Treponema-specific tests for serodiagnosis of syphilis: comparative evaluation of seven assays.

abstract：:Suspected outbreaks caused by pseudomonas aeruginosa in 19 hospitals and two motels were studied. On the basis of epidemiological analysis, serological typing, and antibiotic resistance patterns, 17 were classified as single-strain outbreaks. Six were classified as common-source outbreaks: of these, three were caused ...

journal_title：Journal of clinical microbiology

authors： Farmer JJ 3rd,Weinstein RA,Zierdt CH,Brokopp CD

更新日期：1982-08-01 00:00:00

abstract：:Well-defined Legionella pneumophila strains were analyzed by amplification of variable genomic regions with arbitrary and repeat sequence primers. Clinical and environmental outbreak-related isolates showed closely related amplicon patterns. Eleven strains of unrelated origins displayed 10 distinct patterns. Fingerpri...

journal_title：Journal of clinical microbiology

authors： van Belkum A,Struelens M,Quint W

更新日期：1993-08-01 00:00:00

abstract：:To simplify the serotyping of Streptococcus pneumoniae, a dot bot assay has been developed and compared with the standard quellung reaction in 1,082 isolates. The technique has been demonstrated to be sensitive, specific, easy to perform, and inexpensive. The dot blot assay could be useful when large numbers of pneumo...

journal_title：Journal of clinical microbiology

authors： Fenoll A,Jado I,Vicioso D,Casal J

更新日期：1997-03-01 00:00:00

abstract：:A multicenter cross-sectional survey was conducted comparing a commercially available chlamydial optical immunoassay (OIA) to the chlamydial ligase chain reaction (LCR). Endocervical samples from 415 outpatients visiting clinics from three hospitals in South Kalimantan, Indonesia, were evaluated. Relative to the LCR, ...

journal_title：Journal of clinical microbiology

authors： Widjaja S,Cohen S,Brady WE,O'reilly K,Susanto,Wibowo A,Cahyono,Graham RR,Porter KR

更新日期：1999-12-01 00:00:00

abstract：:Quantitative HIV RNA viral load (QVL) assays (Roche Diagnostics) were sensitive and specific when used to diagnose HIV infection in (i) HIV-exposed infants (sensitivity of 100% [63.1 to 100%] and specificity of 100% [97.9 to 100%]) and (ii) suspected acute HIV infection patients with a negative/indeterminate Western b...

journal_title：Journal of clinical microbiology

authors： Lee BE,Plitt SS,Jayaraman GC,Chui L,Singh AE,Preiksaitis JK

更新日期：2012-02-01 00:00:00

abstract：:Identification of clinical isolates of Nocardia to the species level is important for defining the spectrum of disease produced by each species and for predicting antimicrobial susceptibility. We evaluated the usefulness of PCR amplification of a portion of the Nocardia 16S rRNA gene and subsequent restriction endonuc...

journal_title：Journal of clinical microbiology

authors： Conville PS,Fischer SH,Cartwright CP,Witebsky FG

更新日期：2000-01-01 00:00:00

abstract：:Nineteen multidrug-resistant (MDR) Mycobacterium complex strains isolated in a nosocomial outbreak were characterized at the molecular level. The strains were microbiologically characterized as Mycobacterium bovis. The mpt40 sequence was not present in chromosomal DNA from these strains, supporting the fact that they ...

journal_title：Journal of clinical microbiology

authors： Blázquez J,Espinosa de Los Monteros LE,Samper S,Martín C,Guerrero A,Cobo J,Van Embden J,Baquero F,Gómez-Mampaso E

更新日期：1997-06-01 00:00:00

abstract：:Due to a highly homogeneous genetic composition, the subtyping of Salmonella enterica serovar Enteritidis strains to an epidemiologically relevant level remains intangible for pulsed-field gel electrophoresis (PFGE). We reported previously on a highly discriminatory PFGE-based subtyping scheme for S. enterica serovar ...

journal_title：Journal of clinical microbiology

authors： Zheng J,Keys CE,Zhao S,Ahmed R,Meng J,Brown EW

更新日期：2011-01-01 00:00:00

abstract：:The increasing risk of Rift Valley fever virus (RVFV) infection as a global veterinary and public health threat demands the development of safe and accurate diagnostic tests. The aim of this study was to assess the suitability of a baculovirus expression system to produce recombinant RVFV nucleoprotein (N) for use as ...

journal_title：Journal of clinical microbiology

authors： Faburay B,Wilson WC,Secka A,Drolet B,McVey DS,Richt JA

更新日期：2019-09-24 00:00:00

abstract：:The effectiveness of antiretroviral therapy may be limited by the development of human immunodeficiency virus type 1 (HIV-1) resistance. Monitoring for resistance will perhaps allow changes in therapy prior to deterioration in the patient's clinical or immunologic status. Our objective was to develop a rapid, specific...

journal_title：Journal of clinical microbiology

authors： Frenkel LM,Wagner LE 2nd,Atwood SM,Cummins TJ,Dewhurst S

更新日期：1995-02-01 00:00:00

abstract：:A broad range of viral and bacterial pathogens can cause acute respiratory tract infection. For rapid detection of a broad respiratory pathogen spectrum, multiplex real-time PCR is ideal. This study evaluated the performance of the new Luminex NxTAG Respiratory Pathogen Panel (NxTAG-RPP) in comparison with the BioFire...

journal_title：Journal of clinical microbiology

authors： Chen JHK,Lam HY,Yip CCY,Wong SCY,Chan JFW,Ma ESK,Cheng VCC,Tang BSF,Yuen KY

更新日期：2016-07-01 00:00:00

abstract：:We describe two rapid, simple, and reliable procedures for routine purification of hepatitis B virus (HBV) DNA from serum. HBV DNA could be purified from 24 serum samples in 1.5 to 2 h and was recovered in the initial reaction vessel. Both procedures have in common that HBV DNA is complexed with silica particles in th...

journal_title：Journal of clinical microbiology

authors： Boom R,Sol CJ,Heijtink R,Wertheim-van Dillen PM,van der Noordaa J

更新日期：1991-09-01 00:00:00

abstract：:Since January 1988, Staphylococcus aureus strains with a high level of quinolone resistance have been isolated at 17 hospitals and 15 nursing homes in New York City. The majority of these strains were methicillin resistant. The bacteriophage types and susceptibility to other antibiotics were similar to those of quinol...

journal_title：Journal of clinical microbiology

authors： Schaefler S

更新日期：1989-02-01 00:00:00

abstract：:From November 2006 to April 2007, nine nonrepetitive isolates of Klebsiella pneumoniae with reduced susceptibility or resistance to carbapenems were recovered from clinical specimens from separate patients hospitalized in a tertiary care hospital. The imipenem-EDTA synergy test was positive for all isolates. PCR, sequ...

journal_title：Journal of clinical microbiology

authors： Tokatlidou D,Tsivitanidou M,Pournaras S,Ikonomidis A,Tsakris A,Sofianou D

更新日期：2008-03-01 00:00:00

abstract：:About 2.5 years after the first isolation of the VanA phenotype of vancomycin-resistant Enterococcus faecium (VREM) in Poland, the first VREM strains with the VanB phenotype have emerged independently in two different Warsaw hospitals. In one of these the VREM strain was selected during the long-term antimicrobial tre...

journal_title：Journal of clinical microbiology

authors： Kawalec M,Gniadkowski M,Zielińska U,Kłos W,Hryniewicz W

更新日期：2001-02-01 00:00:00

abstract：:An indirect enzyme-linked immunosorbent assay was used for the screening of horse sera from The Gambia for antibodies against African horse sickness virus (AHSV). The AHSV antigen used for coating was semipurified according to the method of Manning and Chen (Curr. Microbiol. 4:381, 1980); control mock-infected Vero ce...

journal_title：Journal of clinical microbiology

authors： Staeuber N,Fye B,Zinsstag J,McCullough KC

更新日期：1993-08-01 00:00:00

abstract：:Detection of human papillomavirus (HPV) E6/E7 oncogene expression may be more predictive of cervical cancer risk than testing for HPV DNA. The Aptima HPV test (Gen-Probe) detects E6/E7 mRNA of 14 oncogenic types. Its clinical performance was compared with that of the Hybrid Capture 2 DNA test (HC2; Qiagen) in women re...

journal_title：Journal of clinical microbiology

authors： Ratnam S,Coutlee F,Fontaine D,Bentley J,Escott N,Ghatage P,Gadag V,Holloway G,Bartellas E,Kum N,Giede C,Lear A

更新日期：2011-02-01 00:00:00

abstract：:Fifteen strains of an anaerobic, catalase-negative, gram-positive diphtheroid-shaped bacterium recovered from human sources were characterized by phenotypic and molecular chemical and molecular genetic methods. The unidentified bacterium showed some resemblance to Actinomyces species and related taxa, but biochemical ...

journal_title：Journal of clinical microbiology

authors： Hall V,Collins MD,Lawson PA,Hutson RA,Falsen E,Inganas E,Duerden B

更新日期：2003-02-01 00:00:00

abstract：:In a survey of bloodstream infection (BSI) isolates across the continental United States, 162 Candida albicans isolates were fingerprinted with the species-specific probe Ca3 and the patterns were analyzed for relatedness with a computer-assisted system. The results demonstrate that particular BSI strains are more hig...

journal_title：Journal of clinical microbiology

authors： Pfaller MA,Lockhart SR,Pujol C,Swails-Wenger JA,Messer SA,Edmond MB,Jones RN,Wenzel RP,Soll DR

更新日期：1998-06-01 00:00:00

abstract：:We present a duplex, real-time PCR assay for detection of Klebsiella pneumoniae carbapenemase (blaKPC) and New Delhi metallo-β-lactamase (blaNDM) genes. Accuracy was assessed with 158 Gram-negative bacillary isolates, including 134 carbapenemase producers. The assay had 100% sensitivity and specificity compared with r...

journal_title：Journal of clinical microbiology

authors： Cunningham SA,Noorie T,Meunier D,Woodford N,Patel R

更新日期：2013-04-01 00:00:00

abstract：:Routine bacterial cultures of corneal scrapings from seven cats with either ulcerative feline keratitis, keratomalacia, or both yielded colonies which were identified by 16S rRNA gene sequencing as Mycoplasma felis (six cases) and Mycoplasma gateae (one case). Identification of the pathogens allowed the use of less em...

journal_title：Journal of clinical microbiology

authors： Gray LD,Ketring KL,Tang YW

更新日期：2005-07-01 00:00:00

abstract：:The Echinococcus Western Blot IgG (LDBIO Diagnostics, Lyon, France), using a whole larval antigen from Echinococcus multilocularis, was evaluated for serodiagnosis and differentiation between two human parasitic infections of worldwide importance: cystic echinococcosis, due to Echinococcus granulosus, and alveolar ech...

journal_title：Journal of clinical microbiology

authors： Liance M,Janin V,Bresson-Hadni S,Vuitton DA,Houin R,Piarroux R

更新日期：2000-10-01 00:00:00

abstract：:Sheep and guinea pigs were immunized with cellular and extracellular antigen from Legionella pneumophila bacteria. After immunization, the animals developed immunoglobulin G titers against the immunizing agent. The same sera were also tested in a Mycoplasma pneumoniae complement fixation test. All the preimmunization ...

journal_title：Journal of clinical microbiology

authors： Berdal BP,Eng J

更新日期：1982-11-01 00:00:00

abstract：:The Aspergillus galactomannan test was performed on cerebrospinal fluid and serum samples from 5 patients with probable cerebral aspergillosis and from 16 control patients. Cerebrospinal fluid galactomannan levels were significantly higher in aspergillosis patients, and most galactomannan was produced intrathecally. C...

journal_title：Journal of clinical microbiology

authors： Viscoli C,Machetti M,Gazzola P,De Maria A,Paola D,Van Lint MT,Gualandi F,Truini M,Bacigalupo A

更新日期：2002-04-01 00:00:00

abstract：:Thirteen representative pathogenic bacterial species were used to create septicemia in rabbits, by injecting 10(6) colony-forming units into the marginal ear vein. At a selected time, usually 30 to 60 min after injection, heart blood was drawn into heparin and dispensed in 5.0-,0.5-, and 0.1-ml volumes into duplicate ...

journal_title：Journal of clinical microbiology

authors： Zierdt CH,Peterson DL,Swan JC,MacLowry JD

更新日期：1982-01-01 00:00:00

abstract：:Many laboratories are unable to perform colistin susceptibility testing. Diffusion-based antimicrobial susceptibility testing methods are not recommended, and not all laboratories have the capacity to perform broth microdilution (BMD). Using a multistep tiered approach, we investigated whether the adapted use of the M...

journal_title：Journal of clinical microbiology

authors： Lutgring JD,Kim A,Campbell D,Karlsson M,Brown AC,Burd EM

更新日期：2019-04-26 00:00:00

abstract：:Three new Salmonella serotypes belonging to Kauffmann's subgenus I (F. Kauffmann, The Bacteriology of Enterobacteriaceae, 1966) were identified. These serotypes were Salmonella brazos 6,14,18:a:e,n,z15, Salmonella midway 6,14,24:d:1,7, and Salmonella balboa 48a, 48b:z41:monophasic. ...

journal_title：Journal of clinical microbiology

authors： Sutch KE,Blackburn BO

更新日期：1980-06-01 00:00:00

abstract：:We report the first documented case of endocarditis in a man infected with Bartonella alsatica, which causes bacteremia in healthy wild rabbits. B. alsatica was identified by serology and culture and by PCR of an aortic valve specimen. B. alsatica should be added to the list of zoonotic agents of blood culture-negativ...

journal_title：Journal of clinical microbiology

authors： Raoult D,Roblot F,Rolain JM,Besnier JM,Loulergue J,Bastides F,Choutet P

更新日期：2006-01-01 00:00:00

abstract：:In this study we describe a multiplex PCR assay for the detection of nine clinically relevant antibiotic resistance genes of Staphylococcus aureus. Conditions were optimized to amplify fragments of mecA (encoding methicillin resistance), aacA-aphD (aminoglycoside resistance), tetK, tetM (tetracycline resistance), erm(...

journal_title：Journal of clinical microbiology

authors： Strommenger B,Kettlitz C,Werner G,Witte W

更新日期：2003-09-01 00:00:00

abstract：:In 1998, an outbreak of systemic infections caused by Bacillus cereus occurred in the Neonatal Intensive Care Unit of the University Hospital Vrije Universiteit, Amsterdam, The Netherlands. Three neonates developed sepsis with positive blood cultures. One neonate died, and the other two neonates recovered. An environm...

journal_title：Journal of clinical microbiology

authors： Van Der Zwet WC,Parlevliet GA,Savelkoul PH,Stoof J,Kaiser AM,Van Furth AM,Vandenbroucke-Grauls CM

更新日期：2000-11-01 00:00:00