Reducing Unnecessary and Duplicate Ordering for Ovum and Parasite Examinations and Clostridium difficile PCR in Immunocompromised Patients by Using an Alert at the Time of Request in the Order Management System.

abstract：:Rapid identification and differentiation of orthopoxviruses by PCR were achieved with primers based on genome sequences encoding the hemagglutinin (HA) protein, an infected-cell membrane antigen that distinguishes orthopoxviruses from other poxvirus genera. The initial identification step used a primer pair of consens...

journal_title：Journal of clinical microbiology

authors： Ropp SL,Jin Q,Knight JC,Massung RF,Esposito JJ

更新日期：1995-08-01 00:00:00

abstract：:To detect Streptococcus pneumoniae colonization, the nasopharynx is sampled using a swab placed in skim milk-tryptone-glucose-glycerol (STGG) transport medium, and then the swab specimen or STGG medium is cultured or subjected to PCR. We evaluated the effect of swab composition and compared the sensitivities of detect...

journal_title：Journal of clinical microbiology

authors： Rubin LG,Rizvi A,Baer A

更新日期：2008-08-01 00:00:00

abstract：:Twenty-four isolates of Actinobacillus seminis were typed by PCR ribotyping, repetitive extragenic palindromic element (REP)-based PCR, and enterobacterial repetitive intergenic consensus (ERIC)-based PCR. Five types were distinguished by REP-PCR, and nine types were distinguished by ERIC-PCR. PCR ribotyping produced ...

journal_title：Journal of clinical microbiology

authors： Appuhamy S,Coote JG,Low JC,Parton R

更新日期：1998-03-01 00:00:00

abstract：:Candida parapsilosis, a pathogenic yeast, is composed of three newly designated genomic species that are physiologically and morphologically indistinguishable. Nosocomial infections caused by group I C. parapsilosis are often associated with the breakdown of infection control practices and the contamination of medical...

journal_title：Journal of clinical microbiology

authors： Lasker BA,Butler G,Lott TJ

更新日期：2006-03-01 00:00:00

abstract：:A 24-year-old man sustained a crush injury to the tibia, which subsequently became infected with Saksenaea vasiformis. He was treated with debridement and a free myocutaneous flap, but amputation was necessary because of mycotic osteomyelitis. S. vasiformis was recovered and identified on the basis of its characterist...

journal_title：Journal of clinical microbiology

authors： Pierce PF,Wood MB,Roberts GD,Fitzgerald RH Jr,Robertson C,Edson RS

更新日期：1987-05-01 00:00:00

abstract：:An optimized reverse transcription (RT)-PCR protocol for the intravitam detection of rabies virus genomic RNA was tested with clinical samples obtained from 28 patients suspected of having rabies, 9 of whom were confirmed to have had rabies by postmortem examination. RT-PCR using saliva combined with an immunofluoresc...

journal_title：Journal of clinical microbiology

authors： Crepin P,Audry L,Rotivel Y,Gacoin A,Caroff C,Bourhy H

更新日期：1998-04-01 00:00:00

abstract：:Mycoplasma bovis is a major bovine pathogen associated with bovine respiratory disease complex and is responsible for substantial economic losses worldwide. M. bovis is also associated with other clinical presentations in cattle, including mastitis, otitis, arthritis, and reproductive disorders. To gain a better under...

journal_title：Journal of clinical microbiology

authors： Rosales RS,Churchward CP,Schnee C,Sachse K,Lysnyansky I,Catania S,Iob L,Ayling RD,Nicholas RA

更新日期：2015-03-01 00:00:00

abstract：:Pregnant cows were successfully hyperimmunized with all four human rotavirus serotypes, resulting in a 100-fold increase in neutralizing milk antibody titers over those of controls. Milk antibodies were isolated batchwise from 1,000 kg of pooled milk for the first 10 lactation days, yielding 10 kg of freeze-dried milk...

journal_title：Journal of clinical microbiology

authors： Brüssow H,Hilpert H,Walther I,Sidoti J,Mietens C,Bachmann P

更新日期：1987-06-01 00:00:00

abstract：:The reliability of an rRNA-derived oligonucleotide probe for Neisseria gonorrhoeae was tested with 187 N. gonorrhoeae isolates, 81 Neisseria meningitidis isolates, and several strains of other bacterial species. The probe proved to be 100% specific and 100% sensitive. N. gonorrhoeae cells could also be reliably identi...

journal_title：Journal of clinical microbiology

authors： Rossau R,Duhamel M,Van Dyck E,Piot P,Van Heuverswyn H

更新日期：1990-05-01 00:00:00

abstract：:In the treatment of serious bacterial infections, the rapid institution of appropriate antimicrobial chemotherapy may be lifesaving. Choosing the correct antibiotic or combination of antibiotics is becoming very important, as multidrug resistance is found in many pathogens. Using a collection of 75 well-characterized ...

journal_title：Journal of clinical microbiology

authors： Hujer KM,Hujer AM,Endimiani A,Thomson JM,Adams MD,Goglin K,Rather PN,Pennella TT,Massire C,Eshoo MW,Sampath R,Blyn LB,Ecker DJ,Bonomo RA

更新日期：2009-05-01 00:00:00

abstract：:Potomac horse fever is an acute systemic equine disease caused by Ehrlichia risticii. Currently, serologic methods are widely used to diagnose this disease. However, serologic methods cannot determine whether the horse is presently infected or has been exposed to ehrlichial antigens in the past. The purpose of the pre...

journal_title：Journal of clinical microbiology

authors： Mott J,Rikihisa Y,Zhang Y,Reed SM,Yu CY

更新日期：1997-09-01 00:00:00

abstract：:Using the established commercial system Sherlock (MIDI, Inc.), cellular fatty acid methyl ester analysis for differentiation among Burkholderia cepacia complex species was proven. The identification key based on the diagnostic fatty acids is able to discern phenotypically related Ralstonia pickettii and Pandoraea spp....

journal_title：Journal of clinical microbiology

authors： Krejcí E,Kroppenstedt RM

更新日期：2006-03-01 00:00:00

abstract：:The usefulness of randomly amplified polymorphic DNA method (RAPD) to identify each species of genus Erysipelothrix and for epidemiological analysis of this genus was studied. Eighty-one strains and 18 random primers were tested. Among the tested primers, the primers NK51 (GGTGGTGGTATC) and NK6 (CCCGCGCCCC) produced n...

journal_title：Journal of clinical microbiology

authors： Okatani AT,Hayashidani H,Takahashi T,Taniguchi T,Ogawa M,Kaneko K

更新日期：2000-12-01 00:00:00

abstract：:We report a novel IS711 chromosomal location that is specific for the Brucella genotype ST27 previously associated with Pacific marine mammals and human zoonotic infection in New Zealand and Peru. Our data support the previous observation that this peculiar genotype is distinct from those commonly isolated from the At...

journal_title：Journal of clinical microbiology

authors： Cloeckaert A,Bernardet N,Koylass MS,Whatmore AM,Zygmunt MS

更新日期：2011-11-01 00:00:00

abstract：:Leifsonia aquatica is an aquatic bacterium that is typically found in environmental water habitats. Infections due to L. aquatica are rare and commonly catheter associated in immunocompromised patients. We report the first case of an acute septicemia caused by L. aquatica in a healthy immunocompetent host after cryope...

journal_title：Journal of clinical microbiology

authors： Han L,Lei JE,Wang X,Guo LT,Kang QY,He L,Xu JR

更新日期：2013-11-01 00:00:00

abstract：:Haemophilus influenzae type e biotype 4 was isolated from a single antemortem blood culture obtained from a 60-year-old, white male with abdominal carcinomatosis. ...

journal_title：Journal of clinical microbiology

authors： Cawthern TH,Marraro RV,McCleskey FK

更新日期：1978-01-01 00:00:00

abstract：:Laboratory and clinical diagnostic classification of seropositive individuals, followed by treatment and supportive therapy, is an established component of Chagas' disease control in areas where this disease is endemic. However, most Chagas' disease patients live in remote areas where neither equipped laboratories nor...

journal_title：Journal of clinical microbiology

authors： Roddy P,Goiri J,Flevaud L,Palma PP,Morote S,Lima N,Villa L,Torrico F,Albajar-Viñas P

更新日期：2008-06-01 00:00:00

abstract：:A prospective study of Campylobacter jejuni infection was performed during a 6-month period in a cohort of 198 Chilean infants. Surveillance was based on biweekly home visits by a team of trained nurses. C. jejuni colonization was studied by culturing stool samples from diarrheal episodes and paired asymptomatic contr...

journal_title：Journal of clinical microbiology

authors： Figueroa G,Galeno H,Troncoso M,Toledo S,Soto V

更新日期：1989-05-01 00:00:00

abstract：:Of the media examined, thioglycolate broth supplemented with 5% lysed sheep blood, Butzler antibiotic mixture, and 0.1% lauryl sulfate was the most sensitive enrichment medium for recovery of wild-type strains of Campylobacter jejuni from cecal contents of chickens and chicken livers. It allowed the retrieval of 1 CFU...

journal_title：Journal of clinical microbiology

authors： Barot MS,Bokkenheuser VD

更新日期：1984-07-01 00:00:00

abstract：:The epidemiology of 278 strains of Salmonella typhimurium isolated from 1973 to 1981 from animals in New York State was studied by using four "fingerprinting" techniques, bacteriophage type (B.R. Callow, J. Hyg. 57:346-359, 1959), biotype (J. P. Duguid, E. S. Anderson, G. A. Alfredsson, R. Barker, and D. C. Old, J. Me...

journal_title：Journal of clinical microbiology

authors： McDonough PL,Timoney JF,Jacobson RH,Khakhria R

更新日期：1989-04-01 00:00:00

abstract：:During an investigation of a hospital outbreak of norovirus gastroenteritis identified as being caused by a recombinant genogroup II (rGII-3a) strain, fecal specimens collected from asymptomatic staff and patients were tested by nested PCR. A GII-4 norovirus strain, the predominant strain associated with outbreaks in ...

journal_title：Journal of clinical microbiology

authors： Gallimore CI,Cubitt D,du Plessis N,Gray JJ

更新日期：2004-05-01 00:00:00

abstract：:The tick-borne rickettsia Cowdria ruminantium has been propagated continuously for over 500 days in the Ixodes scapularis tick cell line IDE8 by using the Gardel isolate from bovine endothelial cells as an inoculum. Infection of the tick cells was confirmed by PCR, karyotyping, electron microscopy, and reinfection of ...

journal_title：Journal of clinical microbiology

authors： Bell-Sakyi L,Paxton EA,Munderloh UG,Sumption KJ

更新日期：2000-03-01 00:00:00

abstract：:HIV-1 drug resistance (HIVDR) assays are important tools in clinical management of HIV-infected patients on antiretroviral therapy (ART) and surveillance of drug-resistant variants at population levels. The high cost associated with commercial assays hinders their use in resource-limited settings. We adopted and valid...

journal_title：Journal of clinical microbiology

authors： Inzaule S,Yang C,Kasembeli A,Nafisa L,Okonji J,Oyaro B,Lando R,Mills LA,Laserson K,Thomas T,Nkengasong J,Zeh C

更新日期：2013-02-01 00:00:00

abstract：:Acute and convalescent-phase sera obtained from 10 patients infected with a Japanese strain of spotted fever group (SFG) rickettsia were tested by the indirect immunoperoxidase test, the Weil-Felix test, an enzyme-linked immunosorbent assay (ELISA), and immunoblotting. By the Weil-Felix test, the reactivity of these s...

journal_title：Journal of clinical microbiology

authors： Amano K,Hatakeyama H,Okuta M,Suto T,Mahara F

更新日期：1992-09-01 00:00:00

abstract：:Capnocytophaga ochracea is a gram-negative, fusiform bacillus which is part of the normal human oral flora. This organism is often isolated from periodontal lesions of patients with periodontitis and is associated with sepsis in granulocytopenic patients. We report here a case of endocarditis caused by C. ochracea. ...

journal_title：Journal of clinical microbiology

authors： Buu-Hoi AY,Joundy S,Acar JF

更新日期：1988-05-01 00:00:00

abstract：:This report presents the case of a patient with a massive pyelonephritis and a urosepsis caused by Streptococcus pneumoniae. This case is unusual as the focus was distant from the respiratory tract, the usual primary site of infection caused by this organism. No other primary site of infection was documented. ...

journal_title：Journal of clinical microbiology

authors： Dufke S,Kunze-Kronawitter H,Schubert S

更新日期：2004-09-01 00:00:00

abstract：:Alpha-toxin is a major Staphylococcus aureus virulence factor. This study evaluated potential relationships between in vitro alpha-toxin expression of S. aureus bloodstream isolates, anti-alpha-toxin antibody in serum of patients with S. aureus bacteremia (SAB), and clinical outcomes in 100 hemodialysis and 100 postsu...

journal_title：Journal of clinical microbiology

authors： Sharma-Kuinkel BK,Wu Y,Tabor DE,Mok H,Sellman BR,Jenkins A,Yu L,Jafri HS,Rude TH,Ruffin F,Schell WA,Park LP,Yan Q,Thaden JT,Messina JA,Fowler VG Jr,Esser MT

更新日期：2015-01-01 00:00:00

abstract：:We report an uncommon clinical presentation of a unique case of fatal invasive fungal cerebral vasculitis due to Arthrographis kalrae in a nonimmunocompromised host. The identity of the fungus was determined by morphological characteristics and by analysis of internal transcribed spacer 1 sequences and was confirmed b...

journal_title：Journal of clinical microbiology

authors： Pichon N,Ajzenberg D,Desnos-Ollivier M,Clavel M,Gantier JC,Labrousse F

更新日期：2008-09-01 00:00:00

abstract：:The penicillin-induced L phase of growth of the group B, type III streptococcal strain 76-043 was examined for biochemical properties used for the identification of group B streptococci. After numerous serial subcultures in the cell wall-defective state, this stable L phase continued to produce hemolysin, hippuricase,...

journal_title：Journal of clinical microbiology

authors： Flores AE,Ferrieri P

更新日期：1983-10-01 00:00:00

abstract：:For prevention and control of gonorrhea, an objective, highly discriminating, and reproducible molecular epidemiological characterization of Neisseria gonorrhoeae is essential. In the present study, in pursuance of providing such qualities, pyrosequencing technology, a fast real-time DNA sequence analysis, was applied...

journal_title：Journal of clinical microbiology

authors： Unemo M,Olcén P,Jonasson J,Fredlund H

更新日期：2004-07-01 00:00:00