Guanine is a growth factor for Legionella species.

Abstract:

:Evaluation of previously described chemically defined media for the growth of Legionella pneumophila showed that these media supported poor growth of several strains of L. pneumophila and did not support growth of certain of the Legionella species described later. Growth was stimulated by the dialysate from yeast extract but not by the nondialyzable fraction. Further investigations indicated that the active factors from the yeast extract dialysate were purine or pyrimidine derivatives, and certain known purines and pyrimidines were found to stimulate growth. Of these, guanine universally stimulated growth of all Legionella strains and was a growth requirement for several of the species tested. A balanced, N-(2-acetamido)-2-aminoethanesulfonic acid-buffered, chemically defined medium having guanine or a purine-pyrimidine mix is presented for the general growth of Legionella species.

journal_name

J Clin Microbiol

authors

Pine L,Franzus MJ,Malcolm GB

doi

10.1128/JCM.23.1.163-169.1986

subject

Has Abstract

pub_date

1986-01-01 00:00:00

pages

163-9

issue

1

eissn

0095-1137

issn

1098-660X

journal_volume

23

pub_type

杂志文章
  • Purification of the 115-kilodalton exoantigen of Cryptococcus neoformans and its recognition by immune sera.

    abstract::A 115-kDa exoantigen produced by Cryptococcus neoformans recognized by the previously described murine monoclonal antibody 7C9 has been purified from culture filtrate by a combination of membrane ultrafiltration, isoelectric focusing, and preparative gel electrophoresis. It is produced in late-log-phase cultures and i...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.31.2.335-339.1993

    authors: Hamilton AJ,Goodley J

    更新日期:1993-02-01 00:00:00

  • Comparison of broth microdilution, E Test, and agar dilution methods for antibiotic susceptibility testing of Campylobacter jejuni and Campylobacter coli.

    abstract::A standardized broth microdilution method was compared to the E test and an agar dilution method for the antimicrobial susceptibility testing of Campylobacter jejuni and C. coli isolates. A group of 47 human clinical isolates, 37 isolates from retail poultry, and 29 isolates from living turkeys (total, 113 isolates) w...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.41.3.1062-1068.2003

    authors: Luber P,Bartelt E,Genschow E,Wagner J,Hahn H

    更新日期:2003-03-01 00:00:00

  • Genomic analysis of Mycobacterium bovis and other members of the Mycobacterium tuberculosis complex by isoenzyme analysis and pulsed-field gel electrophoresis.

    abstract::Initially, multilocus enzyme electrophoresis was used to examine genetic relationships among 63 isolates of Mycobacterium bovis and 13 other members of the M. tuberculosis complex. The isolates were divided into five electrophoretic types, with a mean genetic diversity of 0.1. The strains were genetically homogenous, ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.34.5.1136-1142.1996

    authors: Feizabadi MM,Robertson ID,Cousins DV,Hampson DJ

    更新日期:1996-05-01 00:00:00

  • Isolation and characterization of dually reactive strains of group a rotavirus from hospitalized children.

    abstract::Seven rotavirus strains dually reactive to serotype G1- and G2-specific monoclonal antibodies (MAbs) from hospitalized children with rotavirus diarrhea were culture adapted. Six strains were neutralized with G1 antiserum to a higher titer than that of G2, and one was neutralized with G1 and G2 antisera to equal titers...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.41.11.5267-5269.2003

    authors: Ranshing SS,Kelkar SD

    更新日期:2003-11-01 00:00:00

  • First report of Nocardia beijingensis infection in an immunocompetent host in the United States.

    abstract::Here we describe the first reported case of Nocardia beijingensis infection in the United States, made rarer by its presence in an immunocompetent patient. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00588-14

    authors: Crozier JA,Andhavarapu S,Brumble LM,Sher T

    更新日期:2014-07-01 00:00:00

  • Clinical trial comparing bacitracin with Strep-A-Chek for accuracy and turnaround time in the presumptive identification of Streptococcus pyogenes.

    abstract::In a clinical trial, Strep-A-Chek (a 10-min chromogenic test) was compared with the bacitracin disk susceptibility test for accuracy and turnaround time in the presumptive identification of Streptococcus pyogenes. Among 461 isolates of beta-hemolytic streptococci (344 throat isolates and 117 isolates from other sites)...

    journal_title:Journal of clinical microbiology

    pub_type: 临床试验,杂志文章

    doi:10.1128/JCM.24.3.431-434.1986

    authors: Yajko DM,Lawrence J,Nassos P,Young J,Hadley WK

    更新日期:1986-09-01 00:00:00

  • Interpretation of spectrophotometric absorbance values to define results of enzyme-linked immunosorbent assays.

    abstract::An enzyme-linked immunosorbent assay method was used to classify bovine serum as positive, negative, or doubtful for antibodies to Brucella abortus. Spectrophotometric data from assays of 64 serologically positive and 32 serologically negative bovine sera were analyzed statistically to define the range of spectrophoto...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.11.4.398-401.1980

    authors: Heck FC,Williams JD,Pruett J

    更新日期:1980-04-01 00:00:00

  • In vitro antimicrobial spectrum, occurrence of synergy, and recommendations for dilution susceptibility testing concentrations of the cefoperazone-sulbactam combination.

    abstract::Broth microdilution tests and an antimicrobial interaction (synergy) studies using various combinations of cefoperazone and sulbactam were performed in an effort to determine the most appropriate in vitro dilution test system. The test results with cefoperazone and sulbactam were categorized as synergistic (complete o...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.25.9.1725-1729.1987

    authors: Jones RN,Barry AL,Packer RR,Gregory WW,Thornsberry C

    更新日期:1987-09-01 00:00:00

  • Armored RNA as virus surrogate in a real-time reverse transcriptase PCR assay proficiency panel.

    abstract::In recent years testing responsibilities for high-consequence pathogens have been expanded from national reference laboratories into networks of local and regional laboratories in order to support enhanced disease surveillance and to test for surge capacity. This movement of testing of select agents and high-consequen...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.44.1.67-70.2006

    authors: Hietala SK,Crossley BM

    更新日期:2006-01-01 00:00:00

  • Outbreak of carbapenem-resistant Acinetobacter baumannii producing the carbapenemase OXA-23 in a tertiary care hospital of Papeete, French Polynesia.

    abstract::Carbapenem-resistant Acinetobacter baumannii isolates were obtained from 24 patients between March and May 2004 at the Centre Hospitalier de Polynésie Française, Tahiti, French Polynesia. The isolates were multidrug resistant, produced the carbapenemase OXA-23, and belonged to a single clone presenting several subtype...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.43.9.4826-4829.2005

    authors: Naas T,Levy M,Hirschauer C,Marchandin H,Nordmann P

    更新日期:2005-09-01 00:00:00

  • Aminopeptidase activity in arginine-utilizing Mycoplasma spp.

    abstract::In an examination of arginine-utilizing Mycoplasma species, simple fluorogenic and chromogenic tests were used to demonstrate high levels of arginine aminopeptidase activity in unwashed, unconcentrated broth and agar cultures in all strains of 5 species and low levels of activity in all strains of 14 species. Four spe...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.21.5.859-860.1985

    authors: Ball HJ,Neill SD,Reid RL

    更新日期:1985-05-01 00:00:00

  • Semiautomation of multilocus sequence typing for the characterization of clinical isolates of Neisseria meningitidis.

    abstract::The Scottish Meningococcus and Pneumococcus Reference Laboratory (SMPRL) provides a national service for the laboratory confirmation of meningococcal and pneumococcal disease in Scotland. Part of this service includes the serogrouping of meningococcal isolates followed by typing and subtyping. The procedures for this ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.39.9.3066-3071.2001

    authors: Clarke SC,Diggle MA,Edwards GF

    更新日期:2001-09-01 00:00:00

  • Fatal case of endocarditis due to Weissella confusa.

    abstract::This is the first reported case of endocarditis due to the Lactobacillus-like vancomycin-resistant gram-positive bacillus Weissella confusa. Full identification and susceptibility testing of Lactobacillus-like organisms recovered in blood culture should be performed for patients with clinical presentations that sugges...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.41.5.2237-2239.2003

    authors: Flaherty JD,Levett PN,Dewhirst FE,Troe TE,Warren JR,Johnson S

    更新日期:2003-05-01 00:00:00

  • Identification of Bordetella pertussis in a critically ill human immunodeficiency virus-infected patient by direct genotypical analysis of Gram-stained material and discrimination from B. holmesii by using a unique recA gene restriction enzyme site.

    abstract::Bordetella pertussis was diagnosed in a human immunodeficiency virus-infected patient by a newly developed method in which bacterial DNA is amplified directly from sputum Gram-stained slides. The validation of the method is described along with an additional new PCR-based assay that can distinguish between B. pertussi...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.42.2.847-849.2004

    authors: Vielemeyer O,Crouch JY,Edberg SC,Howe JG

    更新日期:2004-02-01 00:00:00

  • Bacteremia due to Clostridium hathewayi in a patient with acute appendicitis.

    abstract::Clostridium hathewayi is a newly described Clostridium species isolated from the feces of healthy human individuals, but its clinical significance is not known. We describe a case of human infection associated with C. hathewayi. The bacterium (strain HKU18) was isolated from the blood culture of a 39-year-old patient ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.42.12.5947-5949.2004

    authors: Woo PC,Lau SK,Woo GK,Fung AM,Yiu VP,Yuen KY

    更新日期:2004-12-01 00:00:00

  • Taxonomic Changes for Human and Animal Viruses, 2018 to 2020.

    abstract::The classification of viruses is relevant to a number of scientific and clinical disciplines, including the practice of diagnostic virology. Here, we provide an update to our previous review of taxonomic changes for disease-causing viruses in humans and vertebrate animals, covering changes between 2018 and 2020. Recen...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,评审

    doi:10.1128/JCM.01932-20

    authors: Loeffelholz MJ,Fenwick BW

    更新日期:2021-01-21 00:00:00

  • Identification of Yersinia enterocolitica O15 lipopolysaccharide as a rough antigen.

    abstract::Lipopolysaccharide (LPS) of a reference strain of Yersinia enterocolitica serovar O15, Ye614, was examined for its chemical structure and antigenicity. The O15 LPS was partially hydrolyzed with diluted acetic acid and fractionated on a Sephadex G-50 column. Only one peak was detected at fractions where the R-core olig...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.24.2.272-274.1986

    authors: Kawaoka Y,Wauters G,Otsuki K,Tsubokura M

    更新日期:1986-08-01 00:00:00

  • Harmonization of Bordetella pertussis real-time PCR diagnostics in the United States in 2012.

    abstract::Real-time PCR (rt-PCR) is an important diagnostic tool for the identification of Bordetella pertussis, Bordetella holmesii, and Bordetella parapertussis. Most U.S. public health laboratories (USPHLs) target IS481, present in 218 to 238 copies in the B. pertussis genome and 32 to 65 copies in B. holmesii. The CDC devel...

    journal_title:Journal of clinical microbiology

    pub_type: 历史文章,杂志文章

    doi:10.1128/JCM.02368-14

    authors: Williams MM,Taylor TH Jr,Warshauer DM,Martin MD,Valley AM,Tondella ML

    更新日期:2015-01-01 00:00:00

  • Three new serotypes of Salmonella.

    abstract::Three new Salmonella serotypes belonging to Kauffmann's subgenus I (F. Kauffmann, The Bacteriology of Enterobacteriaceae, 1966) were identified. These serotypes were Salmonella brazos 6,14,18:a:e,n,z15, Salmonella midway 6,14,24:d:1,7, and Salmonella balboa 48a, 48b:z41:monophasic. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.11.6.748-749.1980

    authors: Sutch KE,Blackburn BO

    更新日期:1980-06-01 00:00:00

  • Adiaspiromycosis causing respiratory failure and a review of human infections due to Emmonsia and Chrysosporium spp.

    abstract::We report a case of a 27-year-old male who presented with respiratory distress that required mechanical ventilation. Transbronchial biopsy revealed adiaspores of the fungus Emmonsia crescens within granulomata, a condition known as adiaspiromycosis. The patient received amphotericin products and corticosteroids, follo...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,评审

    doi:10.1128/JCM.00226-11

    authors: Anstead GM,Sutton DA,Graybill JR

    更新日期:2012-04-01 00:00:00

  • Rapid viral diagnosis of acute respiratory infections: comparison of enzyme-linked immunosorbent assay and the immunofluorescence technique for detection of viral antigens in nasopharyngeal secretions.

    abstract::Nasopharyngeal secretions from adults and children were obtained in Stockholm, Sweden, for routine diagnosis of influenza A virus, influenza B virus, respiratory syncytial (RS) virus, parainfluenza type 3 virus, and adenovirus infections by demonstration of viral antigens directly in the specimens. The cells in nasoph...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.22.5.757-760.1985

    authors: Grandien M,Pettersson CA,Gardner PS,Linde A,Stanton A

    更新日期:1985-11-01 00:00:00

  • Characterization of rough and smooth morphotypes of Mycobacterium abscessus isolates from clinical specimens.

    abstract::Mycobacterium abscessus, which consists of the two subspecies M. abscessus subspecies abscessus and M. abscessus subspecies bolletii, can produce rough or smooth colony morphologies. Here we analyzed 50 M. abscessus isolates cultured from the respiratory specimens of 34 patients, 28 (82%) of whom had cystic fibrosis (...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01249-13

    authors: Rüger K,Hampel A,Billig S,Rücker N,Suerbaum S,Bange FC

    更新日期:2014-01-01 00:00:00

  • Reverse transcriptase PCR diagnostic assay for the coronavirus associated with severe acute respiratory syndrome.

    abstract::Recent outbreaks of severe acute respiratory syndrome (SARS) have spurred intense research efforts around the world to deal with the serious threat to health posed by this novel coronavirus. A rapid, reliable diagnostic assay is needed for monitoring the spread of the disease. Here we report a method for eliminating f...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.42.5.1994-1999.2004

    authors: Hui RK,Zeng F,Chan CM,Yuen KY,Peiris JS,Leung FC

    更新日期:2004-05-01 00:00:00

  • Discovery of Novel Pneumococcal Serotype 35D, a Natural WciG-Deficient Variant of Serotype 35B.

    abstract::Pneumococcus (Streptococcus pneumoniae) remains a significant cause of morbidity and mortality, especially among those at the extremes of age. Its capsular polysaccharide is essential for systemic virulence. Over 90 serologically distinct pneumococcal capsular polysaccharides (serotypes) are recognized, but they are u...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00054-17

    authors: Geno KA,Saad JS,Nahm MH

    更新日期:2017-05-01 00:00:00

  • Genetic diversity of intimin genes of attaching and effacing Escherichia coli strains.

    abstract::In this study, we determined the sequences of four intimin variant genes detected in attaching and effacing Escherichia coli isolates of human origin. Three of them were novel and were designated eae-eta (eta), eae-iota (iota), and eae-kappa (kappa). The fourth was identical to the recently described eae-zeta (zeta), ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.40.12.4486-4492.2002

    authors: Zhang WL,Köhler B,Oswald E,Beutin L,Karch H,Morabito S,Caprioli A,Suerbaum S,Schmidt H

    更新日期:2002-12-01 00:00:00

  • Clinical significance of quantifying Pneumocystis jirovecii DNA by using real-time PCR in bronchoalveolar lavage fluid from immunocompromised patients.

    abstract::Quantitative PCR (qPCR) is more sensitive than microscopy for detecting Pneumocystis jirovecii in bronchoalveolar lavage (BAL) fluid. We therefore developed a qPCR assay and compared the results with those of a routine immunofluorescence assay (IFA) and clinical data. The assay included automated DNA extraction, ampli...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.06036-11

    authors: Botterel F,Cabaret O,Foulet F,Cordonnier C,Costa JM,Bretagne S

    更新日期:2012-02-01 00:00:00

  • Identification of Group B Streptococcus Capsule Type by Use of a Dual Phenotypic/Genotypic Assay.

    abstract::The group B streptococcus (GBS) capsular polysaccharide (CPS) is an important virulence factor which is also used for GBS typing. There are 10 CPS types (Ia, Ib, and II to IX). GBS that do not phenotypically type are considered nontypeable. All genes required for CPS synthesis are found on the GBS cps operon, which co...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00300-17

    authors: Alhhazmi A,Pandey A,Tyrrell GJ

    更新日期:2017-09-01 00:00:00

  • Immunoelectrophoresis for detection of polysaccharides in immune complexes.

    abstract::A procedure for detecting pneumococcal capsular polysaccharides in immune complexes is described. Separation of antigen from immune complexes is achieved by electrophoresis at 56 degrees C. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:

    authors: Coonrod JD,Leach RP

    更新日期:1978-08-01 00:00:00

  • Mycoplasma genitalium Prevalence, Coinfection, and Macrolide Antibiotic Resistance Frequency in a Multicenter Clinical Study Cohort in the United States.

    abstract::The prevalence rates of Mycoplasma genitalium infections and coinfections with other sexually transmitted organisms and the frequency of a macrolide antibiotic resistance phenotype were determined in urogenital specimens collected from female and male subjects enrolled in a multicenter clinical study in the United Sta...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,多中心研究

    doi:10.1128/JCM.01053-16

    authors: Getman D,Jiang A,O'Donnell M,Cohen S

    更新日期:2016-09-01 00:00:00

  • Molecular analysis of Malassezia microflora on the skin of atopic dermatitis patients and healthy subjects.

    abstract::Members of the genus Malassezia, lipophilic yeasts, are considered to be one of the exacerbating factors in atopic dermatitis (AD). We examined variation in cutaneous colonization by Malassezia species in AD patients and compared it with variation in healthy subjects. Samples were collected by applying transparent dre...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.39.10.3486-3490.2001

    authors: Sugita T,Suto H,Unno T,Tsuboi R,Ogawa H,Shinoda T,Nishikawa A

    更新日期:2001-10-01 00:00:00