Identification of Mycobacterium tuberculosis and M. avium complex directly from smear-positive sputum specimens and BACTEC 12B cultures by high-performance liquid chromatography with fluorescence detection and computer-driven pattern recognition models.

abstract：:Fifty-six Pasteurella multocida strains (40 P. multocida subsp. septica and 16 P. multocida subsp. multocida strains) isolated from the mouths of 56 dogs among the 134 living in a French canine military training center (132e Groupe Cynophile de l'Armée de Terre, Suippes, France) were studied by use of enterobacterial ...

journal_title：Journal of clinical microbiology

authors： Loubinoux J,Lozniewski A,Lion C,Garin D,Weber M,Le Faou AE

更新日期：1999-08-01 00:00:00

abstract：:We document for the first time an infectious but nonarthritogenic variant of Borrelia burgdorferi. Strain 25015, previously isolated from an Ixodes dammini larva collected in upstate New York, was infectious but failed to produce arthritis or carditis in laboratory rats and mice. By contrast, pathogenic strain N40 inv...

journal_title：Journal of clinical microbiology

authors： Anderson JF,Barthold SW,Magnarelli LA

更新日期：1990-12-01 00:00:00

abstract：:Optimal conditions for demonstrating the presence of infectious human immunodeficiency virus in peripheral blood mononuclear cells (PMCs) from seropositive individuals involved cocultivation of infected cells with phytohemagglutinin-stimulated PMCs from seronegative donors in the presence of 2 micrograms of Polybrene ...

journal_title：Journal of clinical microbiology

authors： Castro BA,Weiss CD,Wiviott LD,Levy JA

更新日期：1988-11-01 00:00:00

abstract：:Patients with chronic granulomatous disease have a marked defect in neutrophil oxidative metabolism and microbicidal activity. Asymptomatic mothers of males with the disease can usually be identified as heterozygous carriers by intermediate leukocyte function. Most mothers of females with the disease, however, have no...

journal_title：Journal of clinical microbiology

authors： Mills EL,Rholl KS,Quie PG

更新日期：1980-07-01 00:00:00

abstract：:Legionella is the causative agent for Legionnaires' disease (LD) and is responsible for several large outbreaks in the world. More than 90% of LD cases are caused by Legionella pneumophila, and studies on the origin and transmission routes of this pathogen rely on adequate molecular characterization of isolates. Curre...

journal_title：Journal of clinical microbiology

authors： Bosch T,Euser SM,Landman F,Bruin JP,IJzerman EP,den Boer JW,Schouls LM

更新日期：2015-10-01 00:00:00

abstract：:Detection of human cytomegalovirus (CMV) DNA in clinical specimens is considered a cornerstone in the diagnosis of CMV disease. The aim of this study was to evaluate a newly designed LightCycler-based quantitative CMV PCR. Specimens of human origin (n = 200) were tested using the LightCycler PCR, the quantitative COBA...

journal_title：Journal of clinical microbiology

authors： Schaade L,Kockelkorn P,Ritter K,Kleines M

更新日期：2000-11-01 00:00:00

abstract：:Helicobacter cetorum sp. nov. has been cultured from the stomach of Atlantic white-sided dolphins (Lagenorhynchus acutus) and the feces of Pacific white-sided (L. obliquidens) and Atlantic bottlenose (Tursiops truncatus) dolphins and a beluga whale (Delphinapterus leucas). H. cetorum has high homology to Helicobacter ...

journal_title：Journal of clinical microbiology

authors： Harper CG,Whary MT,Feng Y,Rhinehart HL,Wells RS,Xu S,Taylor NS,Fox JG

更新日期：2003-07-01 00:00:00

abstract：:The objective of this study was to measure the performance of the Affirm Ambient Temperature Transport System (ATTS) over time and to estimate the length of time the system can preserve a vaginal specimen containing the three common organisms causing vaginitis: Trichomonas vaginalis, Candida species, and Gardnerella v...

journal_title：Journal of clinical microbiology

authors： Brown HL,Fuller DA,Davis TE,Schwebke JR,Hillier SL

更新日期：2001-09-01 00:00:00

abstract：:Accurate and timely diagnosis of dengue virus (DEN) infections is essential for the differential diagnosis of patients with febrile illness and hemorrhagic fever. In the present study, the diagnostic value of a newly developed immune-complex dissociated nonstructural-1 (NS-1) antigen dot blot immunoassay (DBI) was com...

journal_title：Journal of clinical microbiology

authors： Koraka P,Burghoorn-Maas CP,Falconar A,Setiati TE,Djamiatun K,Groen J,Osterhaus AD

更新日期：2003-09-01 00:00:00

abstract：:We implemented hospital information system (HIS) alerts to deter unnecessary test orders for ovum and parasite (O&P) exams and Clostridium difficile PCR. The HIS alerts decreased noncompliant O&P orders (orders after >72 h of hospitalization) from 49.8% to 30.9%, an overall decrease of 19%, and reduced noncompliant C....

journal_title：Journal of clinical microbiology

authors： Otto CC,Shuptar SL,Milord P,Essick CJ,Nevrekar R,Granovsky SL,Seo SK,Babady NE,Martin SC,Tang YW,Pessin MS

更新日期：2015-08-01 00:00:00

abstract：:Total DNA from five Campylobacter species was digested with a mixture of XhoI and BglII restriction endonucleases and analyzed by Southern hybridization by using a probe complementary to the DNA coding for the 16S rRNA. Each of the Campylobacter species, including C. jejuni, C. coli, C. laridis, C. fetus, and C. upsal...

journal_title：Journal of clinical microbiology

authors： Moureau P,Derclaye I,Gregoire D,Janssen M,Cornelis GR

更新日期：1989-07-01 00:00:00

abstract：:A total of 49 adenovirus type 3 (Ad3) strains from patients with epidemic keratoconjunctivitis at an eye clinic in Sapporo, Japan, from 1983 to 1986 were classified into either genome type Ad3f (26 strains) or Ad3g (23 strains). By the use of Hinf1 and TaqI restriction endonucleases, they were classified into 11 and 3...

journal_title：Journal of clinical microbiology

authors： Itakura S,Aoki K,Sawada H,Shinagawa M

更新日期：1990-10-01 00:00:00

abstract：:Blastomyces dermatitidis and Histoplasma capsulatum are dimorphic fungi that often cause self-limited respiratory infections. However, they may also cause severe disseminated disease, depending on the level of the exposure to the organism and the host immune status. In addition, patients with infections caused by thes...

journal_title：Journal of clinical microbiology

authors： Babady NE,Buckwalter SP,Hall L,Le Febre KM,Binnicker MJ,Wengenack NL

更新日期：2011-09-01 00:00:00

abstract：:Monkey pox virus was mechanically disrupted by low temperature and high pressure into soluble and insoluble fractions. Soluble fractions elicited virus-neutralizing antibodies (1:20 to 1:160) in rabbits, whereas the insoluble (in saline) fractions did not (less than 1:5). No infectious virus was detected after the dis...

journal_title：Journal of clinical microbiology

authors： Olsen RG,Blakeslee JR,Mathes L,Nakano JH

更新日期：1977-07-01 00:00:00

abstract：:The 30-micrograms cefprozil disk and the cephalosporin class (30-micrograms cephalothin) disk were compared for their abilities to predict cefprozil susceptibility by agar disk diffusion testing. High error (5.02% major and 14.11% minor) rates were encountered with the cephalothin disk and were most frequently observe...

journal_title：Journal of clinical microbiology

authors： Fung-Tomc J,Stickle T,Doyle C,Huczko E,Kessler RE

更新日期：1991-11-01 00:00:00

abstract：:A total of 221 isolates of multidrug-resistant Salmonella enterica serovar Typhimurium in Japan were characterized in the present study. The results revealed that clonal serovar Typhimurium definitive phage type 104 strains prevailed and that these strains had drug resistance patterns, integron types, and pulsed-field...

journal_title：Journal of clinical microbiology

authors： Izumiya H,Terajima J,Matsushita S,Tamura K,Watanabe H

更新日期：2001-07-01 00:00:00

abstract：:Lipopolysaccharide components 3-deoxy-D-manno-2-octulosonic acid and L-glycero-D-manno-heptose were detected in hydrolysates from whole cells of Neisseria elongata by gas-liquid chromatography. Cells from a single plate were hydrolyzed, and carbohydrate components were converted to aldononitrile and O-methyloxime acet...

journal_title：Journal of clinical microbiology

authors： Guerrant GO,Moss CW

更新日期：1986-03-01 00:00:00

abstract：:Candida famata (teleomorph Debaryomyces hansenii) has been described as a medically relevant yeast, and this species has been included in many commercial identification systems that are currently used in clinical laboratories. Among 53 strains collected during the SENTRY and ARTEMIS surveillance programs and previousl...

journal_title：Journal of clinical microbiology

authors： Castanheira M,Woosley LN,Diekema DJ,Jones RN,Pfaller MA

更新日期：2013-01-01 00:00:00

abstract：:Rapid tests for glucose, maltose, lactose, sucrose, and starch fermentation were performed on 112 strains of anaerobic bacteria. The tests were incubated under aerobic conditions, and results were read within 4 h. An overall correlation of 89% was achieved between the rapid tests and the Virginia Polytechnic Institut...

journal_title：Journal of clinical microbiology

authors： Schreckenberger PC,Blazevic DJ

更新日期：1976-03-01 00:00:00

abstract：:A total of 30 isolates of Treponema hyodysenteriae collected in the Saint-Hyacinthe (Quebec, Canada) area were serotyped by agar gel double immunodiffusion by using extracted lipopolysaccharide and hyperimmune rabbit antisera. Only 17% (5 of 30) of the isolates were typed with antisera specific for each of the seven k...

journal_title：Journal of clinical microbiology

authors： Li ZS,Bélanger M,Jacques M

更新日期：1991-12-01 00:00:00

abstract：:Stool microscopy as performed in clinical parasitology laboratories is a complex procedure with subjective interpretation. Quality assurance (QA) programs often emphasize proficiency testing as an assessment tool. We describe a result reproducibility assessment tool, which can form part of a broader QA program, and wh...

journal_title：Journal of clinical microbiology

authors： Libman MD,Gyorkos TW,Kokoskin E,Maclean JD

更新日期：2008-07-01 00:00:00

abstract：:The API Staph-Ident system was evaluated as a means for identifying the species of bovine strains of staphylococci routinely isolated from quarter-milk samples. The species identity of 314 of 581 (54%) isolates of staphylococci was correctly determined by this method. The API Staph-Ident system was more accurate in co...

journal_title：Journal of clinical microbiology

authors： Langlois BE,Harmon RJ,Akers K

更新日期：1983-11-01 00:00:00

abstract：:Eleven isolates of Aeromonas species from human stool cultures were found to produce enterotoxin activity as determined by assay of culture filtrates in rabbit intestinal loops and rabbit skin and on adrenal Y1 cells. Hemolysin(s) and a cytotoxic protein were found to interfere in all three assay systems but could be ...

journal_title：Journal of clinical microbiology

authors： Ljungh A,Popoff M,Wadstrom T

更新日期：1977-08-01 00:00:00

abstract：:Bacteroides forsythus has been associated with destructive adult periodontitis. Up to now, detailed analysis by classical means was hampered by the fastidious nature of the organism. There is hope that the application of molecular detection methods such as indirect immunofluorescence or in situ hybridization (ISH) wil...

journal_title：Journal of clinical microbiology

authors： Gersdorf H,Meissner A,Pelz K,Krekeler G,Göbel UB

更新日期：1993-04-01 00:00:00

abstract：:We report a case of staphylococcal exanthematous disease in a newborn due to a toxic shock syndrome toxin 1- and SEC-producing methicillin-resistant Staphylococcus aureus strain and alert neonatologists to the probable emergence in France of the neonatal toxic shock syndrome-like exanthematous disease in newborns prev...

journal_title：Journal of clinical microbiology

authors： van der Mee-Marquet N,Lina G,Quentin R,Yaouanc-Lapalle H,Fièvre C,Takahashi N,Etienne J

更新日期：2003-10-01 00:00:00

abstract：:Five strains of an unknown, multidrug-resistant coryneform, gram-positive rod were isolated from blood, bronchial aspirate, and abscess specimens. Four of the five strains isolated were highly resistant to antimicrobial agents, including beta-lactams, aminoglycosides, macrolides, quinolones, and tetracyclines, except ...

journal_title：Journal of clinical microbiology

authors： Otsuka Y,Kawamura Y,Koyama T,Iihara H,Ohkusu K,Ezaki T

更新日期：2005-08-01 00:00:00

abstract：:Two multiplex PCR enzyme immunoassays (PCR-EIAs) were developed for Staphylococcus aureus exotoxin gene screening as an alternative to the conventional biological assays, which depend on detectable amounts of toxins produced. One set of oligonucleotide primers and probes was designed to search for enterotoxin A to E g...

journal_title：Journal of clinical microbiology

authors： Becker K,Roth R,Peters G

更新日期：1998-09-01 00:00:00

abstract：:The aims of this study were to evaluate the use of molecular fingerprinting for assessment of bacterial diversity in canine duodenal juice and to evaluate the variation in the small intestinal microflora at repeated sampling. Two groups of dogs were used. Duodenal juice was collected from eight dogs euthanized for an ...

journal_title：Journal of clinical microbiology

authors： Suchodolski JS,Ruaux CG,Steiner JM,Fetz K,Williams DA

更新日期：2004-10-01 00:00:00

abstract：:Ninety-one isolates of nutritionally variant streptococci (NVS) that were previously isolated from the human mouth were regarded as consisting of 7 Streptococcus defectivus isolates, 78 Streptococcus adjacens isolates, and 6 Gemella morbillorum isolates. However, recent references to the taxonomic reclassification of ...

journal_title：Journal of clinical microbiology

authors： Sato S,Kanamoto T,Inoue M

更新日期：1999-08-01 00:00:00

abstract：:Malassezia yeasts are associated with several dermatological disorders. The conventional identification of Malassezia species by phenotypic methods is complicated and time-consuming, and the results based on culture methods are difficult to interpret. A comparative molecular approach based on the use of three molecula...

journal_title：Journal of clinical microbiology

authors： Gupta AK,Boekhout T,Theelen B,Summerbell R,Batra R

更新日期：2004-09-01 00:00:00