New, extended biotyping scheme for Campylobacter jejuni, Campylobacter coli, and "Campylobacter laridis".

abstract：:Recent studies have resulted in the proposal of a new species, Aeromonas schubertii (mannitol, sucrose, and indole negative), formerly termed Enteric Group 501, on the basis of the study of seven strains isolated from the southeastern and southwestern United States and Puerto Rico. We have isolated two phenotypically ...

journal_title：Journal of clinical microbiology

authors： Carnahan AM,Marii MA,Fanning GR,Pass MA,Joseph SW

更新日期：1989-08-01 00:00:00

abstract：:The clinical importance of nondiphtherial Corynebacterium, a ubiquitous member of the normal human microflora of the skin and mucous membrane, for ocular surface infections has been recognized recently. We performed an antimicrobial susceptibility test with Etest strips for three fluoroquinolones (ciprofloxacin, norfl...

journal_title：Journal of clinical microbiology

authors： Eguchi H,Kuwahara T,Miyamoto T,Nakayama-Imaohji H,Ichimura M,Hayashi T,Shiota H

更新日期：2008-02-01 00:00:00

abstract：:Group G Streptococcus has been implicated as a causative agent of pharyngitis in outbreak situations, but its role in endemic disease remains elusive. We found an unexpected inverse association of Streptococcus dysgalactiae subsp. equisimilis colonization and sore throat in a study of 2,194 children of 3 to 15 years o...

journal_title：Journal of clinical microbiology

authors： Tartof SY,Farrimond F,de Matos JA,Reis JN,Ramos RT,Andrade AN,dos Reis MG,Riley LW

更新日期：2011-01-01 00:00:00

abstract：:A case of zygomycosis caused by Apophysomyces elegans in a patient having 25% full-thickness burns is described. Amputation of the leg was necessary to control rapid tissue invasion. The fungus was isolated from soil in the burn environment. ...

journal_title：Journal of clinical microbiology

authors： Cooter RD,Lim IS,Ellis DH,Leitch IO

更新日期：1990-09-01 00:00:00

abstract：:An enzyme immunoassay (EIA) for serum immunoglobulin M (IgM) antibodies to rubella virus based on enzyme labeling of viral antigen was developed. The sensitivity of the EIA for the detection of recent rubella virus infection was evaluated by using 115 rubella-IgM-antibody-positive serum specimens, which were confirmed...

journal_title：Journal of clinical microbiology

authors： Seppänen H

更新日期：1990-04-01 00:00:00

abstract：:Multidrug-resistant opportunistic pathogens have become endemic to the veterinary hospital environment. Escherichia coli isolates resistant to 12 antibiotics were isolated from two dogs that were housed in the intensive care unit at The University of Georgia Veterinary Teaching Hospital within 48 h of each other. Revi...

journal_title：Journal of clinical microbiology

authors： Sanchez S,McCrackin Stevenson MA,Hudson CR,Maier M,Buffington T,Dam Q,Maurer JJ

更新日期：2002-10-01 00:00:00

abstract：:The aim of this study was to evaluate the seroprevalence of human herpesvirus 8 (HHV-8) in a group of 150 patients awaiting heart transplants and to detect HHV-8 seroconversion after transplantation. Four patients were HHV-8 seropositive before transplantation, and one of them developed Kaposi's sarcoma. One patient c...

journal_title：Journal of clinical microbiology

authors： Emond JP,Marcelin AG,Dorent R,Milliancourt C,Dupin N,Frances C,Agut H,Gandjbakhch I,Calvez V

更新日期：2002-06-01 00:00:00

abstract：:Evaluation of previously described chemically defined media for the growth of Legionella pneumophila showed that these media supported poor growth of several strains of L. pneumophila and did not support growth of certain of the Legionella species described later. Growth was stimulated by the dialysate from yeast extr...

journal_title：Journal of clinical microbiology

authors： Pine L,Franzus MJ,Malcolm GB

更新日期：1986-01-01 00:00:00

abstract：:Three molecular assays were evaluated for the direct detection of Mycobacterium tuberculosis complex bacteria in 125 respiratory and 22 nonrespiratory samples. The overall sensitivities obtained were as follows: GenoType MTBDRplus, 97.9%; GenoType Mycobacteria Direct, 93.7%; Gen-Probe Mycobacterium tuberculosis Amplif...

journal_title：Journal of clinical microbiology

authors： Neonakis IK,Gitti Z,Baritaki S,Petinaki E,Baritaki M,Spandidos DA

更新日期：2009-08-01 00:00:00

abstract：:Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) was collected from children with bullous impetigo in 2003 and 2004. One strain collected in 2003 was Panton-Valentine leucocidin (PVL) positive. In 2004, a multiple-drug-resistant PVL(+) CA-MRSA strain was isolated from an athlete with a cutaneou...

journal_title：Journal of clinical microbiology

authors： Takizawa Y,Taneike I,Nakagawa S,Oishi T,Nitahara Y,Iwakura N,Ozaki K,Takano M,Nakayama T,Yamamoto T

更新日期：2005-07-01 00:00:00

abstract：:The noninvasive diagnosis of amebic liver abscess is challenging, as most patients at the time of diagnosis do not have a concurrent intestinal infection with Entamoeba histolytica. Fecal testing for E. histolytica parasite antigen or DNA is negative in most patients. A real-time PCR assay was evaluated for detection ...

journal_title：Journal of clinical microbiology

authors： Haque R,Kabir M,Noor Z,Rahman SM,Mondal D,Alam F,Rahman I,Al Mahmood A,Ahmed N,Petri WA Jr

更新日期：2010-08-01 00:00:00

abstract：:The G and P types of 2,912 rotavirus-positive fecal specimens collected from eight geographical areas of the United Kingdom between 1995 and 1998 were determined by reverse transcription-PCR. Although 15 different G-P combinations were identified, G1P[8], G2P[4], G3P[8], and G4P[8] strains constituted 95% of all the r...

journal_title：Journal of clinical microbiology

authors： Iturriza-Gómara M,Green J,Brown DW,Ramsay M,Desselberger U,Gray JJ

更新日期：2000-12-01 00:00:00

abstract：:Herpes infections are among the most common sexually transmitted diseases and are the most common cause of genital ulcer disease in the United States. This study addresses the changing distribution of herpes simplex virus type 1 (HSV-1) and HSV-2 in patients presenting for evaluation of herpetic infections. Viral cult...

journal_title：Journal of clinical microbiology

authors： Ribes JA,Steele AD,Seabolt JP,Baker DJ

更新日期：2001-09-01 00:00:00

abstract：:The incidence of antimicrobial-resistant Neisseria gonorrhoeae continues to rise in Canada; however, antimicrobial resistance data are lacking for approximately 70% of gonorrhea infections that are diagnosed directly from clinical specimens by nucleic acid amplification tests (NAATs). We developed a molecular assay fo...

journal_title：Journal of clinical microbiology

authors： Peterson SW,Martin I,Demczuk W,Bharat A,Hoang L,Wylie J,Allen V,Lefebvre B,Tyrrell G,Horsman G,Haldane D,Garceau R,Wong T,Mulvey MR

更新日期：2015-07-01 00:00:00

abstract：:In this study, we determined the sequence of the EcoRI-PstI fragment of the plasmid pCVD432, also termed the enteroaggregative Escherichia coli (EAggEC) probe. A primer pair complementary to this probe was designed for PCR amplification of a 630-bp region. Comparison of the analysis of the EAggEC probe sequence with t...

journal_title：Journal of clinical microbiology

authors： Schmidt H,Knop C,Franke S,Aleksic S,Heesemann J,Karch H

更新日期：1995-03-01 00:00:00

abstract：:The aims of this study were to evaluate the use of molecular fingerprinting for assessment of bacterial diversity in canine duodenal juice and to evaluate the variation in the small intestinal microflora at repeated sampling. Two groups of dogs were used. Duodenal juice was collected from eight dogs euthanized for an ...

journal_title：Journal of clinical microbiology

authors： Suchodolski JS,Ruaux CG,Steiner JM,Fetz K,Williams DA

更新日期：2004-10-01 00:00:00

abstract：:A genotyping study of 285 Hybrid Capture 2 low-risk probe cocktail-positive specimens showed cross-reactivity with several untargeted human papillomavirus genotypes. Cross-reactivity was often clinically beneficial due to the detection of untargeted low-risk genotypes. A total of 8.4% of positive results, usually weak...

journal_title：Journal of clinical microbiology

authors： Poljak M,Kocjan BJ,Kovanda A,Lunar MM,Lepej SZ,Planinic A,Seme K,Vince A

更新日期：2009-08-01 00:00:00

abstract：:Animal bite wounds are often infected with bacteria from the animal's oral flora. We report what we believe to be the first case of bacteremia with Bacteroides pyogenes resulting from an infected cat bite. ...

journal_title：Journal of clinical microbiology

authors： Madsen IR,Justesen US

更新日期：2011-08-01 00:00:00

abstract：:The DNA relatedness and restriction fragment length polymorphism patterns of whole-cell DNA and the general phenotypic properties of 14 isolates of the form species Candida parapsilosis representing three diverse genetic groups were compared. The data confirm the unrelatedness of the three groups at the species level....

journal_title：Journal of clinical microbiology

authors： Roy B,Meyer SA

更新日期：1998-01-01 00:00:00

abstract：:Pyrazinamide is important in tuberculosis treatment, as it is bactericidal to semidormant mycobacteria not killed by other antituberculosis drugs. Pyrazinamide is also one of the cornerstone drugs retained in the treatment of multidrug-resistant tuberculosis (MDR-TB). However, due to technical difficulties, routine dr...

journal_title：Journal of clinical microbiology

authors： Mphahlele M,Syre H,Valvatne H,Stavrum R,Mannsåker T,Muthivhi T,Weyer K,Fourie PB,Grewal HM

更新日期：2008-10-01 00:00:00

abstract：:The extent and importance of genotype heterogeneity of Pneumocystis carinii f. sp. hominis within lungs have not previously been investigated. Two hundred forty PCR clones obtained from respiratory specimens and lung segments from three patients with fatal P. carinii pneumonia were investigated to detect genetic diver...

journal_title：Journal of clinical microbiology

authors： Helweg-Larsen J,Lundgren B,Lundgren JD

更新日期：2001-10-01 00:00:00

abstract：:A qualitative multiplex reverse transcription (RT)-PCR and liquid hybridization assay for the detection of human enteroviruses, rhinoviruses, parechoviruses, and Aichi virus was developed. Furthermore, a separate assay for the recognition of hepatitis A virus was established to complement the test pattern so that all ...

journal_title：Journal of clinical microbiology

authors： Jokela P,Joki-Korpela P,Maaronen M,Glumoff V,Hyypiä T

更新日期：2005-03-01 00:00:00

abstract：:The specific identification of verocytotoxin (VT)-producing Escherichia coli (VTEC) requires the detection of VTs in bacterial culture filtrates or the detection of genes encoding these toxins in bacterial cells by specific DNA probes. The standard method for detecting these toxins involves a time-consuming, labor-int...

journal_title：Journal of clinical microbiology

authors： Basta M,Karmali M,Lingwood C

更新日期：1989-07-01 00:00:00

abstract：:Monoclonal antibody (MAb)-based sandwich direct enzyme-linked immunosorbent assay (MSD-ELISA) methods that can detect foot-and-mouth disease virus (FMDV) antigens, both multiserotype (MSD-ELISA/MS) (for O, A, C, and Asia 1) and single-serotype (MSD-ELISA/SS) (for O, A, and Asia 1, specifically), were developed. MAb 1H...

journal_title：Journal of clinical microbiology

authors： Morioka K,Fukai K,Yoshida K,Yamazoe R,Onozato H,Ohashi S,Tsuda T,Sakamoto K

更新日期：2009-11-01 00:00:00

abstract：:The objective of the present study was the development of a diagnostic reverse transcription (RT)-PCR for the specific detection of enterovirus (EV) RNA in clinical specimens controlled by an internal control (IC) RNA. The IC RNA contains the same primer binding sites as EV RNA but has a different probe region. The IC...

journal_title：Journal of clinical microbiology

authors： Beld M,Minnaar R,Weel J,Sol C,Damen M,van der Avoort H,Wertheim-van Dillen P,van Breda A,Boom R

更新日期：2004-07-01 00:00:00

abstract：:Whole-genome sequencing (WGS) is a highly sensitive method for identifying genetic relatedness and transmission of Clostridioides difficile strains. Previous studies suggest that as few as 3 core genome single-nucleotide variants (SNVs) discriminate between genetically distinct isolates. Because a single C. difficile ...

journal_title：Journal of clinical microbiology

authors： Balaji A,Ozer EA,Kociolek LK

更新日期：2019-08-26 00:00:00

abstract：:A loop-mediated isothermal amplification (LAMP) method for the rapid detection of serotype 1 Marek's disease virus (MDV) was developed. The method used a set of three pairs of primers to amplify the MEQ gene for detecting serotype 1 MDV. The MDV LAMP method did not cross-react with serotype 2 and serotype 3, nor did t...

journal_title：Journal of clinical microbiology

authors： Angamuthu R,Baskaran S,Gopal DR,Devarajan J,Kathaperumal K

更新日期：2012-03-01 00:00:00

abstract：:A total of 189 Candida albicans isolates have been typed by multilocus enzyme electrophoresis. The results obtained confirm the clonal mode of reproduction of C. albicans. The C. albicans populations found in the oropharynx of human immunodeficiency virus (HIV)-infected patients, in the oropharynx of healthy carriers,...

journal_title：Journal of clinical microbiology

authors： Boerlin P,Boerlin-Petzold F,Goudet J,Durussel C,Pagani JL,Chave JP,Bille J

更新日期：1996-05-01 00:00:00

abstract：:Concentrations of Mycobacterium avium-M. intracellulare ranging from 10(-1) to 10(3) CFU/ml were added to blood, placed in Isolator tubes, and held at room temperature for intervals ranging from 4 h to 56 days before being processed (centrifugation and culture on Middlebrook 7H10 agar). At all concentrations tested, M...

journal_title：Journal of clinical microbiology

authors： von Reyn CF,Hennigan S,Niemczyk S,Jacobs NJ

更新日期：1991-06-01 00:00:00

abstract：:Contamination of broth cultures of acid-fast bacilli (AFB) by bacterial species other than Mycobacterium species frequently occurs. Many of these contaminated cultures require redecontamination and reincubation before the appropriate tests can be performed for identification, significantly affecting the turnaround tim...

journal_title：Journal of clinical microbiology

authors： Zheng X,Pang M,Engler HD,Tanaka S,Reppun T

更新日期：2001-10-01 00:00:00