Methicillin-resistant strains of Staphylococcus aureus resistant to quinolones.

abstract：:The semen plasma virus load is measured to ensure the safety of sperm processing during medically assisted procreation (MAP) for couples with a human immunodeficiency virus type 1 (HIV-1)-infected man. A practical, automated protocol using the COBAS Ampliprep CAP/CTM kit in the COBAS TaqMan96 system was developed to m...

journal_title：Journal of clinical microbiology

authors： Pasquier C,Sauné K,Raymond S,Moinard N,Daudin M,Bujan L,Izopet J

更新日期：2009-09-01 00:00:00

abstract：:The usefulness of pneumococcal urinary antigen tests (UATs) in severe pneumococcal infection relies heavily on the performance in bacteremic patients. Fluorescence technology and automatic reading of test results may improve UAT performance. We evaluated the automatically read Sofia S. pneumoniae FIA for diagnosing pn...

journal_title：Journal of clinical microbiology

authors： Olofsson E,Özenci V,Athlin S

更新日期：2019-07-26 00:00:00

abstract：:Accurate identification of mycobacterial species and subspecies is essential to evaluate their significance and to perform epidemiological studies. The subspecies of Mycobacterium avium have different attributes but coincide in their zoonotic potential. Our knowledge about M. avium subsp. silvaticum is limited, since ...

journal_title：Journal of clinical microbiology

authors： Rónai Z,Csivincsik Á,Dán Á

更新日期：2015-05-01 00:00:00

abstract：:A point prevalence culture survey was carried out to investigate the prevalence of fecal carriage of vancomycin-resistant enterococci (VRE) among patients admitted to an 800-bed general hospital where no VRE had been isolated previously. Twenty-two of 636 patients (3.5%) were found to be VRE carriers. Eighteen strains...

journal_title：Journal of clinical microbiology

authors： Gordts B,Van Landuyt H,Ieven M,Vandamme P,Goossens H

更新日期：1995-11-01 00:00:00

abstract：:Growth on different media and the influence of culture conditions were studied on 19 recently isolated strains of Haemophilus ducreyi, none of which had more than four passages on artificial media. The results were compared with 10 laboratory strains, which had an unknown number of passages in vitro. For all strains, ...

journal_title：Journal of clinical microbiology

authors： Sturm AW,Zanen HC

更新日期：1984-05-01 00:00:00

abstract：:Mycobacterium tilburgii is a nonculturable nontuberculous mycobacterium identifiable only by molecular methods. We report a case of disseminated M. tilburgii infection illustrating the importance of 16S rRNA gene sequencing to determine the responsible mycobacterial pathogen and the difficulties in tailoring antimycob...

journal_title：Journal of clinical microbiology

authors： Temmerman S,Vandekerckhove L,Sermijn E,Vogelaers D,Claeys G,Vaneechoutte M,Cools P,Callens S

更新日期：2014-05-01 00:00:00

abstract：:Bacteriological culture and real-time PCR (RT-PCR) were used to detect Campylobacter jejuni in fecal samples from a French cohort of 237 patients with Guillain-Barré syndrome (GBS). We provide evidence that diverse serotypes and genotypes of C. jejuni are a major trigger of GBS in France. ...

journal_title：Journal of clinical microbiology

authors： Sivadon-Tardy V,Orlikowski D,Porcher R,Ronco E,Caudie C,Roussi J,Fauchère JL,Mégraud F,Tabor H,Sharshar T,Annane D,Raphaël JC,Gaillard JL

更新日期：2010-06-01 00:00:00

abstract：:Invasive meningococcal disease is mainly caused by Neisseria meningitidis serogroups A, B, C, X, W, and Y. The serogroup is typically determined by slide agglutination serogrouping (SASG) and real-time PCR (RT-PCR). We describe a whole-genome sequencing (WGS)-based method to characterize the capsule polysaccharide syn...

journal_title：Journal of clinical microbiology

authors： Marjuki H,Topaz N,Rodriguez-Rivera LD,Ramos E,Potts CC,Chen A,Retchless AC,Doho GH,Wang X

更新日期：2019-02-27 00:00:00

abstract：:Three molecular assays were evaluated for the direct detection of Mycobacterium tuberculosis complex bacteria in 125 respiratory and 22 nonrespiratory samples. The overall sensitivities obtained were as follows: GenoType MTBDRplus, 97.9%; GenoType Mycobacteria Direct, 93.7%; Gen-Probe Mycobacterium tuberculosis Amplif...

journal_title：Journal of clinical microbiology

authors： Neonakis IK,Gitti Z,Baritaki S,Petinaki E,Baritaki M,Spandidos DA

更新日期：2009-08-01 00:00:00

abstract：:Sera from patients with systemic infections caused by the opportunistic fungus Trichosporon beigelii have been shown to cross-react with anticryptococcal antibodies. We quantitatively compared the amounts of antigen produced and examined the expression of O-acetyl epitopes from 35 strains of T. beigelii isolated from ...

journal_title：Journal of clinical microbiology

authors： Lyman CA,Devi SJ,Nathanson J,Frasch CE,Pizzo PA,Walsh TJ

更新日期：1995-01-01 00:00:00

abstract：:Global HIV treatment programs need sensitive and affordable tests to monitor HIV drug resistance. We compared mutant detection by the oligonucleotide ligation assay (OLA), an economical and simple test, to massively parallel sequencing. Nonnucleoside reverse transcriptase inhibitor (K103N, V106M, Y181C, and G190A) and...

journal_title：Journal of clinical microbiology

authors： Beck IA,Deng W,Payant R,Hall R,Bumgarner RE,Mullins JI,Frenkel LM

更新日期：2014-07-01 00:00:00

abstract：:Of 121 AIDS patients, 12 (10%) had Toxoplasma gondii DNA detected by competitive PCR in their bronchoalveolar lavage samples. Quantitation of the PCR results showed a correlation between the parasite burden and the serum lactic dehydrogenase titer. Quantitative competitive PCR could be useful to assess the diagnosis a...

journal_title：Journal of clinical microbiology

authors： Bretagne S,Costa JM,Fleury-Feith J,Poron F,Dubreuil-Lemaire ML,Vidaud M

更新日期：1995-06-01 00:00:00

abstract：:Several species within the Burkholderia cepacia complex (BCC) have emerged as significant opportunistic pathogens of patients with cystic fibrosis (CF). BCC infection is typically associated with a poor clinical prognosis and decreased survival. These factors, combined with the existence of highly transmissible epidem...

journal_title：Journal of clinical microbiology

authors： Brown AR,Govan JR

更新日期：2007-06-01 00:00:00

abstract：:Candida famata (teleomorph Debaryomyces hansenii) has been described as a medically relevant yeast, and this species has been included in many commercial identification systems that are currently used in clinical laboratories. Among 53 strains collected during the SENTRY and ARTEMIS surveillance programs and previousl...

journal_title：Journal of clinical microbiology

authors： Castanheira M,Woosley LN,Diekema DJ,Jones RN,Pfaller MA

更新日期：2013-01-01 00:00:00

abstract：:The genotypes of 63 isolates of Aspergillus fumigatus obtained from three hospitals in different geographical areas and of eight culture collection strains were determined by multilocus enzyme electrophoresis. Twelve of the 17 enzymatic loci studied were polymorphic, giving rise to 48 different electrophoretic types. ...

journal_title：Journal of clinical microbiology

authors： Rodriguez E,De Meeüs T,Mallie M,Renaud F,Symoens F,Mondon P,Piens MA,Lebeau B,Viviani MA,Grillot R,Nolard N,Chapuis F,Tortorano AM,Bastide JM

更新日期：1996-10-01 00:00:00

abstract：:A comparison of three different commercial media was made to assess their recovery of anaerobic organisms from the blood stream. The three media used were the 50-ml brain heart infusion broth with added CO2 (Pfizer), the 50-ml Thiol broth with added CO2 (Difco), and the 50-ml prereduced, supplemented peptone broth in ...

journal_title：Journal of clinical microbiology

authors： Mangels JI,Lindberg LH,Vosti KL

更新日期：1977-05-01 00:00:00

abstract：:Rotaviruses contain a double-stranded ribonucleic acid genome consisting of 11 segments. Gel electrophoresis separates genome segments and allows identification of strain differences. This electrophoretic typing technique was applied to rotavirus specimens from 116 children and 72 newborn babies. Between 1973 and 1979...

journal_title：Journal of clinical microbiology

authors： Rodger SM,Bishop RF,Birch C,McLean B,Holmes IH

更新日期：1981-02-01 00:00:00

abstract：:The value of performing routine aerobic subcultures of both vented and unvented blood culture bottles has not been evaluated critically. We studied 4,954 pairs of blood culture bottles consisting of one vented biphasic tryptic soy broth bottle (Roche Diagnostics) and one unvented Thiol broth bottle (Difco Laboratories...

journal_title：Journal of clinical microbiology

authors： Pfaller MA,Westfall LM,Murray PR

更新日期：1983-04-01 00:00:00

abstract：:The use of real-time quantitative PCR (5' nuclease PCR assay) as a tool to study the gastrointestinal microflora that adheres to the colonic mucosa was evaluated. We developed primers and probes based on the 16S ribosomal DNA gene sequences for the detection of Escherichia coli and Bacteroides vulgatus. DNA was isolat...

journal_title：Journal of clinical microbiology

authors： Huijsdens XW,Linskens RK,Mak M,Meuwissen SG,Vandenbroucke-Grauls CM,Savelkoul PH

更新日期：2002-12-01 00:00:00

abstract：:Blastomyces dermatitidis and Histoplasma capsulatum are dimorphic fungi that often cause self-limited respiratory infections. However, they may also cause severe disseminated disease, depending on the level of the exposure to the organism and the host immune status. In addition, patients with infections caused by thes...

journal_title：Journal of clinical microbiology

authors： Babady NE,Buckwalter SP,Hall L,Le Febre KM,Binnicker MJ,Wengenack NL

更新日期：2011-09-01 00:00:00

abstract：:Carbapenem-resistant Acinetobacter baumannii isolates were obtained from 24 patients between March and May 2004 at the Centre Hospitalier de Polynésie Française, Tahiti, French Polynesia. The isolates were multidrug resistant, produced the carbapenemase OXA-23, and belonged to a single clone presenting several subtype...

journal_title：Journal of clinical microbiology

authors： Naas T,Levy M,Hirschauer C,Marchandin H,Nordmann P

更新日期：2005-09-01 00:00:00

abstract：:Colonization of the stomach mucosa by Helicobacter pylori is a major cause of acute and chronic gastric pathologies in humans. Several H. pylori virulence genes that may play a role in its pathogenicity have been identified. The most important determinants are vacA and cagA in the cag pathogenicity island (cagPAI) gen...

journal_title：Journal of clinical microbiology

authors： Yamazaki S,Yamakawa A,Okuda T,Ohtani M,Suto H,Ito Y,Yamazaki Y,Keida Y,Higashi H,Hatakeyama M,Azuma T

更新日期：2005-08-01 00:00:00

abstract：:We present the case of a 77-year-old woman who developed an Actinomyces naeslundii infection of a hip prosthesis. The isolate grew well aerobically with 5% CO(2). Possible diagnostic problems may arise in the microbiological laboratory because aerobic growth is not sufficiently accounted for in some of the traditional...

journal_title：Journal of clinical microbiology

authors： Wüst J,Steiger U,Vuong H,Zbinden R

更新日期：2000-02-01 00:00:00

abstract：:Although Escherichia fergusonii has been identified for decades, it has rarely been recovered from clinical specimens and its clinical significance remains unclear. We describe a case of E. fergusonii bacteremia in a diabetic patient with pancreatic cancer. The isolate was confirmed by three commercial identification ...

journal_title：Journal of clinical microbiology

authors： Lai CC,Cheng A,Huang YT,Chung KP,Lee MR,Liao CH,Hsueh PR

更新日期：2011-11-01 00:00:00

abstract：:Many parts of the Salmonella rfb gene clusters which are responsible for biosynthesis of the oligosaccharide-repeating units of the O-antigenic lipopolysaccharide have recently been cloned and sequenced. On the basis of this knowledge, three sets of nucleotide primers were selected to target defined regions of the abe...

journal_title：Journal of clinical microbiology

authors： Luk JM,Kongmuang U,Reeves PR,Lindberg AA

更新日期：1993-08-01 00:00:00

abstract：:Transfusion-acquired cytomegalovirus (CMV) infections should be prevented in seronegative immunocompromised patients by providing blood products from donors who are also seronegative. Latex agglutination was investigated as a simple and rapid method for detecting antibody against CMV. Latex beads were coated with CMV ...

journal_title：Journal of clinical microbiology

authors： Adler SP,McVoy M,Biro VG,Britt WJ,Hider P,Marshall D

更新日期：1985-07-01 00:00:00

abstract：:From late 2003 to the end of 2005, the Health Protection Agency's national reference laboratories received approximately 1,600 referrals of Acinetobacter spp., including 419 and 58 examples, respectively, of two carbapenem-resistant Acinetobacter baumannii lineages, designated OXA-23 clones 1 and 2. Representatives of...

journal_title：Journal of clinical microbiology

authors： Coelho JM,Turton JF,Kaufmann ME,Glover J,Woodford N,Warner M,Palepou MF,Pike R,Pitt TL,Patel BC,Livermore DM

更新日期：2006-10-01 00:00:00

abstract：:The mumps virus is a negative-strand RNA virus in the family Paramyxoviridae. Mumps infection results in an acute illness with symptoms including fever, headache, and myalgia, followed by swelling of the salivary glands. Complications of mumps can include meningitis, deafness, pancreatitis, orchitis, and first-trimest...

journal_title：Journal of clinical microbiology

authors： Boddicker JD,Rota PA,Kreman T,Wangeman A,Lowe L,Hummel KB,Thompson R,Bellini WJ,Pentella M,Desjardin LE

更新日期：2007-09-01 00:00:00

abstract：:We examined the blastogenic responses of lymphocytes to Staphylococcus aureus antigen in 49 uninfected non-insulin-dependent diabetic subjects and in 56 uninfected nondiabetic subjects. We found a significantly decreased response to this antigen in the diabetic patients (P less than 0.05). Diabetics whose blood sugar ...

journal_title：Journal of clinical microbiology

authors： Casey J,Sturm C Jr

更新日期：1982-01-01 00:00:00

abstract：:To study whether pathogenic clusters of Borrelia burgdorferi sensu lato strains occur, we typed 136 isolates, cultured from specimens from patients (n = 49) with various clinical entities and from ticks (n = 83) or dogs (n = 4) from different geographic regions, by randomly amplified polymorphic DNA (RAPD) fingerprint...

journal_title：Journal of clinical microbiology

authors： Wang G,van Dam AP,Spanjaard L,Dankert J

更新日期：1998-03-01 00:00:00