Typing Candida albicans oral isolates from human immunodeficiency virus-infected patients by multilocus enzyme electrophoresis and DNA fingerprinting.

abstract：:We investigated a method to reduce oxygen tension and produce anaerobic conditions conveniently by coculture with a nonfermentative organism. The biochemical reactions of 94 strains representing 41 different species were determined using prereduced media and gas chromatography according to standard methods. The standa...

journal_title：Journal of clinical microbiology

authors： Hussain Z,Bürger H,Lannigan R,Groves DJ

更新日期：1984-02-01 00:00:00

abstract：:This study demonstrates that significant reproducibility problems can occur during routine use of the Abbott Laboratories LCx assay for Chlamydia trachomatis and Neisseria gonorrhoeae. These problems can go undetected by the quality control procedures outlined in the manufacturer's package insert. We outline here proc...

journal_title：Journal of clinical microbiology

authors： Gronowski AM,Copper S,Baorto D,Murray PR

更新日期：2000-06-01 00:00:00

abstract：:The aim of this study was to develop a PCR and reverse line blot hybridization (PCR-RLB) macroarray assay based on 16S-23S rRNA gene internal transcribed spacer sequences for the identification and differentiation of 34 mycobacterial species or subspecies. The performance of the PCR-RLB assay was assessed and validate...

journal_title：Journal of clinical microbiology

authors： Xiong L,Kong F,Yang Y,Cheng J,Gilbert GL

更新日期：2006-10-01 00:00:00

abstract：:Latex particle agglutination (LPA) testing for antigen to group B streptococcus (GBS) has been useful in the diagnosis of GBS sepsis in newborns. However, recent reports have demonstrated that the sensitivity of LPA assays may be as low as 27 to 54%. The purposes of the present study were to directly compare the abili...

journal_title：Journal of clinical microbiology

authors： Greenberg DN,Ascher DP,Yoder BA,Hensley DM,Heiman HS,Keith JF 3rd

更新日期：1995-01-01 00:00:00

abstract：:The performance of four media for the isolation of Aeromonas strains from stool specimens, the importance of ampicillin-susceptible Aeromonas strains in the selection of culture media, and the usefulness of beta-hemolysis in screening blood-containing media for Aeromonas strains were evaluated in two phases. In the fi...

journal_title：Journal of clinical microbiology

authors： Kelly MT,Stroh EM,Jessop J

更新日期：1988-09-01 00:00:00

abstract：:Given the rise in the incidence of invasive fungal infections (IFIs) and the expanding spectrum of fungal pathogens, early and accurate identification of the causative pathogen is essential. We developed a panfungal PCR assay that targets the internal transcribed spacer 1 (ITS1) region of the ribosomal DNA gene cluste...

journal_title：Journal of clinical microbiology

authors： Lau A,Chen S,Sorrell T,Carter D,Malik R,Martin P,Halliday C

更新日期：2007-02-01 00:00:00

abstract：:A human astrovirus (HAstV) strain from Kenya was characterized by nucleotide sequence analysis. Sequences from open reading frame 1a (ORF1a) clustered with genotype 6/7, those from ORF1b clustered with genotype 3, and those from ORF2 clustered with genotype 2. A recombination point in the ORF1b-ORF2 junction was ident...

journal_title：Journal of clinical microbiology

authors： Wolfaardt M,Kiulia NM,Mwenda JM,Taylor MB

更新日期：2011-02-01 00:00:00

abstract：:A total of 201 Mycoplasma pneumoniae clinical isolates from Beijing, China, isolated from 2008 to 2011, were clustered into 16 multiple-locus variable-number tandem-repeat analysis (MLVA) types, of which 6 new MLVA types have never been reported previously. Type 1 isolates based on p1 gene genotyping were mainly MLVA ...

journal_title：Journal of clinical microbiology

authors： Zhao F,Liu G,Cao B,Wu J,Gu Y,He L,Meng F,Zhu L,Yin Y,Lv M,Zhang J

更新日期：2013-02-01 00:00:00

abstract：:Quantitative microdilution plate hybridization was used to identify 22 Mycobacterium species. DNAs of clinical strains were rapidly extracted and labeled with photoreactive biotin. Labeled DNAs were distributed into wells of a microdilution plate in which reference DNAs had been immobilized. After 2 h of hybridization...

journal_title：Journal of clinical microbiology

authors： Kusunoki S,Ezaki T,Tamesada M,Hatanaka Y,Asano K,Hashimoto Y,Yabuuchi E

更新日期：1991-08-01 00:00:00

abstract：:The purpose of this study was to determine whether individual milk samples can replace serum samples for the detection of bovine herpesvirus 1 (BHV1) glycoprotein E (gE)-specific antibodies. Serum and milk samples were collected at the same time from cattle in BHV1-free herds, cattle in unvaccinated herds, and cattle ...

journal_title：Journal of clinical microbiology

authors： Wellenberg GJ,Verstraten ER,Mars MH,Van Oirschot JT

更新日期：1998-02-01 00:00:00

abstract：:Standard broth microdilution (with and without bovine serum albumin [BSA] supplementation), tube dilution, and agar dilution susceptibility tests were compared for determining ramoplanin MICs. With a data base of 246 clinical isolates of gram-positive bacteria from 33 U.S. sites, it was shown that (i) agar and tube di...

journal_title：Journal of clinical microbiology

authors： Kenny MT,Brackman MA

更新日期：1994-05-01 00:00:00

abstract：:Classifications of the Campylobacter fetus subspecies fetus and venerealis were first described in 1959 and were based on the source of isolation (intestinal versus genital) and the ability of the strains to proliferate in the genital tract of cows. Two phenotypic assays (1% glycine tolerance and H2S production) were ...

journal_title：Journal of clinical microbiology

authors： van der Graaf-van Bloois L,Miller WG,Yee E,Rijnsburger M,Wagenaar JA,Duim B

更新日期：2014-12-01 00:00:00

abstract：:While the majority of avian influenza virus (AIV) subtypes are classified as low-pathogenicity avian influenza viruses (LPAIV), the H5 and H7 subtypes have the ability to mutate to highly pathogenic avian influenza viruses (HPAIV) in poultry and therefore are the etiological agents of notifiable AIV (NAIV). It is of g...

journal_title：Journal of clinical microbiology

authors： Leijon M,Ullman K,Thyselius S,Zohari S,Pedersen JC,Hanna A,Mahmood S,Banks J,Slomka MJ,Belák S

更新日期：2011-11-01 00:00:00

abstract：:The BACTEC MGIT 960 system, a fully automated, nonradiometric, noninvasive system for detection and drug susceptibility testing of mycobacteria, was evaluated for the ability to test susceptibilities to second-line drugs. In this study, which was carried out in three phases (phase I, mostly susceptible strains; phase ...

journal_title：Journal of clinical microbiology

authors： Rüsch-Gerdes S,Pfyffer GE,Casal M,Chadwick M,Siddiqi S

更新日期：2006-03-01 00:00:00

abstract：:A simple new agar medium containing L-canavanine, glycine, and bromthymol blue was found to give a clearer and more accurate distinction between serotype A or D (Cryptococcus neoformans var. neoformans) and serotype B or C (C. neoformans var. gattii) than creatinine-dextrose-bromthymol blue or glycine-cycloheximide-ph...

journal_title：Journal of clinical microbiology

authors： Kwon-Chung KJ,Polacheck I,Bennett JE

更新日期：1982-03-01 00:00:00

abstract：:Decreasing the time to species identification and antibiotic susceptibility determination of strains recovered from patients with bacteremia significantly decreases morbidity and mortality. Herein, we validated a method to identify Gram-negative bacteria directly from positive blood culture medium using the Bruker MAL...

journal_title：Journal of clinical microbiology

authors： Wimmer JL,Long SW,Cernoch P,Land GA,Davis JR,Musser JM,Olsen RJ

更新日期：2012-07-01 00:00:00

abstract：:Eleven reference and 25 clinical isolates of Fusarium were subject to multilocus DNA sequence analysis to determine the species and haplotypes of the fusarial isolates from Beijing and Shandong, China. Seven loci were analyzed: the translation elongation factor 1 alpha gene (EF-1α); the nuclear rRNA internal transcrib...

journal_title：Journal of clinical microbiology

authors： Wang H,Xiao M,Kong F,Chen S,Dou HT,Sorrell T,Li RY,Xu YC

更新日期：2011-05-01 00:00:00

abstract：:Acinetobacter spp. are important nosocomial pathogens reported with increasing frequency in outbreaks of cross-infection during the past 2 decades. The majority of such outbreaks are caused by Acinetobacter baumannii. To investigate whether desiccation tolerance may be involved in the ability of certain strains of A. ...

journal_title：Journal of clinical microbiology

authors： Jawad A,Seifert H,Snelling AM,Heritage J,Hawkey PM

更新日期：1998-07-01 00:00:00

abstract：:In a prospective study of 16 patients with Legionnaires disease confirmed by cultural isolation of Legionella pneumophila from the respiratory tract, 38% (6 of 16) had positive blood cultures. Daily subcultures were made onto buffered charcoal-yeast extract plates from 6B aerobic and 7C anaerobic BACTEC blood culture ...

journal_title：Journal of clinical microbiology

authors： Rihs JD,Yu VL,Zuravleff JJ,Goetz A,Muder RR

更新日期：1985-09-01 00:00:00

abstract：:Hepatitis C virus (HCV) infection is an increasing health problem worldwide. Quantitative assays for HCV viral load are valuable in predicting response to therapy and for following treatment efficacy. Unfortunately, most quantitative tests for HCV RNA are limited by poor sensitivity. We have developed a convenient, hi...

journal_title：Journal of clinical microbiology

authors： Cook L,Ng KW,Bagabag A,Corey L,Jerome KR

更新日期：2004-09-01 00:00:00

abstract：:A large pool of refined endotoxin was prepared from Escherichia coli O113 by extraction with hot acqueous phenol. It was characterized chemically and biologically and will be available for a reference standard designated as reference endotoxin EC. ...

journal_title：Journal of clinical microbiology

authors： Rudbach JA,Akiya FI,Elin RJ,Hochstein HD,Luoma MK,Milner EC,Milner KC,Thomas KR

更新日期：1976-01-01 00:00:00

abstract：:Chikungunya fever is a mosquito-borne disease of key public health importance in tropical and subtropical countries. Although severe joint pain is the most distinguishing feature of chikungunya fever, diagnosis remains difficult because the symptoms of chikungunya fever are shared by many pathogens, including dengue f...

journal_title：Journal of clinical microbiology

authors： Okabayashi T,Sasaki T,Masrinoul P,Chantawat N,Yoksan S,Nitatpattana N,Chusri S,Morales Vargas RE,Grandadam M,Brey PT,Soegijanto S,Mulyantno KC,Churrotin S,Kotaki T,Faye O,Faye O,Sow A,Sall AA,Puiprom O,Chaichana P,

更新日期：2015-02-01 00:00:00

abstract：:The technique of random amplified polymorphic DNA (RAPD) was adapted and optimized to study Haemophilus ducreyi isolates. A panel of 43 strains isolated from chancroid patients from different countries in Africa, Europe, North America, and Asia were characterized. The strains were also studied with respect to lipoolig...

journal_title：Journal of clinical microbiology

authors： Mbwana J,Bölin I,Lyamuya E,Mhalu F,Lagergård T

更新日期：2006-01-01 00:00:00

abstract：:A high-performance liquid chromatography method that utilized fluorescence detection (HPLC-FL) of mycolic acid 6,7-dimethoxycoumarin esters was developed to identify Mycobacterium tuberculosis (MTB) and M. avium complex (MAC) directly from fluorochrome stain smear-positive sputum specimens and young BACTEC 12B culture...

journal_title：Journal of clinical microbiology

authors： Jost KC Jr,Dunbar DF,Barth SS,Headley VL,Elliott LB

更新日期：1995-05-01 00:00:00

abstract：:The biochemical characteristics and the presence of human Fc(gamma) receptors of 52 throat isolates of group C beta-hemolytic streptococci were examined. Among these isolates, 38 were identified as "Streptococcus milleri" and 14 were identified as "Streptococcus equisimilis." The differentiation of group C "S. equisim...

journal_title：Journal of clinical microbiology

authors： Lebrun L,Guibert M,Wallet P,de Maneville MM,Pillot J

更新日期：1986-11-01 00:00:00

abstract：:Amplification of human papillomavirus (HPV) DNA by L1 consensus primer systems (e.g., MY09/11 or GP5(+)/6(+)) can detect as few as 10 to 100 molecules of HPV targets from a genital sample. However, genotype determination by dot blot hybridization is laborious and requires at least 27 separate hybridizations for substa...

journal_title：Journal of clinical microbiology

authors： Gravitt PE,Peyton CL,Apple RJ,Wheeler CM

更新日期：1998-10-01 00:00:00

abstract：:Although Escherichia fergusonii has been identified for decades, it has rarely been recovered from clinical specimens and its clinical significance remains unclear. We describe a case of E. fergusonii bacteremia in a diabetic patient with pancreatic cancer. The isolate was confirmed by three commercial identification ...

journal_title：Journal of clinical microbiology

authors： Lai CC,Cheng A,Huang YT,Chung KP,Lee MR,Liao CH,Hsueh PR

更新日期：2011-11-01 00:00:00

abstract：:We have recently developed a PCR assay for detection of Mycobacterium spp. at the genus level based on the Cobas Amplicor platform. The sensitivities for smear-positive and smear-negative specimens were found to be 100% and 47.9%, respectively. The specificity was 97.7%, the positive predictive value 84.6%, and the ne...

journal_title：Journal of clinical microbiology

authors： Peter-Getzlaff S,Lüthy J,Voit A,Bloemberg GV,Böttger EC

更新日期：2010-11-01 00:00:00

abstract：:The radioimmunoprecipitation assay (RIPA) has been used as a confirmatory test in several ongoing and published studies of Trypanosoma cruzi in blood donors in the United States. Despite its use as a confirmatory test, few studies are available comparing RIPA to commercially available serologic test methods. Thus, we ...

journal_title：Journal of clinical microbiology

authors： Leiby DA,Wendel S,Takaoka DT,Fachini RM,Oliveira LC,Tibbals MA

更新日期：2000-02-01 00:00:00

abstract：:We report an uncommon clinical presentation of a unique case of fatal invasive fungal cerebral vasculitis due to Arthrographis kalrae in a nonimmunocompromised host. The identity of the fungus was determined by morphological characteristics and by analysis of internal transcribed spacer 1 sequences and was confirmed b...

journal_title：Journal of clinical microbiology

authors： Pichon N,Ajzenberg D,Desnos-Ollivier M,Clavel M,Gantier JC,Labrousse F

更新日期：2008-09-01 00:00:00