Trewatcharegon S,Sirisinha S,Romsai A,Eampokalap B,Teanpaisan R,Chaiyaroj SC

更新日期：2001-12-01 00:00:00

abstract：:Freshly isolated clinical strains of Klebsiella were tested for the ability to fix dinitrogen by the acetylene reduction assay. Ability to detect this trait was markedly affected by cultural conditions. When the test was run at 37 degrees C in the presence of yeast extract (50 mg/liter), only 1.6% of the organisms wer...

journal_title：Journal of clinical microbiology

authors： Chambers CA,Silver WS

更新日期：1977-11-01 00:00:00

abstract：:We report on a case of conjunctivitis caused by a nutritionally variant streptococcus in a newborn infant. ...

journal_title：Journal of clinical microbiology

authors： Barrios H,Bump CM

更新日期：1986-02-01 00:00:00

abstract：:Four hundred thirty-two lactose-negative colonies isolated from human feces on stool differential agar media were flooded with one drop of MUCAP Test reagent (Biolife Italiana S.r.I., Milan, Italy) and observed under a Wood lamp for the development of a blue fluorescence over or around the colony. On the basis of manu...

journal_title：Journal of clinical microbiology

authors： Aguirre PM,Cacho JB,Folgueira L,López M,García J,Velasco AC

更新日期：1990-01-01 00:00:00

abstract：:Rapid diagnostic testing reduces the turnaround time for pathogen identification in the clinical microbiology laboratory, but the impact on patient care and hospital costs is a matter of speculation. Patel et al. (J. Clin. Microbiol. 55:60-67, 2017, https://doi.org/10.1128/JCM.01452-16) investigate the impact of matri...

journal_title：Journal of clinical microbiology

authors： McElvania TeKippe E

更新日期：2016-12-28 00:00:00

abstract：:Lower levels of performance of human papillomavirus (HPV) typing assays in studies using formalin-fixed paraffin-embedded (FFPE) tissue compared to those using exfoliated cervical cells have been reported. The interpretation of current studies is limited by bias in inclusion criteria, sample matching, and methods of c...

journal_title：Journal of clinical microbiology

authors： Dal Bello B,Spinillo A,Alberizzi P,Cesari S,Gardella B,Silini EM

更新日期：2009-07-01 00:00:00

abstract：:Noroviruses (NVs) are important human pathogens that cause acute gastroenteritis. Genetically related animal enteric NVs have also been described, but there is no evidence of interspecies transmission of NVs. In this study we characterized antibody prevalence among domestic pigs by using recombinant capsid antigens of...

journal_title：Journal of clinical microbiology

authors： Farkas T,Nakajima S,Sugieda M,Deng X,Zhong W,Jiang X

更新日期：2005-02-01 00:00:00

abstract：:Mycobacterium abscessus, which consists of the two subspecies M. abscessus subspecies abscessus and M. abscessus subspecies bolletii, can produce rough or smooth colony morphologies. Here we analyzed 50 M. abscessus isolates cultured from the respiratory specimens of 34 patients, 28 (82%) of whom had cystic fibrosis (...

journal_title：Journal of clinical microbiology

authors： Rüger K,Hampel A,Billig S,Rücker N,Suerbaum S,Bange FC

更新日期：2014-01-01 00:00:00

abstract：:Using 920 sera, we compared the specificity and reproducibility of the hemagglutination treponemal test for syphilis with those of the fluorescent treponemal antibody-absorption test and the microhemagglutination assay for Treponema pallidum antibodies; we found all three tests to be comparable. However, the hemagglut...

journal_title：Journal of clinical microbiology

authors： Larsen SA,Hambie EA,Pettit DE,Perryman MW,Kraus SJ

更新日期：1981-10-01 00:00:00

abstract：:Tests for Chlamydia trachomatis and Neisseria gonorrhoeae, which can provide results rapidly to guide therapeutic decision-making, offer patient care advantages over laboratory-based tests that require several days to provide results. We compared results from the Cepheid GeneXpert CT/NG (Xpert) assay to results from t...

journal_title：Journal of clinical microbiology

authors： Gaydos CA,Van Der Pol B,Jett-Goheen M,Barnes M,Quinn N,Clark C,Daniel GE,Dixon PB,Hook EW 3rd,CT\/NG Study Group.

更新日期：2013-06-01 00:00:00

abstract：:Phenotypic, genotypic, and toxin gene analyses have not yet been done all in one for the Nigerian Staphylococcus aureus population. This study provides a comprehensive overview of the molecular epidemiology and genetic diversity of S. aureus strains at the largest university clinic in Ibadan, Nigeria. From 1,300 patie...

journal_title：Journal of clinical microbiology

authors： Ghebremedhin B,Olugbosi MO,Raji AM,Layer F,Bakare RA,König B,König W

更新日期：2009-09-01 00:00:00

abstract：:The QuadFERM+ system (Analytab Products, Plainview, N.Y.), a 2-h carbohydrate degradation method for the identification of Neisseria spp., was evaluated along with a rapid DNase test for confirmation of Branhamella catarrhalis. QuadFERM+ identified 100% of 82 N. gonorrhoeae and 96% of 54 N. meningitidis strains. The t...

journal_title：Journal of clinical microbiology

authors： Janda WM,Zigler KL,Bradna JJ

更新日期：1987-02-01 00:00:00

abstract：:spa typing is a method used in molecular typing of methicillin-resistant Staphylococcus aureus (MRSA). Due to the lack of an amplification product in one of 55 MRSA cases, a new PCR setup, which allows an indicative description of a previously unknown type, was performed. ...

journal_title：Journal of clinical microbiology

authors： Reischauer M,Bernkopf M,Webersinke C

更新日期：2010-02-01 00:00:00

abstract：:The diagnosis of syphilis is challenging and often relies on serologic tests to detect treponemal or nontreponemal antibodies. Recently, the Centers for Disease Control and Prevention and the Association of Public Health Laboratories proposed an update to the syphilis serology testing algorithm, in which serum samples...

journal_title：Journal of clinical microbiology

authors： Binnicker MJ,Jespersen DJ,Rollins LO

更新日期：2011-04-01 00:00:00

abstract：:Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most important causes of hospital infections worldwide. High-level resistance to methicillin is caused by the mecA gene, which encodes an alternative penicillin-binding protein, PBP 2a. To determine the clonal relationships between methicillin-susceptibl...

journal_title：Journal of clinical microbiology

authors： Wielders CL,Fluit AC,Brisse S,Verhoef J,Schmitz FJ

更新日期：2002-11-01 00:00:00

abstract：:Sputum obtained from patients with cystic fibrosis (CF) is highly viscous and often heterogeneous in bacterial distribution. Adding dithiothreitol (DTT) is the standard method for liquefaction prior to processing sputum for molecular detection assays. To determine if DTT treatment homogenizes the bacterial distributio...

journal_title：Journal of clinical microbiology

authors： Stokell JR,Khan A,Steck TR

更新日期：2014-07-01 00:00:00

abstract：:Sera from patients with systemic infections caused by the opportunistic fungus Trichosporon beigelii have been shown to cross-react with anticryptococcal antibodies. We quantitatively compared the amounts of antigen produced and examined the expression of O-acetyl epitopes from 35 strains of T. beigelii isolated from ...

journal_title：Journal of clinical microbiology

authors： Lyman CA,Devi SJ,Nathanson J,Frasch CE,Pizzo PA,Walsh TJ

更新日期：1995-01-01 00:00:00

abstract：:CHROMagar Yersinia (CAY) is a new chromogenic medium for the presumptive detection of virulent Yersinia enterocolitica in stools. Based on a comparative analysis of 1,494 consecutive stools from hospitalized patients, CAY was found to be just as sensitive as the reference medium (cefsulodin-irgasan-novobiocin agar) bu...

journal_title：Journal of clinical microbiology

authors： Renaud N,Lecci L,Courcol RJ,Simonet M,Gaillot O

更新日期：2013-04-01 00:00:00

abstract：:The mold Aureobasidium pullulans was isolated on several nutrient media from a splenic abscess in a patient with disseminated lymphoma. Examination of stained smears and paraffin sections revealed fungal structures characteristic of this organism. This is the first reported association of A. pullulans with an opportun...

journal_title：Journal of clinical microbiology

authors： Salkin IF,Martinez JA,Kemna ME

更新日期：1986-05-01 00:00:00

abstract：:Molecular methodologies were used to identify 28 Achromobacter spp. from patients with cystic fibrosis (CF). Multilocus sequence typing (MLST) identified 17 Achromobacter xylosoxidans isolates (all bla(OXA-114) positive), nine Achromobacter ruhlandii isolates (all bla(OXA-114) positive), one Achromobacter dolens isola...

journal_title：Journal of clinical microbiology

authors： Rodrigues ER,Ferreira AG,Leão RS,Leite CC,Carvalho-Assef AP,Albano RM,Marques EA

更新日期：2015-12-01 00:00:00

abstract：:Multidrug-resistant tuberculosis is an increasing health problem worldwide, especially in developing countries. The PCR-UHG-Rif assay, which detects mutations within the rpoB gene associated with rifampin resistance, was evaluated for its ability and reliability to detect and identify drug-resistant Mycobacterium tube...

journal_title：Journal of clinical microbiology

authors： Mayta H,Gilman RH,Arenas F,Valencia T,Caviedes L,Montenegro SH,Ticona E,Ortiz J,Chumpitaz R,Evans CA,Williams DL,Tuberculosis Working Group in Perú.

更新日期：2003-12-01 00:00:00

abstract：:Rapid detection of drug-resistant bacteria in clinical samples plays an instrumental role in patients' infection management and in implementing effective infection control policies. In the study described in this report, we validated a multiplex TaqMan real-time quantitative PCR (qPCR) assay for the detection of bla(K...

journal_title：Journal of clinical microbiology

authors： Hindiyeh M,Smollan G,Grossman Z,Ram D,Robinov J,Belausov N,Ben-David D,Tal I,Davidson Y,Shamiss A,Mendelson E,Keller N

更新日期：2011-07-01 00:00:00

abstract：:In a clinical trial, Strep-A-Chek (a 10-min chromogenic test) was compared with the bacitracin disk susceptibility test for accuracy and turnaround time in the presumptive identification of Streptococcus pyogenes. Among 461 isolates of beta-hemolytic streptococci (344 throat isolates and 117 isolates from other sites)...

journal_title：Journal of clinical microbiology

authors： Yajko DM,Lawrence J,Nassos P,Young J,Hadley WK

更新日期：1986-09-01 00:00:00

abstract：:In a multicenter study, the consistency of international units expressed by five commercially available rubella virus immunoglobulin G kits was evaluated. The linearity and within-run and between-run precision were determined for each kit. All kits demonstrated good linearity and had within-run and between-run precisi...

journal_title：Journal of clinical microbiology

authors： Dimech W,Bettoli A,Eckert D,Francis B,Hamblin J,Kerr T,Ryan C,Skurrie I

更新日期：1992-03-01 00:00:00

abstract：:While Corynebacterium ulcerans can mimic classical diphtheria, extrapharyngeal infections are extremely rare. Sequencing of the diphtheria toxin (DT)-encoding tox gene of two C. ulcerans isolates from extrapharyngeal infections revealed differences from C. diphtheriae DT sequences, mainly in the translocation and rece...

journal_title：Journal of clinical microbiology

authors： Sing A,Hogardt M,Bierschenk S,Heesemann J

更新日期：2003-10-01 00:00:00

abstract：:Urinary tract infections caused by staphylococci are usually attributed to Staphylococcus epidermidis or S. saprophyticus. The case study reported here describes a persistent urinary tract infection caused by S. haemolyticus in a 38-year-old male whose infection was ultimately resolved through the use of the antibioti...

journal_title：Journal of clinical microbiology

authors： Gunn BA,Davis CE Jr

更新日期：1988-05-01 00:00:00

abstract：:The frequency with which clue cells could be detected in Gram-stained vaginal smears and/or cervical Papanicolaou (Pap) smears was compared with the frequency of Corynebacterium vaginale (Haemophilus vaginalis) isolation in a group of 236 female patients, of whom 221 had vaginitis. Vaginal clue cells were found most o...

journal_title：Journal of clinical microbiology

authors： Smith RF,Rodgers HA,Hines PA,Ray RM

更新日期：1977-03-01 00:00:00

abstract：:We examined mutations in the dihydropteroate synthase (DHPS) genes of Pneumocystis carinii f. sp. hominis (P. carinii) strains isolated from 24 patients with P. carinii pneumonia (PCP) in Japan. DHPS mutations were identified at amino acid positions 55 and/or 57 in isolates from 6 (25.0%) of 24 patients. The underlyin...

journal_title：Journal of clinical microbiology

authors： Takahashi T,Hosoya N,Endo T,Nakamura T,Sakashita H,Kimura K,Ohnishi K,Nakamura Y,Iwamoto A

更新日期：2000-09-01 00:00:00