Biochemical markers of the penicillin-induced L phase of a group B, type III Streptococcus sp.

Abstract:

:The penicillin-induced L phase of growth of the group B, type III streptococcal strain 76-043 was examined for biochemical properties used for the identification of group B streptococci. After numerous serial subcultures in the cell wall-defective state, this stable L phase continued to produce hemolysin, hippuricase, and CAMP factor in addition to the group- and type-specific antigens. Hemolysin production by the L-phase cells was observed on solid and in liquid media containing sheep erythrocytes. Washed whole L-phase cells hydrolyzed hippuric acid. CAMP factor was detected by the characteristic hemolysis produced on blood agar by L-phase cells or filtered culture supernatants. CAMP factor activity was quantitated by an agar well diffusion system and a macrotube assay with partially purified preparations of CAMP factor and staphylococcal beta-hemolysin. Hyperimmune cow serum neutralized the CAMP activity of the L phase and parent bacterial phase to the same degree, suggesting identity of the CAMP factors. Production of hemolysin, hippuricase, and CAMP factor confirmed the bacterial origin of this L phase. Assay for these biological markers could be used to identify L-phase organisms derived from group B streptococci.

journal_name

J Clin Microbiol

authors

Flores AE,Ferrieri P

doi

10.1128/JCM.18.4.961-967.1983

subject

Has Abstract

pub_date

1983-10-01 00:00:00

pages

961-7

issue

4

eissn

0095-1137

issn

1098-660X

journal_volume

18

pub_type

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