Rapid PCR-based molecular pathotyping of H5 and H7 avian influenza viruses.

abstract：:We have used comparative genomic hybridization (CGH) on a full-genome Campylobacter jejuni microarray to examine genome-wide gene conservation patterns among 51 strains isolated from food and clinical sources. These data have been integrated with data from three previous C. jejuni CGH studies to perform a meta-analysi...

journal_title：Journal of clinical microbiology

authors： Taboada EN,Acedillo RR,Carrillo CD,Findlay WA,Medeiros DT,Mykytczuk OL,Roberts MJ,Valencia CA,Farber JM,Nash JH

更新日期：2004-10-01 00:00:00

abstract：:The production and characterization of five monoclonal antibodies to Mycobacterium tuberculosis are described. Specificity of the monoclonal antibodies was tested against other mycobacterial species by an enzyme-linked immunosorbent assay and immunoblots. HGT 3a, an immunoglobulin M (IgM) antibody, recognizes a molecu...

journal_title：Journal of clinical microbiology

authors： Kadival GV,Chaparas SD

更新日期：1987-01-01 00:00:00

abstract：:Real-time PCR shows the widespread presence of Coxiella burnetii DNA in a broad range of commercially available milk and milk products. MLVA genotyping shows that this is the result of the presence of a predominant C. burnetii genotype in the dairy cattle population. ...

journal_title：Journal of clinical microbiology

authors： Tilburg JJ,Roest HJ,Nabuurs-Franssen MH,Horrevorts AM,Klaassen CH

更新日期：2012-06-01 00:00:00

abstract：:In total, 521 nonrespiratory specimens (91 urine, 30 gastric aspirate, 245 tissue, 113 pleural fluid, 19 cerebrospinal fluid [CSF], and 23 stool specimens) submitted to the German National Reference Laboratory for Mycobacteria (NRL) from May 2009 to August 2010 were comparatively investigated with the new molecular-ba...

journal_title：Journal of clinical microbiology

authors： Hillemann D,Rüsch-Gerdes S,Boehme C,Richter E

更新日期：2011-04-01 00:00:00

abstract：:The effect of supplementing nutrient substrate with various combinations of concentrations of oleic and linoleic acids on the growth of 11 strains of Staphylococcus aureus was assessed. Whereas increasing the concentration of linoleic acid by itself greatly diminished the growth of all 11 strains, concomitant increase...

journal_title：Journal of clinical microbiology

authors： Campbell IM,Crozier DN,Pawagi AB,Buivids IA

更新日期：1983-08-01 00:00:00

abstract：:An investigation was made on the effect of acetone fixation on infectivity and immunofluorescent antigenicity of respiratory syncytial virus and adenovirus type 5, lipid- and nonlipid-containing viruses, respectively. Viruses were allowed to replicate in HEp-2 cells, and the cells were then fixed in acetone at 5 C for...

journal_title：Journal of clinical microbiology

authors： Bardell D

更新日期：1975-02-01 00:00:00

abstract：:A nationwide study on candidemia was conducted in Iceland from 1980 to 1999. The annual incidence increased from 1.4 cases/100,000 inhabitants/year between 1980 and 1984 to 4.9 cases/100,000 inhabitants/year between 1995 and 1999 (P < 0.0001). Candidemia episodes at university hospitals increased from 0.15/1,000 admis...

journal_title：Journal of clinical microbiology

authors： Asmundsdóttir LR,Erlendsdóttir H,Gottfredsson M

更新日期：2002-09-01 00:00:00

abstract：:A typing enzyme immunoassay (TYPE-EIA) was used to determine the antigenic types of 64 astrovirus-positive specimens from nine collections from seven countries. Six of the seven known astrovirus types were detected in the collections, with HAstV-1 predominating in all collections for one from the United Kingdom. Selec...

journal_title：Journal of clinical microbiology

authors： Noel JS,Lee TW,Kurtz JB,Glass RI,Monroe SS

更新日期：1995-04-01 00:00:00

abstract：:We are reporting on a nosocomial outbreak of 213 cases of vancomycin-resistant enterococcus infection involving 2,812 enterococcal isolates from patients over a period of 36 months. In 1990, the Enterococcus faecium vancomycin susceptibility rate was found to be 85.7% (36 of 42 cases), and an incidence of 10.9% (42 of...

journal_title：Journal of clinical microbiology

authors： Boyle JF,Soumakis SA,Rendo A,Herrington JA,Gianarkis DG,Thurberg BE,Painter BG

更新日期：1993-05-01 00:00:00

abstract：:Large numbers of Mycobacterium tuberculosis isolates that were obtained from patients' sputa on diagnosis and during follow-up after short-course chemotherapy in Madras, India, have either no copy or only a single copy of IS6110. This poses a limitation for DNA fingerprinting with an IS6110-based probe to determine th...

journal_title：Journal of clinical microbiology

authors： Sahadevan R,Narayanan S,Paramasivan CN,Prabhakar R,Narayanan PR

更新日期：1995-11-01 00:00:00

abstract：:This study assessed the diagnostic performance of the new COVID19SEROSpeed IgM/IgG rapid test (BioSpeedia, a spinoff of the Pasteur Institute of Paris) for the detection of antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in comparison to other commercial antibody assays through a large ...

journal_title：Journal of clinical microbiology

authors： Plebani M,Parčina M,Bechri I,Zehender G,Terkeš V,Abdel Hafith B,Antinori S,Pillet S,Gonzalo S,Hoerauf A,Lai A,Morović M,Bourlet T,Torre A,Pozzetto B,Galli M

更新日期：2021-01-21 00:00:00

abstract：:To examine the validity of cultures of fluid collected through drainage catheters, we reviewed retrospectively fluid specimens that had been collected through catheters in place for at least 2 days. These specimens were taken from patients at a large tertiary-care hospital. A total of 974 specimens representing 620 pa...

journal_title：Journal of clinical microbiology

authors： Everts RJ,Heneghan JP,Adholla PO,Reller LB

更新日期：2001-01-01 00:00:00

abstract：:We report the recovery of Histoplasma capsulatum from blood specimens cultured for Mycobacterium sp. in BACTEC 13A radiometric medium. H. capsulatum was recovered from six of eight blood specimens submitted for mycobacterial cultures from five human immunodeficiency virus-positive individuals. Initial positive metabol...

journal_title：Journal of clinical microbiology

authors： Merz WG,Kodsy S,Merz CS

更新日期：1992-01-01 00:00:00

abstract：:Recent studies have resulted in the proposal of a new species, Aeromonas schubertii (mannitol, sucrose, and indole negative), formerly termed Enteric Group 501, on the basis of the study of seven strains isolated from the southeastern and southwestern United States and Puerto Rico. We have isolated two phenotypically ...

journal_title：Journal of clinical microbiology

authors： Carnahan AM,Marii MA,Fanning GR,Pass MA,Joseph SW

更新日期：1989-08-01 00:00:00

abstract：:In our nursery, we identified neonates with positive urine latex particle agglutination (LPA) tests for group B streptococcus (GBS) antigen who did not have corroborating cultural evidence of infection. To investigate the mechanisms underlying these apparent false-positive reactions, we examined the urine LPA test in ...

journal_title：Journal of clinical microbiology

authors： Harris MC,Deuber C,Polin RA,Nachamkin I

更新日期：1989-10-01 00:00:00

abstract：:A 49-year-old woman with a 15-year history of HIV-hepatitis C virus coinfection had aortic valve and ascending aorta replacement in 2007. She presented with abdominal pain, episodic diarrhea, and profuse sweating in 2010. Thoracoabdominal positron emission tomography-computed tomography finally suggested infectious ao...

journal_title：Journal of clinical microbiology

authors： Rougemont M,Ratib O,Wintsch J,Schrenzel J,Hirschel B

更新日期：2013-08-01 00:00:00

abstract：:Two-hundred consecutive, single patient isolates of Enterobacter spp., Serratia spp., Citrobacter spp., and Pseudomonas aeruginosa were evaluated for AmpC production using a variety of inducer-substrate antibiotic combinations in a disk approximation format. The combinations examined included cefoxitin-piperacillin, i...

journal_title：Journal of clinical microbiology

authors： Dunne WM Jr,Hardin DJ

更新日期：2005-12-01 00:00:00

abstract：:To detect Streptococcus pneumoniae colonization, the nasopharynx is sampled using a swab placed in skim milk-tryptone-glucose-glycerol (STGG) transport medium, and then the swab specimen or STGG medium is cultured or subjected to PCR. We evaluated the effect of swab composition and compared the sensitivities of detect...

journal_title：Journal of clinical microbiology

authors： Rubin LG,Rizvi A,Baer A

更新日期：2008-08-01 00:00:00

abstract：:The mold Aureobasidium pullulans was isolated on several nutrient media from a splenic abscess in a patient with disseminated lymphoma. Examination of stained smears and paraffin sections revealed fungal structures characteristic of this organism. This is the first reported association of A. pullulans with an opportun...

journal_title：Journal of clinical microbiology

authors： Salkin IF,Martinez JA,Kemna ME

更新日期：1986-05-01 00:00:00

abstract：:Chlamydia pecorum is a significant pathogen of domestic livestock and wildlife. We have developed a C. pecorum-specific multilocus sequence analysis (MLSA) scheme to examine the genetic diversity of and relationships between Australian sheep, cattle, and koala isolates. An MLSA of seven concatenated housekeeping gene ...

journal_title：Journal of clinical microbiology

authors： Jelocnik M,Frentiu FD,Timms P,Polkinghorne A

更新日期：2013-08-01 00:00:00

abstract：:Specific precipitating and opsonic neutralizing antigens were identified in dilute-nitrous acid extracts of group A streptococci. This was done by decreasing the temperature and time of exposure of the streptococcal cells to the nitrous acid. Ninety percent of 31 strains of group A streptococcal dilute-nitrous acid ex...

journal_title：Journal of clinical microbiology

authors： Hafez K,El Kholy AM,Facklam RR

更新日期：1981-11-01 00:00:00

abstract：:The purpose of this study was to evaluate the performance of a herpes simplex virus (HSV) type 1-specific anti-glycoprotein C-1 monoclonal antibody (MAb) and a type 2-specific anti-glycoprotein G-2 MAb for typing of 2,400 clinical HSV-1 isolates and 2,400 clinical HSV-2 isolates, respectively, using an enzyme immunoas...

journal_title：Journal of clinical microbiology

authors： Liljeqvist JA,Svennerholm B,Bergström T

更新日期：1999-08-01 00:00:00

abstract：:A real-time PCR assay was developed targeting the bla(KPC) responsible for Klebsiella pneumoniae carbapenemase (KPC)-mediated carbapenem resistance and was validated for testing colonies or enrichment broth cultures. The assay accurately detects KPC-containing strains with high analytical specificity and sensitivity. ...

journal_title：Journal of clinical microbiology

authors： Mangold KA,Santiano K,Broekman R,Krafft CA,Voss B,Wang V,Hacek DM,Usacheva EA,Thomson RB Jr,Kaul KL,Peterson LR

更新日期：2011-09-01 00:00:00

abstract：:Since January 1988, Staphylococcus aureus strains with a high level of quinolone resistance have been isolated at 17 hospitals and 15 nursing homes in New York City. The majority of these strains were methicillin resistant. The bacteriophage types and susceptibility to other antibiotics were similar to those of quinol...

journal_title：Journal of clinical microbiology

authors： Schaefler S

更新日期：1989-02-01 00:00:00

abstract：:The value of immunoglobulin M (IgM) detection in the early diagnosis of influenza A was examined in a prospective study during an outbreak of influenza A/Philippines/2/82 (H3N2) virus infection in February and March 1986. The IgM response was investigated in sera from 64 adults with influenzalike symptoms; we found a ...

journal_title：Journal of clinical microbiology

authors： Vikerfors T,Lindegren G,Grandien M,van der Logt J

更新日期：1989-03-01 00:00:00

abstract：:A growth medium with a specific oxidation-reduction potential containing peptone, dextrose, sodium succinate, sodium lactate, gelatin, sodium bicarbonate and blue tetrazolium, an indicator dye, in a tris(hydroxymethyl)aminomethane buffer was used to detect the presence of microorganisms in blood. The procedure involve...

journal_title：Journal of clinical microbiology

authors： Shih CN,Balish E

更新日期：1977-09-01 00:00:00

abstract：:The prevalence of Leishmania infantum-specific antibodies and asymptomatic infection was assessed in a randomized sample of 526 healthy adults from a continental area of northwestern Italy where L. infantum is not endemic and where autochthonous cases of visceral leishmaniasis (VL) were recently reported. L. infantum-...

journal_title：Journal of clinical microbiology

authors： Biglino A,Bolla C,Concialdi E,Trisciuoglio A,Romano A,Ferroglio E

更新日期：2010-01-01 00:00:00

abstract：:Sputum smear microscopy is an easy, inexpensive, and rapid method for detecting tubercle bacilli when there are more than 10,000 bacilli/ml in the original sputum. Furthermore, because the microscopic method provides not only quantitative, but also qualitative information, such as the shape of bacilli, it has remained...

journal_title：Journal of clinical microbiology

authors： Yamada H,Mitarai S,Wahyunitisari MR,Mertaniasih NM,Sugamoto T,Chikamatsu K,Aono A,Matsumoto H,Fujiki A

更新日期：2011-10-01 00:00:00

abstract：:We report the first case of a postoperative wound infection caused by Vibrio metschnikovii on the lower right leg of a patient after saphenectomy. Compared to the healing of an uninfected site, that of the right leg was delayed, and a cure was achieved by intensified wound care. Several swabs taken from the infected s...

journal_title：Journal of clinical microbiology

authors： Linde HJ,Kobuch R,Jayasinghe S,Reischl U,Lehn N,Kaulfuss S,Beutin L

更新日期：2004-10-01 00:00:00

abstract：:Twenty-nine Borrelia burgdorferi isolates, obtained from dusky-footed wood rats (Neotoma fuscipes) and California kangaroo rats (Dipodomys californicus) in California, were analyzed genetically. Chromosomal DNA was examined by restriction endonuclease analysis (REA) and gene probe restriction fragment length polymorph...

journal_title：Journal of clinical microbiology

authors： Zingg BC,Brown RN,Lane RS,LeFebvre RB

更新日期：1993-12-01 00:00:00