Defined medium simulating genital tract secretions for growth of vaginal microflora.

abstract：:Antiserum to a purified type R lipopolysaccharide antigen isolated from Neisseria gonorrhoeae was used in a slide agglutination test and compared with conventional carbohydrate utilization and fluorescent antibody tests to confirm the identity of laboratory cultures classified as typical or "atypical" N. gonorrhoeae. ...

journal_title：Journal of clinical microbiology

authors： Arko RJ,Wong KH,Peacock WL

更新日期：1979-04-01 00:00:00

abstract：:Comprehensive data on the prevalence of quinolone resistance in Mycobacterium tuberculosis clinical isolates in the United States are scarce. By use of a systematic population-based approach, M. tuberculosis strains from tuberculosis (TB) cases were collected in Harris County, TX, in 2007 to 2008. The susceptibilities...

journal_title：Journal of clinical microbiology

authors： El Sahly HM,Teeter LD,Jost KC Jr,Dunbar D,Lew J,Graviss EA

更新日期：2011-08-01 00:00:00

abstract：:Discovery of bats with severe acute respiratory syndrome (SARS)-related coronaviruses (CoVs) raised the specter of potential future outbreaks of zoonotic SARS-CoV-like disease in humans, which largely went unheeded. Nevertheless, the novel SARS-CoV-2 of bat ancestral origin emerged to infect humans in Wuhan, China, in...

journal_title：Journal of clinical microbiology

authors： Saif LJ,Jung K

更新日期：2020-07-23 00:00:00

abstract：:A swine H3N2 (swH3N2) and pandemic (H1N1) 2009 (pH1N1) influenza A virus reassortant (swH3N2/pH1N1) was detected in Canadian swine at the end of 2010. Simultaneously, a similar virus was also detected in Canadian mink based on partial viral genome sequencing. The origin of the new swH3N2/pH1N1 viral genes was related ...

journal_title：Journal of clinical microbiology

authors： Tremblay D,Allard V,Doyon JF,Bellehumeur C,Spearman JG,Harel J,Gagnon CA

更新日期：2011-12-01 00:00:00

abstract：:We evaluated a newly commercial enzyme immunoassay (EIA) (Biotest Legionella Urin Antigen EIA; Biotest AG, Dreieich, Germany) for detection of antigens of all Legionella pneumophila serogroups with a relatively wide spectrum of cross-reactivity as well as antigens of other Legionella spp. by comparing its sensitivity ...

journal_title：Journal of clinical microbiology

authors： Domínguez JA,Galí N,Pedroso P,Fargas A,Padilla E,Manterola JM,Matas L

更新日期：1998-09-01 00:00:00

abstract：:Helicobacter pylori strains possessing the cag pathogenicity island (PaI) are associated with the development of gastroduodenal diseases, including gastric cancer. cag PaI products induce the secretion of interleukin-8 (IL-8) from epithelial cells and facilitate the translocation of CagA into the cell cytosol. In East...

journal_title：Journal of clinical microbiology

authors： Zhang Y,Argent RH,Letley DP,Thomas RJ,Atherton JC

更新日期：2005-02-01 00:00:00

abstract：:The human metapneumovirus (hMPV) was recently identified and linked to acute respiratory tract infections (ARTI). To assess the clinical importance of this virus in infants and children, we developed a rapid and efficient reverse transcription-PCR-based screening method for a large volume of samples and tested retrosp...

journal_title：Journal of clinical microbiology

authors： Chano F,Rousseau C,Laferrière C,Couillard M,Charest H

更新日期：2005-11-01 00:00:00

abstract：:In this work, we show that antibodies to baculovirus recombinant DNA polymerase of human cytomegalovirus are present in human sera during natural infection. Specific antibody was found to be of the immunoglobulin G class, produced at low titers only for a short period of time at the beginning of acute infection and no...

journal_title：Journal of clinical microbiology

authors： Landini MP,Lazzarotto T,Ertl PF

更新日期：1993-03-01 00:00:00

abstract：:To our knowledge, no data are available on whether the microbial species composition and abundance sampled with self-collected vaginal swabs are comparable to those of swabs collected by clinicians. Twenty healthy women were recruited to the study during a routine gynecological visit. Eligible women were between 18 an...

journal_title：Journal of clinical microbiology

authors： Forney LJ,Gajer P,Williams CJ,Schneider GM,Koenig SS,McCulle SL,Karlebach S,Brotman RM,Davis CC,Ault K,Ravel J

更新日期：2010-05-01 00:00:00

abstract：:We compared the Gen-Probe transcription-mediated amplification assay (AMP CT), the Abbott LCx assay, and the Roche COBAS AMPLICOR assay for the detection of Chlamydia trachomatis in a mixed population in urine samples. First-void urine, urethral specimens, and cervical specimens in females were obtained from 1,000 pat...

journal_title：Journal of clinical microbiology

authors： Goessens WH,Mouton JW,van der Meijden WI,Deelen S,van Rijsoort-Vos TH,Lemmens-den Toom N,Verbrugh HA,Verkooyen RP

更新日期：1997-10-01 00:00:00

abstract：:The new commercial assay GenoType Mycobacterium, intended for the identification of mycobacteria, was evaluated with a panel of 197 strains belonging to 86 different taxa. The system, which relies on solid-phase reverse hybridization, is composed of two different kits: GenoType CM for the identification of more freque...

journal_title：Journal of clinical microbiology

authors： Russo C,Tortoli E,Menichella D

更新日期：2006-02-01 00:00:00

abstract：:Based on cultural and biochemical tests, a total of 84 strains (72 clinical and 12 environmental isolates from the Caribbean Isles, Europe, and the Indian subcontinent) were identified as members of the Mycobacterium avium complex (MAC). They were further characterized with MAC, M. avium, and M. intracellulare probes ...

journal_title：Journal of clinical microbiology

authors： Devallois A,Picardeau M,Paramasivan CN,Vincent V,Rastogi N

更新日期：1997-11-01 00:00:00

abstract：:IS6110 restriction fragment length polymorphism (RFLP) genotyping is the most widely used genotyping method to study the epidemiology of Mycobacterium tuberculosis. However, due to the complexity of the IS6110 RFLP genotyping technique, and the interpretation of RFLP data, mycobacterial interspersed repetitive-unit-va...

journal_title：Journal of clinical microbiology

authors： Hanekom M,van der Spuy GD,Gey van Pittius NC,McEvoy CR,Hoek KG,Ndabambi SL,Jordaan AM,Victor TC,van Helden PD,Warren RM

更新日期：2008-10-01 00:00:00

abstract：:A 10-min latex agglutination test kit, Culturette Brand Ten-Minute Group A Strep ID (Marion Scientific, Div. of Marion Laboratories, Inc., Kansas City, Mo.), was assessed for the rapid detection of group A beta-hemolytic streptococcus directly from a throat swab. Four hundred and thirty-five throat swab specimens from...

journal_title：Journal of clinical microbiology

authors： Chang MJ,Mohla C

更新日期：1985-02-01 00:00:00

abstract：:Three cases of neonatal infection caused by Enterobacter sakazakii are reported from the Department of Neonatal Intensive Care, the National University Hospital, Reykjavík, Iceland. These infections occurred during a 9-month period in 1986 and 1987. Two of the neonates, who were normal at birth, survived but were left...

journal_title：Journal of clinical microbiology

authors： Biering G,Karlsson S,Clark NC,Jónsdóttir KE,Lúdvígsson P,Steingrímsson O

更新日期：1989-09-01 00:00:00

abstract：:The appearance and persistence of immunoglobulin M (IgM) and IgG antibodies in postnatal rubella infections were studied by employing a solid-phase radioimmunoassay test. Altogether, 222 serial serum specimens from 51 patients with acute rubella infection were tested. Both IgG and IgM antibodies developed rapidly and ...

journal_title：Journal of clinical microbiology

authors： Meurman OH

更新日期：1978-01-01 00:00:00

abstract：:Recovery of aerobic and anaerobic bacteria from clinical specimens maintained in the Copan Venturi Transystem and the Becton Dickinson Port-a-Cul transport was assessed. Of 54 anaerobes, 53 were recovered after 4 h, and 52 were recovered after 24 h, from both systems. After 48 h, 45 and 50 were recovered from the two ...

journal_title：Journal of clinical microbiology

authors： Citron DM,Warren YA,Hudspeth MK,Goldstein EJ

更新日期：2000-02-01 00:00:00

abstract：:The detection and successful typing of dengue virus (DENV) from patients with suspected dengue fever is important both for the diagnosis of the disease and for the implementation of epidemiologic control measures. A technique for the multiplex detection and typing of DENV serotypes 1 to 4 (DENV-1 to DENV-4) from clini...

journal_title：Journal of clinical microbiology

authors： Das S,Pingle MR,Muñoz-Jordán J,Rundell MS,Rondini S,Granger K,Chang GJ,Kelly E,Spier EG,Larone D,Spitzer E,Barany F,Golightly LM

更新日期：2008-10-01 00:00:00

abstract：:Azole-resistant Aspergillus fumigatus is an increasing worldwide problem with major clinical implications. Surveillance is warranted to guide clinicians to provide optimal treatment to patients. To investigate azole resistance in clinical Aspergillus isolates in our institution, a Belgian university hospital, we condu...

journal_title：Journal of clinical microbiology

authors： Montesinos I,Argudín MA,Hites M,Ahajjam F,Dodémont M,Dagyaran C,Bakkali M,Etienne I,Jacobs F,Knoop C,Patteet S,Lagrou K

更新日期：2017-08-01 00:00:00

abstract：:We have shown that a modification of the color-coded antigen developed by Kasatiya and Lambert (Appl. Microbiol. 28:317-318, 1974) can be used as a substitute for other nontreponemal antigens used in screening tests for syphilis. The antigen is based on the Venereal Disease Research Laboratory antigen, with EDTA, chol...

journal_title：Journal of clinical microbiology

authors： Pettit DE,Larsen SA,Harbec PS,Feeley JC,Parham CE,Cruce DD,Hambie EA,Perryman MW

更新日期：1983-11-01 00:00:00

abstract：:We have used comparative genomic hybridization (CGH) on a full-genome Campylobacter jejuni microarray to examine genome-wide gene conservation patterns among 51 strains isolated from food and clinical sources. These data have been integrated with data from three previous C. jejuni CGH studies to perform a meta-analysi...

journal_title：Journal of clinical microbiology

authors： Taboada EN,Acedillo RR,Carrillo CD,Findlay WA,Medeiros DT,Mykytczuk OL,Roberts MJ,Valencia CA,Farber JM,Nash JH

更新日期：2004-10-01 00:00:00

abstract：:Fifty staphylococcal strains that produced toxic shock syndrome (TSS) toxin 1 and that were isolated from patients with TSS were characterized. One strain had more properties that were characteristic of Staphylococcus hyicus than of Staphylococcus aureus. Forty-four strains had the same properties or differed in only ...

journal_title：Journal of clinical microbiology

authors： Aliu B,Bergdoll MS

更新日期：1988-11-01 00:00:00

abstract：:Roche Molecular Systems recently released two PCR-based assays, AMPLICOR and LINEAR ARRAY (LA), for the detection and genotyping, respectively, of human papillomaviruses (HPVs). The manual specimen processing method recommended for use with both assays, AmpliLute, can be time-consuming and labor-intensive and is open ...

journal_title：Journal of clinical microbiology

authors： Stevens MP,Rudland E,Garland SM,Tabrizi SN

更新日期：2006-07-01 00:00:00

abstract：:We describe a Klebsiella oxytoca infection outbreak in a renal transplant unit that involved seven patients. All strains belonged to a single pulsed-field gel electrophoresis pattern and were resistant to amoxicillin-clavulanate, cefuroxime, piperacillin-tazobactam, and aztreonam but susceptible to ceftriaxone, ceftaz...

journal_title：Journal of clinical microbiology

authors： Zárate MS,Gales AC,Picão RC,Pujol GS,Lanza A,Smayevsky J

更新日期：2008-06-01 00:00:00

abstract：:Human adenoviruses (AdVs) typically cause mild illnesses in otherwise healthy hosts. We investigated a pediatric outbreak of acute respiratory infection with fatal outcomes that occurred in Lisbon, Portugal, in 2004. Biological specimens were collected from 83 children attending two nurseries, a kinesiotherapy clinic,...

journal_title：Journal of clinical microbiology

authors： Rebelo-de-Andrade H,Pereira C,Gíria M,Prudêncio E,Brito MJ,Calé E,Taveira N

更新日期：2010-04-01 00:00:00

abstract：:Class-specific enzyme-linked immunosorbent assays (ELISAs) with purified recombinant antigens of Borrelia burgdorferi sensu stricto and Western blot analyses with whole cells of this spirochete were used to test human sera to determine which antigens were diagnostically important. In analyses for immunoglobulin M (IgM...

journal_title：Journal of clinical microbiology

authors： Magnarelli LA,Ijdo JW,Padula SJ,Flavell RA,Fikrig E

更新日期：2000-05-01 00:00:00

abstract：:A reverse transcription-PCR (RT-PCR) assay based on automated fluorescent capillary electrophoresis and GeneScan software analysis was developed to detect six common respiratory viruses in clinical specimens from young children. Assays for human respiratory syncytial virus (HRSV); human parainfluenza viruses 1, 2, and...

journal_title：Journal of clinical microbiology

authors： Erdman DD,Weinberg GA,Edwards KM,Walker FJ,Anderson BC,Winter J,González M,Anderson LJ

更新日期：2003-09-01 00:00:00

abstract：:We report a case in which fecal microbiota transplantation (FMT) utilized for relapsing Clostridium difficile colitis successfully eradicated colonization with several multidrug-resistant organisms (MDROs). FMT may have an additive benefit of reducing MDRO carriage and should be further investigated as a potential mea...

journal_title：Journal of clinical microbiology

authors： Crum-Cianflone NF,Sullivan E,Ballon-Landa G

更新日期：2015-06-01 00:00:00

abstract：:The results of anaerobic susceptibility tests performed with inocula prepared directly from agar isolation media and from overnight broth cultures were compared. Altogether, 93.0% of the results with these two inoculum preparations were within one twofold dilution, including 92.5 and 95.6% of the results with Clostrid...

journal_title：Journal of clinical microbiology

authors： Murray PR,Niles AC

更新日期：1983-09-01 00:00:00

abstract：:The performance of a commercially available, rapid membrane enzyme immunoassay for influenza A and B virus detection was compared to that of viral culture in 4,092 respiratory specimens collected from patients presenting with respiratory symptoms during the 2002-2003 influenza season. The test's overall sensitivity wa...

journal_title：Journal of clinical microbiology

authors： Cazacu AC,Chung SE,Greer J,Demmler GJ

更新日期：2004-08-01 00:00:00