Oropharyngeal colonization of HIV-infected outpatients in Taiwan by yeast pathogens.

abstract：:Campylobacter fetus subsp. fetus was isolated from the stools of two homosexual males. One was asymptomatic at the time of isolation. The other presented with diarrhea. Both isolates were initially grown at 42 degrees C. This organism should be included among the list of organisms that are found in homosexual males. ...

journal_title：Journal of clinical microbiology

authors： Devlin HR,McIntyre L

更新日期：1983-10-01 00:00:00

abstract：:Diagnosis of invasive aspergillosis (IA) is still a major problem in routine clinical practice. Early diagnosis is essential for a good patient prognosis. PCR is a highly sensitive method for the detection of nucleic acids and could play an important role in improving the diagnosis of fungal infections. Therefore, a n...

journal_title：Journal of clinical microbiology

authors： Springer J,Schloßnagel H,Heinz W,Doedt T,Soeller R,Einsele H,Loeffler J

更新日期：2012-08-01 00:00:00

abstract：:Dried blood spot (DBS) specimens were assessed as an alternative to plasma for human immunodeficiency virus type 1 (HIV-1) serotyping by V3 loop peptide enzyme immunoassay. Nested PCR capable of distinguishing HIV-1 subtypes B and E was used as the reference standard. Ninety-two percent of DBS samples were typeable as...

journal_title：Journal of clinical microbiology

authors： Chanbancherd P,Brown AE,Trichavaroj R,Tienamporn P,Puthakird P,Limpairojn N,VanCott TC,de Souza MS

更新日期：1999-03-01 00:00:00

abstract：:In 1991, multiresistant Escherichia coli O78:H10 strains caused an outbreak of urinary tract infections in Copenhagen, Denmark. The phylogenetic origin, clonal background, and virulence characteristics of the outbreak isolates, and their relationship to nonoutbreak O78:H10 strains according to these traits and resista...

journal_title：Journal of clinical microbiology

authors： Olesen B,Scheutz F,Andersen RL,Menard M,Boisen N,Johnston B,Hansen DS,Krogfelt KA,Nataro JP,Johnson JR

更新日期：2012-11-01 00:00:00

abstract：:Maternal prenatal screening for group B streptococci (GBS) followed by offering of intrapartum chemoprophylaxis to carriers is one of the strategies used to reduce the incidence of neonatal early-onset GBS infections. Culturing of vaginal and anorectal swab specimens in selective broth is the screening procedure recom...

journal_title：Journal of clinical microbiology

authors： Hansen SM,Sørensen UB

更新日期：2003-04-01 00:00:00

abstract：:High-resolution melt (HRM) analysis can identify sequence polymorphisms by comparing the melting curves of amplicons generated by real-time PCR amplification. We describe the application of this technique to identify Mycobacterium avium subspecies paratuberculosis types I, II, and III. The HRM approach was based on ty...

journal_title：Journal of clinical microbiology

authors： Castellanos E,Aranaz A,De Buck J

更新日期：2010-04-01 00:00:00

abstract：:To facilitate the routine identification of salmonellae and detailed studies of their lipopolysaccharides, we raised murine monoclonal antibodies against these organisms. We raised an immunoglobulin G1 antibody, MO2, which is specific for factor O2. By immunoblotting following sodium dodecyl sulfate-polyacrylamide gel...

journal_title：Journal of clinical microbiology

authors： Luk MC,Tsang RS,Ng MH

更新日期：1987-11-01 00:00:00

abstract：:Here we describe the first reported case of Nocardia beijingensis infection in the United States, made rarer by its presence in an immunocompetent patient. ...

journal_title：Journal of clinical microbiology

authors： Crozier JA,Andhavarapu S,Brumble LM,Sher T

更新日期：2014-07-01 00:00:00

abstract：:A cross-sectional study of nasopharyngeal colonization with Streptococcus pneumoniae was performed among 573 children attending 29 day-care centers (DCCs) in Norway prior to the start of mass vaccination with the heptavalent pneumococcal conjugate vaccine (PCV-7). A sensitive sampling method was employed, including tr...

journal_title：Journal of clinical microbiology

authors： Vestrheim DF,Høiby EA,Aaberge IS,Caugant DA

更新日期：2008-08-01 00:00:00

abstract：:We report the first case of peritonitis caused by Roseomonas mucosa which led to technique failure in an adolescent patient with HIV receiving peritoneal dialysis. Identification of the causative organism by 16S rRNA gene sequencing and phylogenetic analysis is described. ...

journal_title：Journal of clinical microbiology

authors： Boyd MA,Laurens MB,Fiorella PD,Mendley SR

更新日期：2012-11-01 00:00:00

abstract：:The rapid ID32A kit (bioMérieux Vitek, Inc., Hazelwood, Mo.) was evaluated for its ability to identify Peptostreptococcus species compared with conventional biochemical tests and gas-liquid chromatography (Virginia Polytechnic Institute), the current "gold standard" method. A total of 5 Peptostreptococcus American Typ...

journal_title：Journal of clinical microbiology

authors： Ng J,Ng LK,Chow AW,Dillon JA

更新日期：1994-05-01 00:00:00

abstract：:Genotypic typing by restriction fragment length polymorphism and pulsed-field gel electrophoresis showed that two neonates in a neonatal ward were infected with the same Campylobacter jejuni strain. Isolates from the mother and brother of the index patient were identical to each other but distinct from the neonatal ty...

journal_title：Journal of clinical microbiology

authors： Llovo J,Mateo E,Muñoz A,Urquijo M,On SL,Fernández-Astorga A

更新日期：2003-08-01 00:00:00

abstract：:Little is known about the sensitivity of the BinaxNOW pneumococcal urinary antigen (PUA) test for adult pneumococcal pneumonia caused by different serotypes. In this study, we aimed to analyze the trends in the sensitivity of the PUA test over a 15-year period (2001 to 2015) and to analyze its sensitivity for pneumoco...

journal_title：Journal of clinical microbiology

authors： Shoji H,Domenech A,Simonetti AF,González A,García-Somoza D,Cubero M,Martí S,Maeda M,Tubau F,Liñares J,Domínguez MA,Carratalà J,Ardanuy C

更新日期：2018-01-24 00:00:00

abstract：:We describe a new method which uses cold absolute methanol-prefixed cells for adherence of enteropathogenic Escherichia coli to HEp-2 cells. We found that a method using bacteria grown in Penassay broth to 10(6) to 10(7) CFU/ml and incubated with prefixed cells for 3 h at 37 degrees C, showed 100% sensitivity and spec...

journal_title：Journal of clinical microbiology

authors： Zepeda-Lopez HM,Gonzalez-Lugo GM

更新日期：1995-05-01 00:00:00

abstract：:For a positive coagulase test with certain isolates of Staphylococcus aureus, inoculation of 0.5 ml of coagulase plasma with at least two colonies and 24 h of incubation at 35 to 37 degrees C may be required. S. aureus does not multiply in coagulase plasma. There is also lot-to-lot variation in the sensitivity of coag...

journal_title：Journal of clinical microbiology

authors： Selepak ST,Witebsky FG

更新日期：1985-11-01 00:00:00

abstract：:A new rapid one-step immunochromatographic test using monoclonal antibodies for detection of Helicobacter pylori antigen in stool in children was evaluated on coded stool samples from 159 children (mean age, 9.7 +/- 5.0 years; 118 from Munich, 41 from Vienna): 86 children were H. pylori infected defined by positive cu...

journal_title：Journal of clinical microbiology

authors： Antos D,Crone J,Konstantopoulos N,Koletzko S

更新日期：2005-06-01 00:00:00

abstract：:During a 3-year period, all Pasteurella strains recovered at the Clinical Microbiological Laboratory, Lund, Sweden, were studied biochemically with respect to their relationship to the recently described taxa of this genus. Of 159 strains recovered from 146 infected humans, 95 were identified as Pasteurella multocida ...

journal_title：Journal of clinical microbiology

authors： Holst E,Rollof J,Larsson L,Nielsen JP

更新日期：1992-11-01 00:00:00

abstract：:The detection of class A serine-carbapenemases among species of Enterobacteriaceae remains a challenging issue. Methods of identification for routine use in clinical microbiology laboratories have not been standardized to date. We developed a novel screening methodology suitable for countries with high basal levels of...

journal_title：Journal of clinical microbiology

authors： Pasteran F,Mendez T,Guerriero L,Rapoport M,Corso A

更新日期：2009-06-01 00:00:00

abstract：:Human infections with Salmonella enterica subspecies enterica serovar Senftenberg are often associated with exposure to poultry flocks, farm environments, or contaminated food. The recent emergence of multidrug-resistant isolates has raised public health concerns. In this study, comparative genomics and phenotypic ana...

journal_title：Journal of clinical microbiology

authors： Abd El Ghany M,Shi X,Li Y,Ansari HR,Hill-Cawthorne GA,Ho YS,Naeem R,Pickard D,Klena JD,Xu X,Pain A,Hu Q

更新日期：2016-08-01 00:00:00

abstract：:Murex hybrid capture DNA assay (HCS) is a solution hybridization antibody capture assay for detection and quantitation of cytomegalovirus (CMV) DNA in leukocytes. To determine whether CMV HCS is sensitive enough to initiate and monitor antiviral therapy after allogeneic stem cell transplantation (SCT), 51 consecutive ...

journal_title：Journal of clinical microbiology

authors： Hebart H,Gamer D,Loeffler J,Mueller C,Sinzger C,Jahn G,Bader P,Klingebiel T,Kanz L,Einsele H

更新日期：1998-05-01 00:00:00

abstract：:The herpes simplex virus type 1 (HSV-1) genome is a linear double-stranded DNA of 152 kpb. It is divided into long and short regions of unique sequences termed U(L) and U(S), respectively, and these are flanked by regions of inverted internal and terminal repeats. Microsatellites are short tandem repeats of 1- to 6-nu...

journal_title：Journal of clinical microbiology

authors： Deback C,Boutolleau D,Depienne C,Luyt CE,Bonnafous P,Gautheret-Dejean A,Garrigue I,Agut H

更新日期：2009-03-01 00:00:00

abstract：:The single-nucleotide variation 823C to T (His275Tyr), responsible for oseltamivir drug resistance has been detected in some isolates of the influenza A/H1N1/2009 virus. Early detection of the presence of this oseltamivir-resistant strain allows prompt consideration of alternative treatment options. An isolated-probe-...

journal_title：Journal of clinical microbiology

authors： Lee HK,Lee CK,Loh TP,Tang JW,Tambyah PA,Koay ES

更新日期：2011-10-01 00:00:00

abstract：:To investigate the interaction of Limulus amoebocyte lysate (LAL) with gram-negative bacteria, soluble antigens from sonicated Pseudomonas aeruginosa were studied by various crossed-immunoelectrophoresis methods before and after reaction with LAL. Of 64 possible, at least 7 antigens were affected, as indicated by prec...

journal_title：Journal of clinical microbiology

authors： Baek L,Høiby N,Hertz JB,Espersen F

更新日期：1985-08-01 00:00:00

abstract：:An evaluation was undertaken to determine the optimal method for the in vitro susceptibility testing of 26 Nocardia asteroides complex isolates to the following antimicrobial agents: amikacin, ampicillin, amoxicillin-clavulanate, ceftriaxone, ciprofloxacin, erythromycin, imipenem, minocycline, and trimethoprim-sulfame...

journal_title：Journal of clinical microbiology

authors： Ambaye A,Kohner PC,Wollan PC,Roberts KL,Roberts GD,Cockerill FR 3rd

更新日期：1997-04-01 00:00:00

abstract：:A biotyping scheme using improved media and methods for the detection of hippurate hydrolysis, rapid H2S production, and DNA hydrolysis was applied to 1,826 cultures of Campylobacter jejuni, Campylobacter coli and "Campylobacter laridis" isolates from human and nonhuman sources. Four biotypes were identified among C. ...

journal_title：Journal of clinical microbiology

authors： Lior H

更新日期：1984-10-01 00:00:00

abstract：:To determine sensitivity and specificity of immunofluorescence assay (IFA) for detection of Helicobacter pylori, we studied 151 patients. Biopsies of gastric mucosae were obtained for culture, histological testing, and IFA. H. pylori serum antibodies were tested by enzyme-linked immunosorbent assay. IFA was done on Fo...

journal_title：Journal of clinical microbiology

authors： Rivera E,López-Vidal Y,Luqueño V,Ruiz-Palacios GM

更新日期：1991-08-01 00:00:00

abstract：:Early diagnosis of Neisseria gonorrhoeae infections is important with regard to patients' health and infectivity. We report the development of a specific and sensitive TaqMan assay for the detection of N. gonorrhoeae in clinical samples. The target sequence is a 76-bp fragment of the 5' untranslated region of the opa ...

journal_title：Journal of clinical microbiology

authors： Geraats-Peters CW,Brouwers M,Schneeberger PM,van der Zanden AG,Bruisten SM,Weers-Pothoff G,Boel CH,van den Brule AJ,Harmsen HG,Hermans MH

更新日期：2005-11-01 00:00:00

abstract：:Between 1 and 2% of the population in the developed world experiences a nonhealing or chronic wound characterized by an apparent arrest in a stage dominated by inflammatory processes. Lately, research groups have proposed that bacteria might be involved in and contribute to the lack of healing of these wounds. To inve...

journal_title：Journal of clinical microbiology

authors： Kirketerp-Møller K,Jensen PØ,Fazli M,Madsen KG,Pedersen J,Moser C,Tolker-Nielsen T,Høiby N,Givskov M,Bjarnsholt T

更新日期：2008-08-01 00:00:00

abstract：:Three forms of DNA-based typing procedures for Aspergillus fumigatus isolates have been developed over the last five years. The procedures are random amplification of polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP) detection, and Southern hybridizations with various repetitive sequence-based pr...

journal_title：Journal of clinical microbiology

authors： Anderson MJ,Gull K,Denning DW

更新日期：1996-01-01 00:00:00

abstract：:The detection of the human RNA viruses, calicivirus and astrovirus, requires high sensitivity and broad reactivity. A novel single-tube nested reverse transcription-PCR (RT-PCR) method is described here, in which all of the required reagents are included in the one tube; however, those required for the nested amplific...

journal_title：Journal of clinical microbiology

authors： Ratcliff RM,Doherty JC,Higgins GD

更新日期：2002-11-01 00:00:00