Bacteremia associated with Haemophilus influenzae type e biotype 4.

abstract：:New Delhi metallo-β-lactamase (NDM)-producing bacteria are considered potential global health threats. It is necessary to monitor NDM-1 and its variants in clinical isolates in order to understand the NDM-1 epidemic and the impact of its variants on β-lactam resistance. To reduce the lengthy time needed for cloning an...

journal_title：Journal of clinical microbiology

authors： Huang L,Hu X,Zhou M,Yang Y,Qiao J,Wang D,Yu J,Cui Z,Zhang Z,Zhang XE,Wei H

更新日期：2014-06-01 00:00:00

abstract：:Bartonella henselae uveitis was diagnosed in a 40-year-old woman with underlying HLA-B27 uveitis on the basis of immunodetection and molecular detection of the organism, a Marseilles genogroup and a CAL-1 genotype strain, in the vitreous fluid. This case illustrates that B. henselae should be included in the different...

journal_title：Journal of clinical microbiology

authors： Drancourt M,Bodaghi B,Lepidi H,Le Hoang P,Raoult D

更新日期：2004-04-01 00:00:00

abstract：:Nontuberculous mycobacteria (NTM) cause severe opportunistic infections and have a rising incidence in most settings. Rising diagnostic need must be met by national reference laboratories, which rely on Clinical and Laboratory Standards Institute (CLSI) guideline-approved manual readout of microtiter plates for antimi...

journal_title：Journal of clinical microbiology

authors： Rockland M,Ruth MM,Aalders N,Pennings L,Hoefsloot W,Wattenberg M,van Ingen J

更新日期：2019-03-28 00:00:00

abstract：:The present study describes a method for amplifying DNA in Actinobacillus actinomycetemcomitans by using short, synthetic oligonucleotides of random sequence as primers in the polymerase chain reaction. Genomic DNA from each of 20 human isolates of A. actinomycetemcomitans was successfully amplified in a thermal cycle...

journal_title：Journal of clinical microbiology

authors： Preus HR,Haraszthy VI,Zambon JJ,Genco RJ

更新日期：1993-10-01 00:00:00

abstract：:A procedure for discrimination between scrapie and bovine spongiform encephalopathy (BSE) in sheep is of importance for establishing whether BSE has entered the sheep population. Since BSE has not yet been found in sheep at the farm level, such discrimination procedures can be developed only with experimental sheep BS...

journal_title：Journal of clinical microbiology

authors： Thuring CM,Erkens JH,Jacobs JG,Bossers A,Van Keulen LJ,Garssen GJ,Van Zijderveld FG,Ryder SJ,Groschup MH,Sweeney T,Langeveld JP

更新日期：2004-03-01 00:00:00

abstract：:Streptococcus pneumoniae is both a commensal and a major pathogen that causes invasive disease in people of all ages. The introduction of serotype-specific pneumococcal vaccines has reduced the burden of disease but has also led to replacement with new strains; thus, serotyping remains important for vaccine-related di...

journal_title：Journal of clinical microbiology

authors： Pholwat S,Sakai F,Turner P,Vidal JE,Houpt ER

更新日期：2016-07-01 00:00:00

abstract：:A modified multiple-locus variable-number tandem-repeat analysis (MMLVA) method was validated on Clostridium difficile-infected stool specimens from institutional outbreaks. The method allows simultaneous detection of toxin genes, deletions, and tandem repeats from cultured isolates or stool specimens. Results were us...

journal_title：Journal of clinical microbiology

authors： Broukhanski G,Low DE,Pillai DR

更新日期：2011-05-01 00:00:00

abstract：:Antibody responses of 85 patients to human immunodeficiency virus type 1 antigens were quantitated by densitometric analysis of Western blot (immunoblot) assays. All patients had been classified into the following three clinical categories: asymptomatic (ASY), acquired immunodeficiency syndrome (AIDS)-related complex ...

journal_title：Journal of clinical microbiology

authors： Schmidt G,Amiraian K,Frey H,Wethers J,Stevens RW,Berns DS

更新日期：1989-05-01 00:00:00

abstract：:To detect Streptococcus pneumoniae colonization, the nasopharynx is sampled using a swab placed in skim milk-tryptone-glucose-glycerol (STGG) transport medium, and then the swab specimen or STGG medium is cultured or subjected to PCR. We evaluated the effect of swab composition and compared the sensitivities of detect...

journal_title：Journal of clinical microbiology

authors： Rubin LG,Rizvi A,Baer A

更新日期：2008-08-01 00:00:00

abstract：:A toxic shock syndrome toxin (TSST) variant with an isoelectric point (pI) of 8.6 produced by an ovine-associated Staphylococcus aureus strain was described previously. Analysis of additional strains associated with sheep, goats, cows, and humans by isoelectric focusing with immunoblotting using monoclonal antibodies ...

journal_title：Journal of clinical microbiology

authors： Ho G,Campbell WH,Bergdoll MS,Carlson E

更新日期：1989-09-01 00:00:00

abstract：:Pulsed-field gel electrophoresis revealed that multiple consecutive isolates of Legionella pneumophila from two cardiac transplant patients remained genomically stable, despite exposure to host defenses and antimicrobial agents. ...

journal_title：Journal of clinical microbiology

authors： Marrie TJ,Johnson WM,Tyler SD,Bezanson GS,Burbridge S

更新日期：1994-12-01 00:00:00

abstract：:A new molecular biological approach for the identification of bacteria is described. This approach employs PCR of bacterial cell lysates with conserved primers located in the 16S rRNA sequence flanking a variable region, and analysis of the amplified product was based on the principle of single-strand conformation pol...

journal_title：Journal of clinical microbiology

authors： Widjojoatmodjo MN,Fluit AC,Verhoef J

更新日期：1994-12-01 00:00:00

abstract：:The Tigecycline Evaluation and Surveillance Trial (TEST) was designed to monitor global longitudinal changes in bacterial susceptibility to a panel of antimicrobial agents, including tigecycline. In this study, we examine susceptibility among Gram-positive isolates collected from pediatric patients globally between 20...

journal_title：Journal of clinical microbiology

authors： Brandon M,Dowzicky MJ

更新日期：2013-07-01 00:00:00

abstract：:The fortuitous finding that Aeromonas spp. grew well on Butzler Campylobacter selective medium (BCSA) in a microaerobic atmosphere at 42 degrees C prompted us to evaluate the performance of BCSA for selective isolation of aeromonads in comparison with ampicillin (30 micrograms/ml) sheep blood agar (ASBA30). Overall re...

journal_title：Journal of clinical microbiology

authors： Misra SK,Bhadra RK,Pal SC,Nair GB

更新日期：1989-02-01 00:00:00

abstract：:Acrophialophora fusispora is an emerging opportunistic fungus capable of causing human infections. The taxonomy of the genus is not yet resolved and, in order to facilitate identification of clinical specimens, we have studied a set of clinical and environmental Acrophialophora isolates by morphological and molecular ...

journal_title：Journal of clinical microbiology

authors： Sandoval-Denis M,Gené J,Sutton DA,Wiederhold NP,Guarro J

更新日期：2015-05-01 00:00:00

abstract：:Immunochromatographic test (ICT) kits for the rapid detection of immunoglobulin G (IgG) and IgM antibodies to Burkholderia pseudomallei were compared to the indirect hemagglutination (IHA) assay. In 138 culture-confirmed melioidosis cases, sensitivities were 80, 77, and 88% for IHA, ICT IgG, and ICT IgM, respectively....

journal_title：Journal of clinical microbiology

authors： O'Brien M,Freeman K,Lum G,Cheng AC,Jacups SP,Currie BJ

更新日期：2004-05-01 00:00:00

abstract：:Taeniasis due to Taenia solium is a disease with important public health consequences, since the larval stage is not exclusive to the animal intermediate, the pig, but also infects humans, causing neurocysticercosis. Early diagnosis and treatment of T. solium tapeworm carriers is important to prevent human cysticercos...

journal_title：Journal of clinical microbiology

authors： Mayta H,Gilman RH,Prendergast E,Castillo JP,Tinoco YO,Garcia HH,Gonzalez AE,Sterling CR,Cysticercosis Working Group in Peru.

更新日期：2008-01-01 00:00:00

abstract：:The pathogenesis of human rhinovirus (HRV) during severe respiratory disease remains undefined; thus, we aimed to explore the relationship between the HRV molecular subtyping results obtained during severe and asymptomatic childhood infections. Nasopharyngeal/oropharyngeal swabs from children (1 to 59 months of age) h...

journal_title：Journal of clinical microbiology

authors： Baillie VL,Moore DP,Mathunjwa A,Morailane P,Simões EAF,Madhi SA

更新日期：2019-08-26 00:00:00

abstract：:Diagnosis of invasive aspergillosis (IA) requires increasingly rapid molecular methods that enable sensitive detection and discrimination between species. We designed and evaluated a real-time PCR-based method that combined melting temperature (T(m)) calling analysis of a specific probe with high-resolution melting an...

journal_title：Journal of clinical microbiology

authors： Alonso M,Escribano P,Guinea J,Recio S,Simon A,Peláez T,Bouza E,García de Viedma D

更新日期：2012-10-01 00:00:00

abstract：:The PGMY-PCR for human papillomavirus (HPV) was evaluated, in parallel with nested PCR (nPCR), in samples with noted Hybrid Capture II (HCII) and MY-PCR results. PGMY-PCR detected HPV DNA in 2.5% of HCII-negative-MY-PCR-negative samples and in 71.7% of HCII-positive-MY-PCR-negative samples; also, it detected the MY-PC...

journal_title：Journal of clinical microbiology

authors： Giovannelli L,Lama A,Capra G,Giordano V,Aricò P,Ammatuna P

更新日期：2004-08-01 00:00:00

abstract：:A total of 141 independent strains of Pseudomonas aeruginosa with different heterogeneities in the exo gene (exoS, exoT, exoU, and exoY) background were examined for their pathogenic roles. Results indicated that the exoU gene is the major contributor to cytotoxicity in Madin-Darby canine kidney cells but is not relat...

journal_title：Journal of clinical microbiology

authors： Lin HH,Huang SP,Teng HC,Ji DD,Chen YS,Chen YL

更新日期：2006-12-01 00:00:00

abstract：:The increasing risk of Rift Valley fever virus (RVFV) infection as a global veterinary and public health threat demands the development of safe and accurate diagnostic tests. The aim of this study was to assess the suitability of a baculovirus expression system to produce recombinant RVFV nucleoprotein (N) for use as ...

journal_title：Journal of clinical microbiology

authors： Faburay B,Wilson WC,Secka A,Drolet B,McVey DS,Richt JA

更新日期：2019-09-24 00:00:00

abstract：:GB virus C (GBV-C) markers were analyzed in two to three generations in three families with documented vertical transmission of GBV-C. None of the maternal grandparents had GBV-C markers, whereas the male spouses had GBV-C envelope 2 antibodies. Evidence was found for intrafamilial transmission but not for GBV-C trans...

journal_title：Journal of clinical microbiology

authors： Chen M,Fischler B,Hultgren C,Halasz R,Nemeth A,Sällberg M

更新日期：1999-12-01 00:00:00

abstract：:Strains of the Beijing genotype family of Mycobacterium tuberculosis are a cause of particular concern because of their increasing dissemination in the world and their association with drug resistance. Phylogenetically, this family includes distinct ancient and modern sublineages. The modern strains, contrary to the a...

journal_title：Journal of clinical microbiology

authors： Ribeiro SC,Gomes LL,Amaral EP,Andrade MR,Almeida FM,Rezende AL,Lanes VR,Carvalho EC,Suffys PN,Mokrousov I,Lasunskaia EB

更新日期：2014-07-01 00:00:00

abstract：:Lipopolysaccharide components 3-deoxy-D-manno-2-octulosonic acid and L-glycero-D-manno-heptose were detected in hydrolysates from whole cells of Neisseria elongata by gas-liquid chromatography. Cells from a single plate were hydrolyzed, and carbohydrate components were converted to aldononitrile and O-methyloxime acet...

journal_title：Journal of clinical microbiology

authors： Guerrant GO,Moss CW

更新日期：1986-03-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay for the detection of Shiga toxins (Premier EHEC assay; Meridian Diagnostics, Inc.) was compared to conventional sorbitol-MacConkey culture for the recovery of enterohemorrhagic Escherichia coli. A total of 74 enteric pathogens, including 8 E. coli O157:H7 isolates, were recovered f...

journal_title：Journal of clinical microbiology

authors： Kehl KS,Havens P,Behnke CE,Acheson DW

更新日期：1997-08-01 00:00:00

abstract：:Pneumonia has been identified as a major cause of poor lamb survival in indigenous herds of Rocky Mountain bighorn sheep (Ovis canadensis canadensis) in central Idaho. Pasteurella haemolytica was isolated from five adult Rocky Mountain bighorn ewes captured from a free-ranging herd in central Idaho. The lambs from two...

journal_title：Journal of clinical microbiology

authors： Jaworski MD,Ward AC,Hunter DL,Wesley IV

更新日期：1993-04-01 00:00:00

abstract：:Of 12,321 stool samples analyzed over a 6-year interval, 870 (7.1%) were positive for a total of 1,019 parasites, of which 1,011 (99.2%) were found in trichrome-stained smears of unconcentrated specimens while only 479 (47.0%) were detected in iodine-stained smears of concentrated samples. Stool specimens were next an...

journal_title：Journal of clinical microbiology

authors： Kellogg JA,Elder CJ

更新日期：1999-03-01 00:00:00

abstract：:The Cobas-Bact (Roche Diagnostics, Basel, Switzerland) new rotor for the identification (ID) in 4 h 20 min of 33 members of the family Enterobacteriaceae to genus and species level was evaluated by testing 444 strains of which 398 belonged to common species and 46 belonged to rare species of Enterobacteriaceae. Each s...

journal_title：Journal of clinical microbiology

authors： Wenger A,Tissières LJ,Praplan FR,Kamm WR,Bille J

更新日期：1987-01-01 00:00:00

abstract：:A total of 272 staphylococcal isolates from cases of bovine mastitis (159 Staphylococcus aureus) belonging to 12 different species were identified with ID32 STAPH galleries, and 51 of them were confirmed by 16S rRNA gene (rrs) sequencing. The same isolates were examined for their hemolytic activity on sheep blood agar...

journal_title：Journal of clinical microbiology

authors： Boerlin P,Kuhnert P,Hüssy D,Schaellibaum M

更新日期：2003-02-01 00:00:00