Vancomycin-resistant enterococci colonizing the intestinal tracts of hospitalized patients.

Abstract:

:A point prevalence culture survey was carried out to investigate the prevalence of fecal carriage of vancomycin-resistant enterococci (VRE) among patients admitted to an 800-bed general hospital where no VRE had been isolated previously. Twenty-two of 636 patients (3.5%) were found to be VRE carriers. Eighteen strains were identified as Enterococcus faecium, three were identified as Enterococcus gallinarum, and one was identified as Enterococcus faecalis. The susceptibilities of the enterococci to ampicillin, vancomycin, and teicoplanin were determined by the disk diffusion and the agar dilution methods. High-level resistance (HLR) to gentamicin and streptomycin was determined by the agar screening method. Eighteen strains (82%) were highly resistant to vancomycin, and four strains (18%) were moderately resistant to vancomycin. Five strains were susceptible to teicoplanin (23%; MICs, < or = 8 micrograms/ml). Only one strain (4.5%, E. faecium) showed HLR to gentamicin, and six strains (27%) showed HLR to streptomycin (one E. faecalis and five E. faecium strains). All 18 E. faecium and 1 E. faecalis strain carried the vanA gene, and 3 E. gallinarum strains carried the vanC gene. An epidemiological investigation revealed several risk factors for VRE colonization: hospitalization and duration of stay in the hematology department and prior vancomycin treatment. The study demonstrates that the patient's gastrointestinal tract is a possible reservoir for VRE, even in hospitals where VRE infections have not yet been observed. Therefore, we conclude that infection control precautions and restriction of glycopeptide usage may be key issues in limiting the emergence and spread of nosocomial VRE infections.

journal_name

J Clin Microbiol

authors

Gordts B,Van Landuyt H,Ieven M,Vandamme P,Goossens H

doi

10.1128/JCM.33.11.2842-2846.1995

subject

Has Abstract

pub_date

1995-11-01 00:00:00

pages

2842-6

issue

11

eissn

0095-1137

issn

1098-660X

journal_volume

33

pub_type

杂志文章
  • Oropharyngeal colonization of HIV-infected outpatients in Taiwan by yeast pathogens.

    abstract::Among 234 isolates comprising 26 different Candida species colonizing the oropharynx of 181 (54.3% of 399 surveyed) HIV-infected outpatients, 27 (11.7%) were fluconazole resistant. Antibacterial treatment was associated with increased rates of yeast colonization, while antiretroviral therapy and pneumococcal vaccinati...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00500-10

    authors: Yang YL,Hung CC,Wang AH,Tseng FC,Leaw SN,Tseng YT,Su CL,Chen HT,Lauderdale TL,Lo HJ

    更新日期:2010-07-01 00:00:00

  • Molecular analysis of bacterial species associated with childhood caries.

    abstract::Although substantial epidemiologic evidence links Streptococcus mutans to caries, the pathobiology of caries may involve more complex communities of bacterial species. Molecular methods for bacterial identification and enumeration now make it possible to more precisely study the microbiota associated with dental carie...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.40.3.1001-1009.2002

    authors: Becker MR,Paster BJ,Leys EJ,Moeschberger ML,Kenyon SG,Galvin JL,Boches SK,Dewhirst FE,Griffen AL

    更新日期:2002-03-01 00:00:00

  • Comparison of ribotyping and multilocus enzyme electrophoresis for subtyping of Listeria monocytogenes isolates.

    abstract::Ribotyping was compared with multilocus enzyme electrophoresis (MEE) for subtyping 305 Listeria monocytogenes isolates from clinical and nonclinical sources. For ribotyping, EcoRI-restricted genomic DNA fragments of L. monocytogenes strains were separated by agarose gel electrophoresis, and Southern blots were probed ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.32.12.2936-2943.1994

    authors: Graves LM,Swaminathan B,Reeves MW,Hunter SB,Weaver RE,Plikaytis BD,Schuchat A

    更新日期:1994-12-01 00:00:00

  • Evaluation of confirmatory strategies for detection of type-specific antibodies against herpes simplex virus type 2.

    abstract::In this study, the optimal combination of three commercial glycoprotein G-2 (gG-2)-based herpes simplex virus type 2 (HSV-2) type-specific enzyme-linked immunosorbent assays (Euroimmun anti-HSV-2 immunoglobulin G [IgG] ELISA [Eu2], Gull HSV-2-specific IgG ELISA [Gu2], and Radim HSV-2 IgG ELISA [Ra2]) and one gG-2-base...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.40.2.407-413.2002

    authors: Eing BR,Lippelt L,Lorentzen EU,Hafezi W,Schlumberger W,Steinhagen K,Kühn JE

    更新日期:2002-02-01 00:00:00

  • Value of examining multiple sputum specimens in the diagnosis of pulmonary tuberculosis.

    abstract::To objectively assess the value of examining multiple sputum specimens in maximizing the sensitivity of detection of Mycobacterium tuberculosis, we retrospectively reviewed the acid-fast bacillus smear and culture results of patients diagnosed with culture-proven pulmonary tuberculosis (TB) at Hennepin County Medical ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.36.2.467-469.1998

    authors: Nelson SM,Deike MA,Cartwright CP

    更新日期:1998-02-01 00:00:00

  • Investigation of apparent false-positive urine latex particle agglutination tests for the detection of group B streptococcus antigen.

    abstract::In our nursery, we identified neonates with positive urine latex particle agglutination (LPA) tests for group B streptococcus (GBS) antigen who did not have corroborating cultural evidence of infection. To investigate the mechanisms underlying these apparent false-positive reactions, we examined the urine LPA test in ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.27.10.2214-2217.1989

    authors: Harris MC,Deuber C,Polin RA,Nachamkin I

    更新日期:1989-10-01 00:00:00

  • Unique p24 epitope marker to identify multiple human immunodeficiency virus variants in blood from the same individuals.

    abstract::The human immunodeficiency virus (HIV) was isolated from the blood of 192 of 410 seropositive individuals. Original isolations were made in peripheral blood mononuclear cell (PBMC) cultures, and only one-fifth of the HIV isolates could be adapted to replicate in continuous T-cell lines. Of the 192 HIV isolates, 42 had...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.25.8.1411-1415.1987

    authors: Bolton V,Pedersen NC,Higgins J,Jennings M,Carlson J

    更新日期:1987-08-01 00:00:00

  • Antigenic characteristics of Borrelia burgdorferi isolates from ixodid ticks in California.

    abstract::Twenty (1.4%) of 1,421 adult Ixodes pacificus ticks and 2 (20%) of 10 adult Ixodes neotomae ticks collected in five counties of northern California were found to contain spirochetes by direct immunofluorescence examination of their tissues with a polyvalent conjugate. Borreliae isolated from the tissues of nine of the...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.27.10.2344-2349.1989

    authors: Lane RS,Pascocello JA

    更新日期:1989-10-01 00:00:00

  • Numerical analysis of electrophoretic protein patterns of methicillin-resistant strains of Staphylococcus aureus.

    abstract::A total of 50 strains of Staphylococcus aureus, including 41 methicillin-resistant S. aureus (MRSA) strains, were characterized by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins. The protein patterns contained 40-50 discrete bands and were highly reproducible. Partial ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.27.11.2574-2581.1989

    authors: Costas M,Cookson BD,Talsania HG,Owen RJ

    更新日期:1989-11-01 00:00:00

  • Evaluation of the immunoglobulin G avidity test for diagnosis of toxoplasmic lymphadenopathy.

    abstract::Toxoplasmic lymphadenopathy (TL) is the most common clinical manifestation of acute acquired toxoplasma infection in normal individuals. The diagnosis is established by serologic methods and lymph node biopsy. Recently, tests for avidity of toxoplasma immunoglobulin G (IgG) antibodies have been introduced to help disc...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.42.10.4627-4631.2004

    authors: Montoya JG,Huffman HB,Remington JS

    更新日期:2004-10-01 00:00:00

  • Human herpesvirus 8 seroprevalence and evaluation of nonsexual transmission routes by detection of DNA in clinical specimens from human immunodeficiency virus-seronegative patients from central and southern Italy, with and without Kaposi's sarcoma.

    abstract::In order to investigate the seroprevalence of human herpesvirus 8 (HHV-8) infection in central and southern Italy, sera from human immunodeficiency virus (HIV)-seronegative subjects, with and without Kaposi's sarcoma (KS), were analyzed by immunofluorescence assay, using BC-3, a cell line latently infected with HHV-8....

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.37.4.1150-1153.1999

    authors: Cattani P,Capuano M,Cerimele F,La Parola IL,Santangelo R,Masini C,Cerimele D,Fadda G

    更新日期:1999-04-01 00:00:00

  • Usefulness of the BACTEC MGIT 960 system for isolation of mycobacterium tuberculosis from sputa subjected to long-term storage.

    abstract::The recovery of Mycobacterium tuberculosis from sputa positive or negative for acid-fast bacilli that were stored for 17 +/- 7 days and inoculated in the BACTEC MGIT 960 system (MGIT) was higher than that from sputa inoculated in Lowenstein-Jensen medium. MGIT is useful for isolation of M. tuberculosis from sputa subj...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01985-06

    authors: Pardini M,Varaine F,Bonnet M,Orefici G,Oggioni MR,LONG-DRUG Study Group.,Fattorini L

    更新日期:2007-02-01 00:00:00

  • Variable serum immunoglobulin responses against different Borrelia burgdorferi sensu lato species in a population at risk for and patients with Lyme disease.

    abstract::Borrelia burgdorferi sensu lato species display considerable antigenic polymorphism. In order to evaluate the importance of this antigenic heterogeneity in the serodiagnosis of Lyme disease, the serum immunoglobulin G response in 148 healthy individuals from an area in northern Sweden where Lyme disease is endemic and...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.33.6.1473-1478.1995

    authors: Bunikis J,Olsén B,Westman G,Bergstroöm S

    更新日期:1995-06-01 00:00:00

  • Line probe assay for monitoring drug resistance in hepatitis B virus-infected patients during antiviral therapy.

    abstract::Since the introduction of antiviral compounds such as lamivudine and famciclovir in the treatment schedules of patients with chronic hepatitis B virus (HBV) infection, the accumulation of a variety of mutations in the HBV polymerase gene has been observed. The selection of these mutations is generally considered the c...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.38.2.702-707.2000

    authors: Stuyver L,Van Geyt C,De Gendt S,Van Reybroeck G,Zoulim F,Leroux-Roels G,Rossau R

    更新日期:2000-02-01 00:00:00

  • Three variants of Corynebacterium diphtheriae subsp. mitis (Belfanti) isolated from a throat specimen.

    abstract::Three variants of a Corynebacterium diphtheriae subsp. mitis strain belfanti, two toxigenic (phage types 7 and 11) and one nontoxigenic (phage type 7), were simultaneously isolated from the throat of an adult. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:

    authors: Chang DN,Laughren GS,Chalvardjian NE

    更新日期:1978-12-01 00:00:00

  • Scopulariopsis, a poorly known opportunistic fungus: spectrum of species in clinical samples and in vitro responses to antifungal drugs.

    abstract::Ninety-nine isolates of clinical origin, tentatively identified as Scopulariopsis or Microascus, were morphologically and molecularly characterized by a combined analysis of the D1/D2 domains of the 28S rRNA gene and a fragment of the elongation factor 1-α gene (EF1-α) sequences. The most prevalent species was Scopula...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01927-13

    authors: Sandoval-Denis M,Sutton DA,Fothergill AW,Cano-Lira J,Gené J,Decock CA,de Hoog GS,Guarro J

    更新日期:2013-12-01 00:00:00

  • Comparison of blood agar, ampicillin blood agar, MacConkey-ampicillin-Tween agar, and modified cefsulodin-Irgasan-novobiocin agar for isolation of Aeromonas spp. from stool specimens.

    abstract::The performance of four media for the isolation of Aeromonas strains from stool specimens, the importance of ampicillin-susceptible Aeromonas strains in the selection of culture media, and the usefulness of beta-hemolysis in screening blood-containing media for Aeromonas strains were evaluated in two phases. In the fi...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.26.9.1738-1740.1988

    authors: Kelly MT,Stroh EM,Jessop J

    更新日期:1988-09-01 00:00:00

  • Single primer pair for amplifying segments of distinct Shiga-like-toxin genes by polymerase chain reaction.

    abstract::By using a single synthetic oligonucleotide primer pair in the polymerase chain reaction, we amplified specific Shiga-like-toxin (SLT) gene segments from DNAs of 20 clinical Escherichia coli isolates, irrespective of whether they produce SLT-I, SLT-II, or heretofore uncategorized SLTs. These segments were not detectab...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.27.12.2751-2757.1989

    authors: Karch H,Meyer T

    更新日期:1989-12-01 00:00:00

  • Evaluation of a disk method for detection of hippurate hydrolysis by Campylobacter spp.

    abstract::A disk method for detecting hippurate hydrolysis by Campylobacter spp. was evaluated and compared with the conventional tube test used at the Centers for Disease Control (CDC) (G.K. Morris and C.M. Patton, p. 302-308, in E.H. Lennette, A. Balows, W.J. Hausler, Jr., and H.J. Shadomy, ed., Manual of Clinical Microbiolog...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.27.2.359-360.1989

    authors: Cacho JB,Aguirre PM,Hernanz A,Velasco AC

    更新日期:1989-02-01 00:00:00

  • Assessment of mycobacterial interspersed repetitive unit-QUB markers to further discriminate the Beijing genotype in a population-based study of the genetic diversity of Mycobacterium tuberculosis clinical isolates from Okinawa, Ryukyu Islands, Japan.

    abstract::The present investigation focused on genetic diversity and drug resistance of 101 Mycobacterium tuberculosis strains isolated between July 2003 and February 2005 in the Okinawa prefecture, Ryukyu Islands, Japan. A high rate of clustering (87%, eight clusters, 2 to 69 strains/cluster) was observed upon spoligotyping; m...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00348-07

    authors: Millet J,Miyagi-Shiohira C,Yamane N,Sola C,Rastogi N

    更新日期:2007-11-01 00:00:00

  • Pneumococcal pspA sequence types of prevalent multiresistant pneumococcal strains in the United States and of internationally disseminated clones.

    abstract::In a recent genotypic survey of beta-lactam-resistant pneumococci recovered in different areas of United States during 1997, eight clonal types that each represented 3 to 40 isolates accounted for 134 of 144 isolates (G. Gherardi, C. Whitney, R. Facklam, and B. Beall, J. Infect. Dis. 181:216-229, 2000). We determined ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.38.10.3663-3669.2000

    authors: Beall B,Gherardi G,Facklam RR,Hollingshead SK

    更新日期:2000-10-01 00:00:00

  • Outbreak of Candida parapsilosis endophthalmitis after cataract extraction and intraocular lens implantation.

    abstract::Between November 1983 and January 1984, 13 cases of Candida parapsilosis endophthalmitis occurred in Florida, Georgia, and Tennessee in patients who had had an intraocular lens implantation (IOLI) or cataract extraction with an IOLI. This outbreak followed the introduction in July 1983 of a new brand of balanced salt ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.24.4.625-628.1986

    authors: McCray E,Rampell N,Solomon SL,Bond WW,Martone WJ,O'Day D

    更新日期:1986-10-01 00:00:00

  • Indirect enzyme-linked immunosorbent assay for detection of Brucella melitensis-specific antibodies in goat milk.

    abstract::Brucella melitensis is the cause of brucellosis in sheep and goats, which often results in abortion. Few cases of B. melitensis infection in goats have occurred in the United States over the last 25 years. However, vigilance must be maintained, as it is for the bovine milk industry, to ensure that brucellosis is not i...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.43.2.721-725.2005

    authors: Funk ND,Tabatabai LB,Elzer PH,Hagius SD,Martin BM,Hoffman LJ

    更新日期:2005-02-01 00:00:00

  • New Nocardia taxon among isolates of Nocardia brasiliensis associated with invasive disease.

    abstract::Nocardia brasiliensis, the second most frequently isolated aerobic actinomycete in the clinical laboratory, is usually associated with localized cutaneous infections. However, 22% of 238 N. brasiliensis isolates from the United States and 12% of 66 isolates from Queensland, Australia, which had been collected over a 1...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.33.6.1528-1533.1995

    authors: Wallace RJ Jr,Brown BA,Blacklock Z,Ulrich R,Jost K,Brown JM,McNeil MM,Onyi G,Steingrube VA,Gibson J

    更新日期:1995-06-01 00:00:00

  • Species-specific identification of and distinction between Borrelia burgdorferi genomic groups by using 16S rRNA-directed oligonucleotide probes.

    abstract::Examination of a number of previously published aligned Borrelia 16S rRNA sequences revealed the presence of regions which could serve as oligonucleotide probe targets for both species-specific identification of Borrelia burgdorferi and distinction between genomic groups. Total cellular RNA isolated from Borrelia cult...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.30.3.628-632.1992

    authors: Marconi RT,Lubke L,Hauglum W,Garon CF

    更新日期:1992-03-01 00:00:00

  • Detection of respiratory syncytial virus antigen in nasal washings by Abbott TestPack enzyme immunoassay.

    abstract::We compared the new Abbott TestPack (TP) respiratory syncytial virus (RSV) enzyme immunoassay (EIA) with cell culture and two commercial RSV EIAs (from Abbott Diagnostics and Kallestad Laboratories) by using split samples of fresh nasal washings from children with suspected RSV disease. Two tubes of HEp-2 cells were i...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.28.6.1395-1397.1990

    authors: Wren CG,Bate BJ,Masters HB,Lauer BA

    更新日期:1990-06-01 00:00:00

  • Performance of a polymer-based DNA chip platform in detection and genotyping of human papillomavirus in clinical samples.

    abstract::Human papillomavirus (HPV) plays a key role in the development of cervical and laryngeal cancers. The aim of our study was to compare the performance of a new hydrogel-based HPV genotyping biochip assay (Biochip) to a commercially available and CE-marked conventional PCR followed by reverse hybridization (GenID-PCR). ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02080-08

    authors: Schenk T,Brandstetter T,Zur Hausen A,Alt-Mörbe J,Huzly D,Rühe J

    更新日期:2009-05-01 00:00:00

  • Detection of Giardia lamblia, Entamoeba histolytica/Entamoeba dispar, and Cryptosporidium parvum antigens in human fecal specimens using the triage parasite panel enzyme immunoassay.

    abstract::The Triage parasite panel (BIOSITE Diagnostics, San Diego, Calif.) is a new qualitative enzyme immunoassay (EIA) panel for the detection of Giardia lamblia, Entamoeba histolytica/E. dispar, and Cryptosporidium parvum in fresh or fresh, frozen, unfixed human fecal specimens. By using specific antibodies, antigens speci...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.38.9.3337-3340.2000

    authors: Garcia LS,Shimizu RY,Bernard CN

    更新日期:2000-09-01 00:00:00

  • Direct Detection of Emergent Fungal Pathogen Candida auris in Clinical Skin Swabs by SYBR Green-Based Quantitative PCR Assay.

    abstract::The recent emergence of the multidrug-resistant and pathogenic yeast Candida auris continues to cause public health concern worldwide. C. auris is alarming because it causes health care-associated outbreaks and can establish invasive infections with high mortality rates. Transmission between patients is facilitated by...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01337-18

    authors: Sexton DJ,Kordalewska M,Bentz ML,Welsh RM,Perlin DS,Litvintseva AP

    更新日期:2018-11-27 00:00:00

  • Inhibition of Pseudomonas aeruginosa slime production by staphylococcal extracellular product.

    abstract::Staphylococcus aureus produced an inhibitory factor that suppressed slime formation by Psuudomonas aeruginosa but had little effect on the growth of the organism. The inhibitory factor was not found in broth cultures but could be extracted from cultures grown on solid agar. The inhibitory factor moderately inhibited g...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.11.2.123-126.1980

    authors: Shibl AM,Al-Sowaygh IA

    更新日期:1980-02-01 00:00:00